{"id":43358,"date":"2024-02-01T00:00:00","date_gmt":"2024-02-01T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/fast-analysis-of-delta-8-thc-delta-9-thc-and-isomeric-hydroxy-and-carboxy-metabolites-in-whole-blood-by-lc-msms\/"},"modified":"2026-01-28T15:52:45","modified_gmt":"2026-01-28T15:52:45","slug":"fast-analysis-of-delta-8-thc-delta-9-thc-and-isomeric-hydroxy-and-carboxy-metabolites-in-whole-blood-by-lc-msms","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/de\/application-notes-zh\/cfan4213\/fast-analysis-of-delta-8-thc-delta-9-thc-and-isomeric-hydroxy-and-carboxy-metabolites-in-whole-blood-by-lc-msms","title":{"rendered":"Fast Analysis of \u03948-THC, \u03949-THC, and Isomeric Hydroxy and Carboxy Metabolites in Whole Blood by LC-MS\/MS"},"content":{"rendered":"\n

Introduction<\/h2>\n\n\n\n

The testing of whole blood samples for tetrahydrocannabinol (\u03949-THC) consumption is routine and has been performed for many decades. Since \u03949-THC is metabolized into 11-hydroxy-\u03949-tetrahydrocannabinol (11-OH-\u03949-THC) and further into 11-nor-9-carboxy-\u03949-THC (\u03949-THC-COOH), it is important to test for the parent compound and both metabolites to properly monitor for THC usage. <\/p>\n\n\n\n

As more isomers of \u03949-THC become available on the market, testing has become more complicated, and novel methods are needed to achieve isomeric resolution. One such isomer, \u03948-THC, is federally unregulated in the United States and readily available for purchase in many stores. This compound forms its own hydroxylated and carboxylated metabolites, (11-OH-\u03948-THC and \u03948-THC-COOH), that must be resolved from their isomeric \u03949 counterparts. Chromatographic resolution of all three isomeric pairs is essential for reporting accurate clinical specimen results, and poor resolution, especially when one isomer is present in much greater abundance than the other, can cause invalid data. <\/p>\n\n\n\n

To improve reporting accuracy, the following method was developed to adequately resolve all six isomers in whole blood. Linearity, accuracy, precision, and the potential for cross-analyte interferences were assessed.<\/p>\n\n\n\n

Experimental<\/h2>\n\n\n\n

Liquid-Liquid Extraction [1]<\/em><\/h3>\n\n\n\n
    \n
  1. 500 \u00b5L aliquots of blank whole blood were added to glass test tubes.<\/li>\n\n\n\n
  2. 50 \u00b5L of calibrator or QC standard was added to each tube. 50 \u00b5L of internal standard (1000 ng\/mL of \u03949-THC-D3; 11-OH-\u03949-THC-D3; and \u03949-THC-COOH-D3) was added to each tube and vortexed.<\/li>\n\n\n\n
  3. 500 \u00b5L of HPLC grade water was added to each tube and vortexed.<\/li>\n\n\n\n
  4. 100 \u00b5L of 1N HCl was added to each tube and vortexed.<\/li>\n\n\n\n
  5. 2.5 mL of 80:20 hexanes:ethyl acetate was added to each tube, capped, and vortexed until visibly combined.<\/li>\n\n\n\n
  6. Samples were centrifuged at 4200 rpm for 15 minutes or until the two layers had completely separated.<\/li>\n\n\n\n
  7. The supernatant was pipetted off each sample, transferred to a clean test tube, and dried down under nitrogen.<\/li>\n\n\n\n
  8. Samples were reconstituted in 100 \u00b5L of 50:50 water:methanol (both containing 0.1% formic acid), vortexed, and transferred to an LC vial with an insert.<\/li>\n<\/ol>\n\n\n\n

    Instrument Parameters<\/em><\/h3>\n\n\n\n

    The LC-MS\/MS method detailed in Tables I and II was developed on a Raptor FluoroPhenyl column in order to optimally separate \u03948-THC; \u03949-THC; 11-OH-\u03948-THC; 11-OH-\u03949-THC; \u03948-THC-COOH; and \u03949-THC-COOH. MRM transitions and ESI mode for each analyte are given in Figure 1.<\/p>\n\n\n\n

