{"id":43813,"date":"2020-12-17T00:00:00","date_gmt":"2020-12-17T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/roc-hplc-columns\/"},"modified":"2026-02-02T23:06:33","modified_gmt":"2026-02-02T23:06:33","slug":"roc-hplc-columns","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/de\/articles\/gnss2332\/roc-hplc-columns","title":{"rendered":"Roc HPLC Columns: The Reliable Cornerstone of Your LC Lab"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li><strong>Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 400 bar HPLC system.<\/strong><\/li>\n\n\n\n<li><strong>Solid and reliable\u2014delivers the peak shape, reproducibility, ruggedness, and performance you demand.<\/strong><\/li>\n\n\n\n<li><strong>Exceptional value for routine analyses and large-volume workflows\u2014high-purity, fully porous silica backed by quality manufacturing features a variety of phases to fit most HPLC methods.<\/strong><\/li>\n\n\n\n<li><strong>Ideal for a wide range of applications from food to pharma; 5 phases cover 98% of USP methods.<\/strong><\/li>\n\n\n\n<li><strong>Newly designed minimal packaging for compact storage\u2014download serial #-specific certificates of analysis at <a href=\"https:\/\/www.restek.com\/documentation-search\" data-type=\"link\" data-id=\"https:\/\/www.restek.com\/documentation-search\" target=\"_blank\" rel=\"noopener\">restek.com\/documentation<\/a><\/strong><\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Roc Family of HPLC Phases<\/h3>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td>&nbsp;<\/td><td class=\"has-text-align-center\" data-align=\"center\"><a href=\"https:\/\/www.restek.com\/c\/1795\" target=\"_blank\" data-type=\"link\" data-id=\"https:\/\/www.restek.com\/c\/1795\" rel=\"noreferrer noopener\"><strong>C18<\/strong><\/a><br> <img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"318\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2332B-01.jpg\" alt=\"\" title=\"-\"><\/td><td class=\"has-text-align-center\" data-align=\"center\"><a href=\"https:\/\/www.restek.com\/c\/1796\" target=\"_blank\" data-type=\"link\" data-id=\"https:\/\/www.restek.com\/c\/1796\" rel=\"noreferrer noopener\"><strong>C8<\/strong><\/a><br> <img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"317\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2332B-02.jpg\" alt=\"\" title=\"-\"><\/td><td class=\"has-text-align-center\" data-align=\"center\"><a href=\"https:\/\/www.restek.com\/c\/1798\" target=\"_blank\" data-type=\"link\" data-id=\"https:\/\/www.restek.com\/c\/1798\" rel=\"noreferrer noopener\"><strong>Phenyl-Hexyl<\/strong><\/a><br> <img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"317\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2332B-03.jpg\" alt=\"\" title=\"-\"><\/td><td class=\"has-text-align-center\" data-align=\"center\"><a href=\"https:\/\/www.restek.com\/c\/1797\" target=\"_blank\" data-type=\"link\" data-id=\"https:\/\/www.restek.com\/c\/1797\" rel=\"noreferrer noopener\"><strong>Cyano<\/strong><\/a><br> <img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"317\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2332B-04.jpg\" alt=\"\" title=\"-\"><\/td><td class=\"has-text-align-center\" data-align=\"center\"><a href=\"https:\/\/www.restek.com\/c\/1799\" target=\"_blank\" data-type=\"link\" data-id=\"https:\/\/www.restek.com\/c\/1799\" rel=\"noreferrer noopener\"><strong>Silica<\/strong><\/a><br> <img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"317\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2332B-05.jpg\" alt=\"\" title=\"-\"><\/td><\/tr><tr><td><strong>USP Phase Code<\/strong><\/td><td class=\"has-text-align-left\" data-align=\"left\">L1<\/td><td>L7<\/td><td>L11<\/td><td>L10<\/td><td>L3<\/td><\/tr><tr><td><strong>Stationary Phase Category<\/strong><\/td><td class=\"has-text-align-left\" data-align=\"left\">C18, octadecylsilane<\/td><td>C8, octylsilane<\/td><td>phenyl<\/td><td>cyano<\/td><td>bare silica<\/td><\/tr><tr><td><strong>Ligand Type<\/strong><\/td><td class=\"has-text-align-left\" data-align=\"left\">monomeric C18<\/td><td>monomeric C8<\/td><td>phenyl-hexyl<\/td><td>cyanopropyl<\/td><td>n\/a<\/td><\/tr><tr><td><strong>Particle Size<\/strong><\/td><td class=\"has-text-align-left\" data-align=\"left\">3 \u03bcm or 5 \u03bcm, spherical<\/td><td>3 \u03bcm or 5 \u03bcm, spherical<\/td><td>3 \u03bcm or 5 \u03bcm, spherical<\/td><td>3 \u03bcm or 5 \u03bcm, spherical<\/td><td>3 \u03bcm or 5 \u03bcm, spherical<\/td><\/tr><tr><td><strong>Pore Size<\/strong><\/td><td class=\"has-text-align-left\" data-align=\"left\">100 \u00c5<\/td><td>100 \u00c5<\/td><td>100 \u00c5<\/td><td>100 \u00c5<\/td><td>100 \u00c5<\/td><\/tr><tr><td><strong>Surface Area<\/strong><\/td><td class=\"has-text-align-left\" data-align=\"left\">300 m<sup>2<\/sup>\/g<\/td><td>300 m<sup>2<\/sup>\/g<\/td><td>300 m<sup>2<\/sup>\/g<\/td><td>300 m<sup>2<\/sup>\/g<\/td><td>300 m<sup>2<\/sup>\/g<\/td><\/tr><tr><td><strong>Carbon Load<\/strong><\/td><td class=\"has-text-align-left\" data-align=\"left\">20%<\/td><td>12%<\/td><td>15%<\/td><td>8%<\/td><td>n\/a<\/td><\/tr><tr><td><strong>End-Cap<\/strong><\/td><td class=\"has-text-align-left\" data-align=\"left\">yes<\/td><td>yes<\/td><td>yes<\/td><td>yes<\/td><td>n\/a<\/td><\/tr><tr><td><strong>pH Range<\/strong><\/td><td class=\"has-text-align-left\" data-align=\"left\">2.5 to 8.0<\/td><td>2.5 to 8.0<\/td><td>2.0 to 8.0<\/td><td>2.5 to 8.0<\/td><td>2.5 to 8.0<\/td><\/tr><tr><td><strong>Maximum Temperature<\/strong><\/td><td class=\"has-text-align-left\" data-align=\"left\">80 \u00b0C<\/td><td>80 \u00b0C<\/td><td>80 \u00b0C<\/td><td>80 \u00b0C<\/td><td>80 \u00b0C<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 1:<\/strong>\u00a0Over 1,000 injections and Roc HPLC columns will still offer reliable performance, regardless of which dimension your method calls for.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter\"><img decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2332B-06.jpg\" alt=\"decorative\" title=\"-\"><\/figure>\n<\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 2:<\/strong>\u00a0Add a Roc HPLC guard to further extend the life of your analytical column without impacting your retention, peak shape, or efficiency.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0565.png\" alt=\"Net Chromatographic Effects of Roc C8 with Guard Column\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_GN0565<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 50px\">Conc.<br \/>(mg\/mL)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Uracil\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/66-22-8\/Uracil\" target=\"_blank\" rel=\"noopener\">Uracil<\/a><\/td><td class=\"oth\">0.02<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Toluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/108-88-3\/Toluene\" target=\"_blank\" rel=\"noopener\">Toluene<\/a><\/td><td class=\"oth\">3.00<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Naphthalene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/91-20-3\/Naphthalene\" target=\"_blank\" rel=\"noopener\">Naphthalene<\/a><\/td><td class=\"oth\">0.50<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Biphenyl\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/92-52-4\/Biphenyl\" target=\"_blank\" rel=\"noopener\">Biphenyl<\/a><\/td><td class=\"oth\">0.06<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Roc C8  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9533565?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_GN0565\" rel=\"noopener\">cat.# 9533565<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>150 mm x 4.6 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>5 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>100 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Roc C8 10 mm, 4.0 mm ID, 5 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/953350210?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_GN0565\" rel=\"noopener\">cat.# 953350210<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Custom mix<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>50:50 Acetonitrile:water<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>1.0<\/td><td>30<\/td><td>70<\/td><\/tr><tr><td>8.00<\/td><td>1.0<\/td><td>30<\/td><td>70<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>UV @ 254 nm<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>HPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0565.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 3:<\/strong>\u00a0From one column to the next, one lot to the next, Roc columns provide results you can trust time and time again.