{"id":43834,"date":"2021-11-11T00:00:00","date_gmt":"2021-11-11T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/trennen-sie-eine-vielzahl-von-polaren-analyten-mit-einer-neuartigen-stationaren-hybridphase\/"},"modified":"2026-01-28T22:34:40","modified_gmt":"2026-01-28T22:34:40","slug":"trennen-sie-eine-vielzahl-von-polaren-analyten-mit-einer-neuartigen-stationaren-hybridphase","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/de\/artikel\/gnss3195-de\/trennen-sie-eine-vielzahl-von-polaren-analyten-mit-einer-neuartigen-stationaren-hybridphase","title":{"rendered":"Trennen Sie eine Vielzahl von polaren Analyten mit einer neuartigen station\u00e4ren Hybridphase"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Ohne zeitaufwendige Derivatisierung oder Ionenpaarreagenz.<\/li>\n\n\n\n<li>Wechseln Sie zwischen Retentionsmodi durch einfache \u00c4nderungen der mobilen Phase &nbsp;und kurze \u00c4quilibrierungszeiten.<\/li>\n\n\n\n<li>Ideal f\u00fcr die Erh\u00f6hung der Empfindlichkeit und Selektivit\u00e4t bei LC-MS-Analysen.<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Vereinfachen Sie die Analyse von polaren Verbindungen<\/h2>\n\n\n\n<p>Die Analyse polarer Verbindungen mithilfe der Fl\u00fcssigkeitschromatografie war in der Vergangenheit stets eine Herausforderung. Mangelnde Retention und schlechte Peakformen, komplexe mobile Phasen, die vielleicht nicht MS-vertr\u00e4glich sind, lange \u00c4quilibrierungszeiten, geringe Empfindlichkeit und die Derivatisierung der Proben sind allesamt Komplikationen, die die Effizienz und Produktivit\u00e4t der Laboratorien beeintr\u00e4chtigen. Durch die Entwicklung einer neuartigen S\u00e4ule, speziell konzipiert f\u00fcr die Analyse einer Vielzahl von polaren Verbindungen, k\u00f6nnen Wissenschaftler diese Probleme nun jedoch vermeiden, indem sie sich die wahre Kraft der Chromatografie zu Nutze machen.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Die wahre Kraft der Chromatografie nutzen<\/h2>\n\n\n\n<p>Einer der wichtigsten, aber am wenigsten verstandenen Aspekte der Leistungsf\u00e4higkeit einer Methode ist die Verwendung der korrekten station\u00e4ren Phase f\u00fcr eine bestimmte Trennung. F\u00fcr die Analyse polarer Verbindungen bieten Reversed-Phase-S\u00e4ulen keine ausreichende Retention ohne Einsatz komplexer mobiler Phasen oder Probenderivatisierung, um so die fehlenden effektiven Wechselwirkungen zwischen den Analyten und der S\u00e4ule zu kompensieren. Werden die Zielanalyten jedoch mit einer station\u00e4ren Phase mit zweckbestimmtem Aufl\u00f6sungsverm\u00f6gen gepaart, k\u00f6nnen Sie aufwendige Verfahren zu Probenvorbereitung vermeiden, Zeit und Kosten sparen und m\u00f6gliche Fehlerquellen reduzieren.<\/p>\n\n\n\n<p>Die station\u00e4re Phase der Raptor Polar X wurde speziell entwickelt, um polare Analyten durch ein Zusammenspiel von zwei Retentionsmechanismen selektiv zu retardieren. Diese spezielle Hybridphase ist ideal f\u00fcr die Analyse einer Vielzahl polarer Analyten, besonders in Verbindung mit Massenspektrometrie. Vereinfachen Sie die Analyse polarer Verbindungen mit dem Aufl\u00f6sungsverm\u00f6gen der Raptor Polar X S\u00e4ulen von Restek.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Mehr als die Summe ihrer Teile<\/h2>\n\n\n\n<p>Die bei der Analyse von polaren Verbindungen am h\u00e4ufigsten verwendeten Retentionsmechanismen sind die hydrophile Interaktionsfl\u00fcssigchromatografie (HILIC) und der Ionenaustausch. Darauf aufbauend hat Restek eine neuartige station\u00e4re Phase entwickelt, die diese beiden Modi in einem einzigen Liganden vereint. Da dieser spezielle Ligand an oberfl\u00e4chlich por\u00f6se Partikel (auch \u201eSuperficially Porous Particles\u201c oder SPP genannt) gebunden ist, l\u00e4sst sich mit Raptor Polar X S\u00e4ulen eine Vielzahl polarer Analyten zuverl\u00e4ssig retardieren und effizient trennen.<\/p>\n\n\n\n<p>Bei typischen anwendungsspezifischen S\u00e4ulen \u00fcberwiegt einer der beiden Retentionsmodi und die bei der Retention einer bestimmten polaren Verbindung erzielten Vorteile gehen zu Kosten der Trennleistung f\u00fcr andere Analyten. Im Gegensatz dazu sind bei der Raptor Polar X S\u00e4ule mit ihrer zum Patent angemeldeten Phasenchemie zwei unabh\u00e4ngige Retentionsmechanismen verf\u00fcgbar, wodurch sich eine wirklich ausgewogene und flexible Retention erzielen l\u00e4sst (Abbildung 1). Mithilfe einfacher \u00c4nderungen der mobilen Phase k\u00f6nnen Analytiker zwischen den beiden Modi wechseln und die Retention der Zielsubstanzen selektiv justieren, ohne lange \u00c4quilibrierungszeiten vor oder zwischen verschiedenen Proben zu ben\u00f6tigen.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Abbildung 1:<\/strong>\u00a0Durch einfache \u00c4nderungen der mobilen Phase l\u00e4sst sich die Raptor Polar X S\u00e4ule schnell und einfach zwischen den verschiedenen polaren Retentionsmodi wechseln. Dadurch entsteht die beispiellose M\u00f6glichkeit, eine Vielzahl polarer Verbindungen zu retardieren und trennen, selbst innerhalb des selben Analyselaufs.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<style>.kb-image43834_b84f87-03 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43834_b84f87-03\"><figure class=\"aligncenter size-medium_large\"><img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"620\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/\/figure-article-GNSS3195-01-768x620.jpg\" alt=\"\" class=\"kb-img wp-image-23305\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-01-768x620.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-01-300x242.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-01-1024x826.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-01-1536x1239.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-01.jpg 1800w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/figure><\/div>\n\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Und so funktioniert\u2018s: Wechseln des Retentionsmodus<\/h2>\n\n\n\n<p>Bei Verwendung einer mobilen Phase mit einem relativ hohen Anteil von Acetonitril zu Wasser bildet sich an der Kieselgeloberfl\u00e4che eine Wasserschicht, in der sich polare Verbindungen verteilen k\u00f6nnen. Die Verteilung in der w\u00e4ssrigen Schicht f\u00fchrt zu effektiven Wechselwirkungen zwischen polaren Analyten und dem an der Kieselgeloberfl\u00e4che gebundenen Liganden (Abbildung 2). Die neuartige Ligandenchemie der Raptor Polar X S\u00e4ule vereinfacht diese Methode weiter und erm\u00f6glicht eine schnellere \u00c4quilibrierung und Neu\u00e4quilibrierung der S\u00e4ule als je zuvor. Das hei\u00dft, neue S\u00e4ulen sind schnell betriebsbereit und der Probendurchsatz l\u00e4sst sich wegen der k\u00fcrzeren \u00c4quilibrierungszeiten zwischen verschiedenen Proben steigern.