    Table I:<\/strong> Chromatography Gradient<\/p>\n\n\n\n

    Time (min)<\/strong><\/td>Flow Rate (mL\/min)<\/strong><\/td>%A<\/strong><\/td>%B<\/strong><\/td><\/tr>
    0.00<\/td>0.8<\/td>36<\/td>64<\/td><\/tr>
    6.50<\/td>0.8<\/td>36<\/td>64<\/td><\/tr>
    6.60<\/td>0.8<\/td>32<\/td>68<\/td><\/tr>
    13.00<\/td>0.8<\/td>32<\/td>68<\/td><\/tr>
    13.10<\/td>0.8<\/td>0<\/td>100<\/td><\/tr>
    14.00<\/td>0.8<\/td>0<\/td>100<\/td><\/tr>
    14.10<\/td>0.8<\/td>36<\/td>64<\/td><\/tr>
    16.00<\/td>0.8<\/td>36<\/td>64<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

    Table II:<\/strong> LC Method Parameters<\/p>\n\n\n\n

    Column<\/strong><\/td>Raptor FluoroPhenyl, 2.7 \u03bcm, 100 mm x 3.0 mm ID (cat.# 9319A1E)<\/td><\/tr>
    Guard<\/strong><\/td>Raptor FluoroPhenyl EXP guard column cartridge 5 mm, 3.0 mm ID, 2.7 \u03bcm (cat.# 9319A0253)<\/td><\/tr>
    Column Temperature<\/strong><\/td>40 \u00b0C<\/td><\/tr>
    Mobile Phase A<\/strong><\/td>0.1% formic acid in water<\/td><\/tr>
    Mobile Phase B<\/strong><\/td>0.1% formic acid in methanol<\/td><\/tr>
    Injection Volume<\/strong><\/td>10 \u00b5L<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

    Results & Discussion<\/h2>\n\n\n\n

    Chromatographic Performance<\/em><\/h3>\n\n\n\n

    As shown in Figure 1, all three sets of isomers (six total analytes) were well separated in a fast 13-minute gradient (16-minute total analysis time) on a Raptor FluoroPhenyl column. The FluoroPhenyl stationary phase provided better selectivity for all three isomer pairs compared to alternative column chemistries, such as biphenyl or C18.<\/p>\n\n\n

    Figure 1:<\/strong> Separation of \u03948\/9-THC, Hydroxy, and Carboxy Metabolites in Whole Blood<\/div>
    \n
    \"\u03949-THC;<\/div>

    LC_CF0807<\/p>

    Peaks<\/h4>\n
    <\/th>Peaks<\/th>tR<\/sub> (min)<\/th>Conc.
    (ng\/mL)<\/th>    		Precursor<\/th>Product 1<\/th>Product 2<\/th>Mode<\/th><\/tr><\/thead>\n
    1.<\/td>11-OH-\u03948-THC<\/a><\/td>5.10<\/td>10<\/td>331.4<\/td>313.0<\/td>193.2<\/td>+<\/td><\/tr>\n
    2.<\/td>11-OH-\u03949-THC<\/a><\/td>5.49<\/td>10<\/td>331.4<\/td>313.0<\/td>193.2<\/td>+<\/td><\/tr>\n
    3.<\/td>\u03948-THC-COOH<\/td>5.54<\/td>50<\/td>343.0<\/td>298.9<\/td>244.8<\/td>–<\/td><\/tr>\n
    4.<\/td>\u03949-THC-COOH<\/td>6.45<\/td>50<\/td>343.0<\/td>298.9<\/td>244.8<\/td>–<\/td><\/tr>\n
    5.<\/td>\u03948-THC<\/a><\/td>11.67<\/td>10<\/td>315.5<\/td>193.0<\/td>123.0<\/td>+<\/td><\/tr>\n
    6.<\/td>\u03949-THC<\/a><\/td>12.18<\/td>10<\/td>315.5<\/td>193.0<\/td>123.0<\/td>+<\/td><\/tr>\n<\/tbody><\/table><\/div>

