{"id":43730,"date":"2025-10-03T21:36:16","date_gmt":"2025-10-03T21:36:16","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/2-3x-faster-nitrosamines-analysis-with-lpgc-ms\/"},"modified":"2025-12-02T16:01:48","modified_gmt":"2025-12-02T16:01:48","slug":"2-3x-faster-nitrosamines-analysis-with-lpgc-ms","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/de\/artikel\/gnfa4240\/2-3x-faster-nitrosamines-analysis-with-lpgc-ms","title":{"rendered":"2.3x Faster Nitrosamines Analysis with LPGC-MS"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Up to 2.3x faster than conventional GC-MS analysis.<\/li>\n\n\n\n<li>Reduces helium usage by up to 67% compared to conventional GC-MS methods.&nbsp;<\/li>\n\n\n\n<li>Rxi-624Sil MS kits are factory connected with a proven leak-free connector making LPGC as simple as a column change.<\/li>\n<\/ul>\n\n\n\n<p>Nitrosamines are widespread impurities that are prevalent in food products, including processed foods, cured meats, and vegetables, along with cosmetics, drinking water, and the air. Due to the carcinogenic potential of these compounds at low levels, the FDA, in collaboration with other regulatory authorities, provides guidance on the acceptable daily limit (ADL) for nitrosamines. Accurate and efficient methods are needed to monitor nitrosamine levels across food and consumable products and the environment. GC methodologies for nitrosamines are frequently performed using 30 m 624 type capillary columns and require high amounts of helium, a costly and nonrenewable resource. To reduce helium usage and analysis times, shorter or thinner-film columns are an attractive alternative; however, these column types are not recommended for use with ion-trap GC-MS\/MS systems because the chromatographic peak widths generated are insufficient to obtain a necessary number of scans during peak elution.<\/p>\n\n\n\n<p>Our low-pressure GC (LPGC) column kits provide an advantage in nitrosamine analysis by reducing both analysis time and helium usage and are available in two different configurations to accommodate the unique testing requirements of each laboratory. The LPGC-MS method shown in Figure 1 below, run on our 10 m x 0.32 mm ID LPGC Rxi-624 Sil MS kit (cat.# 11804), increased the speed of analysis by 2.3x and reduced helium consumption by 67% compared to conventional GC-MS methods. Our 15 m x 0.53 mm ID LPGC Rxi-624 Sil MS kit (cat.# 11803) is the recommended kit for laboratories performing large volume injections and can be used out of the box without the hassle of attaching a guard column. Utilizing LPGC kit, cat.# 11803, the LPGC method was 1.8x faster with a 29% reduction in helium consumption (shown in Figure 2). LPGC is an opportunity for laboratories to increase the efficiency of current methodologies and reduce laboratory costs and is as simple as a column change. Additionally, all our LPGC column kits are factory coupled and individually tested, so you can have the assurance of a leak-free connection. Learn more about LPGC at\u00a0<a href=\"https:\/\/www.restek.com\/articles\/an-introduction-to-low-pressure-GC-MS-LPGC-MS\" target=\"_blank\" rel=\"noopener\">www.restek.com\/lpgc<\/a><\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 1:<\/strong> Comparison of Conventional and LPGC-MS (cat.# 11804) Analysis of Nitrosamines<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/gc_fs0598.png\" alt=\"Comparison of Conventional and LPGC-MS (0.32 mm ID) Analysis of Nitrosamines\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> GC_FS0598<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (30 m)<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (LPGC)<\/th><th style=\"text-align: center;width: 75px\">Conc. (ppm)<\/th><th style=\"text-align: center;width: 75px\">Ions<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">&lt;a class=&quot;cmpd_link&quot; title=&quot;View compound information for <i>N<\/i>-Nitrosodimethylamine (NDMA)&#8221; title=&#8221;View compound information for <i>N<\/i>-Nitrosodimethylamine (NDMA)&#8221; href=&#8221;https:\/\/ez.restek.com\/compound\/view\/en\/62-75-9\/<i>N-Nitrosodimethylamine&#8221;&gt;<i>N<\/i>-Nitrosodimethylamine (NDMA)<\/a><\/td><td class=\"oth\">4.59<\/td><td