{"id":48497,"date":"2018-01-16T13:30:00","date_gmt":"2018-01-16T13:30:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/can-i-use-a-100-aqueous-mobile-phase-with-my-lc-column\/"},"modified":"2025-11-26T17:48:41","modified_gmt":"2025-11-26T17:48:41","slug":"can-i-use-a-100-aqueous-mobile-phase-with-my-lc-column","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/de\/blogs\/gnbl4597\/can-i-use-a-100-aqueous-mobile-phase-with-my-lc-column","title":{"rendered":"Can I use a 100% aqueous mobile phase with my LC column?"},"content":{"rendered":"\n<p>You might be asking this because you have read that not all columns are OK to use with highly aqueous content (&gt;95%) in the mobile phase.&nbsp; Or maybe the caption caught your eye because you have recently encountered some difficulty with using highly aqueous mobile phase.<\/p>\n\n\n\n<p>There is a phenomenon called \u201cphase dewetting\u201d that can occur with LC phases that are composed of the longer alkyl chains such as C18 and C8.&nbsp; Phase dewetting results in a sudden and dramatic loss of retention, as well as poor peak shape. If you would like to read more about phase dewetting, I suggest reading the following:<\/p>\n\n\n\n<figure class=\"wp-block-embed is-type-wp-embed is-provider-wordpress-embed wp-block-embed-wordpress-embed\"><div class=\"wp-block-embed__wrapper\">\n<blockquote class=\"wp-embedded-content\" data-secret=\"6tqleUZMWc\"><a href=\"https:\/\/discover.restek.com\/gnbl4567\/a-c18-is-a-c18-right\/\">A C18 is a C18, right?<\/a><\/blockquote><iframe loading=\"lazy\" class=\"wp-embedded-content\" sandbox=\"allow-scripts\" security=\"restricted\" style=\"position: absolute; visibility: hidden;\" title=\"&#8220;A C18 is a C18, right?&#8221; &#8212; \" src=\"https:\/\/discover.restek.com\/gnbl4567\/a-c18-is-a-c18-right\/embed\/#?secret=DB6MZdhwOf#?secret=6tqleUZMWc\" data-secret=\"6tqleUZMWc\" width=\"600\" height=\"338\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\"><\/iframe>\n<\/div><\/figure>\n\n\n\n<p><a href=\"http:\/\/www.chromatographyonline.com\/lcgc-blog-chromatography-technical-tips-polar-analyte-retention-and-phase-collapse\" target=\"_blank\" rel=\"noreferrer noopener\">http:\/\/www.chromatographyonline.com\/lcgc-blog-chromatography-technical-tips-polar-analyte-retention-and-phase-collapse<\/a><\/p>\n\n\n\n<p><a href=\"http:\/\/files.pharmtech.com\/alfresco_images\/pharma\/2014\/08\/22\/55290841-3e3e-433b-a5d4-adb7f6495a06\/article-32973.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">http:\/\/files.pharmtech.com\/alfresco_images\/pharma\/2014\/08\/22\/55290841-3e3e-433b-a5d4-adb7f6495a06\/article-32973.pdf<\/a><\/p>\n\n\n\n<p><a href=\"http:\/\/www.waters.com\/webassets\/cms\/promotion\/docs\/Polar-Retention-Reversed-Phase-LC-Webinar-%20Pres-Kevin-Jenkins-Oct2016.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">http:\/\/www.waters.com\/webassets\/cms\/promotion\/docs\/Polar-Retention-Reversed-Phase-LC-Webinar-%20Pres-Kevin-Jenkins-Oct2016.pdf<\/a><\/p>\n\n\n\n<p>As mentioned in one of the above listed blog posts, \u201cA C18 is a C18, right?, our \u201cAqueous C18\u201d phases are quite different from conventional C18 phases. &nbsp;This includes our Ultra Aqueous C18 and Pinnace DB Aqueous C18 phases.&nbsp; We also have a few other phases that can be used with up to 100% aqueous mobile phase. You might notice from reading the referenced articles that pore size and bonding density has some impact also. Here is an all- inclusive list of Restek LC column phases that will tolerate 100% aqueous conditions:<\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1801\" data-type=\"link\" data-id=\"https:\/\/www.restek.com\/c\/1801\" target=\"_blank\" rel=\"noopener\">Ultra Aqueous C18<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1810\" data-type=\"link\" data-id=\"https:\/\/www.restek.com\/c\/1810\" target=\"_blank\" rel=\"noopener\">Ultra IBD<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1803\" target=\"_blank\" rel=\"noopener\">Ultra Biphenyl<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1811\" target=\"_blank\" rel=\"noopener\">Ultra PFP Propyl<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1802\" target=\"_blank\" rel=\"noopener\">Ultra Aromax<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1809\" target=\"_blank\" rel=\"noopener\">Ultra Cyano<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1804\" data-type=\"link\" data-id=\"https:\/\/www.restek.com\/c\/1804\" target=\"_blank\" rel=\"noopener\">Ultra C1<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1778\" target=\"_blank\" rel=\"noopener\">Pinnacle DB Aqueous