Homogenization, Extraction, Stability and Testing by LC-UV of Psychoactive Alkaloids Found in Psychedelic Mushrooms

Microdosing is the practice of regularly administering very low doses of a psychoactive substance. Recent studies have indicated microdosing shows promise for the management and treatment of anxiety, depression, and other mental health disorders. Emerging research has been focusing on microdosing psilocin and psilocybin, both of which are frequently found in psychedelic mushrooms. With recent legalization of psilocybin and psilocin in certain areas of the United States, there is a growing interest among research and production labs to understand more about the stability and testing of these compounds. This study examines four distinct strains of psilocybe mushrooms, focusing on the processes of homogenization, extraction efficiency, and extract stability. Primary analysis was performed using LC-UV. A total of seven psychoactive alkaloids commonly found in psychedelic mushrooms were monitored using a simple gradient on a Force Inert Biphenyl column with a total cycle time of eight minutes.