class=\"oth\">1.70<\/td><td class=\"oth\">100<\/td><td class=\"oth\">42, 74<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\">&lt;a class=&quot;cmpd_link&quot; title=&quot;View compound information for <i>N<\/i>-Nitrosodimethylamine-d6 (NDMA-d6)&#8221; title=&#8221;View compound information for <i>N<\/i>-Nitrosodimethylamine-d6 (NDMA-d6)&#8221; href=&#8221;https:\/\/ez.restek.com\/compound\/view\/en\/17829-05-9\/<i>N-Nitrosodimethylamine-d6&#8243;&gt;<i>N<\/i>-Nitrosodimethylamine-d6 (NDMA-d6)<\/a><\/td><td class=\"oth\">4.60<\/td><td class=\"oth\">1.71<\/td><td class=\"oth\">100<\/td><td class=\"oth\">46, 80<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\">&lt;a class=&quot;cmpd_link&quot; title=&quot;View compound information for <i>N<\/i>-Nitrosomethylethylamine (NMEA)&#8221; title=&#8221;View compound information for <i>N<\/i>-Nitrosomethylethylamine (NMEA)&#8221; href=&#8221;https:\/\/ez.restek.com\/compound\/view\/en\/10595-95-6\/<i>N-Nitrosomethylethylamine&#8221;&gt;<i>N<\/i>-Nitrosomethylethylamine (NMEA)<\/a><\/td><td class=\"oth\">5.18<\/td><td class=\"oth\">2.02<\/td><td class=\"oth\">100<\/td><td class=\"oth\">42, 88<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\">&lt;a class=&quot;cmpd_link&quot; title=&quot;View compound information for <i>N<\/i>-Nitrosodiethylamine (NDEA)&#8221; title=&#8221;View compound information for <i>N<\/i>-Nitrosodiethylamine (NDEA)&#8221; href=&#8221;https:\/\/ez.restek.com\/compound\/view\/en\/55-18-5\/<i>N-Nitrosodiethylamine&#8221;&gt;<i>N<\/i>-Nitrosodiethylamine (NDEA)<\/a><\/td><td class=\"oth\">5.65<\/td><td class=\"oth\">2.26<\/td><td class=\"oth\">100<\/td><td class=\"oth\">42, 57, 102<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\">&lt;a class=&quot;cmpd_link&quot; title=&quot;View compound information for <i>N<\/i>-Nitroso-di-n-propylamine (NDPA)&#8221; title=&#8221;View compound information for <i>N<\/i>-Nitroso-di-n-propylamine (NDPA)&#8221; href=&#8221;https:\/\/ez.restek.com\/compound\/view\/en\/621-64-7\/<i>N-Nitroso-di-n-propylamine&#8221;&gt;<i>N<\/i>-Nitroso-di-n-propylamine (NDPA)<\/a><\/td><td class=\"oth\">6.71<\/td><td class=\"oth\">2.81<\/td><td class=\"oth\">100<\/td><td class=\"oth\">70, 113, 130<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\">&lt;a class=&quot;cmpd_link&quot; title=&quot;View compound information for <i>N<\/i>-Nitrosopyrrolidine (NPYR)&#8221; title=&#8221;View compound information for <i>N<\/i>-Nitrosopyrrolidine (NPYR)&#8221; href=&#8221;https:\/\/ez.restek.com\/compound\/view\/en\/930-55-2\/<i>N-Nitrosopyrrolidine&#8221;&gt;<i>N<\/i>-Nitrosopyrrolidine (NPYR)<\/a><\/td><td class=\"oth\">6.87<\/td><td class=\"oth\">2.87<\/td><td class=\"oth\">100<\/td><td class=\"oth\">41, 68, 100<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\">&lt;a class=&quot;cmpd_link&quot; title=&quot;View compound information for <i>N<\/i>-Nitrosopiperidine (NPIP)&#8221; title=&#8221;View compound information for <i>N<\/i>-Nitrosopiperidine (NPIP)&#8221; href=&#8221;https:\/\/ez.restek.com\/compound\/view\/en\/100-75-4\/<i>N-Nitrosopiperidine&#8221;&gt;<i>N<\/i>-Nitrosopiperidine (NPIP)<\/a><\/td><td class=\"oth\">7.10<\/td><td class=\"oth\">2.98<\/td><td class=\"oth\">100<\/td><td class=\"oth\">42, 55, 114<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\">&lt;a class=&quot;cmpd_link&quot; title=&quot;View compound information for <i>N<\/i>-Nitrosodi-n-butylamine (NDBA)&#8221; title=&#8221;View compound information for <i>N<\/i>-Nitrosodi-n-butylamine (NDBA)&#8221; href=&#8221;https:\/\/ez.restek.com\/compound\/view\/en\/924-16-3\/<i>N-Nitrosodi-n-butylamine&#8221;&gt;<i>N<\/i>-Nitrosodi-n-butylamine (NDBA)<\/a><\/td><td class=\"oth\">7.74<\/td><td class=\"oth\">3.36<\/td><td class=\"oth\">100<\/td><td class=\"oth\">84, 116, 158<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>See notes<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><td><\/td><td>Nitrosamine calibration mix, method 521 (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/31898?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_FS0598\" rel=\"noopener\">cat.# 31898<\/a>)<\/td><\/tr><tr><td><\/td><td><i>N<\/i>-Nitrosodimethylamine-d6 (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/33910?