{"id":43615,"date":"2022-08-17T00:00:00","date_gmt":"2022-08-17T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/multi-mykotoxin-methode-fur-alternaria-toxine-epimere-der-ergotalkaloiden-und-andere-weitverbreitete-mykotoxine-in-lebensmitteln\/"},"modified":"2025-12-23T14:28:17","modified_gmt":"2025-12-23T14:28:17","slug":"multi-mykotoxin-methode-fur-alternaria-toxine-epimere-der-ergotalkaloiden-und-andere-weitverbreitete-mykotoxine-in-lebensmitteln","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/de\/artikel\/fsfa3904-de\/multi-mykotoxin-methode-fur-alternaria-toxine-epimere-der-ergotalkaloiden-und-andere-weitverbreitete-mykotoxine-in-lebensmitteln","title":{"rendered":"Multi-Mykotoxin-Methode f\u00fcr Alternaria-Toxine, Epimere der Ergotalkaloiden und andere weitverbreitete Mykotoxine in Lebensmitteln"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Werden Sie produktiver &#8211; Integrieren Sie neue Mykotoxine in Ihre bestehende Mykotoxine Methode!<\/li>\n\n\n\n<li>Sparen Sie sich zus\u00e4tzliche L\u00e4ufe bei hohen pH-Werten &#8211; die Raptor Biphenyl analysiert alle Mykotoxin-Gruppen quantitativ unter MS-kompatiblen sauren&nbsp;Bedingungen.<\/li>\n\n\n\n<li>Die einfache, universelle Probenvorbereitung spart Zeit und erfordert keine weiteren Reinigungsschritte.<\/li>\n<\/ul>\n\n\n\n<p>Die zunehmende gesetzliche Regulierung der Mykotoxine erfordert eine neue Art der Methodenentwicklung um einen effizienten Betrieb der Lebensmittellabore zu gew\u00e4hrleisten. Umfassende Multi-Mykotoxin-Methoden w\u00e4ren eine attraktive Alternative, verglichen mit der Entwicklung separater Methoden f\u00fcr die unterschiedlichen Analytenlisten. Die Entwicklung einer einzigen Methode kann jedoch aufgrund des breiten Spektrums an chemischen Eigenschaften der unterschiedlicher Mykotoxin-Klassen schwierig sein. Insbesondere die Alternaria- Toxine und die Ergotalkaloide stellen die Methodenentwicklung vor Herausforderungen. Werden diese Kontaminanten auf einer C18-Phase analysiert, muss bei hohen pH-Werten gearbeitet werden, um eine akzeptable Peakform f\u00fcr die Alternaria-Toxine und eine angemessene Trennung der Epimere der Ergotalkaloide zu erzielen. Die Nutzung im Alkalischen ist jedoch f\u00fcr LC-S\u00e4ulen belastend und f\u00fcr die Analyse anderer Klassen von Mykotoxinen nicht geeignet, so dass f\u00fcr eine wirklich umfassende Multi-Methode ein anderer Ansatz erforderlich ist.<\/p>\n\n\n\n<p>Hier beschreiben wir wie Altarnaria Toxine, Ergotalkaloid-Epimere und andere h\u00e4ufig vorkommende Mykotoxine gleichzeitig analysiert werden mittels einer einfachen Probenvorbereitung und einer Trennung im Sauren auf einer Raptor Biphenyl-S\u00e4ule. Unter diesen S\u00e4ulenund MS-kompatiblen Bedingungen konnte in einem schnellen Lauf mit einer Gesamtzykluszeit von 11 Minuten, 37 regulierte und neu aufgetretene Mykotoxine quantitativ analysiert werden (einschlie\u00dflich der Alternaria-Toxine und aller sechs essentiellen Ergotalkaloide und deren Epimere). In einer separaten Studie wurde die Leistungsf\u00e4higkeit der Methode in Weizen-Babynahrung, Erdn\u00fcssen, Tomatenp\u00fcree und Mischmehl verifiziert, um die Anwendbarkeit dieser Methode f\u00fcr eine Vielzahl von Lebensmitteln zu demonstrieren [1]. Die Quantifizierung wurde mit matrixangepassten Standards durchgef\u00fchrt, da f\u00fcr die meisten Mykotoxine keine isotopenmarkierten Standards zur Verf\u00fcgung standen. Die Verwendung unserer Multi-Mykotoxin-Methode auf der Raptor Biphenyl erm\u00f6glicht es Lebensmittellaboren ihre Produktivit\u00e4t deutlich zu steigern &#8211; verglichen mit der Verwendung separater Methoden f\u00fcr unterschiedliche Mykotoxin-Panels.