Prior to use, your Raptor HILIC-Si column must be properly conditioned with the mobile phase that will be used during analysis. For isocratic HILIC methods, at least 50 column volumes should be used and, for gradient HILIC methods, at least 10 blank injections running the complete time program should be performed. Conditioning is also required when you change the mobile phase composition or the concentration of any additives. Use the same number of column volumes or blank injections as you did when conditioning the column for the first time. Table I below gives the column volumes for Raptor HILIC-Si columns based on their dimensions.
Table I: Column Volumes (mL) Based upon Raptor HILIC-Si Column Dimensions
|
Column ID |
30 mm Length |
50 mm Length |
100 mm Length |
150 mm Length |
|
2.1 mm |
0.05 |
0.1 |
0.2 |
0.3 |
|
3.0 mm |
— |
0.2 |
0.4 |
0.5 |
|
4.6 mm |
— |
0.4 |
0.8 |
1.2 |