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_FS0598\" rel=\"noopener\">cat.# 33910<\/a>)<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Dichloromethane<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>100 \u00b5g\/mL<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Injection<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>1 \u00b5L split (split ratio 100:1)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Liner:<\/th><td>Topaz, Precision inlet liner, 4.0 mm x 6.3 x 78.5 (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/23305?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_FS0598\" rel=\"noopener\">cat.# 23305<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Temp.:<\/th><td>250 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Carrier Gas<\/th><td>He<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>SIM<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Transfer Line Temp.:<\/th><td>280 \u00b0C<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Analyzer Type:<\/th><td>Quadrupole <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Source Temp.:<\/th><td>230 \u00b0C<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Quad Temp.:<\/th><td>150 \u00b0C<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Tune Type:<\/th><td>PFTBA <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ionization Mode:<\/th><td>EI <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Agilent 7890B GC &amp; 5977A MSD<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>100 \u00b5L aliquots of each standard were diluted in 800 \u00b5L of dichloromethane for a final volume of 1 mL. The sample was mixed in a 2 mL, short-cap, screw-thread vial (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/21143?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_FS0598\" rel=\"noopener\">cat.# 21143<\/a>) and capped with a short-cap, screw-vial closure (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/24495?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_FS0598\" rel=\"noopener\">cat.# 24495<\/a>).<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td><b>Conventional (30 m) Analysis:<\/b><br \/>Column: Rxi-624Sil MS, 30 m, 0.25 mm ID, 1.4 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/13868?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_FS0598\" rel=\"noopener\">cat.# 13868<\/a>)<br \/>Temp. program: 40 \u00b0C (hold 0.5 min) to 320 \u00b0C at 30 \u00b0C\/min (hold 7 min)<br \/>Flow: 1.4 mL\/min<br \/><br \/><b>LPGC-MS Analysis:<\/b><br \/>Column: LPGC Rxi-624Sil MS column kit, includes 10 m x 0.32 mm ID x 1.8 \u03bcm Rxi-624Sil MS analytical column and 5 m x 0.15 mm ID Rxi restrictor factory connected via SilTite connector (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/11804?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_FS0598\" rel=\"noopener\">cat.# 11804<\/a>)<br \/>Temp. program: 40 \u00b0C (hold 0.5 min) to 300 \u00b0C at 55 \u00b0C\/min (hold 3 min)<br \/>Flow: 0.9 mL\/min<br \/><br \/>The injections were performed on different instruments under different head pressures, resulting in different analyte responses.<br \/><br \/>For nitrosamine analysis, we also recommend Resprep SPE cartridges (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/26032?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_FS0598\" rel=\"noopener\">cat.# 26032<\/a>) for sample preparation. <\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/gc_fs0598.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 2:<\/strong> Comparison of Conventional and LPGC-MS (cat.