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Abbildung 2:<\/strong>\u00a0Die schnelle Bildung einer Wasserschicht an der Kieselgeloberfl\u00e4che erm\u00f6glicht die Verteilung einer Vielzahl von polaren Analyten zwischen dem Acetonitril der mobilen Phase und der w\u00e4ssrigen Schicht. Diese Verteilung, zusammen mit den Wechselwirkungen mit der station\u00e4ren Phase, definiert den HILIC-Retentionsmechanismus.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<style>.kb-image43834_e5b39b-63 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43834_e5b39b-63\"><figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"548\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/\/figure-article-GNSS3195-02-1024x548.jpg\" alt=\"\" class=\"kb-img wp-image-23311\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-02-1024x548.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-02-300x161.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-02-768x411.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-02-1536x823.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-02.jpg 1800w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure><\/div>\n\n<\/div><\/div><\/div>\n\n\n<p>F\u00fcr kleinere polare Verbindungen erzeugen diese Bedingungen mit einem hohen Anteil an Acetonitril starke Wechselwirkungen zwischen den Analyten und der station\u00e4ren Phase, so dass kleine, hochpolare geladene Verbindungen retardiert werden. Zur Feinjustierung der Retention wird einfach der Anteil des Wassers in der mobilen Phase erh\u00f6ht. Dadurch werden die geladenen polaren Verbindungen in die mobile Phase \u00fcberf\u00fchrt und effektiv eluiert. Bei Verwendung einer mobilen Phase mit h\u00f6herem Wasseranteil wird die durch HILIC-Verteilung bedingte Retention reduziert und das Ionenaustauschverhalten der station\u00e4ren Phase wird zum vorherrschenden Retentionsmechanismus (Abbildung 3).<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Abbildung 3:<\/strong>\u00a0Bei einer mobilen Phase mit hohem Wasseranteil wird der Ionenaustauschmechanismus zum vorherrschenden Retentionsmodus bei der Analyse polarer Verbindungen.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<style>.kb-image43834_4bcd8e-23 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43834_4bcd8e-23\"><figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"489\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/\/figure-article-GNSS3195-03-1024x489.jpg\" alt=\"\" class=\"kb-img wp-image-23329\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-03-1024x489.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-03-300x143.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-03-768x367.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-03-1536x734.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS3195-03.jpg 1800w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure><\/div>\n\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Wechsel des Retentionsmodus: Ein Beispiel<\/h2>\n\n\n\n<p>Zur Veranschaulichung dieser Prinzipien ist in Abbildung 4 dargestellt, wie zwei verschiedene Typen von polaren Verbindungen mit der station\u00e4ren Phase in Wechselwirkung treten und wie ihr Retentionsverhalten durch einfache \u00c4nderungen der mobilen Phase beeinflusst wird. In diesem Beispiel analysierten wir zwei wasserl\u00f6sliche Vitamine: Vitamin B3 (Niacin), eine organische S\u00e4ure, und Vitamin B1 (Thiamin), das eine permanente positive Ladung tr\u00e4gt.<\/p>\n\n\n\n<p>Im oberen Chromatogramm wurde eine mobile Phase mit hohem organischen Anteil verwendet, d.h. Bedingungen, die HILIC-Trennungen beg\u00fcnstigen. Bei Verwendung der Raptor Polar X S\u00e4ule unter diesen Bedingungen bildet sich auf der Oberfl\u00e4che der Kieselgelpartikel schnell einer Wasserschicht. Polare Verbindungen k\u00f6nnen sich dann zwischen der Acetonitrilschicht und der Wasserschicht verteilen, wo sie auch mit der Kieselgeloberfl\u00e4che und der station\u00e4ren Phase in Wechselwirkung treten k\u00f6nnen. Auf diese Weise werden polare Verbindungen durch hydrophile Wechselwirkungen mit der station\u00e4ren Phase retardiert und dennoch durch eine mobile Phase mit hohem organischen Anteil eluiert. Das beg\u00fcnstigt die LC-MS\/MS-Analyse aufgrund der besseren Desolvatisierung und der erh\u00f6hten Ionisationseffizienz.<\/p>\n\n\n\n<p>Das untere Chromatogramm illustriert die chromatografischen \u00c4nderungen, die auftreten, wenn der Retentionsmechanismus durch Verwendung einer mobilen Phase mit h\u00f6herem Wasseranteil gewechselt wird. Bei zunehmender Entfernung von den idealen HILIC-Bedingungen werden sowohl Vitamin B1 als auch Vitamin B3 weniger retardiert. Der Grund daf\u00fcr ist die zunehmende Bedeutung des Ionenaustausch-Retentionsverhaltens und die gleichzeitige Abnahme der HILIC-Retention, wenn der Wasseranteil der mobilen Phase zunimmt. Au\u00dferdem \u00e4ndert sich die Elutionsreihenfolge, weil Vitamin B1 unter Ionenaustauschbedingungen weniger retardiert wird als Vitamin B3.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Abbildung 4:<\/strong>\u00a0Einfache \u00c4nderungen der einfach herzustellenden, MS-vertr\u00e4glichen mobilen Phasen k\u00f6nnen die unterschiedlichen Retentionsmechanismen der neuartigen Raptor Polar X Phasenchemie entweder beg\u00fcnstigen oder unterdr\u00fccken.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0652.png\" alt=\"Raptor Polar X Retention Mode Comparison: Buffered B Vitamins with 95% vs. 60% Mobile Phase B\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_GN0652<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Vitamin B3\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/59-67-6\/Vitamin B3\" target=\"_blank\" rel=\"noopener\">Vitamin B3<\/a><\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Vitamin B1\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/59-43-8\/Vitamin B1\" target=\"_blank\" rel=\"noopener\">Vitamin B1<\/a><\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A52?