{"id":43560,"date":"2021-01-12T13:30:00","date_gmt":"2021-01-12T13:30:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/schnelle-direktanalyse-underivatisierter-aminosauren-in-sauglingsanfangsnahrung\/"},"modified":"2026-01-28T22:32:20","modified_gmt":"2026-01-28T22:32:20","slug":"schnelle-direktanalyse-underivatisierter-aminosauren-in-sauglingsanfangsnahrung","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/de\/artikel\/fffa3436-de\/schnelle-direktanalyse-underivatisierter-aminosauren-in-sauglingsanfangsnahrung","title":{"rendered":"Schnelle Direktanalyse underivatisierter Aminos\u00e4uren in S\u00e4uglingsanfangsnahrung"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Einfache One-Step-Probenvorbereitung f\u00fcr die Direktanalyse underivatisierter Aminos\u00e4uren.<\/li>\n\n\n\n<li>Gleichzeitige Analyse nichtpolarer, polarer, positiv geladener und negativ geladener Aminos\u00e4uren in einem kurzen 10-min\u00fctigen Lauf.<\/li>\n\n\n\n<li>Sorgf\u00e4ltig entwickelter Gradient trennt Kontaminanten von den Zielanalyten.\u00a0<\/li>\n<\/ul>\n\n\n\n<p>Um sicherzustellen, dass der N\u00e4hrstoffbedarf erf\u00fcllt wird, werden zuverl\u00e4ssige Analysemethoden f\u00fcr die genaue Bestimmung freier Aminos\u00e4uren in S\u00e4uglingsanfangsnahrung ben\u00f6tigt. Ein typischer Ansatz ist eine Vors\u00e4ulenderivatisierung gefolgt von einer Reversed-Phase-LC-Analyse, wobei die Derivatisierung aufgrund der mangelnden Retention und schlechten Empfindlichkeit der freien Aminos\u00e4uren erforderlich ist. Diese Methoden sind jedoch oft zeitaufwendig und arbeitsintensiv. Die Verwendung von perfluorierten S\u00e4uren als Ionenpaarreagenzien zur Verbesserung der Retention von underivatisierten Aminos\u00e4uren auf einer C18-S\u00e4ule ist eine weitere h\u00e4ufig verwendete Methode; dieses Verfahren kann sich aber negativ auf das chromatographische System und das Massenspektrometer auswirken.&nbsp;<\/p>\n\n\n\n<p>Beim hier vorgestellten einfacheren Verfahren k\u00f6nnen underivatisierte Aminos\u00e4uren im Anschluss an eine One-Step-Probenvorbereitung direkt bestimmt werden, wenn eine Raptor Polar X-S\u00e4ule in Verbindung mit einem MS\/MS-Detektor verwendet wird. Raptor Polar X-S\u00e4ulen besitzen eine Hybrid-Phasenchemie (HILIC und Ionenaustausch), die das f\u00fcr die gleichzeitige Analyse einer Vielzahl von Analyten erforderliche ausgewogene Retentionsverhalten bereitstellt. Underivatisierte Aminos\u00e4uren mit nichtpolaren, polaren, positiv geladenen und negativ geladenen Seitenketten wurden ausreichend retardiert und dann mithilfe eines 10-min\u00fctigen Gradienten schnell eluiert. Dieser Gradient war au\u00dferdem in der Lage, Kontaminanten von den Zielanalyten zu trennen, was die Methode robuster macht. Zeitaufwendige und arbeitsintensive Verfahren zur Probenvorbereitung, die auch teure Derivatisierungskits einschlie\u00dfen k\u00f6nnen, lassen sich so durch eine einfache Proteinf\u00e4llung und Direktanalyse des resultierenden Extrakts ersetzen. Die Direktanalyse underivatisierter Aminos\u00e4uren auf einer Raptor Polar X-S\u00e4ule liefert ausgezeichnete Ergebnisse in einem schnellen einfachen Arbeitsablauf und ist damit eine vorteilhafte Alternative zu herk\u00f6mmlichen Methoden.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0579.png\" alt=\"Underivatized Amino Acids Analysis in Baby Formula on Raptor Polar X\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FF0579<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tryptophan\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/54-12-6\/Tryptophan\" target=\"_blank\" rel=\"noopener\">Tryptophan<\/a><\/td><td class=\"oth\">1.17<\/td><td class=\"oth\">205.07<\/td><td class=\"oth\">146.08<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Phenylalanine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/63-91-2\/Phenylalanine\" target=\"_blank\" rel=\"noopener\">Phenylalanine<\/a><\/td><td class=\"oth\">1.26<\/td><td class=\"oth\">166.13<\/td><td