{"id":43751,"date":"2020-10-21T00:00:00","date_gmt":"2020-10-21T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/syringe-basics\/"},"modified":"2025-11-26T20:05:24","modified_gmt":"2025-11-26T20:05:24","slug":"syringe-basics","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/de\/articles-fr\/gnot3418\/syringe-basics","title":{"rendered":"Syringe Basics"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">Syringe Handling<\/h2>\n\n\n\n<p>When using a syringe, grasp only the syringe flange and plunger button. By doing so, variations in liquid measurement due to body heat are avoided. Pump the plunger with the syringe needle immersed in the fluid to be transferred. This will expel any trapped air in the needle and syringe. Avoid using a plunger in a dry syringe barrel.<\/p>\n\n\n\n<p>If the plunger is accidentally removed from the syringe barrel, wipe it carefully with a lint-free tissue. Reinsert the plunger into the barrel and pump deionized water or acetone through the needle and syringe. In the case of gas-tight plungers, re-wet the PTFE plunger tip prior to reinserting it into the barrel. Avoid touching the plunger because any abrasions, scratches, or oil from fingers will often interfere with proper plunger operation.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Syringe Cleaning<\/h2>\n\n\n\n<p>The life of the syringe is directly related to its cleanliness. To clean syringes, it is best to use solvents known to be effective in solvating the sample\u2014preferably those that are non-alkaline, non-phosphate, and non-detergent based. High-quality water and good-grade acetone prove to be good rinses. To clean the plunger, remove it from the syringe barrel and gently wipe it with a lint-free tissue. Reinsert the plunger into the barrel and pump deionized water or acetone through the needle and syringe. Air dry the syringe for storage. When reinserting a PTFE-tipped plunger into a syringe barrel, lubricate the tip by wetting it with deionized water or another solvent compatible with the sample.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Guide to Needle Termination Codes<\/h2>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><th>Hamilton<\/th><th>SGE<\/th><\/tr><tr><td>(N) Cemented Needle<br>(RN) Removable Needle<br>(ASN) Autosampler Cemented Needle<br>(ASRN) Autosampler Removable Needle<br>(TLL) PTFE Luer Lock<br>(KH) Knurled Hub<br>(LT) Luer Tip<br>(LTN) Luer Tip Cemented Needle<\/td><td>(F) Fixed Needle<br>(R) Removable Needle<br>(LL) Luer Lock<br>(LT) Luer Tip<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Needle Gauge Chart<\/h2>\n\n\n\n<p>Different size gauges are available for syringe needles. Restek offers a variety of gauges including 22, 22s, 23, 23s, 25, 26, 26s and the 23s\u201426s style. The small &#8220;s&#8221; (23s) after the gauge size specifies a small internal volume in the needle. For instance, a 26-gauge needle might have an internal volume of 1.25\u00b5L, when a 26s-gauge needle has a 0.26\u00b5L volume. The 26s-gauge needle has a much thicker side wall than the 26-gauge, which is more beneficial when using the syringe in an autosampler.<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th>Gauge<\/th><th>Nominal OD<\/th><th>Nominal ID<\/th><\/tr><\/thead><tbody><tr><td>26s<\/td><td>0.019 in. \/ 0.47mm<\/td><td>0.0050 in. \/ 0.13mm<\/td><\/tr><tr><td>26<\/td><td>0.018 in. \/ 0.46mm<\/td><td>0.0102 in. \/ 0.26mm<\/td><\/tr><tr><td>25<\/td><td>0.021 in. \/ 0.51mm<\/td><td>0.0102 in. \/ 0.26mm<\/td><\/tr><tr><td>23s<\/td><td>0.025 in. \/ 0.64mm<\/td><td>0.0060 in. \/ 0.15mm<\/td><\/tr><tr><td>23<\/td><td>0.025 in. \/ 0.64mm<\/td><td>0.0132 in. \/ 0.34mm<\/td><\/tr><tr><td>22s<\/td><td>0.028 in. \/ 0.72mm<\/td><td>0.0060 in. \/ 0.15mm<\/td><\/tr><tr><td>22<\/td><td>0.028 in. \/ 0.72mm<\/td><td>0.0162 in. \/ 0.41mm<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Needle Point Style<\/h2>\n\n\n\n<p>Restek offers several different point styles on&nbsp;<a href=\"https:\/\/www.restek.com\/c\/1361\" target=\"_blank\" rel=\"noopener\">syringe<\/a> and <a href=\"https:\/\/www.restek.com\/c\/1357\" target=\"_blank\" rel=\"noopener\">needle<\/a> products. Choose one based on your application.<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNOT3393-09.jpg\" alt=\"figure-article-GNOT3393-09.jpg\" width=\"296\" height=\"50\" title=\"-\"><\/td><td><strong>Point Style 2, BV:<\/strong>&nbsp;beveled needle tip. Recommended for optimum septum penetration and prevention of septum coring.<\/td><\/tr><tr><td><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNOT3393-10.jpg\" alt=\"figure-article-GNOT3393-10.jpg\" width=\"296\" height=\"50\" title=\"-\"><\/td><td><strong>Point Style 3, LC:<\/strong>&nbsp;square\/blunt needle tip. For use with HPLC injection valves and for sample pipetting.<\/td><\/tr><tr><td><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNOT3393-11.jpg\" alt=\"figure-article-GNOT3393-11.jpg\" width=\"296\" height=\"50\" title=\"-\"><\/td><td><strong>Point Style 5, S\/Hole, Bevel:<\/strong>&nbsp;conical needle with side hole. Liquid samples are filled and dispensed through the side hole.