{"id":42474,"date":"2019-11-12T05:00:00","date_gmt":"2019-11-12T05:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/anforderungen-an-die-injektionslosung-bei-der-hilic-chromatografie\/"},"modified":"2026-01-28T22:20:41","modified_gmt":"2026-01-28T22:20:41","slug":"anforderungen-an-die-injektionslosung-bei-der-hilic-chromatografie","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/de\/videos-de\/gnav3613-de\/anforderungen-an-die-injektionslosung-bei-der-hilic-chromatografie","title":{"rendered":"Anforderungen an die Injektionsl\u00f6sung bei der HILIC-Chromatografie"},"content":{"rendered":"\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"Injection Solution Conditions for HILIC\" width=\"720\" height=\"405\" src=\"https:\/\/www.youtube.com\/embed\/iX3Tm2v9DGU?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Beschreibung<\/h2>\n\n\n\n<p>Bei der HILIC-Chromatografie (\u201eHydrophilic Interaction Liquid Chromatography\u201c) enth\u00e4lt die anf\u00e4ngliche mobile Phase einen hohen organischen Anteil und es ist sehr wichtig, dass Ihre Injektionsl\u00f6sung darin l\u00f6slich ist. In diesem Video erl\u00e4utern wir die Anforderungen an die Injektionsl\u00f6sung f\u00fcr HILIC-Analysen.&nbsp;<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Weitere Informationen<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.restek.com\/articles\/how-to-avoid-common-problems-with-hilic-methods\" target=\"_blank\" rel=\"noopener\">How to Avoid Common Problems with HILIC Methods<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/articles\/raptor-hilic-si-simplify-the-switch-to-hilic\" target=\"_blank\" rel=\"noopener\">Simplify the Switch to HILIC with Raptor HILIC-Si Columns<\/a><\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Von Restek empfohlene Tools &amp; Ersatzteile<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.restek.com\/global\/de\/search\/hilic-si\/_\/N-1673632971\/?Ns=restekProduct.x_productBadge%7C0\" data-type=\"link\" data-id=\"https:\/\/www.restek.com\/global\/de\/search\/hilic-si\/_\/N-1673632971\/?Ns=restekProduct.x_productBadge%7C0\" target=\"_blank\" rel=\"noopener\">Raptor HILIC-Si S\u00e4ulen<\/a><\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Transkript<\/strong><\/h2>\n\n\n\n<p>Wenn Sie bereits Erfahrung in der Entwicklung von Umkehrphasen-LC-Methoden haben, wissen Sie, dass die Injektionsl\u00f6sung einen gro\u00dfen Einfluss auf die Peakformen haben kann, besonders bei fr\u00fch eluierenden Analyten. Dieser Einfluss ist bei HILIC LC-Analysen noch wesentlich ausgepr\u00e4gter. Es ist sehr wichtig, die Injektionsl\u00f6sung dem anf\u00e4nglichen Zustand der mobilen Phase anzupassen, die einen hohen organischen Anteil besitzt. Wie das vorliegende Beispiel zeigt, ergab die Analyse von Diquat und Paraquat auf einer Raptor HILIC-Kieselgels\u00e4ule ein Chromatogramm mit besserer Peakform, h\u00f6herer Retention und h\u00f6herer Empfindlichkeit, wenn die Probe anstatt in einer 100%ig w\u00e4ssrigen L\u00f6sung in einer L\u00f6sung mit 75 % organischem Anteil gel\u00f6st wurde, die dem Anfangszustand der mobilen Phase entsprach.<\/p>\n\n\n\n<p>Zusammenfassend l\u00e4sst sich sagen, dass HILIC-Methoden eine leistungsf\u00e4hige M\u00f6glichkeit zur Analyse polarer Verbindungen bieten, die sich mit Umkehrphasen-LC-Methoden nicht erreichen l\u00e4sst. Sie m\u00fcssen lediglich daf\u00fcr sorgen, dass die S\u00e4ule vor und zwischen Injektionen korrekt konditioniert und wieder \u00e4quilibriert wird, dass die Auswirkungen des pH-Werts der mobilen Phase und die Auswahl des Puffers auf Ihre Analyse ber\u00fccksichtigt werden und dass die Injektionsl\u00f6sung dem Anfangszustand der mobilen Phase angepasst wird. Diese Richtlinien gelten f\u00fcr alle station\u00e4ren HILIC-Phasen, mit denen Sie arbeiten. Wenn Ihnen diese Videos gefallen, denken Sie bitte an die \u201eGef\u00e4llt mir\u201c-Angabe und teilen und abonnieren Sie sie. Sie k\u00f6nnen das nachstehende Kommentarfeld verwenden, um uns Ihre Ideen f\u00fcr k\u00fcnftige TechTipps mitzuteilen.<\/p>\n\n\n\n<p>Wir w\u00fcnschen Ihnen viel Erfolg bei der Entwicklung Ihrer eigenen HILIC LC-Methode.<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Verstehen Sie Ihre Injektionsl\u00f6sung, wenn Sie HILIC-Analysen durchf\u00fchren.<\/p>\n","protected":false},"author":11,"featured_media":41133,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[772],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[2481],"resource-technique":[2355],"ppma_author":[414],"class_list":["post-42474","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-videos-de","product-library-flussigchromatographie-produkte","resource-technique-hilic-de"],"acf":[],"taxonomy_info":{"category":[{"value":772,"label":"Videos"}],"product-library":[{"value":2481,"label":"Fl\u00fcssigchromatographie Produkte"}],"resource-technique":[{"value":2355,"label":"HILIC"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-GNAV3613-1024x536.jpg",1024,536,true],"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/de\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":772,"name":"Videos","slug":"videos-de","term_group":0,"term_taxonomy_id":772,"taxonomy":"category","description":"","parent":0,"count":133,"filter":"raw","cat_ID":772,"category_count":133,"category_description":"","cat_name":"Videos","category_nicename":"videos-de","category_parent":0}],"tag_info":false,"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/42474","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/comments?post=42474"}],"version-history":[{"count":5,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/42474\/revisions"}],"predecessor-version":[{"id":85781,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/42474\/revisions\/85781"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/media\/41133"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/media?parent=42474"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/categories?post=42474"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/tags?post=42474"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/industries-application?post=42474"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/post-badge?post=42474"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-type?post=42474"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/product-library?post=42474"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-technique?post=42474"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/ppma_author?post=42474"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}