Author(s): Chris English
Restek Corporation
Published By: LCGC
Issue: 2, Volume, 21
Year of Publication: 2025
Link: https://www.chromatographyonline.com/view/liner-volume-matters
Abstract: There are many different injection port liner configurations, all of which come in a variety of internal volumes. It has been well established that injecting too much solvent into a liner in splitless mode results in a vapor cloud that exceeds the internal volume of the liner, which then leads to peak distortion, split peaks, and poor reproducibility. High-molecular-weight compounds condense on the cooler surfaces outside of the liner and are not completely transferred onto the column causing carryover (memory effects) and compound bias. This is known as discrimination where an inverse relationship occurs between the molecular weight and response of a compound. Recent studies have demonstrated that injecting too little into a liner in splitless mode can cause band broadening and increased activity. This article will examine these effects in split mode.