    Conditions<\/h4>
    Column<\/th>Raptor FluoroPhenyl (cat.# 9319A1E<\/a>)<\/td><\/tr>
    Dimensions:<\/th>100 mm x 3.0 mm ID<\/td><\/tr>
    Particle Size:<\/th>2.7 \u00b5m<\/td><\/tr>
    Pore Size:<\/th>90 \u00c5<\/td><\/tr>
    <\/td>
    Guard Column:<\/th>Raptor FluoroPhenyl EXP guard column cartridge 5 mm, 3.0 mm ID, 2.7 \u00b5m (cat.# 9319A0253<\/a>)<\/td><\/tr>
    Temp.:<\/th>40 \u00b0C<\/td><\/tr>
    Standard\/Sample<\/th><\/td><\/tr>
    <\/td>\u03948-Tetrahydrocannabinol (\u03948-THC) (cat.# 34090<\/a>)<\/td><\/tr>
    <\/td>\u03949-Tetrahydrocannabinol (\u03949-THC) (cat.# 34067<\/a>)<\/td><\/tr>
    <\/td>(\u00b1)11-nor-9-carboxy-\u0394-9-THC (\u03949-THC-COOH) (cat.# 34068<\/a>)<\/td><\/tr>
    <\/td>Other compounds obtained separately. <\/td><\/tr>\n
    Diluent:<\/th>50:50 Methanol:water, both with 0.1% formic acid<\/td><\/tr><\/td><\/tr>
    Inj. Vol.:<\/th>10 \u00b5L <\/td><\/tr>
    Mobile Phase<\/th><\/td><\/tr>
    A:<\/th>Water, 0.1% formic acid <\/td><\/tr>
    B:<\/th>Methanol, 0.1% formic acid <\/td><\/tr>
    <\/td>
    Time (min)<\/th>Flow (mL\/min)<\/th>%A<\/th>%B<\/th><\/tr><\/thead>
    0.00<\/td>0.8<\/td>36<\/td>64<\/td><\/tr>
    6.50<\/td>0.8<\/td>36<\/td>64<\/td><\/tr>
    6.60<\/td>0.8<\/td>32<\/td>68<\/td><\/tr>
    13.00<\/td>0.8<\/td>32<\/td>68<\/td><\/tr>
    13.10<\/td>0.8<\/td>0<\/td>100<\/td><\/tr>
    14.00<\/td>0.8<\/td>0<\/td>100<\/td><\/tr>
    14.10<\/td>0.8<\/td>36<\/td>64<\/td><\/tr>
    16.00<\/td>0.8<\/td>36<\/td>64<\/td><\/tr><\/tbody><\/table><\/td><\/tr>
    Max Pressure:<\/th>440 bar<\/td><\/tr><\/table>
    Detector<\/th>SCIEX 4500 MS\/MS<\/td><\/tr>
    Ion Source:<\/th>Electrospray <\/td><\/tr>
    Ion Mode:<\/th>ESI+\/ESI- <\/td><\/tr>
    Sample Preparation<\/th>500 \u00b5L of whole blood was transferred to a 15 mL glass test tube. 50 \u00b5L of internal standard and 50 \u00b5L of control material were transferred to the test tube and vortexed. 500 \u00b5L of HPLC grade water was added to each sample and vortexed. 100 \u00b5L of 1N HCl was added to each sample and vortexed. 2.5 mL of 80:20 hexanes:ethyl acetate was added to each sample and vortexed until visibly combined. Samples were centrifuged at 4200 rpm for 15 minutes. The top layer was transferred to a new glass test tube and dried down under nitrogen. Samples were reconstituted with 100 \u00b5L of 50:50 methanol:water, both containing 0.1% formic acid, and vortexed. Samples were transferred to 2 mL screw-thread vials (cat.# 21143<\/a>) with glass inserts (cat.# 21776<\/a>) and capped with short-cap, screw-vial closures (cat.# 24498<\/a>).<\/td><\/tr><\/table><\/div><\/div><\/div>
    <\/path><\/g><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n
    <\/div>\n\n\n\n

    Linearity, Accuracy, and Precision<\/em><\/h3>\n\n\n\n

    Linearity was demonstrated using a 1\/x2<\/sup> weighted linear regression, and all analytes showed acceptable R2<\/sup> values (\u22650.99). The linear calibration ranges were 0.5\u2013100 ng\/mL for 11-OH-\u03948-THC; 11-OH-\u03949-THC; \u03948-THC; and \u03949-THC and 2.5\u2013500 ng\/mL for \u03948-THC-COOH and \u03949-THC-COOH.<\/p>\n\n\n\n

