Many labs extend the list of compounds tested using Method 8081B to include other organochlorine pesticides. Rtx-CLPesticides and Rtx-CLPesticides2 columns are ideal for these determinations and can be used in a parallel dual-column configuration to reliably quantify 38 commonly analyzed organochlorine pesticides, as well as many other compounds. Full separations are achieved between both columns and the unique, optimized selectivity allows additional compounds to be included. (Note that since extended compound lists vary among laboratories the lists compared here are not identical.)
| Vendor | Column Pair | Analysis Time (min) | Coelutions | Restek Advantage |
|---|---|---|---|---|
| Restek | Rtx-CLPesticides / Rtx-CLPesticides2 | 24 / 23 | 1 / 2 | Best balance of speed and selectivity. All compounds are resolved on at least one column. |
| Competitor A | column 1 / column 2 | 27 / 29 | 0 / 3 | |
| Competitor B | column 1 / column 2 | NDP / 16 | NDP / 3 |
Comparison based on published competitor data. All columns tested were 0.32 mm ID. NDP = no data published
The unique selectivity of the Rtx-CLPesticides column pair allows easy separation of extended lists of organochlorine pesticides.
GC_EV1193
Peaks
| Peaks | Conc. (mg/mL) | |
|---|---|---|
| 1. | 1,2-Dibromo-3-chloropropane | 100 |
| 2. | Hexachloro-cyclopentadiene | 100 |
| 3. | Tetrachloro-m-xylene (SS) | 100 |
| 4. | cis-Diallate | 500 |
| 5. | Hexachlorobenzene | 100 |
| 6. | trans-Diallate | 500 |
| 7. | α-BHC | 40 |
| 8. | γ-BHC | 40 |
| 9. | β-BHC | 40 |
| 10. | δ-BHC | 40 |
| 11. | Heptachlor | 40 |
| 12. | Aldrin | 40 |
| Peaks | Conc. (mg/mL) | |
|---|---|---|
| 13. | Chlorpyrifos | 100 |
| 14. | Isodrin | 100 |
| 15. | Oxychlordane | 50 |
| 16. | Heptachlor epoxide | 40 |
| 17. | trans-Chlordane | 40 |
| 18. | 2,4′-DDE | 100 |
| 19. | trans-Nonachlor | 100 |
| 20. | cis-Chlordane | 40 |
| 21. | Endosulfan I | 40 |
| 22. | Captan | 100 |
| 23. | 4,4′-DDE | 100 |
| 24. | Dieldrin | 80 |
| Peaks | Conc. (mg/mL) | |
|---|---|---|
| 25. | 2,4′-DDD | 100 |
| 26. | Endrin | 80 |
| 27. | Chlorobenzilate | 500 |
| 28. | 2,4′-DDT | 100 |
| 29. | cis-Nonachlor | 100 |
| 30. | 4,4′-DDD | 80 |
| 31. | Endosulfan II | 80 |
| 32. | 4,4′-DDT | 80 |
| 33. | Endrin aldehyde | 80 |
| 34. | Endosulfan sulfate | 80 |
| 35. | Methoxychlor | 400 |
| 36. | Mirex | 100 |
| 37. | Endrin ketone | 80 |
| 38. | Decachloro-biphenyl | 100 |
Conditions
| Columns | Rtx-CLPesticides2 30 m, 0.32 mm ID, 0.25 µm (cat.# 11324) |
|---|---|
| and Rtx-CLPesticides 30 m, 0.32 mm ID, 0.32 µm (cat.# 11141) | |
| using Rxi guard column 5 m, 0.32 mm ID (cat.# 10039) | |
| with deactivated universal “Y” Press-Tight connector (cat.# 20405-261) | |
| Standard/Sample | |
| Organochlorine pesticide mix AB #2 (cat.# 32292) | |
| Organochlorine pesticide mix C #1 (cat.# 32296) | |
| 2,4,5,6-Tetrachloro-m-xylene (cat.# 32027) | |
| Decachlorobiphenyl (BZ #209) (cat.# 32029) | |
| Chlorpyrifos (cat.# 32212) | |
| 2,4′-DDE (cat.# 32099) | |
| 2,4′-DDD (cat.# 32098) | |
| Chlorobenzilate | |
| Captan (custom) | |
| cis-Nonachlor (custom) | |
| Mirex (custom) | |
| Oxychlordone (custom) | |
| Diallate (custom) | |
| Diluent: | Hexane |
| Injection | |
| Inj. Vol.: | 1 µL splitless (hold 0.3 min) |
| Liner: | Cyclo double taper (4 mm) (cat.# 20896) |
| Inj. Temp.: | 250 °C |
| Oven | |
| Oven Temp.: | 80 °C to 175 °C at 8 °C/min to 195 °C at 11 °C/min to 235 °C at 7 °C/min to 320 °C at 25 °C/min (hold 3 min) |
| Carrier Gas | He, constant flow |
| Linear Velocity: | 28 cm/sec |
| Detector | Micro-ECD @ 330 °C |
|---|---|
| Make-up Gas Flow Rate: | 60 mL/min |
| Make-up Gas Type: | N2 |
| Instrument | Agilent/HP6890 GC |
| Notes | This chromatogram was obtained using an Agilent micro-ECD. To obtain comparable results, you will need to employ a micro-ECD in addition to confirmational dual columns connected to a 5-meter guard column using a “Y” Press-Tight connector. Reference standard cat.# 32211 was used to produce this chromatogram, but has since been discontinued. For assistance choosing a replacement for this application, contact Restek Technical Service or your local Restek representative. |
Products Mentioned
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EVAR4500