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_GN0652\" rel=\"noopener\">cat.# 9311A52<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>0.1% Formic acid in acetonitrile<\/td><\/tr><td><\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><tr><th class=\"sub conditions_header\" scope=\"row\">Flow:<\/th><td>0.5 mL\/min<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Source:<\/th><td>Electrospray <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>HPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Mobile phase A: Water, 5 mM ammonium formate, 0.1% formic acid<br \/>Mobile phase B: Acetonitrile, 0.1% formic acid<br \/>Top chromatogram: 95% B, 10 min run, 2 \u03bcL injection (100 ppm B3, 0.01 ppm B1)<br \/>Bottom chromatogram: 60% B, 5 min run, 0.5 \u03bcL injection (100 ppm B3, 0.1 ppm B1)<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0652.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Ausgewogene Hybridretention erm\u00f6glicht Analyse mehrerer Verbindungen mit einer einzigen Methode<\/h2>\n\n\n\n<p>S\u00e4ulen sind \u00fcblicherweise spezialisiert: und zwar auf eine einzige Art von Wechselwirkung unter Ausschluss anderer. Dieser Ansatz hat sich bew\u00e4hrt, wenn die Zielanalyten identische Eigenschaften besitzen. Polare Verbindungen zeigen jedoch erhebliche Variation in ihren chemischen Eigenschaften. F\u00fcr die Analyse von polaren Verbindungen bedeutet dies h\u00e4ufig die Verwendung mehrerer Methoden mit verschiedenen S\u00e4ulen oder Bedingungen f\u00fcr jeden Verbindungstyp. Wie anhand der nachfolgenden Beispiele gezeigt, ist die Hybridphasenchemie der Raptor Polar X S\u00e4ulen eine bessere Alternative, weil das Multimode-Retentionsprofil die Analyse einer Vielzahl von Zielanalyten mit derselben Methode gestattet.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">QuPPe-inspirierte Liste polarer Verunreinigungen<\/h2>\n\n\n\n<p>Die europ\u00e4ische&nbsp;<strong>Q<\/strong>uick&nbsp;<strong>P<\/strong>olar&nbsp;<strong>P<\/strong>esticides (QuPPe)-Methode umfasst eine Reihe von polaren Analyten aus anionischen polaren Herbiziden wie Glyphosat und verwandten Verbindungen bis hin zu einer Vielzahl von Oxychlor-Verunreinigungen, darunter Chlorat und Perchlorat. Wie in Abbildung 5 gezeigt, kann die Raptor Polar X S\u00e4ule dieses diverse Gemisch retardieren und schnell trennen, wobei die letzte Verbindung nach etwa 10.5 Minuten eluiert und die Analyse insgesamt nur 13 Minuten dauert. Diese Trennung wird mit einfachen ungepufferten mobilen Phasen durchgef\u00fchrt, die mit 0,5% Ameisens\u00e4ure anges\u00e4uert werden, um Peakverbreiterung und Peaktailing zu reduzieren. Die in Abbildung 5 gezeigte Methode konnte auch Verbindungen mit \u00e4hnlichen Massenfragmenten trennen, wie etwa AMPA von N-Acetyl-AMPA und Fosetyl-Aluminium von Phosphons\u00e4ure und Phosphors\u00e4ure (eine h\u00e4ufig beobachtete Matrixkomponente und St\u00f6rquelle).<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"figure-heading\"><strong>Abbildung 5:<\/strong>\u00a0In dieser Analyse polarer Verbindungen l\u00e4sst sich eine diverse Gruppe von Analyten dank der ausgewogenen Retentionseigenschaften der Raptor Polar X S\u00e4ule in einem einzigen Lauf erfolgreich trennen.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0546.png\" alt=\"17 Polar Pesticide Panel on Raptor Polar X by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0546<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><th style=\"text-align: center;width: 75px\">Product Ion 3<\/th><th style=\"text-align: center;width: 75px\">Precursor 2<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">Aminomethylphosphonic acid (AMPA)<\/td><td class=\"oth\">0.805<\/td><td class=\"oth\">200<\/td><td class=\"oth\">110.1<\/td><td class=\"oth\">79.1<\/td><td class=\"oth\">63.1<\/td><td class=\"oth\">81.1<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\">Bialophos<\/td><td class=\"oth\">0.847<\/td><td class=\"oth\">100<\/td><td class=\"oth\">322.2<\/td><td class=\"oth\">88.2<\/td><td class=\"oth\">216.1<\/td><td class=\"oth\">134.2<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perchlorate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/14797-73-0\/Perchlorate\" target=\"_blank\" rel=\"noopener\">Perchlorate<\/a><\/td><td class=\"oth\">2.593<\/td><td class=\"oth\">5<\/td><td class=\"oth\">101.0<\/td><td class=\"oth\">84.95<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">98.9<\/td><td class=\"oth\">83<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glufosinate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/51276-47-2\/Glufosinate\" target=\"_blank\" rel=\"noopener\">Glufosinate<\/a><\/td><td class=\"oth\">3.376<\/td><td class=\"oth\">200<\/td><td class=\"oth\">180.2<\/td><td class=\"oth\">85.2<\/td><td class=\"oth\">95.1<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\">3-(Methylphophinico) propionic acid (MPPA)<\/td><td class=\"oth\">4.076<\/td><td class=\"oth\">100<\/td><td class=\"oth\">151.0<\/td><td class=\"oth\">63.0<\/td><td class=\"oth\">107.1<\/td><td class=\"oth\">133.2<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Trifluoracetic acid (TFA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/76-05-1\/Trifluoracetic acid\" target=\"_blank\" rel=\"noopener\">Trifluoracetic acid (TFA)<\/a><\/td><td class=\"oth\">4.423<\/td><td class=\"oth\">20<\/td><td class=\"oth\">113.0<\/td><td class=\"oth\">69.1<\/td><td class=\"oth\">19.1<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\">2-Hydroxyethane phosphonic acid (HEPA)<\/td><td class=\"oth\">4.969<\/td><td class=\"oth\">100<\/td><td class=\"oth\">125.1<\/td><td class=\"oth\">79.0<\/td><td class=\"oth\">95.0<\/td><td class=\"oth\">63<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Difluoroacetic acid (DFA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/381-73-7\/Difluoroacetic acid\" target=\"_blank\" rel=\"noopener\">Difluoroacetic acid (DFA)<\/a><\/td><td class=\"oth\">5.018<\/td><td class=\"oth\">200<\/td><td class=\"oth\">95.0<\/td><td