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Abbildung 1:<\/strong>\u00a0Steigern Sie Ihre Produktivit\u00e4t durch Verwendung einer einzigen Multi-Mykotoxin-Methode f\u00fcr die\u00a0gleichzeitige Analyse von 37 wichtiger Mykotoxine, einschlie\u00dflich der Alternaria-Toxine und der Ergotalkaloid-Epimere\u00a0(Probe aus Mischmehl).<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0550.png\" alt=\"Alternaria Toxins, Ergot Alkaloid Epimers, and Other Major Mycotoxins in Fortified Flour Sample on Raptor Biphenyl by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0550<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nivalenol \" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/23282-20-4\/Nivalenol \" target=\"_blank\" rel=\"noopener\">Nivalenol <\/a><\/td><td class=\"oth\">0.92<\/td><td class=\"oth\">295.1<\/td><td class=\"oth\">137.1<\/td><td class=\"oth\">91.0<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Patulin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/149-29-1\/Patulin\" target=\"_blank\" rel=\"noopener\">Patulin<\/a><\/td><td class=\"oth\">1.03<\/td><td class=\"oth\">155.0<\/td><td class=\"oth\">99.0<\/td><td class=\"oth\">81.0<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergometrine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/60-79-7\/Ergometrine\" target=\"_blank\" rel=\"noopener\">Ergometrine<\/a><\/td><td class=\"oth\">1.27<\/td><td class=\"oth\">326.2<\/td><td class=\"oth\">223.2<\/td><td class=\"oth\">208.1<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Deoxynivalenol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/51481-10-8\/Deoxynivalenol\" target=\"_blank\" rel=\"noopener\">Deoxynivalenol<\/a><\/td><td class=\"oth\">1.30<\/td><td class=\"oth\">297.2<\/td><td class=\"oth\">231.0<\/td><td class=\"oth\">249.0<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergometrinine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/479-00-5\/Ergometrinine\" target=\"_blank\" rel=\"noopener\">Ergometrinine<\/a><\/td><td class=\"oth\">1.83<\/td><td class=\"oth\">326.2<\/td><td class=\"oth\">223.2<\/td><td class=\"oth\">208.1<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Fusarenon-X\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/23255-69-8\/Fusarenon-X\" target=\"_blank\" rel=\"noopener\">Fusarenon-X<\/a><\/td><td class=\"oth\">1.98<\/td><td class=\"oth\">355.1<\/td><td class=\"oth\">137.1<\/td><td class=\"oth\">247.1<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 15-Acetyldeoxynivalenol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/88337-96-6\/15-Acetyldeoxynivalenol\" target=\"_blank\" rel=\"noopener\">15-Acetyldeoxynivalenol<\/a><\/td><td class=\"oth\">3.14<\/td><td class=\"oth\">339.2<\/td><td class=\"oth\">137.1<\/td><td class=\"oth\">321.2<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\">3-Acetyldeoxynivalenol<\/td><td class=\"oth\">3.21<\/td><td class=\"oth\">339.2<\/td><td class=\"oth\">213.1<\/td><td class=\"oth\">231.1<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\">Tenuazonic acid<\/td><td class=\"oth\">4.22<\/td><td class=\"oth\">198.1<\/td><td class=\"oth\">125.0<\/td><td class=\"oth\">153.1<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Altenuene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/29752-43-0\/Altenuene\" target=\"_blank\" rel=\"noopener\">Altenuene<\/a><\/td><td class=\"oth\">4.70<\/td><td class=\"oth\">293.2<\/td><td class=\"oth\">257.1<\/td><td class=\"oth\">275.2<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Alternariol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/641-38-3\/Alternariol\" target=\"_blank\" rel=\"noopener\">Alternariol<\/a><\/td><td