# 11803) Analysis of Nitrosamines<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/gc_fs0599.png\" alt=\"Comparison of Conventional and LPGC-MS (0.53 mm ID) Analysis of Nitrosamines\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> GC_FS0599<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">tR (30m)<\/th><th style=\"text-align: center;width: 75px\">tR (LPGC)<\/th><th style=\"text-align: center;width: 75px\">Conc. (ppm)<\/th><th style=\"text-align: center;width: 75px\">Ions<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">&lt;a class=&quot;cmpd_link&quot; title=&quot;View compound information for <i>N<\/i>-Nitrosodimethylamine (NDMA)&#8221; title=&#8221;View compound information for <i>N<\/i>-Nitrosodimethylamine (NDMA)&#8221; href=&#8221;https:\/\/ez.restek.com\/compound\/view\/en\/62-75-9\/<i>N-Nitrosodimethylamine&#8221;&gt;<i>N<\/i>-Nitrosodimethylamine (NDMA)<\/a><\/td><td class=\"oth\">4.587<\/td><td class=\"oth\">2.51<\/td><td class=\"oth\">100<\/td><td class=\"oth\">42, 74<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\">&lt;a class=&quot;cmpd_link&quot; title=&quot;View compound information for <i>N<\/i>-Nitrosodimethylamine-d6 (NDMA-d6)&#8221; title=&#8221;View compound information for <i>N<\/i>-Nitrosodimethylamine-d6 (NDMA-d6)&#8221; href=&#8221;https:\/\/ez.restek.com\/compound\/view\/en\/17829-05-9\/<i>N-Nitrosodimethylamine-d6&#8243;&gt;<i>N<\/i>-Nitrosodimethylamine-d6 (NDMA-d6)<\/a><\/td><td class=\"oth\">4.6<\/td><td class=\"oth\">2.51<\/td><td class=\"oth\">100<\/td><td class=\"oth\">46, 80<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\">&lt;a class=&quot;cmpd_link&quot; title=&quot;View compound information for <i>N<\/i>-Nitrosomethylethylamine (NMEA)&#8221; title=&#8221;View compound information for <i>N<\/i>-Nitrosomethylethylamine (NMEA)&#8221; href=&#8221;https:\/\/ez.restek.com\/compound\/view\/en\/10595-95-6\/<i>N-Nitrosomethylethylamine&#8221;&gt;<i>N<\/i>-Nitrosomethylethylamine (NMEA)<\/a><\/td><td class=\"oth\">5.18<\/td><td class=\"oth\">2.843<\/td><td class=\"oth\">100<\/td><td class=\"oth\">42, 88<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\">&lt;a class=&quot;cmpd_link&quot; title=&quot;View compound information for <i>N<\/i>-Nitrosodiethylamine (NDEA)&#8221; title=&#8221;View compound information for <i>N<\/i>-Nitrosodiethylamine (NDEA)&#8221; href=&#8221;https:\/\/ez.restek.com\/compound\/view\/en\/55-18-5\/<i>N-Nitrosodiethylamine&#8221;&gt;<i>N<\/i>-Nitrosodiethylamine (NDEA)<\/a><\/td><td class=\"oth\">5.646<\/td><td class=\"oth\">3.105<\/td><td class=\"oth\">100<\/td><td class=\"oth\">42, 57, 102<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\">&lt;a class=&quot;cmpd_link&quot; title=&quot;View compound information for <i>N<\/i>-Nitroso-di-n-propylamine (NDPA)&#8221; title=&#8221;View compound information for <i>N<\/i>-Nitroso-di-n-propylamine (NDPA)&#8221; href=&#8221;https:\/\/ez.restek.com\/compound\/view\/en\/621-64-7\/<i>N-Nitroso-di-n-propylamine&#8221;&gt;<i>N<\/i>-Nitroso-di-n-propylamine (NDPA)<\/a><\/td><td class=\"oth\">6.705<\/td><td class=\"oth\">3.697<\/td><td class=\"oth\">100<\/td><td class=\"oth\">70, 113, 130<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\">&lt;a class=&quot;cmpd_link&quot; title=&quot;View compound information for <i>N<\/i>-Nitrosopyrrolidine (NPYR)&#8221; title=&#8221;View compound information for <i>N<\/i>-Nitrosopyrrolidine (NPYR)&#8221; href=&#8221;https:\/\/ez.restek.com\/compound\/view\/en\/930-55-2\/<i>N-Nitrosopyrrolidine&#8221;&gt;<i>N<\/i>-Nitrosopyrrolidine (NPYR)<\/a><\/td><td class=\"oth\">6.873<\/td><td class=\"oth\">3.783<\/td><td class=\"oth\">100<\/td><td class=\"oth\">41, 68, 100<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\">&lt;a class=&quot;cmpd_link&quot; title=&quot;View compound information for <i>N<\/i>-Nitrosopiperidine (NPIP)&#8221; title=&#8221;View compound information for <i>N<\/i>-Nitrosopiperidine (NPIP)&#8221; href=&#8221;https:\/\/ez.restek.com\/compound\/view\/en\/100-75-4\/<i>N-Nitrosopiperidine&#8221;&gt;<i>N<\/i>-Nitrosopiperidine (NPIP)<\/a><\/td><td class=\"oth\">7.104<\/td><td class=\"oth\">3.912<\/td><td class=\"oth\">100<\/td><td class=\"oth\">42, 55, 114<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\">&lt;a class=&quot;cmpd_link&quot; title=&quot;View compound information for <i>N<\/i>-Nitrosodi-n-butylamine (NDBA)&#8221; title=&#8221;View compound information for <i>N<\/i>-Nitrosodi-n-butylamine (NDBA)&#8221; href=&#8221;https:\/\/ez.restek.com\/compound\/view\/en\/924-16-3\/<i>N-Nitrosodi-n-butylamine&#8221;&gt;<i>N<\/i>-Nitrosodi-n-butylamine (NDBA)<\/a><\/td><td class=\"oth\">7.736<\/td><td class=\"oth\">4.292<\/td><td class=\"oth\">100<\/td><td class=\"oth\">84, 116, 158<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>See notes<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><td><\/td><td>Nitrosamine calibration mix, method 521 (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/31898?