class=\"oth\">51.1<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Chlorate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/14866-68-3\/Chlorate\" target=\"_blank\" rel=\"noopener\">Chlorate<\/a><\/td><td class=\"oth\">5.542<\/td><td class=\"oth\">100<\/td><td class=\"oth\">85.0<\/td><td class=\"oth\">69.0<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">83.0<\/td><td class=\"oth\">67.1<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ethephon\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/16672-87-0\/Ethephon\" target=\"_blank\" rel=\"noopener\">Ethephon<\/a><\/td><td class=\"oth\">5.564<\/td><td class=\"oth\">200<\/td><td class=\"oth\">143.1<\/td><td class=\"oth\">107.2<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\"><\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glyphosate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1071-83-6\/Glyphosate\" target=\"_blank\" rel=\"noopener\">Glyphosate<\/a><\/td><td class=\"oth\">6.113<\/td><td class=\"oth\">200<\/td><td class=\"oth\">168.1<\/td><td class=\"oth\">63.1<\/td><td class=\"oth\">79.1<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\">Bromide<\/td><td class=\"oth\">6.423<\/td><td class=\"oth\">2000<\/td><td class=\"oth\">80.9<\/td><td class=\"oth\">80.9<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">79.0<\/td><td class=\"oth\">79.0<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\">Bromate<\/td><td class=\"oth\">6.771<\/td><td class=\"oth\">600<\/td><td class=\"oth\">129.0<\/td><td class=\"oth\">113<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">127<\/td><td class=\"oth\">111.1<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\">N-acetyl AMPA<\/td><td class=\"oth\">6.932<\/td><td class=\"oth\">200<\/td><td class=\"oth\">152.1<\/td><td class=\"oth\">110.1<\/td><td class=\"oth\">62.9<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\">Fosetyl aluminum<\/td><td class=\"oth\">7.775<\/td><td class=\"oth\">80<\/td><td class=\"oth\">109.1<\/td><td class=\"oth\">81.0<\/td><td class=\"oth\">63.0<\/td><td class=\"oth\">78.9<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\">Phosphonic acid<\/td><td class=\"oth\">8.275<\/td><td class=\"oth\">500<\/td><td class=\"oth\">81.1<\/td><td class=\"oth\">62.9<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\">N-acetyl glufosinate<\/td><td class=\"oth\">9.980<\/td><td class=\"oth\">200<\/td><td class=\"oth\">222.2<\/td><td class=\"oth\">136.1<\/td><td class=\"oth\">134.1<\/td><td class=\"oth\">59.0<\/td><td class=\"oth\">&#8211;<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A32?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0546\" rel=\"noopener\">cat.# 9311A32<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>30 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>1 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water,  0.5% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile, 0.5% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>5.0<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>11.5<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>11.51<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>13<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0546.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<p>Zus\u00e4tzlich zu der von Restek entwickelten Methode, die in Abbildung 5 dargestellt ist, wurden die Raptor Polar X Analyse- und Vors\u00e4ulen unabh\u00e4ngig evaluiert und in die QuPPe-Methode selbst aufgenommen [1]. Sie werden in einer Methode f\u00fcr den Nachweis einer Vielzahl von polaren Pestiziden mittels LC-MS\/MS im negativen ESI-Modus verwendet.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Underivatisierte Aminos\u00e4uren<\/h2>\n\n\n\n<p>Aminos\u00e4uren sind eine diverse Gruppe hochpolarer Verbindungen, die \u00fcblicherweise mittels Reversed-Phase- oder Ionenaustausch-Chromatografie unter Verwendung von Vor- oder Nachs\u00e4ulenderivatisierung analysiert werden. Direkte Analyse von underivatisierten Aminos\u00e4uren ist aufgrund der geringen Retention und schlechten chromatografischen Performance schwierig. Auf einer Raptor Polar X S\u00e4ule werden underivatisierte Aminos\u00e4uren mit nichtpolaren, polaren, positiv oder negativ geladenen Seitenketten jedoch retardiert und lassen sich mit einer einzigen Methode leicht trennen. Abbildung 6 zeigt die Analyse von 21 Aminos\u00e4uren, darunter ein Taurin-Nahrungserg\u00e4nzungsmittel, in einer Matrix fl\u00fcssiger Babynahrung nach einfacher Proteinausf\u00e4llung und Verwendung des resultierenden Extrakts.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Abbildung 6:<\/strong>\u00a0Verbindungen mit unterschiedlichen Polarit\u00e4ten, wie diese Aminos\u00e4uren, k\u00f6nnen mit derselben Methode auf einer Raptor Polar X S\u00e4ule analysiert werden.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0579.png\" alt=\"Underivatized Amino Acids Analysis in Baby Formula on Raptor Polar X\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FF0579<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tryptophan\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/54-12-6\/Tryptophan\" target=\"_blank\" rel=\"noopener\">Tryptophan<\/a><\/td><td class=\"oth\">1.17<\/td><td class=\"oth\">205.07<\/td><td class=\"oth\">146.08<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Phenylalanine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/63-91-2\/Phenylalanine\" target=\"_blank\" rel=\"noopener\">Phenylalanine<\/a><\/td><td class=\"oth\">1.26<\/td><td class=\"oth\">166.13<\/td><td class=\"oth\">120.10<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Leucine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/61-90-5\/Leucine\" target=\"_blank\" rel=\"noopener\">Leucine<\/a><\/td><td class=\"oth\">1.41<\/td><td class=\"oth\">132.13<\/td><td class=\"oth\">86.10<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Isoleucine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/73-32-5\/Isoleucine\" target=\"_blank\" rel=\"noopener\">Isoleucine<\/a><\/td><td class=\"oth\">1.55<\/td><td class=\"oth\">132.13<\/td><td class=\"oth\">86.10<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Methionine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/59-51-8\/Methionine\" target=\"_blank\" rel=\"noopener\">Methionine<\/a><\/td><td class=\"oth\">1.62<\/td><td class=\"oth\">150.07<\/td><td