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<p class=\"has-medium-font-size\"><strong>Reversed Phase<\/strong><\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0562.png\" alt=\"Retention Time Stability for Roc C18\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_GN0562<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 50px\">t<sub>R<\/sub> (min)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for p-Tolunitrile\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/104-85-8\/p-Tolunitrile\" target=\"_blank\" rel=\"noopener\">p-Tolunitrile<\/a><\/td><td class=\"oth\">3.7<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 4-Butylbenzoic acid\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/20651-71-2\/4-Butylbenzoic acid\" target=\"_blank\" rel=\"noopener\">4-Butylbenzoic acid<\/a><\/td><td class=\"oth\">5.2<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 3,4-Dimethylanisole\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/4685-47-6\/3,4-Dimethylanisole\" target=\"_blank\" rel=\"noopener\">3,4-Dimethylanisole<\/a><\/td><td class=\"oth\">7.3<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Mefenamic acid\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/61-68-7\/Mefenamic acid\" target=\"_blank\" rel=\"noopener\">Mefenamic acid<\/a><\/td><td class=\"oth\">8.1<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for p-Xylene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/106-42-3\/p-Xylene\" target=\"_blank\" rel=\"noopener\">p-Xylene<\/a><\/td><td class=\"oth\">9.7<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Roc C18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9534565?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_GN0562\" rel=\"noopener\">cat.# 9534565<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>150 mm x 4.6 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>5 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>100 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td>0.05% Formic acid in water:0.05% Formic acid in acetonitrile (40:60)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Flow:<\/th><td>1.0 mL\/min<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>UV\/Vis @ 220 nm<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Cell Temp:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>HPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0562.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<p class=\"has-medium-font-size\"><strong>Normal Phase<\/strong><\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0563.png\" alt=\"Retention Time Stability for Roc Cyano\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_GN0563<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 50px\">t<sub>R<\/sub> (min)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Benzene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/71-43-2\/Benzene\" target=\"_blank\" rel=\"noopener\">Benzene<\/a><\/td><td class=\"oth\">3.5<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nitrobenzene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/98-95-3\/Nitrobenzene\" target=\"_blank\" rel=\"noopener\">Nitrobenzene<\/a><\/td><td class=\"oth\">4.1<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 1,3,5-Trinitrobenzene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/99-35-4\/1,3,5-Trinitrobenzene\" target=\"_blank\" rel=\"noopener\">1,3,5-Trinitrobenzene<\/a><\/td><td class=\"oth\">5.5<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2,4-Dinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/121-14-2\/2,4-Dinitrotoluene\" target=\"_blank\" rel=\"noopener\">2,4-Dinitrotoluene<\/a><\/td><td class=\"oth\">7.4<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Roc Cyano  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/953631E?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_GN0563\" rel=\"noopener\">cat.# 953631E<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 3.0 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>3 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>100 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>1 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td>Water:acetonitrile (80:20)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Flow:<\/th><td>0.6 mL\/min<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>UV\/Vis @ 254 nm<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Cell Temp:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>HPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0563.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">Roc HPLC Columns from Restek Perform Better Than or Equal To Columns from Other Vendors<\/h3>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><strong>Columns<\/strong><\/td><td><strong>Analyte<\/strong><\/td><td><strong>tR<\/strong><\/td><td><strong>Area<\/strong><\/td><td><strong>Height<\/strong><\/td><td><strong>USP Tailing<\/strong><\/td><td><strong>Asymmetry (10%)<\/strong><\/td><td><strong>k&#8217;<\/strong><\/td><td><strong>Plate Number<\/strong><\/td><td><strong>Resolution<\/strong><\/td><\/tr><tr><td rowspan=\"2\"><strong>Roc<\/strong><\/td><td>Ethylparaben<\/td><td>3.252<\/td><td>1,407,609<\/td><td>281,358<\/td><td>1.176<\/td><td>1.148<\/td><td>1.182<\/td><td>9,738<\/td><td