    Accuracy and precision were assessed on three different days using low, mid, and high QC samples spiked at 5, 10, and 50 ng\/mL for 11-OH-\u03948-THC; 11-OH-\u03949-THC; \u03948-THC; and \u03949-THC and at 25, 50, and 250 ng\/mL for \u03948-THC-COOH and \u03949-THC-COOH. Method accuracy was demonstrated by recovery values being within 10% of the nominal concentrations for the QC samples at all levels. The %RSD was under 20% for both intraday and interday testing, indicating acceptable method precision.<\/p>\n\n\n\n

    Cross-Analyte Interferences<\/em><\/h3>\n\n\n\n

    To demonstrate that other naturally occurring cannabinoids would not interfere with the target compounds, we spiked whole blood with nine commonly encountered and\/or structurally similar cannabinoids: CBDV, CBD, CBG, THCV, exo-THC, CBL, CBN, \u039410-THC, and CBC. All nine cannabinoids were fully resolved from the analytes of interest on the Raptor FluoroPhenyl column and no cross-analyte interferences are expected (Figure 2).<\/p>\n\n\n

    Figure 2:<\/strong> Separation of \u03948\/9-THC, Hydroxy, and Carboxy Metabolites from Nine Cannabinoids\u00a0<\/div>
    \n
    \"\u03949-THC;<\/div>

    LC_CF0808<\/p>

    Peaks<\/h4>\n
    <\/th>Peaks<\/th>tR<\/sub> (min)<\/th>Conc.
    (ng\/mL)<\/th>    		Precursor<\/th>Product 1<\/th>Product 2<\/th>Mode<\/th><\/tr><\/thead>\n
    1.<\/td>CBDV<\/a><\/td>3.52<\/td>50<\/td>287.4<\/td>165.0<\/td>231.1<\/td>+<\/td><\/tr>\n
    2.<\/td>11-OH-\u03948-THC<\/a><\/td>5.09<\/td>50<\/td>331.4<\/td>313.0<\/td>193.2<\/td>+<\/td><\/tr>\n
    3.<\/td>11-OH-\u03949-THC<\/a><\/td>5.50<\/td>50<\/td>331.4<\/td>313.0<\/td>193.2<\/td>+<\/td><\/tr>\n
    4.<\/td>\u03948-THC-COOH<\/td>5.53<\/td>250<\/td>343.0<\/td>298.9<\/td>244.8<\/td>–<\/td><\/tr>\n
    5.<\/td>\u03949-THC-COOH<\/td>6.44<\/td>250<\/td>343.0<\/td>298.9<\/td>244.8<\/td>–<\/td><\/tr>\n
    6.<\/td>CBD<\/a><\/td>6.81<\/td>50<\/td>315.5<\/td>193.0<\/td>259.0<\/td>+<\/td><\/tr>\n
    7.<\/td>CBG<\/a><\/td>7.56<\/td>50<\/td>317.5<\/td>193.0<\/td>123.0<\/td>+<\/td><\/tr>\n
    8.<\/td>THCV<\/a><\/td>7.65<\/td>50<\/td>287.4<\/td>165.0<\/td>123.0<\/td>+<\/td><\/tr>\n
    9.<\/td>exo-THC<\/a><\/td>10.63<\/td>50<\/td>315.5<\/td>193.0<\/td>259.0<\/td>+<\/td><\/tr>\n
    10.<\/td>\u03948-THC<\/a><\/td>11.67<\/td>50<\/td>315.5<\/td>193.0<\/td>123.0<\/td>+<\/td><\/tr>\n
    11.<\/td>\u03949-THC<\/a><\/td>12.17<\/td>50<\/td>315.5<\/td>193.0<\/td>123.0<\/td>+<\/td><\/tr>\n
    12.<\/td>CBL<\/a><\/td>12.43<\/td>50<\/td>315.5<\/td>235.2<\/td>193.0<\/td>+<\/td><\/tr>\n
    13.<\/td>\u039410-THC<\/td>13.88<\/td>50<\/td>315.5<\/td>193.1<\/td>259.0<\/td>+<\/td><\/tr>\n
    14.<\/td>CBN<\/a><\/td>13.93<\/td>50<\/td>311.4<\/td>223.0<\/td>293.1<\/td>+<\/td><\/tr>\n
    15.<\/td>CBC<\/a><\/td>13.93<\/td>50<\/td>315.5<\/td>193.1<\/td>259.0<\/td>+<\/td><\/tr>\n<\/tbody><\/table><\/div>