C18<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1783\" target=\"_blank\" rel=\"noopener\">Pinnacle DB IBD<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1779\" target=\"_blank\" rel=\"noopener\">Pinnacle DB Biphenyl<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1784\" target=\"_blank\" rel=\"noopener\">Pinnacle DB PFP Propyl<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1782\" target=\"_blank\" rel=\"noopener\">Pinnacle DB Cyano<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1816\" target=\"_blank\" rel=\"noopener\">Viva C4<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1814\" target=\"_blank\" rel=\"noopener\">Viva Biphenyl<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1818\" target=\"_blank\" rel=\"noopener\">Viva PFP Propyl<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1798\" target=\"_blank\" rel=\"noopener\">ROC Phenyl-Hexyl<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1797\" target=\"_blank\" rel=\"noopener\">Roc Cyano<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1788\" target=\"_blank\" rel=\"noopener\">Raptor Biphenyl<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1791\" target=\"_blank\" rel=\"noopener\">Raptor EtG\/EtS<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1792\" target=\"_blank\" rel=\"noopener\">Raptor Fluorophenyl<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1776\" target=\"_blank\" rel=\"noopener\">Force Fluorophenyl<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.restek.com\/c\/1774\" target=\"_blank\" rel=\"noopener\">Force Biphenyl<\/a><\/p>\n\n\n\n<p>Please note that although the above phases are known to be aqueous tolerant, we may not have generated data (chromatograms or application notes) at &gt;95% water content in the mobile phase. This is simply because we have not performed the application and, most likely, there has not been much demand for using a high aqueous mobile phase for applications that are commonly run on that column phase.&nbsp; If you do have any questions about whether or not your column is suitable for an application, please feel free to contact us in&nbsp;Tech Service.<\/p>\n\n\n\n<p>I hope you found this post helpful.<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>You might be asking this because you have read that not all columns are OK to use with highly aqueous content (&gt;95%) in the mobile phase. Or maybe the caption caught your eye because you have recently encountered some difficulty&#8230;<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[9],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[2326],"ppma_author":[602],"class_list":["post-48497","post","type-post","status-publish","format-standard","hentry","category-blogs","resource-technique-flussigchromatografie"],"acf":[],"taxonomy_info":{"category":[{"value":9,"label":"Blogs"}],"resource-technique":[{"value":2326,"label":"Fl\u00fcssigchromatografie"}]},"featured_image_src_large":false,"author_info":{"display_name":"DRCadmin","author_link":"https:\/\/discover.restek.com\/de\/author\/drcadmin\/"},"comment_info":0,"category_info":[{"term_id":9,"name":"Blogs","slug":"blogs","term_group":0,"term_taxonomy_id":9,"taxonomy":"category","description":"","parent":0,"count":444,"filter":"raw","cat_ID":9,"category_count":444,"category_description":"","cat_name":"Blogs","category_nicename":"blogs","category_parent":0}],"tag_info":false,"authors":[{"term_id":602,"user_id":0,"is_guest":1,"slug":"nancy-schwartz","display_name":"Nancy Schwartz","avatar_url":"https:\/\/secure.gravatar.com\/avatar\/?s=96&d=mm&r=g","0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/48497","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/comments?post=48497"}],"version-history":[{"count":5,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/48497\/revisions"}],"predecessor-version":[{"id":65330,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/48497\/revisions\/65330"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/media?parent=48497"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/categories?post=48497"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/tags?post=48497"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/industries-application?post=48497"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/post-badge?post=48497"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-type?post=48497"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/product-library?post=48497"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-technique?post=48497"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/ppma_author?post=48497"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}