rowspan=\"2\">12.5<\/td><\/tr><tr><td>Ethinyl Estradiol<\/td><td>5.410<\/td><td>1,869,684<\/td><td>234,346<\/td><td>1.075<\/td><td>1.064<\/td><td>2.631<\/td><td>10,264<\/td><\/tr><tr><td rowspan=\"2\"><strong>Vendor A<\/strong><\/td><td>Ethylparaben<\/td><td>3.060<\/td><td>1,424,621<\/td><td>215,698<\/td><td>1.907<\/td><td>1.608<\/td><td>1.013<\/td><td>6,732<\/td><td rowspan=\"2\">10.1<\/td><\/tr><tr><td>Ethinyl Estradiol<\/td><td>4.889<\/td><td>1,860,659<\/td><td>223,879<\/td><td>1.311<\/td><td>1.217<\/td><td>2.217<\/td><td>8,575<\/td><\/tr><tr><td rowspan=\"2\"><strong>Vendor B<\/strong><\/td><td>Ethylparaben<\/td><td>2.873<\/td><td>1,426,068<\/td><td>319,850<\/td><td>1.143<\/td><td>1.124<\/td><td>0.866<\/td><td>9,706<\/td><td rowspan=\"2\">9.9<\/td><\/tr><tr><td>Ethinyl Estradiol<\/td><td>4.307<\/td><td>1,867,049<\/td><td>287,414<\/td><td>1.064<\/td><td>1.057<\/td><td>1.797<\/td><td>10,031<\/td><\/tr><tr><td rowspan=\"2\"><strong>Vendor C<\/strong><\/td><td>Ethylparaben<\/td><td>2.763<\/td><td>1,413,099<\/td><td>315,080<\/td><td>1.262<\/td><td>1.217<\/td><td>0.932<\/td><td>8,643<\/td><td rowspan=\"2\">11.9<\/td><\/tr><tr><td>Ethinyl Estradiol<\/td><td>4.580<\/td><td>1,868,724<\/td><td>270,662<\/td><td>1.143<\/td><td>1.122<\/td><td>2.203<\/td><td>9,700<\/td><\/tr><tr><td rowspan=\"2\"><strong>Vendor D<\/strong><\/td><td>Ethylparaben<\/td><td>3.157<\/td><td>2,939,187<\/td><td>357,541<\/td><td>1.159<\/td><td>0.965<\/td><td>1.084<\/td><td>4,478<\/td><td rowspan=\"2\">12.2<\/td><\/tr><tr><td>Ethinyl Estradiol<\/td><td>5.816<\/td><td>1,771,785<\/td><td>182,056<\/td><td>1.301<\/td><td>1.232<\/td><td>2.839<\/td><td>8,954<\/td><\/tr><tr><td rowspan=\"2\"><strong>Vendor E<\/strong><\/td><td>Ethylparaben<\/td><td>3.873<\/td><td>1,498,446<\/td><td>244,642<\/td><td>1.172<\/td><td>1.134<\/td><td>1.221<\/td><td>9,348<\/td><td rowspan=\"2\">13.7<\/td><\/tr><tr><td>Ethinyl Estradiol<\/td><td>6.839<\/td><td>1,781,453<\/td><td>174,955<\/td><td>1.080<\/td><td>1.063<\/td><td>2.921<\/td><td>9,963<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 4:<\/strong>\u00a0Count on Roc HPLC columns from Restek for outstanding chromatographic performance.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<p class=\"has-medium-font-size\"><strong>Roc&nbsp;HPLC Column<\/strong><\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ex0518.png\" alt=\"Ethinyl Estradiol on Roc C18 - USP Method\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EX0518<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(\u00b5g\/mL)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ethylparaben\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/120-47-8\/Ethylparaben\" target=\"_blank\" rel=\"noopener\">Ethylparaben<\/a><\/td><td class=\"oth\">3.252<\/td><td class=\"oth\">50<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ethinyl estradiol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-63-6\/Ethinyl estradiol\" target=\"_blank\" rel=\"noopener\">Ethinyl estradiol<\/a><\/td><td class=\"oth\">5.410<\/td><td class=\"oth\">200<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ex0518.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<p class=\"has-medium-font-size\"><strong>Vendor A<\/strong><\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ex0521.png\" alt=\"Ethinyl Estradiol on Vendor A C18 - USP Method\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EX0521<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(\u00b5g\/mL)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ethylparaben\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/120-47-8\/Ethylparaben\" target=\"_blank\" rel=\"noopener\">Ethylparaben<\/a><\/td><td class=\"oth\">3.060<\/td><td class=\"oth\">50<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ethinyl estradiol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-63-6\/Ethinyl estradiol\" target=\"_blank\" rel=\"noopener\">Ethinyl estradiol<\/a><\/td><td class=\"oth\">4.889<\/td><td class=\"oth\">200<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ex0521.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<p class=\"has-medium-font-size\"><strong>Vendor B<\/strong><\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ex0520.png\" alt=\"Ethinyl Estradiol on Vendor B C18 - USP Method\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EX0520<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(\u00b5g\/mL)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ethylparaben\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/120-47-8\/Ethylparaben\" target=\"_blank\" rel=\"noopener\">Ethylparaben<\/a><\/td><td class=\"oth\">2.873<\/td><td class=\"oth\">50<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ethinyl estradiol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-63-6\/Ethinyl estradiol\" target=\"_blank\" rel=\"noopener\">Ethinyl estradiol<\/a><\/td><td class=\"oth\">4.307<\/td><td class=\"oth\">200<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ex0520.