    Conditions<\/h4>
    Column<\/th>Raptor FluoroPhenyl (cat.# 9319A1E<\/a>)<\/td><\/tr>
    Dimensions:<\/th>100 mm x 3.0 mm ID<\/td><\/tr>
    Particle Size:<\/th>2.7 \u00b5m<\/td><\/tr>
    Pore Size:<\/th>90 \u00c5<\/td><\/tr>
    <\/td>
    Guard Column:<\/th>Raptor FluoroPhenyl EXP guard column cartridge 5 mm, 3.0 mm ID, 2.7 \u00b5m (cat.# 9319A0253<\/a>)<\/td><\/tr>
    Temp.:<\/th>40 \u00b0C<\/td><\/tr>
    Standard\/Sample<\/th><\/td><\/tr>
    <\/td>Cannabinoids Neutrals 9 standard (cat.# 34132<\/a>)<\/td><\/tr>
    <\/td>\u03948-Tetrahydrocannabinol (\u03948-THC) (cat.# 34090<\/a>)<\/td><\/tr>
    <\/td>\u03949-Tetrahydrocannabinol (\u03949-THC) (cat.# 34067<\/a>)<\/td><\/tr>
    <\/td>(\u00b1)11-nor-9-carboxy-\u0394-9-THC (\u03949-THC-COOH) (cat.# 34068<\/a>)<\/td><\/tr>
    <\/td>Other compounds obtained separately. <\/td><\/tr>\n
    Diluent:<\/th>50:50 Methanol:water, both with 0.1% formic acid<\/td><\/tr><\/td><\/tr>
    Inj. Vol.:<\/th>10 \u00b5L <\/td><\/tr>
    Mobile Phase<\/th><\/td><\/tr>
    A:<\/th>Water, 0.1% formic acid <\/td><\/tr>
    B:<\/th>Methanol, 0.1% formic acid <\/td><\/tr>
    <\/td>
    Time (min)<\/th>Flow (mL\/min)<\/th>%A<\/th>%B<\/th><\/tr><\/thead>
    0.00<\/td>0.8<\/td>36<\/td>64<\/td><\/tr>
    6.50<\/td>0.8<\/td>36<\/td>64<\/td><\/tr>
    6.60<\/td>0.8<\/td>32<\/td>68<\/td><\/tr>
    13.00<\/td>0.8<\/td>32<\/td>68<\/td><\/tr>
    13.10<\/td>0.8<\/td>0<\/td>100<\/td><\/tr>
    14.00<\/td>0.8<\/td>0<\/td>100<\/td><\/tr>
    14.10<\/td>0.8<\/td>36<\/td>64<\/td><\/tr>
    16.00<\/td>0.8<\/td>36<\/td>64<\/td><\/tr><\/tbody><\/table><\/td><\/tr>
    Max Pressure:<\/th>440 bar<\/td><\/tr><\/table>
    Detector<\/th>SCIEX 4500<\/td><\/tr>
    Ion Source:<\/th>Electrospray <\/td><\/tr>
    Ion Mode:<\/th>ESI+\/ESI- <\/td><\/tr>
    Sample Preparation<\/th>500 \u00b5L of whole blood was transferred to a 15 mL glass test tube. 50 \u00b5L of internal standard and 50 \u00b5L of control material were transferred to the test tube and vortexed. 500 \u00b5L of HPLC grade water was added to each sample and vortexed. 100 \u00b5L of 1N HCl was added to each sample and vortexed. 2.5 mL of 80:20 hexanes:ethyl acetate was added to each sample and vortexed until visibly combined. Samples were centrifuged at 4200 rpm for 15 minutes. The top layer was transferred to a new glass test tube and dried down under nitrogen. Samples were reconstituted with 100 \u00b5L of 50:50 methanol:water, both containing 0.1% formic acid, and vortexed. Samples were transferred to 2 mL screw-thread vials (cat.# 21143<\/a>) with glass inserts (cat.# 21776<\/a>) and capped with short-cap, screw-vial closures (cat.# 24498<\/a>).<\/td><\/tr><\/table><\/div><\/div><\/div>
    <\/path><\/g><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n
    <\/div>\n\n\n\n

