{"id":42491,"date":"2018-01-03T05:00:00","date_gmt":"2018-01-03T05:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/restek-tipp-lc-fehlersuche-optimierung-der-injektion-zur-verbesserung-der-peakform\/"},"modified":"2026-01-27T22:32:39","modified_gmt":"2026-01-27T22:32:39","slug":"restek-tipp-lc-fehlersuche-optimierung-der-injektion-zur-verbesserung-der-peakform","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/de\/videos-de\/gnav3621-de\/restek-tipp-lc-fehlersuche-optimierung-der-injektion-zur-verbesserung-der-peakform","title":{"rendered":"Restek Tipp: LC Fehlersuche &#8211; Optimierung der Injektion zur Verbesserung der Peakform"},"content":{"rendered":"\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"LC Troubleshooting\u2014Optimizing Your Injection to Improve Peak Shape\" width=\"720\" height=\"405\" src=\"https:\/\/www.youtube.com\/embed\/GDfsNH4qbcE?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Beschreibung<\/h2>\n\n\n\n<p>Injection volume and solvent choice are among the many factors that contribute to good liquid chromatography. If we inject too much, we can drastically degrade peak shape by causing our peaks to front. At the same time, we want to choose an injection solvent that&nbsp;matches&nbsp;our initial mobile phase conditions.&nbsp;<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Weitere Informationen<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/discover.restek.com\/tag\/faq-hplc-and-uhplc\/\" target=\"_blank\" rel=\"noreferrer noopener\">HPLC and UHPLC Frequently Asked Questions<\/a><\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Von Restek empfohlene Tools &amp; Ersatzteile<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.restek.com\/search\/syringes-accessories\/_\/N-1447637172?Ntt=LC&amp;Ns=bestMatch\" target=\"_blank\" rel=\"noreferrer noopener\">LC Syringes<\/a><\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Transkript<\/h2>\n\n\n\n<p>Vielen Dank f\u00fcr Ihr Interesse an unserem aktuellen Restek Tipp. Heute m\u00f6chte ich dar\u00fcber sprechen, wie das Injektionsvolumen und die Wahl des L\u00f6semittels die Peakform beeinflussen k\u00f6nnen. Wie wir wissen, gibt es in der Fl\u00fcssigchromatografie eine Reihe von Faktoren, die an der Entstehung schlechter Peakformen beteiligt sein k\u00f6nnen. Dabei sind das Injektionsvolumen und die Wahl des L\u00f6semittels zwei Dinge, die wir uns ansehen k\u00f6nnen, ohne viele andere chromatografische Parameter zu \u00e4ndern.<\/p>\n\n\n\n<p>In Bezug auf das Injektionsvolumen gilt als gute Faustregel, dass es zwischen 1 und 5 % des Gesamtvolumens der S\u00e4ule betragen sollte. Wenn wir 5 % \u00fcberschreiten, sehen wir erste Anzeichen von Peakfronting, was bedeutet, dass wir uns der maximalen Kapazit\u00e4t n\u00e4hern und anfangen, die S\u00e4ule zu \u00fcberladen. Durch Verringerung des Injektionsvolumens auf den gew\u00fcnschten Bereich zwischen 1 und 5 Prozent k\u00f6nnen wir diese negative Auswirkung auf die Peakform wenigstens teilweise r\u00fcckg\u00e4ngig machen.<\/p>\n\n\n\n<p>Als n\u00e4chstes k\u00f6nnen wir versuchen, das L\u00f6semittel f\u00fcr unsere Probe zu \u00e4ndern, so dass es der anf\u00e4nglichen mobilen Phase entspricht. Dadurch werden Inkompatibilit\u00e4ten zwischen Probe und L\u00f6semittel vermieden, und wir k\u00f6nnen das Injektionsvolumen erh\u00f6hen, ohne Peakfronting zu beobachten, was ein Anzeichen von Proben\u00fcberladung ist.<\/p>\n\n\n\n<p>Das sind nur ein paar M\u00f6glichkeiten, die Sie in Ihrem Labor testen k\u00f6nnen, um die Peakform zu verbessern, ohne viele andere chromatografische Parameter zu \u00e4ndern. Nochmals vielen Dank f\u00fcr Ihr Interesse an unserem aktuellen Restek Tipp.<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>In diesem Restek-Tipp geht es um die Optimierung der Injektion f\u00fcr die Fl\u00fcssigchromatografie. Indem Sie das richtige Volumen eines kompatiblen L\u00f6semittels injizieren, erhalten Sie die beste Peakform!<\/p>\n","protected":false},"author":11,"featured_media":41175,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[772],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[2481],"resource-technique":[2326],"ppma_author":[414],"class_list":["post-42491","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-videos-de","product-library-flussigchromatographie-produkte","resource-technique-flussigchromatografie"],"acf":[],"taxonomy_info":{"category":[{"value":772,"label":"Videos"}],"product-library":[{"value":2481,"label":"Fl\u00fcssigchromatographie Produkte"}],"resource-technique":[{"value":2326,"label":"Fl\u00fcssigchromatografie"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-GNAV3621-1024x536.jpg",1024,536,true],"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/de\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":772,"name":"Videos","slug":"videos-de","term_group":0,"term_taxonomy_id":772,"taxonomy":"category","description":"","parent":0,"count":132,"filter":"raw","cat_ID":772,"category_count":132,"category_description":"","cat_name":"Videos","category_nicename":"videos-de","category_parent":0}],"tag_info":false,"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/42491","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/comments?post=42491"}],"version-history":[{"count":4,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/42491\/revisions"}],"predecessor-version":[{"id":85003,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/42491\/revisions\/85003"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/media\/41175"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/media?parent=42491"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/categories?post=42491"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/tags?post=42491"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/industries-application?post=42491"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/post-badge?post=42491"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-type?post=42491"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/product-library?post=42491"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-technique?post=42491"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/ppma_author?post=42491"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}