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<p class=\"has-medium-font-size\"><strong>Vendor C<\/strong><\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ex0519.png\" alt=\"Ethinyl Estradiol on Vendor C C18 - USP Method\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EX0519<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(\u00b5g\/mL)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ethylparaben\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/120-47-8\/Ethylparaben\" target=\"_blank\" rel=\"noopener\">Ethylparaben<\/a><\/td><td class=\"oth\">2.763<\/td><td class=\"oth\">50<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ethinyl estradiol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-63-6\/Ethinyl estradiol\" target=\"_blank\" rel=\"noopener\">Ethinyl estradiol<\/a><\/td><td class=\"oth\">4.580<\/td><td class=\"oth\">200<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ex0519.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<p class=\"has-medium-font-size\"><strong>Vendor D<\/strong><\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ex0522.png\" alt=\"Ethinyl Estradiol on Vendor D C18 - USP Method\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EX0522<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(\u00b5g\/mL)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ethylparaben\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/120-47-8\/Ethylparaben\" target=\"_blank\" rel=\"noopener\">Ethylparaben<\/a><\/td><td class=\"oth\">3.157<\/td><td class=\"oth\">50<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ethinyl estradiol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-63-6\/Ethinyl estradiol\" target=\"_blank\" rel=\"noopener\">Ethinyl estradiol<\/a><\/td><td class=\"oth\">5.816<\/td><td class=\"oth\">200<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ex0522.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<p class=\"has-medium-font-size\"><strong>Vendor E<\/strong><\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ex0523.png\" alt=\"Ethinyl Estradiol on Vendor E C18 - USP Method\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EX0523<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(\u00b5g\/mL)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ethylparaben\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/120-47-8\/Ethylparaben\" target=\"_blank\" rel=\"noopener\">Ethylparaben<\/a><\/td><td class=\"oth\">3.873<\/td><td class=\"oth\">50<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ethinyl estradiol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-63-6\/Ethinyl estradiol\" target=\"_blank\" rel=\"noopener\">Ethinyl estradiol<\/a><\/td><td class=\"oth\">6.839<\/td><td class=\"oth\">200<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ex0523.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<p><strong><em>Chromatographic conditions for Table I and Figure 4<\/em><\/strong><br>All columns are C18 stationary phase (5 \u00b5m, 150 x 4.6 mm). Conditions are as follows. Mobile phase: water:acetonitrile (1:1); Flow rate: 1 mL\/min; Injection volume: 25 \u00b5L; Instrument: HPLC with UV detector, 280 nm; Sample: 200 \u00b5g\/mL ethinyl estradiol and 50 \u00b5g\/mL ethylparaben in water:acetonitrile (1:1).<\/p>\n\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">Get more of what you want, and less of what you don&#8217;t, with Roc HPLC columns from Restek.<\/h3>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Solid, reliable Roc HPLC columns deliver the peak shape, reproducibility, ruggedness, and performance you demand for all of your conventional HPLC applications.<\/p>\n","protected":false},"author":11,"featured_media":26566,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[7],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[2391,2373],"resource-technique":[2299],"ppma_author":[414],"class_list":["post-43813","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles","product-library-lc-columns","product-library-liquid-chromatography-products","resource-technique-liquid-chromatography"],"acf":[],"taxonomy_info":{"category":[{"value":7,"label":"Articles"}],"product-library":[{"value":2391,"label":"LC 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