Comprehensive PFAS Analysis Using Diverse LC Column Types

Growing interest in a wider range of per- and polyfluoroalkyl substances (PFAS) in various matrix types requires substantial optimization in liquid chromatography (LC) for comprehensive analysis. Leveraging the LC platform, our study and review aim to elucidate the advantages and limitations of different column chemistries in the separation and quantification of PFAS compounds, including ultrashort chain, long-chain variants, and sample matrix challenges. The investigation involves systematically comparing reversed-phase, ion-exchange, and mixed-mode chemical interactions to enhance chromatographic resolution and sensitivity. Our findings contribute to advancing the understanding of PFAS analysis, offering insights into the optimal LC column selection for robust and thorough detection of these persistent environmental contaminants.