class=\"oth\">120.10<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Leucine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/61-90-5\/Leucine\" target=\"_blank\" rel=\"noopener\">Leucine<\/a><\/td><td class=\"oth\">1.41<\/td><td class=\"oth\">132.13<\/td><td class=\"oth\">86.10<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Isoleucine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/73-32-5\/Isoleucine\" target=\"_blank\" rel=\"noopener\">Isoleucine<\/a><\/td><td class=\"oth\">1.55<\/td><td class=\"oth\">132.13<\/td><td class=\"oth\">86.10<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Methionine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/59-51-8\/Methionine\" target=\"_blank\" rel=\"noopener\">Methionine<\/a><\/td><td class=\"oth\">1.62<\/td><td class=\"oth\">150.07<\/td><td class=\"oth\">104.10<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tyrosine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/556-03-6\/Tyrosine\" target=\"_blank\" rel=\"noopener\">Tyrosine<\/a><\/td><td class=\"oth\">1.69<\/td><td class=\"oth\">182.10<\/td><td class=\"oth\">136.08<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Taurine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/107-35-7\/Taurine\" target=\"_blank\" rel=\"noopener\">Taurine<\/a><\/td><td class=\"oth\">1.91<\/td><td class=\"oth\">126.07<\/td><td class=\"oth\">108.07<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Valine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/516-06-3\/Valine\" target=\"_blank\" rel=\"noopener\">Valine<\/a><\/td><td class=\"oth\">1.98<\/td><td class=\"oth\">118.13<\/td><td class=\"oth\">72.11<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Proline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/609-36-9\/Proline\" target=\"_blank\" rel=\"noopener\">Proline<\/a><\/td><td class=\"oth\">2.29<\/td><td class=\"oth\">116.13<\/td><td class=\"oth\">70.09<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Alanine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/302-72-7\/Alanine\" target=\"_blank\" rel=\"noopener\">Alanine<\/a><\/td><td class=\"oth\">3.00<\/td><td class=\"oth\">90.03<\/td><td class=\"oth\">44.10<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Threonine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/80-68-2\/Threonine\" target=\"_blank\" rel=\"noopener\">Threonine<\/a><\/td><td class=\"oth\">3.12<\/td><td class=\"oth\">120.13<\/td><td class=\"oth\">74.08<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glycine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56-40-6\/Glycine\" target=\"_blank\" rel=\"noopener\">Glycine<\/a><\/td><td class=\"oth\">3.62<\/td><td class=\"oth\">76.10<\/td><td class=\"oth\">30.11<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glutamine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56-85-9\/Glutamine\" target=\"_blank\" rel=\"noopener\">Glutamine<\/a><\/td><td class=\"oth\">3.87<\/td><td class=\"oth\">147.13<\/td><td class=\"oth\">84.07<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Serine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/302-84-1\/Serine\" target=\"_blank\" rel=\"noopener\">Serine<\/a><\/td><td class=\"oth\">3.93<\/td><td class=\"oth\">106.07<\/td><td class=\"oth\">60.09<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Asparagine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/70-47-3\/Asparagine\" target=\"_blank\" rel=\"noopener\">Asparagine<\/a><\/td><td class=\"oth\">4.08<\/td><td class=\"oth\">133.13<\/td><td class=\"oth\">74.07<\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Arginine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/74-79-3\/Arginine\" target=\"_blank\" rel=\"noopener\">Arginine<\/a><\/td><td class=\"oth\">4.47<\/td><td class=\"oth\">175.17<\/td><td class=\"oth\">70.09<\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Histidine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/71-00-1\/Histidine\" target=\"_blank\" rel=\"noopener\">Histidine<\/a><\/td><td class=\"oth\">4.66<\/td><td class=\"oth\">156.07<\/td><td class=\"oth\">110.16<\/td><\/tr>\n<tr><td class=\"num\">18.