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_FS0599\" rel=\"noopener\">cat.# 31898<\/a>)<\/td><\/tr><tr><td><\/td><td><i>N<\/i>-Nitrosodimethylamine-d6 (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/33910?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_FS0599\" rel=\"noopener\">cat.# 33910<\/a>)<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Dichloromethane<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>100 \u00b5g\/mL<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Injection<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>1 \u00b5L split (split ratio 100:1)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Liner:<\/th><td>Topaz, Precision inlet liner, 4.0 mm x 6.3 x 78.5 (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/23305?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_FS0599\" rel=\"noopener\">cat.# 23305<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Temp.:<\/th><td>250 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Carrier Gas<\/th><td>He<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>SIM<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Transfer Line Temp.:<\/th><td>280 \u00b0C<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Analyzer Type:<\/th><td>Quadrupole <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Source Temp.:<\/th><td>230 \u00b0C<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Quad Temp.:<\/th><td>150 \u00b0C<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Tune Type:<\/th><td>PFTBA <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ionization Mode:<\/th><td>EI <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Agilent 7890B GC &amp; 5977A MSD<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>100 \u00b5L aliquots of each standard were diluted in 800 \u00b5L of dichloromethane for a final volume of 1 mL. The sample was mixed in a 2 mL, short-cap, screw-thread vial (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/21143?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_FS0599\" rel=\"noopener\">cat.# 21143<\/a>) and capped with a short-cap, screw-vial closure (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/24495?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_FS0599\" rel=\"noopener\">cat.# 24495<\/a>).<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td><b>Conventional (30 m) Analysis:<\/b><br \/>Column: Rxi-624Sil MS, 30 m, 0.25 mm ID, 1.4 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/13868?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_FS0599\" rel=\"noopener\">cat.# 13868<\/a>)<br \/>Temp. program: 40 \u00b0C (hold 0.5 min) to 320 \u00b0C at 30 \u00b0C\/min (hold 7 min)<br \/>Flow: 1.4 mL\/min<br \/><br \/><b>LPGC-MS Analysis:<\/b><br \/>Column: LPGC Rxi-624Sil MS column kit, includes 15 m x 0.53 mm ID x 3.0 \u03bcm Rxi-624Sil MS analytical column and 5 m x 0.18 mm ID Rxi restrictor factory connected via SilTite connector (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/11803?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_FS0599\" rel=\"noopener\">cat.# 11803<\/a>)<br \/>Temp. program: 40 \u00b0C (hold 0.5 min) to 300 \u00b0C at 55 \u00b0C\/min (hold 3 min)<br \/>Flow: 1.5 mL\/min<br \/><br \/>The injections were performed on different instruments under different head pressures, resulting in different analyte responses.<br \/><br \/>For nitrosamine analysis, we also recommend Resprep SPE cartridges (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/26032?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_FS0599\" rel=\"noopener\">cat.# 26032<\/a>) for sample preparation. <\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/gc_fs0599.