class=\"oth\">104.10<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tyrosine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/556-03-6\/Tyrosine\" target=\"_blank\" rel=\"noopener\">Tyrosine<\/a><\/td><td class=\"oth\">1.69<\/td><td class=\"oth\">182.10<\/td><td class=\"oth\">136.08<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Taurine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/107-35-7\/Taurine\" target=\"_blank\" rel=\"noopener\">Taurine<\/a><\/td><td class=\"oth\">1.91<\/td><td class=\"oth\">126.07<\/td><td class=\"oth\">108.07<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Valine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/516-06-3\/Valine\" target=\"_blank\" rel=\"noopener\">Valine<\/a><\/td><td class=\"oth\">1.98<\/td><td class=\"oth\">118.13<\/td><td class=\"oth\">72.11<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Proline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/609-36-9\/Proline\" target=\"_blank\" rel=\"noopener\">Proline<\/a><\/td><td class=\"oth\">2.29<\/td><td class=\"oth\">116.13<\/td><td class=\"oth\">70.09<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Alanine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/302-72-7\/Alanine\" target=\"_blank\" rel=\"noopener\">Alanine<\/a><\/td><td class=\"oth\">3.00<\/td><td class=\"oth\">90.03<\/td><td class=\"oth\">44.10<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Threonine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/80-68-2\/Threonine\" target=\"_blank\" rel=\"noopener\">Threonine<\/a><\/td><td class=\"oth\">3.12<\/td><td class=\"oth\">120.13<\/td><td class=\"oth\">74.08<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glycine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56-40-6\/Glycine\" target=\"_blank\" rel=\"noopener\">Glycine<\/a><\/td><td class=\"oth\">3.62<\/td><td class=\"oth\">76.10<\/td><td class=\"oth\">30.11<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glutamine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56-85-9\/Glutamine\" target=\"_blank\" rel=\"noopener\">Glutamine<\/a><\/td><td class=\"oth\">3.87<\/td><td class=\"oth\">147.13<\/td><td class=\"oth\">84.07<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Serine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/302-84-1\/Serine\" target=\"_blank\" rel=\"noopener\">Serine<\/a><\/td><td class=\"oth\">3.93<\/td><td class=\"oth\">106.07<\/td><td class=\"oth\">60.09<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Asparagine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/70-47-3\/Asparagine\" target=\"_blank\" rel=\"noopener\">Asparagine<\/a><\/td><td class=\"oth\">4.08<\/td><td class=\"oth\">133.13<\/td><td class=\"oth\">74.07<\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Arginine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/74-79-3\/Arginine\" target=\"_blank\" rel=\"noopener\">Arginine<\/a><\/td><td class=\"oth\">4.47<\/td><td class=\"oth\">175.17<\/td><td class=\"oth\">70.09<\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Histidine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/71-00-1\/Histidine\" target=\"_blank\" rel=\"noopener\">Histidine<\/a><\/td><td class=\"oth\">4.66<\/td><td class=\"oth\">156.07<\/td><td class=\"oth\">110.16<\/td><\/tr>\n<tr><td class=\"num\">18.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Lysine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56-87-1\/Lysine\" target=\"_blank\" rel=\"noopener\">Lysine<\/a><\/td><td class=\"oth\">4.97<\/td><td class=\"oth\">147.13<\/td><td class=\"oth\">84.13<\/td><\/tr>\n<tr><td class=\"num\">19.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glutamic acid\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/617-65-2\/Glutamic acid\" target=\"_blank\" rel=\"noopener\">Glutamic acid<\/a><\/td><td class=\"oth\">5.89<\/td><td class=\"oth\">148.10<\/td><td class=\"oth\">84.10<\/td><\/tr>\n<tr><td class=\"num\">20.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cystine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56-89-3\/Cystine\" target=\"_blank\" rel=\"noopener\">Cystine<\/a><\/td><td class=\"oth\">6.10<\/td><td class=\"oth\">241.13<\/td><td class=\"oth\">152.00<\/td><\/tr>\n<tr><td class=\"num\">21.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Aspartic acid\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/617-45-8\/Aspartic acid\" target=\"_blank\" rel=\"noopener\">Aspartic acid<\/a><\/td><td class=\"oth\">7.12<\/td><td class=\"oth\">134.07<\/td><td class=\"oth\">74.06<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FF0579\" rel=\"noopener\">cat.# 9311A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>20:80 Water:acetonitrile, 0.01 N HCl<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>  Endogenous amino acids<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 0.5% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Mobile Phase B: 9:1 Acetonitrile:20 mM ammonium formate in water (pH 3.0) (The ammonium formate concentration is 20 mM relative to the total volume of mobile phase B. See preparation notes for instructions on diluting a 200 mM aqueous starting solution.) <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>12<\/td><td>88<\/td><\/tr><tr><td>3.50<\/td><td>0.5<\/td><td>12<\/td><td>88<\/td><\/tr><tr><td>8.00<\/td><td>0.5<\/td><td>70<\/td><td>30<\/td><\/tr><tr><td>8.01<\/td><td>0.5<\/td><td>12<\/td><td>88<\/td><\/tr><tr><td>10.0<\/td><td>0.5<\/td><td>12<\/td><td>88<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>A 200 \u00b5L aliquot of protein hydrolysate formula (Similac ALIMENTUM) was mixed with 800 \u00b5L of acetonitrile and 10 \u00b5L of 1 N HCl. After centrifugation at 4000 rpm for 5 minutes, the supernatant was diluted 20-fold with 20:80 water:acetonitrile (0.01 N HCl) and injected for analysis.<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td><i>Mobile Phase B Preparation:<\/i> To make 500 mL of mobile phase B, measure ~45 mL of water into a small beaker and add 1 mL of 10 M ammonium formate solution. Adjust pH to 3.0 by adding formic acid and then bring the volume to 50 mL with water. Combine this 50 mL ammonium formate solution (pH 3.0) with 450 mL of acetonitrile to complete the preparation.   <\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0579.