The HPLC- UV Analysis of Psilocin, Psilocybin, and Others Found in Psychedelic Compound Containing Mushrooms and Infused Products

Psilocin and psilocybin are tryptamine alkaloids found in some plants and fungi. Often found in certain species of mushrooms, these compounds are used for their psychotropic effects, but microdosing has shown promise for the treatment of anxiety, depression, and other mental health disorders. With recent decriminalization and legalization in certain areas, of both naturally derived and synthetic psychedelic compounds, labs are seeking to begin potency testing of psychedelic compound containing mushrooms and infused products. A set of efficient methods were developed for the testing of two psychedelic compound containing mushroom species. Sample preparation and extraction processes were established for infused chocolates, gummies, and extracts, as well as freshly ground mushrooms. Due to the robust nature, an HPLC-UV system was used to collect all data. A method for six compounds endogenous to psychedelic compound containing mushrooms was developed using a Force Biphenyl 100 x 3.0 mm, 3 μm column with a full cycle time of 7.5 minutes. Another method for the analysis of psychedelic compounds endogenous to the Amanita muscaria mushroom, ibotenic acid, and muscimol was developed utilizing an Ultra IBD 30 x 3.0 mm, 3 μm column with a 4.5-minute cycle time.