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">Products Mentioned<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/11804\">                                Catalog No. 11804                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">LPGC Rxi-624Sil MS S\u00e4ulenkit, 0.32 mm ID<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/11804\">Produkt anzeigen<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/11803\">                                Catalog No. 11803                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">LPGC Rxi-624Sil MS S\u00e4ulenkit, 0.53 mm ID<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/11803\">Produkt anzeigen<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/31898\">                                Catalog No. 31898                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Nitrosamin-Kalibriermix, Methode 521. 1000 \u03bcg\/mL, Methylenchlorid, 1 mL\/Ampulle<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/31898\">Produkt anzeigen<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/33910\">                                Catalog No. 33910                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">N-Nitrosodimethylamin-d6 Standard, 1000 \u03bcg\/mL, Methylenchlorid, 1 mL\/Ampulle<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/33910\">Produkt anzeigen<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/23305\">                                Catalog No. 23305                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Topaz, Precision Inlet Liner, 4.0 mm x 6.3 x 78.5, f\u00fcr Agilent GCs, mit Quarzwolle, Premium-Deaktivierung, 5er Pack<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/23305\">Produkt anzeigen<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/21143\">                                Catalog No. 21143                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Short-Cap-Vial mit graduiertem Markierungsfeld, 9-425 Screw-Thread, 2.0 mL, 9 mm, 12 x 32 (nur Vial), braun, 1000er Pack<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/21143\">Produkt anzeigen<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/24495\">                                Catalog No. 24495                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Short Screw Kappen, Polypropylen, Screw-Thread, PTFE\/Silikon\/PTFE Septen, schwarz, vormontiert, 2.0 mL, 9 mm, 100er Pack<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/24495\">Produkt anzeigen<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/13868\">                                Catalog No. 13868                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Rxi-624Sil MS GC Kapillars\u00e4ule, 30 m, 0.25 mm ID, 1.40 \u00b5m<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/13868\">Produkt anzeigen<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/26032\">                                Catalog No. 26032                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">SPE-Kartusche, EPA-Methoden 521 und 522, 6 mL, 2 g, 30er Pck.<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/26032\">Produkt anzeigen<\/a>\n                                                    <\/div>\n                    <\/div>\n                            <\/div>\n        <\/div>\n        \n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>This fast analysis of nitrosamines by low-pressure GC-MS is up to 2.3x faster than conventional GC-MS analysis and offers up to 67% helium savings.<\/p>\n","protected":false},"author":11,"featured_media":7273,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[787],"tags":[],"industries-application":[2236,2238],"post-badge":[],"resource-type":[2623],"product-library":[2479,2480,2488,2492,2494,2482,2483,2513],"resource-technique":[2327],"hf_cat_post":[650,2624],"ppma_author":[414],"class_list":["post-43730","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-artikel","industries-application-lebensmittel-getranke","industries-application-lebensmittelsicherheit","resource-type-featured-application","product-library-produkte-zur-luft-gasprobenahme","product-library-gaschromatographie-produkte","product-library-gasstandards-zubehor","product-library-gc-saulen","product-library-inlet-zubehor","product-library-referenzstandards","product-library-produkte-zur-probenvorbereitung","product-library-vials-zubehor","resource-technique-low-pressure-gc-lpgc-saulen-kit"],"acf":[],"taxonomy_info":{"category":[{"value":787,"label":"Artikel"}],"industries-application":[{"value":2236,"label":"Lebensmittel 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