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PFAS Analysis by DIN 38407-42 for Water, Wastewater, and Sludge

03 Oct 2025

feature EVAR4299
  • Sample Matrix: water, wastewater, and sludge
  • Sample Preparation: WAX solid-phase extraction
  • Detection Technique: LC-MS/MS

Determination of selected polyfluorinated compounds (PFC) in water-Method using high-performance liquid chromatography and mass spectrometric detection (HPLC/MS-MS) after solid-liquid extraction

DIN 38407-42 is used for the analysis of 10 PFAS in water, wastewater, and sludge. It is not available from Restek but may be purchased from other sources. The full analyte list and recommended products for DIN 38407-42 are provided below. In addition, you can register and directly compare analyte lists and matrices across PFAS analysis methods here or visit www.restek.com/PFAS to explore our technical resources for PFAS analysis.

Analyte List for DIN 38407-42 for PFAS Analysis

PFAS acronyms, compound names, and CAS numbers vary across methods and guidelines, and they also depend on compound form (e.g., neutral vs. salt). The following list uses standardized terminology to allow easy comparison across the methods and guidelines in our comprehensive guide. Please refer to DIN 38407-42 for method-specific nomenclature.

AcronymCompound NameCAS#
PFBSPerfluoro-1-butanesulfonic acid375-73-5
PFDAPerfluorodecanoic acid335-76-2
PFHpAPerfluoroheptanoic acid375-85-9
PFHxSPerfluoro-1-hexanesulfonic acid355-46-4
PFHxAPerfluorohexanoic acid307-24-4
PFNAPerfluorononanoic acid375-95-1
PFOSPerfluorooctanesulfonic acid1763-23-1
PFOAPerfluorooctanoic acid335-67-1
PFBAPerfluorobutanoic acid375-22-4
PFPeAPerfluoropentanoic acid2706-90-3

Recommended Products for DIN 38407-42 for PFAS Analysis

Analytical LC Columns:


Raptor C18, 2.7 µm, 50×2.1 mm HPLC-Säule
Force C18, 1.8 µm, 50 x 2.1mm LC-Säule

Delay Columns:


PFAS Delay-Säule, 5 µm, 50×2.1 mm HPLC-Säule

PFAS delay column: 5 µm, 50 x 3.0 mm (cat.# 580959 [custom product])

Guard Columns:


UltraShield UHPLC Vorsäulen-Filter, 0.2 μm Fritte, 1
EXP Direct Connect-Halter für EXP Vorsäulenkartuschen, enthält Fittings und Ferrules
Raptor C18 EXP Vorsäulenkartusche, 2.7 µm, 5 x 2.1 mm, 3er Pack

Standards:


PFAS 24 Kalibrierstandard, 1 µg/mL, Methanol (1 mM KOH), 1 mL/Ampulle
PFAS 28 Kalibrierstandard, 1 µg/mL, Methanol (1 mM KOH), 1 mL/Ampulle
Discontinued: EPA 533 PFAS Kalibrierstandard, 2 µg/mL, Methanol (1 mM KOH), 1 mL/Ampulle

Sample Prep:


30 mm Spritzenfilter, 0.45 µm, Nylon, pink, 100er Pack
30 mm Spritzenfilter, 0.22 µm, Nylon, pink, 100er Pack
Resprep Polymerische SPE-Kartusche, WCX, 6 mL/500 mg, 30 μm, 30er Pck.
Resprep Polymerische SPE-Kartusche, WCX, 6 mL/200mg, 30 μm, 30er Pck.
SPE Leerröhrchen, Probenreservoir, 75 mL, Polypropylen, 12er Pack
SPE Verbinder, 1, 3, 6, 10 oder 15 mL, Polypropylen, 15er Pack

*Note that a regenerated cellulose filter is mentioned in the method. However, nylon and polypropylene are widely accepted filter membrane materials for PFAS analysis.

Vacuum Manifolds:


Resprep QR-24 Vakuum-Absaugstation
Resprep QR-12 Vakuum-Absaugstation

Vacuum Pumps:


Chemker 300 PTFE Vakuumpumpe, 20 L/min, AC 110 V, 60 Hz
Chemker 300 PTFE Vakuumpumpe, 18 L/min, AC 220 V, 50 Hz

General Lab Supplies:


Screw-Thread Polypropylen Vials mit begrenztem Volumen, 9 mm, 700 μL, 12 x 32 mm, 100er Pack
Screw-Thread Polypropylen Vials mit begrenztem Volumen, 9 mm, 700 μL, 12 x 32 mm, 1000er Pack
2.0 mL, 9 mm Solid Top-Polyethylen Kappen, Screw-Thread, 10 mil dicke Membran, farblos, 100er Pack
2.0 mL, 9 mm Solid Top-Polyethylen Kappen, Screw-Thread, 10 mil dicke Membran, farblos, 1000er Pack
Leere Zentrifugenröhrchen, 50 mL, Polypropylen mit Kappe, 50er Pck.
Leere Zentrifugenröhrchen, 50 mL, Polypropylen mit Kappe, 500er Pck.
Norm-Ject Kunststoffspritze, 10 mL Luer-Lock-Spitze, 100er Pck.
Norm-Ject Kunststoffspritze, 5 mL Luer-Lock-Spitze, 100er Pck.

Discover More Solutions for PFAS Analysis!

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  • Restek Corporation

    Restek is a leading provider of chromatography columns, accessories, and certified reference materials. Trust Restek for reliable, high-quality analytical solutions.

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