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Lysine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56-87-1\/Lysine\" target=\"_blank\" rel=\"noopener\">Lysine<\/a><\/td><td class=\"oth\">4.97<\/td><td class=\"oth\">147.13<\/td><td class=\"oth\">84.13<\/td><\/tr>\n<tr><td class=\"num\">19.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glutamic acid\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/617-65-2\/Glutamic acid\" target=\"_blank\" rel=\"noopener\">Glutamic acid<\/a><\/td><td class=\"oth\">5.89<\/td><td class=\"oth\">148.10<\/td><td class=\"oth\">84.10<\/td><\/tr>\n<tr><td class=\"num\">20.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cystine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56-89-3\/Cystine\" target=\"_blank\" rel=\"noopener\">Cystine<\/a><\/td><td class=\"oth\">6.10<\/td><td class=\"oth\">241.13<\/td><td class=\"oth\">152.00<\/td><\/tr>\n<tr><td class=\"num\">21.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Aspartic acid\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/617-45-8\/Aspartic acid\" target=\"_blank\" rel=\"noopener\">Aspartic acid<\/a><\/td><td class=\"oth\">7.12<\/td><td class=\"oth\">134.07<\/td><td class=\"oth\">74.06<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FF0579\" rel=\"noopener\">cat.# 9311A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>20:80 Water:acetonitrile, 0.01 N HCl<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>  Endogenous amino acids<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 0.5% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Mobile Phase B: 9:1 Acetonitrile:20 mM ammonium formate in water (pH 3.0) (The ammonium formate concentration is 20 mM relative to the total volume of mobile phase B. See preparation notes for instructions on diluting a 200 mM aqueous starting solution.) <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>12<\/td><td>88<\/td><\/tr><tr><td>3.50<\/td><td>0.5<\/td><td>12<\/td><td>88<\/td><\/tr><tr><td>8.00<\/td><td>0.5<\/td><td>70<\/td><td>30<\/td><\/tr><tr><td>8.01<\/td><td>0.5<\/td><td>12<\/td><td>88<\/td><\/tr><tr><td>10.0<\/td><td>0.5<\/td><td>12<\/td><td>88<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>A 200 \u00b5L aliquot of protein hydrolysate formula (Similac ALIMENTUM) was mixed with 800 \u00b5L of acetonitrile and 10 \u00b5L of 1 N HCl. After centrifugation at 4000 rpm for 5 minutes, the supernatant was diluted 20-fold with 20:80 water:acetonitrile (0.01 N HCl) and injected for analysis.<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td><i>Mobile Phase B Preparation:<\/i> To make 500 mL of mobile phase B, measure ~45 mL of water into a small beaker and add 1 mL of 10 M ammonium formate solution. Adjust pH to 3.0 by adding formic acid and then bring the volume to 50 mL with water. Combine this 50 mL ammonium formate solution (pH 3.0) with 450 mL of acetonitrile to complete the preparation.   <\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0579.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Diese direkte LC-MS\/MS-Analyse nicht derivatisierter Aminos\u00e4uren in S\u00e4uglingsnahrung gew\u00e4hrtleistet einen einfachen Workflow, der eine gute Retention und schnelle Elution einer breiten Palette von Aminos\u00e4uren erm\u00f6glicht.<\/p>\n","protected":false},"author":11,"featured_media":90021,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[787],"tags":[],"industries-application":[2236,2237],"post-badge":[],"resource-type":[],"product-library":[2481,2499],"resource-technique":[2326,2363],"ppma_author":[414],"class_list":["post-43560","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-artikel","industries-application-lebensmittel-getranke","industries-application-lebensmittelqualitat","product-library-flussigchromatographie-produkte","product-library-lc-saulen","resource-technique-flussigchromatografie","resource-technique-ms-ms-de"],"acf":[],"taxonomy_info":{"category":[{"value":787,"label":"Artikel"}],"industries-application":[{"value":2236,"label":"Lebensmittel &amp; 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