    Conclusion<\/h2>\n\n\n\n

    The fast LC-MS\/MS method established here on a Raptor FluoroPhenyl column provides essential chromatographic resolution of \u03948-THC and \u03949-THC isomers as well as their isomeric hydroxy and carboxy metabolites in whole blood. In addition, the target compounds were completely separated from potentially interfering cannabinoids, helping ensure that labs can report more accurate clinical results.<\/p>\n\n\n\n

    References<\/h2>\n\n\n\n

    N.B. Tiscione, R . Miller, X . Shan, J . Sprague, D.T. Yeatman, An efficient, robust method for the determination of cannabinoids in whole blood by LC-MS-MS, J. Ana.l Toxicol. 40(8) (2016) 639-648. https:\/\/doi.org\/10.1093\/jat\/bkw063<\/a><\/p>\n\n\n\n

    <\/div>\n\n\n
    \n

    Products Mentioned<\/h3>\n
    \n
    \n
    \n
    \n Catalog No. 9319A1E <\/a> <\/div>\n
    \n
    Raptor FluoroPhenyl, 2.7 \u00b5m, 100×3.0 mm HPLC-S\u00e4ule<\/div>\n <\/div>\n
    \n Produkt anzeigen<\/a>\n <\/div>\n <\/div>\n
    \n
    \n Catalog No. 9319A0253 <\/a> <\/div>\n
    \n
    Raptor FluoroPhenyl EXP Vors\u00e4ulenkartusche, 2.7 \u00b5m, 5 x 3.0 mm, 3er Pack<\/div>\n <\/div>\n
    \n Produkt anzeigen<\/a>\n <\/div>\n <\/div>\n
    \n
    \n Catalog No. 34090 <\/a> <\/div>\n
    \n
    d8-Tetrahydrocannabinol (d8-THC) Standard, 1000 \u00b5g\/mL in P&T Methanol, 1 mL\/Ampulle<\/div>\n <\/div>\n
    \n Produkt anzeigen<\/a>\n <\/div>\n <\/div>\n
    \n
    \n Catalog No. 34067 <\/a> <\/div>\n
    \n
    d9-THC Standard, 1000 \u00b5g\/mL, P&T Methanol, 1 mL\/Ampulle<\/div>\n <\/div>\n
    \n Produkt anzeigen<\/a>\n <\/div>\n <\/div>\n
    \n
    \n Catalog No. 34068 <\/a> <\/div>\n
    \n
    (\u00b1)11-nor-9-carboxy-Delta-9-THC Standard, 100 \u00b5g\/mL, Methanol, 1 mL\/Ampulle<\/div>\n <\/div>\n
    \n Produkt anzeigen<\/a>\n <\/div>\n <\/div>\n
    \n
    \n Catalog No. 21143 <\/a> <\/div>\n
    \n
    Short-Cap-Vial mit graduiertem Markierungsfeld, 9-425 Screw-Thread, 2.0 mL, 9 mm, 12 x 32 (nur Vial), braun, 1000er Pack<\/div>\n <\/div>\n
    \n Produkt anzeigen<\/a>\n <\/div>\n <\/div>\n
    \n
    \n Catalog No. 21776 <\/a> <\/div>\n
    \n
    Vial-Eins\u00e4tze, Glas, Big Mouth mit Bodenfeder, 250 \u03bcL, 100er Pack<\/div>\n <\/div>\n
    \n Produkt anzeigen<\/a>\n <\/div>\n <\/div>\n
    \n
    \n Catalog No. 24498 <\/a> <\/div>\n
    \n
    Short Screw Kappen, Polypropylen, Screw-Thread, PTFE\/Silikon\/PTFE Septen, blau, vormontiert, 2.0 mL, 9 mm, 1000er Pack<\/div>\n <\/div>\n
    \n Produkt anzeigen<\/a>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n \n\n\n
    <\/div>\n","protected":false},"excerpt":{"rendered":"

    Separate \u03948-THC, \u03949-THC, and their isomeric hydroxy and carboxy metabolites quickly and completely with this LC-MS\/MS method for analyzing whole blood 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