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Ultrakurzkettige bis langkettige PFAS<\/h2>\n\n\n\n<p>Das abschlie\u00dfende Beispiel der F\u00e4higkeit der Raptor Polar X S\u00e4ule, die Produktivit\u00e4t einer Methode zu maximieren, ist eine Applikation, die den Weg f\u00fcr zuk\u00fcnftige Testverfahren ebnet. Aktuelle LC-MS\/MS-Methoden der Analyse per- und polyfluorierter Alkylverbindungen (PFAS) befassen sich in erster Linie mit kurzkettigen (C4-C6), langkettigen (C8 und h\u00f6her) sowie alternativen Verbindungen, ber\u00fccksichtigen jedoch nicht die neuen ultrakurzkettigen (C2 und C3) Verbindungen. Typische Reversed-Phase-Methoden f\u00fcr die PFAS-Analyse haben zu wenig Retention f\u00fcr ultrakurzkettige PFAS, w\u00e4hrend andere, auf Ionenaustausch basierende Methoden, zu viel Retention aufweisen, was eine schlechte chromatografische Performance zur Folge hat. Mit der Raptor Polar X S\u00e4ule lassen sich aufgrund des ausgewogenen Retentionsverhaltens sowohl ultrakurzkettige als auch langkettige PFAS in einem einzigen isokratischen HPLC-Lauf innerhalb von 5 Minuten analysieren (Abbildung 7).<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Abbildung 7:<\/strong>\u00a0Ultrakurzkettige, traditionelle und alternative PFAS lassen sich mit einer einzigen Methode auf der neuartigen station\u00e4ren Phase Raptor Polar X analysieren.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0569.png\" alt=\"Ultrashort- Through Long-Chain and Alternative PFAS on Raptor Polar X\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EV0569<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/L)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">11-Chloroeicosafluoro-3-oxanonane-1-sulfonate (11CL-PF3OUdS)<\/td><td class=\"oth\">1.25<\/td><td class=\"oth\">400<\/td><td class=\"oth\">630.78<\/td><td class=\"oth\">450.80<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\">9-Chlorohexadecafluoro-3-oxanonane-1-sulfonate (9Cl-PF3ONS)<\/td><td class=\"oth\">1.34<\/td><td class=\"oth\">400<\/td><td class=\"oth\">530.78<\/td><td class=\"oth\">350.85<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorooctanesulfonic acid (PFOS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1763-23-1\/Perfluorooctanesulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorooctanesulfonic acid (PFOS)<\/a><\/td><td class=\"oth\">1.38<\/td><td class=\"oth\">400<\/td><td class=\"oth\">498.84<\/td><td class=\"oth\">79.97<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorohexanesulfonic acid (PFHxS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/355-46-4\/Perfluorohexanesulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorohexanesulfonic acid (PFHxS)<\/a><\/td><td class=\"oth\">1.49<\/td><td class=\"oth\">400<\/td><td class=\"oth\">398.90<\/td><td class=\"oth\">79.97<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorobutanesulfonic acid (PFBS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/375-73-5\/Perfluorobutanesulfonic acid\" target=\"_blank\" rel=\"noopener\">Perfluorobutanesulfonic acid (PFBS)<\/a><\/td><td class=\"oth\">1.64<\/td><td class=\"oth\">400<\/td><td class=\"oth\">298.97<\/td><td class=\"oth\">79.97<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\">Perfluoropropanesulfonic acid (PFPrS)<\/td><td class=\"oth\">1.73<\/td><td class=\"oth\">400<\/td><td class=\"oth\">248.97<\/td><td class=\"oth\">79.98<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\">Perfluoroethanesulfonic acid (PFEtS)<\/td><td class=\"oth\">1.86<\/td><td class=\"oth\">400<\/td><td class=\"oth\">198.98<\/td><td class=\"oth\">79.92<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/L)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Hexafluoropropylene oxide dimer acid (HFPO-DA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/13252-13-6\/Hexafluoropropylene oxide dimer acid\" target=\"_blank\" rel=\"noopener\">Hexafluoropropylene oxide dimer acid (HFPO-DA)<\/a><\/td><td class=\"oth\">2.06<\/td><td class=\"oth\">400<\/td><td class=\"oth\">284.97<\/td><td class=\"oth\">168.92<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorooctanoic acid (PFOA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/335-67-1\/Perfluorooctanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorooctanoic acid (PFOA)<\/a><\/td><td class=\"oth\">2.11<\/td><td class=\"oth\">400<\/td><td class=\"oth\">412.90<\/td><td class=\"oth\">368.91<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ammonium 4,8-dioxa-3H-perfluorononanoate (ADONA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/919005-14-4\/Ammonium 4,8-dioxa-3H-perfluorononanoate\" target=\"_blank\" rel=\"noopener\">Ammonium 4,8-dioxa-3H-perfluorononanoate (ADONA)<\/a><\/td><td class=\"oth\">2.15<\/td><td class=\"oth\">400<\/td><td class=\"oth\">376.90<\/td><td class=\"oth\">250.93<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorohexanoic acid (PFHxA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/307-24-4\/Perfluorohexanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorohexanoic acid (PFHxA)<\/a><\/td><td class=\"oth\">2.36<\/td><td class=\"oth\">400<\/td><td class=\"oth\">312.97<\/td><td class=\"oth\">268.90<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perfluorobutanoic acid (PFBA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/375-22-4\/Perfluorobutanoic acid\" target=\"_blank\" rel=\"noopener\">Perfluorobutanoic acid (PFBA)<\/a><\/td><td class=\"oth\">2.76<\/td><td class=\"oth\">400<\/td><td class=\"oth\">212.97<\/td><td class=\"oth\">168.97<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\">Perfluoropropionic acid (PFPrA)<\/td><td class=\"oth\">3.06<\/td><td class=\"oth\">400<\/td><td class=\"oth\">163.03<\/td><td class=\"oth\">119.01<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Trifluoroacetic acid (TFA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/76-05-1\/Trifluoroacetic acid\" target=\"_blank\" rel=\"noopener\">Trifluoroacetic acid (TFA)<\/a><\/td><td class=\"oth\">3.77<\/td><td class=\"oth\">400<\/td><td class=\"oth\">113.03<\/td><td class=\"oth\">69.01<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A52?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0569\" rel=\"noopener\">cat.