class=\"oth\">5.30<\/td><td class=\"oth\">259.0<\/td><td class=\"oth\">185.1<\/td><td class=\"oth\">130.0<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Citrinin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/518-75-2\/Citrinin\" target=\"_blank\" rel=\"noopener\">Citrinin<\/a><\/td><td class=\"oth\">5.43<\/td><td class=\"oth\">251.2<\/td><td class=\"oth\">233.1<\/td><td class=\"oth\">205.1<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergosine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/561-94-4\/Ergosine\" target=\"_blank\" rel=\"noopener\">Ergosine<\/a><\/td><td class=\"oth\">5.47<\/td><td class=\"oth\">548.4<\/td><td class=\"oth\">208.1<\/td><td class=\"oth\">223.2<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\">Fumonisin B1<\/td><td class=\"oth\">5.63<\/td><td class=\"oth\">722.5<\/td><td class=\"oth\">352.3<\/td><td class=\"oth\">334.2<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergosinine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/596-88-3\/Ergosinine\" target=\"_blank\" rel=\"noopener\">Ergosinine<\/a><\/td><td class=\"oth\">5.67<\/td><td class=\"oth\">548.4<\/td><td class=\"oth\">208.1<\/td><td class=\"oth\">223.2<\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Diacetoxyscirpenol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2270-40-8\/Diacetoxyscirpenol\" target=\"_blank\" rel=\"noopener\">Diacetoxyscirpenol<\/a><\/td><td class=\"oth\">5.73<\/td><td class=\"oth\">384.2<\/td><td class=\"oth\">247.1<\/td><td class=\"oth\">307.2<\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergotamine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/113-15-5\/Ergotamine\" target=\"_blank\" rel=\"noopener\">Ergotamine<\/a><\/td><td class=\"oth\">5.90<\/td><td class=\"oth\">582.4<\/td><td class=\"oth\">223.2<\/td><td class=\"oth\">268.2<\/td><\/tr>\n<tr><td class=\"num\">18.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergocornine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/564-36-3\/Ergocornine\" target=\"_blank\" rel=\"noopener\">Ergocornine<\/a><\/td><td class=\"oth\">6.03<\/td><td class=\"oth\">562.4<\/td><td class=\"oth\">268.2<\/td><td class=\"oth\">223.2<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">19.<\/td><td class=\"cmpd\">Ergotaminine<\/td><td class=\"oth\">6.13<\/td><td class=\"oth\">582.4<\/td><td class=\"oth\">223.2<\/td><td class=\"oth\">268.2<\/td><\/tr>\n<tr><td class=\"num\">20.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for HT-2\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/26934-87-2\/HT-2\" target=\"_blank\" rel=\"noopener\">HT-2<\/a><\/td><td class=\"oth\">6.20<\/td><td class=\"oth\">447.2<\/td><td class=\"oth\">345.1<\/td><td class=\"oth\">285.1<\/td><\/tr>\n<tr><td class=\"num\">21.<\/td><td class=\"cmpd\">Fumonisin B3<\/td><td class=\"oth\">6.32<\/td><td class=\"oth\">706.4<\/td><td class=\"oth\">336.2<\/td><td class=\"oth\">318.3<\/td><\/tr>\n<tr><td class=\"num\">22.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergocryptine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/511-09-1\/Ergocryptine\" target=\"_blank\" rel=\"noopener\">Ergocryptine<\/a><\/td><td class=\"oth\">6.32<\/td><td class=\"oth\">576.4<\/td><td class=\"oth\">268.2<\/td><td class=\"oth\">223.2<\/td><\/tr>\n<tr><td class=\"num\">23.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergocristine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/511-08-0\/Ergocristine\" target=\"_blank\" rel=\"noopener\">Ergocristine<\/a><\/td><td class=\"oth\">6.56<\/td><td class=\"oth\">610.4<\/td><td class=\"oth\">223.2<\/td><td class=\"oth\">592.4<\/td><\/tr>\n<tr><td class=\"num\">24.