{"id":43604,"date":"2021-01-12T00:00:00","date_gmt":"2021-01-12T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/schnelle-und-robuste-direktanalyse-von-polaren-pestiziden-in-spinat\/"},"modified":"2025-12-16T20:16:10","modified_gmt":"2025-12-16T20:16:10","slug":"schnelle-und-robuste-direktanalyse-von-polaren-pestiziden-in-spinat","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/de\/artikel\/fsfa3215-de\/schnelle-und-robuste-direktanalyse-von-polaren-pestiziden-in-spinat","title":{"rendered":"Schnelle und robuste Direktanalyse von polaren Pestiziden in Spinat"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Keine zeitaufwendige Derivatisierung, Ionenpaarreagenzien oder lange \u00c4quilibrierungszeiten.<\/li>\n\n\n\n<li>Spezielle station\u00e4re Phase bietet gute Retention und effiziente Elution mit besserer Peakform.<\/li>\n\n\n\n<li>Robuste S\u00e4ulen-Performance garantiert konsistente Chromatographie und zuverl\u00e4ssige Ergebnisse.<\/li>\n<\/ul>\n\n\n\n<p>Die Direktanalyse von polaren Pestiziden wie Glyphosat, seines Prim\u00e4rmetaboliten AMPA und Glufosinat ist schwierig, da diese auf Reversed-Phase-S\u00e4ulen nicht gut retardiert werden und aufgrund von Chelatbildung mit Metalloberfl\u00e4chen im Probenflussweg des LC-MS\/MS-Systems oft eine schlechte Response zeigen. Derivatisierung und Ionenpaarreagenzien werden h\u00e4ufig zur Verbesserung der Performance eingesetzt, erfordern jedoch zus\u00e4tzliche Zeit zur Probenvorbereitung und k\u00f6nnen unerw\u00fcnschte Nebenwirkungen produzieren (Ausf\u00e4llungen, lange \u00c4quilibrierungszeiten, Systemkontamination usw.).&nbsp;<\/p>\n\n\n\n<p>Eine bessere L\u00f6sung ist die Verwendung einer Raptor Polar X S\u00e4ule, deren neuartige station\u00e4re Phase eine st\u00e4rkere Wechselwirkung mit polaren anionischen Verbindungen erm\u00f6glicht. Sie nutzt spezielle Hybrid-Retentionsmodi (HILIC und Ionenaustausch) und bietet hohe Retention, erlaubt aber dennoch die rasche Elution der Zielanalyten als symmetrische Peaks durch einfachen Wechsel der mobilen Phase. In der vorliegenden Analyse von polaren Pestiziden in Spinat l\u00e4sst sich f\u00fcr alle drei Komponenten eine ausgezeichnete chromatographische Performance bei einer Gesamtanalysezeit von nur 8 Minuten beobachten. Dar\u00fcber hinaus bleiben die Peakformen und Retentionszeiten \u00fcber Hunderte von Matrixinjektionen stabil und die volle Leistungsf\u00e4higkeit wird durch einen einfachen Wechsel der Vors\u00e4ule wiederhergestellt. Der schnelle, einfache und effektive Workflow ist eine gute Alternative f\u00fcr Lebensmittellabore, die die Direktanalyse von polaren Pestiziden ohne umst\u00e4ndliche und zeitaufwendige Verfahren durchf\u00fchren m\u00f6chten.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0547.png\" alt=\"Polar Pesticides in Spinach on Raptor Polar X by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0547<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><th style=\"text-align: center;width: 75px\">Product Ion 3<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">AMPA<\/td><td class=\"oth\">100<\/td><td class=\"oth\">110.10<\/td><td class=\"oth\">79.05<\/td><td class=\"oth\">63.10<\/td><td class=\"oth\">81.10<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glufosinate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/51276-47-2\/Glufosinate\" target=\"_blank\" rel=\"noopener\">Glufosinate<\/a><\/td><td class=\"oth\">100<\/td><td class=\"oth\">180.20<\/td><td class=\"oth\">85.15<\/td><td class=\"oth\">95.10<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glyphosate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1071-83-6\/Glyphosate\" target=\"_blank\" rel=\"noopener\">Glyphosate<\/a><\/td><td class=\"oth\">100<\/td><td class=\"oth\">168.10<\/td><td class=\"oth\">63.05<\/td><td class=\"oth\">79.05<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a class=\"ga-cgram-prod-link\" target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A32\" rel=\"noopener\">cat.# 9311A32<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>30 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Raptor Polar X guard column cartridge 5 mm, 2.1 mm ID, 2.7 \u00b5m (<a class=\"ga-cgram-prod-link\" target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A0252\" rel=\"noopener\">cat.# 9311A0252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Spinach extract<\/td><\/tr>\n<td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water,  0.5% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile, 0.5% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>5.0<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>6.5<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>6.51<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>8<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>Frozen spinach was added to a Blixer processor with dry ice (3:1-4:1 ratio) and then ground into a very fine powder. The homogenate was placed into the freezer immediately. A 5.0 gram sample of the spinach powder was weighed into a 50 mL centrifuge tube (<a class=\"ga-cgram-prod-link\" target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/25846\" rel=\"noopener\">cat.# 25846<\/a>). According to the QuPPe method (Quick Polar Pesticides Method), 5.0 mL of methanol with 1.0% formic acid was added into the centrifuge tube. The tube was shaken by hand for 1 min and then by a mechanical shaker vigorously for 5 min. After centrifuging for 10 min at 4200 rpm, the supernatant was filtered through a 0.22 \u03bcm filter (<a class=\"ga-cgram-prod-link\" target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/23984\" rel=\"noopener\">cat.# 23984<\/a>). The final extract was fortified with the AMPA, glufosinate, and glyphosate at a final concentration of 100 ng\/mL.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-related-skus\"><h4>Related SKUs<\/h4><ul><li>9311A0252<\/li><li>9311A32<\/li><li>25846<\/li><li>23984<\/li><\/ul><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0547.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Dieser schnelle, einfache LC-MS\/MS-Arbeitsablauf f\u00fcr die direkte Analyse von polaren Pestiziden in Spinat bietet eine gute Retention und Peakform \u00fcber Hunderte von Matrixinjektionen auf einer Raptor Polar X-S\u00e4ule.<\/p>\n","protected":false},"author":11,"featured_media":70563,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[787],"tags":[],"industries-application":[2236,2238],"post-badge":[],"resource-type":[],"product-library":[2481,2499],"resource-technique":[2326,2363],"ppma_author":[414],"class_list":["post-43604","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-artikel","industries-application-lebensmittel-getranke","industries-application-lebensmittelsicherheit","product-library-flussigchromatographie-produkte","product-library-lc-saulen","resource-technique-flussigchromatografie","resource-technique-ms-ms-de"],"acf":[],"taxonomy_info":{"category":[{"value":787,"label":"Artikel"}],"industries-application":[{"value":2236,"label":"Lebensmittel &amp; 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