<\/td><\/tr><tr><td><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNOT3393-12.jpg\" alt=\"figure-article-GNOT3393-12.jpg\" width=\"296\" height=\"50\" title=\"-\"><\/td><td><strong>Point Style H, Dome:<\/strong>&nbsp;domed needle tip with side hole. Liquid samples are filled and dispensed through the side hole. The solid tip minimizes septum damage.<\/td><\/tr><tr><td><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNOT3393-13.jpg\" alt=\"figure-article-GNOT3393-13.jpg\" width=\"296\" height=\"50\" title=\"-\"><\/td><td><strong>Point Style Agilent, Cone, AS:<\/strong> special conical style needle point used exclusively on syringes for autosamplers.&nbsp;<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Syringe Termination<\/h2>\n\n\n\n<p>From cemented to removable needles and from PTFE luer-lock to special syringe fittings, syringe barrel terminations create the interface between a syringe and its mating connection. For your reference, we describe the most common Hamilton\/SGE terminations below.<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNOT3393-14.jpg\" alt=\"decorative\" width=\"540\" height=\"226\" title=\"-\"><\/td><td><strong>N, Cemented Needle \/ F, Fixed:<\/strong><br>Needle cemented into the glass syringe barrel at a point corresponding to the zero graduation mark. Not autoclavable.<\/td><\/tr><tr><td><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNOT3393-15.jpg\" alt=\"decorative\" width=\"540\" height=\"226\" title=\"-\"><\/td><td><strong>KH, Knurled Hub \/ R, Removable:<\/strong><br>Used on 7000 Series Hamilton syringes, exclusively. Knurled hub enables 6000 psig maximum injection pressures and the attachment of a spacer for repeatable-depth injections. Autoclavable when disassembled.<\/td><\/tr><tr><td><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNOT3393-16.jpg\" alt=\"decorative\" width=\"540\" height=\"226\" title=\"-\"><\/td><td><strong>RN, Removable Needle \/ R, Removable:<\/strong><br>Needle seats precisely to the zero graduation mark of the syringe. Allows the use of different specification needles on the same syringe barrel. Autoclavable when disassembled.<\/td><\/tr><tr><td><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNOT3393-17.jpg\" alt=\"decorative\" width=\"540\" height=\"226\" title=\"-\"><\/td><td><strong>TLL, PTFE Luer Lock \/<br>LL, Luer Lock \/ LT, Luer Tip:<\/strong><br>Male luer taper with nickel-plated brass hub accepts and locks into place luer hub needles and connectors. Autoclavable when disassembled.<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Basic education on syringes includes handling and cleaning and well as reference charts for common needle termination codes, IDs and ODs for needle gauges, and needle point styles.<\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[462],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[2447],"resource-technique":[2316,2318],"hf_cat_post":[650],"ppma_author":[414],"class_list":["post-43751","post","type-post","status-publish","format-standard","hentry","category-articles-fr","product-library-produits-pour-la-preparation-dechantillons","resource-technique-chromatographie-en-phase-gazeuse-gc","resource-technique-chromatographie-en-phase-liquide"],"acf":[],"taxonomy_info":{"category":[{"value":462,"label":"Articles"}],"product-library":[{"value":2447,"label":"Produits pour la pr\u00e9paration d'\u00e9chantillons"}],"resource-technique":[{"value":2316,"label":"Chromatographie en phase gazeuse (GC)"},{"value":2318,"label":"Chromatographie en phase liquide"}]},"featured_image_src_large":false,"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/de\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":462,"name":"Articles","slug":"articles-fr","term_group":0,"term_taxonomy_id":462,"taxonomy":"category","description":"","parent":0,"count":467,"filter":"raw","cat_ID":462,"category_count":467,"category_description":"","cat_name":"Articles","category_nicename":"articles-fr","category_parent":0}],"tag_info":false,"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/43751","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/comments?post=43751"}],"version-history":[{"count":3,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/43751\/revisions"}],"predecessor-version":[{"id":61804,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/43751\/revisions\/61804"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/media?parent=43751"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/categories?post=43751"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/tags?post=43751"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/industries-application?post=43751"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/post-badge?post=43751"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-type?post=43751"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/product-library?post=43751"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-technique?post=43751"},{"taxonomy":"hf_cat_post","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/hf_cat_post?post=43751"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/ppma_author?post=43751"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}