<\/td><td class=\"cmpd\">Fumonisin B2<\/td><td class=\"oth\">6.68<\/td><td class=\"oth\">706.4<\/td><td class=\"oth\">336.2<\/td><td class=\"oth\">318.3<\/td><\/tr>\n<tr><td class=\"num\">25.<\/td><td class=\"cmpd\">Tentoxin<\/td><td class=\"oth\">6.70<\/td><td class=\"oth\">415.2<\/td><td class=\"oth\">312.2<\/td><td class=\"oth\">302.2<\/td><\/tr>\n<tr><td class=\"num\">26.<\/td><td class=\"cmpd\">\u03b1-Zearalenol<\/td><td class=\"oth\">6.96<\/td><td class=\"oth\">303.1<\/td><td class=\"oth\">285.1<\/td><td class=\"oth\">175.0<\/td><\/tr>\n<tr><td class=\"num\">27.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergocorninine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/564-37-4\/Ergocorninine\" target=\"_blank\" rel=\"noopener\">Ergocorninine<\/a><\/td><td class=\"oth\">7.07<\/td><td class=\"oth\">562.4<\/td><td class=\"oth\">268.2<\/td><td class=\"oth\">223.2<\/td><\/tr>\n<tr><td class=\"num\">28.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for T-2\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/50-31-7\/T-2\" target=\"_blank\" rel=\"noopener\">T-2<\/a><\/td><td class=\"oth\">7.14<\/td><td class=\"oth\">489.2<\/td><td class=\"oth\">387.1<\/td><td class=\"oth\">245.1<\/td><\/tr>\n<tr><td class=\"num\">29.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Aflatoxin G2\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/7241-98-7\/Aflatoxin G2\" target=\"_blank\" rel=\"noopener\">Aflatoxin G2<\/a><\/td><td class=\"oth\">7.15<\/td><td class=\"oth\">331.2<\/td><td class=\"oth\">189.0<\/td><td class=\"oth\">313.0<\/td><\/tr>\n<tr><td class=\"num\">30.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergocryptinine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/511-10-4\/Ergocryptinine\" target=\"_blank\" rel=\"noopener\">Ergocryptinine<\/a><\/td><td class=\"oth\">7.31<\/td><td class=\"oth\">576.4<\/td><td class=\"oth\">268.2<\/td><td class=\"oth\">223.2<\/td><\/tr>\n<tr><td class=\"num\">31.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergocristinine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/511-07-9\/Ergocristinine\" target=\"_blank\" rel=\"noopener\">Ergocristinine<\/a><\/td><td class=\"oth\">7.53<\/td><td class=\"oth\">610.4<\/td><td class=\"oth\">223.2<\/td><td class=\"oth\">592.4<\/td><\/tr>\n<tr><td class=\"num\">32.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Aflatoxin G1\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1165-39-5\/Aflatoxin G1\" target=\"_blank\" rel=\"noopener\">Aflatoxin G1<\/a><\/td><td class=\"oth\">7.62<\/td><td class=\"oth\">329.1<\/td><td class=\"oth\">199.7<\/td><td class=\"oth\">243.0<\/td><\/tr>\n<tr><td class=\"num\">33.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Zearalenone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/17924-92-4\/Zearalenone\" target=\"_blank\" rel=\"noopener\">Zearalenone<\/a><\/td><td class=\"oth\">7.65<\/td><td class=\"oth\">319.2<\/td><td class=\"oth\">283.1<\/td><td class=\"oth\">187.0<\/td><\/tr>\n<tr><td class=\"num\">34.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Alternariol monomethylether\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/26894-49-5\/Alternariol monomethylether\" target=\"_blank\" rel=\"noopener\">Alternariol monomethylether<\/a><\/td><td class=\"oth\">7.69<\/td><td class=\"oth\">273.0<\/td><td class=\"oth\">199.1<\/td><td class=\"oth\">128.0<\/td><\/tr>\n<tr><td class=\"num\">35.