# 9311A52<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>50:50 Water:methanol<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>400  ng\/L<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 10 mM ammonium formate, 0.05% formic acid   <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>60:40 Acetonitrile:methanol, 0.05% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>15<\/td><td>85<\/td><\/tr><tr><td>8.00<\/td><td>0.5<\/td><td>15<\/td><td>85<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0569.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Passivierung \u2013 Ja oder nein?<\/h2>\n\n\n\n<p>Die Analyse von Glyphosat im Spurenbereich kann sich schwierig gestalten, da es mit aktiven Metallzentren in einem LC-System Chelate bilden kann. Obwohl die Raptor Polar X S\u00e4ule einen behandelten Probenflussweg besitzt und gebrauchs\u00acfertig geliefert wird, kann eine Passivierung der Metallkomponenten im Proben\u00acflussweg Ihres LC-Ger\u00e4ts hilfreich sein. Ob eine Passivierung erforderlich ist oder nicht, h\u00e4ngt von der jeweiligen Applikation und Ihrer spezifischen Ger\u00e4tekon-figuration ab. F\u00fcr die Analyse von polaren Verbindungen, die bekannterma\u00dfen Chelate bilden, wie z. B. Glyphosat, empfiehlt Restek eine Behandlung Ihres Systems mit LC-Passivierungsl\u00f6sung (Art.-Nr. 32475) vor dem Probenlauf.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Eine neuartige station\u00e4re Phase basierend auf der bew\u00e4hrten Raptor Qualit\u00e4t<\/h2>\n\n\n\n<p>Raptor Polar X wurde nach denselben anspruchsvollen Vorgaben entwickelt, gefertigt und gepr\u00fcft, die Raptor S\u00e4ulen zu einem Synonym f\u00fcr Qualit\u00e4t gemacht haben. Egal ob es um die robuste Performance von Injektion-zu-Injektion bei der Analyse von reellen Proben in schwierigen Matrizes wie Spinat (Abbildung 8) oder um die zuverl\u00e4ssige Leistungsf\u00e4higkeit von S\u00e4ule-zu-S\u00e4ule bis zum letztendlichen Austausch der S\u00e4ule (Abbildung 9) geht \u2013 Sie k\u00f6nnen sich auf die Qualit\u00e4t der Marke Raptor verlassen. Au\u00dferdem gew\u00e4hrleistet die Funktionalit\u00e4t der Hybridretention der Raptor Polar X S\u00e4ule unter Verwendung eines einzigen Liganden die gleichbleibende Fertigungsqualit\u00e4t aller S\u00e4ulen, die sogar aggressiven Bedingungen wie Injektionen gro\u00dfer Wasservolumina ohne Verlust der station\u00e4ren Phase standhalten k\u00f6nnen.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Abbildung 8:<\/strong>\u00a0Sehr gute Peakformen und hervorragende Stabilit\u00e4t der Retentionszeiten nach hunderten von Injektionen eines Spinatextrakts veranschaulichen die robuste Performance der Raptor Polar X S\u00e4ulen f\u00fcr die Analyse polarer Verbindungen.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0547.png\" alt=\"Polar Pesticides in Spinach on Raptor Polar X by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0547<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><th style=\"text-align: center;width: 75px\">Product Ion 3<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">AMPA<\/td><td class=\"oth\">100<\/td><td class=\"oth\">110.10<\/td><td class=\"oth\">79.05<\/td><td class=\"oth\">63.10<\/td><td class=\"oth\">81.10<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glufosinate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/51276-47-2\/Glufosinate\" target=\"_blank\" rel=\"noopener\">Glufosinate<\/a><\/td><td class=\"oth\">100<\/td><td class=\"oth\">180.20<\/td><td class=\"oth\">85.15<\/td><td class=\"oth\">95.10<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glyphosate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1071-83-6\/Glyphosate\" target=\"_blank\" rel=\"noopener\">Glyphosate<\/a><\/td><td class=\"oth\">100<\/td><td class=\"oth\">168.10<\/td><td class=\"oth\">63.05<\/td><td class=\"oth\">79.05<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A32?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0547\" rel=\"noopener\">cat.# 9311A32<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>30 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Raptor Polar X guard column cartridge 5 mm, 2.1 mm ID, 2.7 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A0252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0547\" rel=\"noopener\">cat.# 9311A0252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Spinach extract<\/td><\/tr>\n<td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water,  0.5% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile, 0.5% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>5.0<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>6.5<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>6.51<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>8<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>Frozen spinach was added to a Blixer processor with dry ice (3:1-4:1 ratio) and then ground into a very fine powder. The homogenate was placed into the freezer immediately. A 5.0 gram sample of the spinach powder was weighed into a 50 mL centrifuge tube (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/25846?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0547\" rel=\"noopener\">cat.# 25846<\/a>). According to the QuPPe method (Quick Polar Pesticides Method), 5.0 mL of methanol with 1.0% formic acid was added into the centrifuge tube. The tube was shaken by hand for 1 min and then by a mechanical shaker vigorously for 5 min. After centrifuging for 10 min at 4200 rpm, the supernatant was filtered through a 0.22 \u03bcm filter (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/23984?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0547\" rel=\"noopener\">cat.# 23984<\/a>). The final extract was fortified with the AMPA, glufosinate, and glyphosate at a final concentration of 100 ng\/mL.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0547.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Abbildung 9:<\/strong>\u00a0Raptor Polar X S\u00e4ulen gew\u00e4hrleisten zuverl\u00e4ssige Reproduzierbarkeit von Lot zu Lot.