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Aflatoxin B2\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/7220-81-7\/Aflatoxin B2\" target=\"_blank\" rel=\"noopener\">Aflatoxin B2<\/a><\/td><td class=\"oth\">7.81<\/td><td class=\"oth\">315.1<\/td><td class=\"oth\">287.0<\/td><td class=\"oth\">259.0<\/td><\/tr>\n<tr><td class=\"num\">36.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Aflatoxin B1\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1162-65-8\/Aflatoxin B1\" target=\"_blank\" rel=\"noopener\">Aflatoxin B1<\/a><\/td><td class=\"oth\">8.20<\/td><td class=\"oth\">313.2<\/td><td class=\"oth\">241.1<\/td><td class=\"oth\">284.9<\/td><\/tr>\n<tr><td class=\"num\">37.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ochratoxin A\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/303-47-9\/Ochratoxin A\" target=\"_blank\" rel=\"noopener\">Ochratoxin A<\/a><\/td><td class=\"oth\">8.31<\/td><td class=\"oth\">404.1<\/td><td class=\"oth\">239.0<\/td><td class=\"oth\">358.0<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Biphenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0550\" rel=\"noopener\">cat.# 9309A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309A0252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0550\" rel=\"noopener\">cat.# 9309A0252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>60 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><td><\/td><td>Aflatoxins standard (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/34121?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0550\" rel=\"noopener\">cat.# 34121<\/a>)<\/td><\/tr><tr><td><\/td><td>Ochratoxin A standard (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/34122?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0550\" rel=\"noopener\">cat.# 34122<\/a>)<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>50:50 Water:methanol<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>6.25 ng\/mL final concentration after sample preparation<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 0.05% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol, 0.05% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>75<\/td><td>25<\/td><\/tr><tr><td>5.00<\/td><td>0.4<\/td><td>50<\/td><td>50<\/td><\/tr><tr><td>9.00<\/td><td>0.4<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>9.01<\/td><td>0.4<\/td><td>75<\/td><td>25<\/td><\/tr><tr><td>11.0<\/td><td>0.4<\/td><td>75<\/td><td>25<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>A blended flour was prepared by mixing white rice flour (75%); brown rice flour (5%); millet flour (5%); oat flour (5%); all-purpose wheat flour (5%); and all-purpose, gluten-free flour (5%). Two grams of the flour sample were weighed into a 50-mL polypropylene centrifuge tube (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/25846?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0550\" rel=\"noopener\">cat.# 25846<\/a>) and fortified at 50 \u00b5g\/kg for all analytes with a stock standard solution. After sitting at room temperature for 10 minutes, 16 mL of extraction solution (80:20 acetonitrile:water) containing 0.5% formic acid were added, and the tube was stirred to create a homogenous suspension. The extraction was carried out by shaking horizontally on a digital pulse mixer (Glas-Col LLC, Terre Haute, IN) at 800 rpm for 20 minutes. After centrifuging for 5 minutes at 4000 rpm, 1 mL of extract was evaporated to dryness at 45 \u00b0C under a gentle stream of nitrogen. The dried extract was reconstituted with 1 mL of 50:50 water:methanol solution, and a 0.4 mL aliquot was transferred to and filtered using a Thomson SINGLE StEP filter vial with a 0.2 \u00b5m PTFE filter cat.# 25874. Five \u00b5L of the filtered solution was injected for the LC-MS\/MS analysis.<br \/><br \/><\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>The chromatogram shows peaks with the MS transition of product ion 1.<br \/><br \/>Note that method development work demonstrated that whenever a new column is installed it must be rinsed and maintained under mobile phase overnight to ensure an acceptable peak shape for tenuazonic acid.  <br \/><br \/>Want even better performance when analyzing metal-sensitive compounds? Check out Inert LC columns at <a href=\"https:\/\/www.restek.com\/inert\" target=\"_blank\" rel=\"noopener\">www.restek.com\/inert<\/a>.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0550.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Literaturhinweise<\/h2>\n\n\n\n<ol class=\"wp-block-list\">\n<li>H. Liang, J. York, J. Konschnik, H. Majer, J. Steimling,\u00a0<a href=\"https:\/\/discover.restek.com\/fsan3903\/comprehensive-mycotoxin-analysis-simultaneous-determination-of-alternaria-toxins-ergot-alkaloid-epimers-and-other-major-mycotoxins-in-various-food-matrices-by-lc-msms\/\" data-type=\"post\" data-id=\"39931\">Comprehensive mycotoxin analysis: simultaneous determination of\u00a0<em>Alternaria<\/em>\u00a0toxins, ergot alkaloid epimers, and other major mycotoxins in various food matrices by LC-MS\/MS<\/a>, Restek Corporation, 2022.<\/li>\n<\/ol>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\" style=\"background-color:#e4f7fa\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<h3 class=\"wp-block-heading has-text-color has-link-color wp-elements-a2146f03a7f97edb6f8e68cca10e9ab8\" style=\"color:#02366d\">Wollen Sie noch bessere Ergebnisse bei der Analyse von Mykotoxinen und anderen metall-sensitiven Verbindungen erzielen?<\/h3>\n\n\n\n<p>Erfahren Sie mehr unter&nbsp;<a href=\"https:\/\/www.restek.com\/articles\/accurately-analyze-metal-sensitive-compounds-with-resteks-new-inert-lc-columns\" target=\"_blank\" rel=\"noopener\">www.restek.com\/inert<\/a><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"400\" height=\"123\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/teaser-product-lc-columns-inert-lc-02.png\" alt=\"\" class=\"wp-image-29767\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/teaser-product-lc-columns-inert-lc-02.png 400w, https:\/\/discover.restek.com\/wp-content\/uploads\/teaser-product-lc-columns-inert-lc-02-300x92.png 300w\" sizes=\"auto, (max-width: 400px) 100vw, 400px\" \/><\/figure>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Labore, die Mykotoxine in Lebensmitteln analysieren, k\u00f6nnen mit dieser Methode zur gleichzeitigen quantitativen Analyse von 37 regulierten und neu aufkommenden Mykotoxinen &#8211; darunter Alternaria-Toxine, Mutterkorn-Alkaloid-Epimere und andere weitverbreitete Mykotoxine &#8211; ihre Produktivit\u00e4t steigern.<\/p>\n","protected":false},"author":11,"featured_media":7036,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[787],"tags":[],"industries-application":[2236,2238,2229],"post-badge":[],"resource-type":[],"product-library":[2481,2499],"resource-technique":[2326,2363],"hf_cat_post":[650],"ppma_author":[414],"class_list":["post-43615","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-artikel","industries-application-lebensmittel-getranke","industries-application-lebensmittelsicherheit","industries-application-raffinerie","product-library-flussigchromatographie-produkte","product-library-lc-saulen","resource-technique-flussigchromatografie","resource-technique-ms-ms-de"],"acf":[],"taxonomy_info":{"category":[{"value":787,"label":"Artikel"}],"industries-application":[{"value":2236,"label":"Lebensmittel &amp; 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