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0543.png\" alt=\"Lot-to-Lot Comparison: Polar Pesticides in Solvent on Raptor Polar X by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0543<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><th style=\"text-align: center;width: 75px\">Product Ion 3<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">AMPA<\/td><td class=\"oth\">100<\/td><td class=\"oth\">110.10<\/td><td class=\"oth\">79.05<\/td><td class=\"oth\">63.10<\/td><td class=\"oth\">81.10<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glufosinate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/51276-47-2\/Glufosinate\" target=\"_blank\" rel=\"noopener\">Glufosinate<\/a><\/td><td class=\"oth\">100<\/td><td class=\"oth\">180.20<\/td><td class=\"oth\">85.15<\/td><td class=\"oth\">95.10<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glyphosate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1071-83-6\/Glyphosate\" target=\"_blank\" rel=\"noopener\">Glyphosate<\/a><\/td><td class=\"oth\">100<\/td><td class=\"oth\">168.10<\/td><td class=\"oth\">63.05<\/td><td class=\"oth\">79.05<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A32?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0543\" rel=\"noopener\">cat.# 9311A32<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>30 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water,  0.5% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile, 0.5% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>5.0<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>6.5<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>6.51<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>8<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0543.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Raptor Polar X S\u00e4ulen:&nbsp;Revolutionierung der LC-Analyse polarer Verbindungen<\/h2>\n\n\n\n<p>Geringe Retention, schwache Response, komplexe Verfahren zur Probenvorbereitung &#8230; die Analyse polarer Verbindungen war in der Vergangenheit oft mit gro\u00dfen Problemen bez\u00fcglich Datenqualit\u00e4t und Laborproduktivit\u00e4t behaftet. Jetzt l\u00e4sst sich die Analyse dieser anspruchsvollen und vielf\u00e4ltigen Verbindungen mithilfe der Raptor Polar X S\u00e4ulen mit ihrer neuartigen Phasenchemie wesentlich einfacher durchf\u00fchren, indem die Retention per HILIC und Ionenaustausch an einem einzigen Liganden kombiniert wird. Durch Bindung dieses speziellen Liganden an Core-Shell-Partikel (SPP) hat Restek eine Phase geschaffen, die eine Vielzahl polarer Verbindungen retardiert und effizient trennt. Die durch diese Merkmale erzeugte Synergie bietet die beispiellose F\u00e4higkeit zur Analyse einer Vielzahl polarer Verbindungen und stellt LC-Analytikern in vielen Industriezweigen ein leistungsstarkes, produktives und flexibles Werkzeug zur Verf\u00fcgung.<\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Literatur<\/h2>\n\n\n\n<ol class=\"wp-block-list\">\n<li>M. Anastassiades, A.-K. Wachtler, D. I. Kolberg, E. Eichhorn, H. Marks, A. Benkenstein, S. Zechmann; D. Mack, C. Wildgrube, A. Barth, I. Sigalov, S. G\u00f6rlich, D. D\u00f6rk, G. Cerchia, Quick method for the analysis of highly polar pesticides in food involving extraction with acidified methanol and LC &#8211; or ICMS\/MS Measurement &#8211; I. Food of Plant Origin (QuPPe-PO-Method)\u2013Version 12 (published on EURL-SRM website on July 23, 2021).&nbsp;<a href=\"https:\/\/www.eurl-pesticides.eu\/docs\/public\/tmplt_article.asp?LabID=200&amp;CntID=1115&amp;Theme_ID=1&amp;Pdf=False&amp;Lang=EN\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.eurl-pesticides.eu\/docs\/public\/tmplt_article.asp?LabID=200&amp;CntID=1115&amp;Theme_ID=1&amp;Pdf=False&amp;Lang=EN<\/a><\/li>\n<\/ol>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Die einzigartige Hybridphase in den Raptor Polar X-S\u00e4ulen sorgt f\u00fcr ein Gleichgewicht zwischen HILIC- und Ionenaustausch-Retentionsmodi und ist damit ideal f\u00fcr die Analyse eines breiten Spektrums polarer Verbindungen, insbesondere in Verbindung mit Massenspektrometrie.<\/p>\n","protected":false},"author":11,"featured_media":7420,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[787],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[2481,2499],"resource-technique":[2326,2355,2363],"ppma_author":[414],"class_list":["post-43834","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-artikel","product-library-flussigchromatographie-produkte","product-library-lc-saulen","resource-technique-flussigchromatografie","resource-technique-hilic-de","resource-technique-ms-ms-de"],"acf":[],"taxonomy_info":{"category":[{"value":787,"label":"Artikel"}],"product-library":[{"value":2481,"label":"Fl\u00fcssigchromatographie Produkte"},{"value":2499,"label":"LC S\u00e4ulen"}],"resource-technique":[{"value":2326,"label":"Fl\u00fcssigchromatografie"},{"value":2355,"label":"HILIC"},{"value":2363,"label":"MS\/MS"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-GNSS3195-1024x536.jpg",1024,536,true],"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/de\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":787,"name":"Artikel","slug":"artikel","term_group":0,"term_taxonomy_id":787,"taxonomy":"category","description":"","parent":0,"count":469,"filter":"raw","cat_ID":787,"category_count":469,"category_description":"","cat_name":"Artikel","category_nicename":"artikel","category_parent":0}],"tag_info":false,"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/43834","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/comments?post=43834"}],"version-history":[{"count":19,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/43834\/revisions"}],"predecessor-version":[{"id":85809,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/43834\/revisions\/85809"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/media\/7420"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/media?parent=43834"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/categories?post=43834"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/tags?post=43834"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/industries-application?post=43834"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/post-badge?post=43834"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-type?post=43834"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/product-library?post=43834"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-technique?post=43834"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/ppma_author?post=43834"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}