{"id":48542,"date":"2023-11-28T00:00:00","date_gmt":"2023-11-28T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/kann-soll-man-hplc-saulen-entgegen-der-empfohlenen-flussrichtung-spulen-regenerieren\/"},"modified":"2026-01-28T22:25:21","modified_gmt":"2026-01-28T22:25:21","slug":"kann-soll-man-hplc-saulen-entgegen-der-empfohlenen-flussrichtung-spulen-regenerieren","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/de\/blogs-de\/gnbl4635-de\/kann-soll-man-hplc-saulen-entgegen-der-empfohlenen-flussrichtung-spulen-regenerieren","title":{"rendered":"Kann \/ soll man HPLC-S\u00e4ulen entgegen der empfohlenen Flussrichtung sp\u00fclen \/ regenerieren?"},"content":{"rendered":"\n<p>Es kann schon sinnvoll sein, eine verschmutzte HPLC-S\u00e4ule umgedreht zu sp\u00fclen.<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Der meiste Schmutz lagert sich am S\u00e4ulenanfang ab. Dieser Schmutz kann am schnellsten beim Sp\u00fclen entgegen der normalen Flussrichtung entfernt werden, da er dann nicht \u00fcber die ganze S\u00e4ulenl\u00e4nge zum S\u00e4ulenausgang transportiert werden muss.<\/li>\n\n\n\n<li>Au\u00dferdem kann die Umkehr der Flussrichtung dabei helfen, ein Totvolumen am S\u00e4ulenkopf &#8211; zumindest zeitweise &#8211; zu \u201ereparieren\u201c.<\/li>\n<\/ul>\n\n\n\n<p>ABER: Nicht jede S\u00e4ule kann problemlos umgedreht betrieben werden!<\/p>\n\n\n\n<p>Meist ist es kein Problem bei den klassischen 3 und 5\u03bcm Kieselgels\u00e4ulen.<\/p>\n\n\n\n<p>Aber sobald die S\u00e4ulen irgendwelche Besonderheiten aufweisen, ist Vorsicht angebracht, um auch langfristig eine gleichbleibende Trennleistung sicherzustellen.<\/p>\n\n\n\n<p>Es gibt diverse Gr\u00fcnde (Herstellung, Hardware etc.), weshalb LC-S\u00e4ulen nicht entgegen der empfohlenen Flussrichtung betrieben werden k\u00f6nnen oder sollen.<\/p>\n\n\n\n<p>Deshalb sicherheitshalber immer vorher beim Hersteller nachfragen, ob die S\u00e4ule daf\u00fcr geeignet ist!<\/p>\n\n\n\n<p>Und nicht vergessen: Beim Sp\u00fclen von S\u00e4ulen &#8211; egal in welcher Richtung &#8211;<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>immer zuerst die Vors\u00e4ule entfernen<\/li>\n\n\n\n<li>den S\u00e4ulenausgang in ein Abfallgef\u00e4\u00df leiten<\/li>\n\n\n\n<li>zun\u00e4chst nur mit halber Flussrate sp\u00fclen, um pl\u00f6tzlichen starken Druckanstieg zu vermeiden<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>In this post, we discuss whether you could or should reverse the flow on your LC column to help regenerate it.<\/p>\n","protected":false},"author":58,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[792],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[2326],"ppma_author":[613],"class_list":["post-48542","post","type-post","status-publish","format-standard","hentry","category-blogs-de","resource-technique-flussigchromatografie"],"acf":[],"taxonomy_info":{"category":[{"value":792,"label":"Blogs"}],"resource-technique":[{"value":2326,"label":"Fl\u00fcssigchromatografie"}]},"featured_image_src_large":false,"author_info":{"display_name":"Ute Beyer","author_link":"https:\/\/discover.restek.com\/de\/author\/ute-beyer\/"},"comment_info":0,"category_info":[{"term_id":792,"name":"Blogs","slug":"blogs-de","term_group":0,"term_taxonomy_id":792,"taxonomy":"category","description":"","parent":0,"count":438,"filter":"raw","cat_ID":792,"category_count":438,"category_description":"","cat_name":"Blogs","category_nicename":"blogs-de","category_parent":0}],"tag_info":false,"authors":[{"term_id":613,"user_id":58,"is_guest":0,"slug":"ute-beyer","display_name":"Ute Beyer","avatar_url":"https:\/\/secure.gravatar.com\/avatar\/?s=96&d=mm&r=g","0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/48542","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/users\/58"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/comments?post=48542"}],"version-history":[{"count":3,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/48542\/revisions"}],"predecessor-version":[{"id":85792,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/48542\/revisions\/85792"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/media?parent=48542"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/categories?post=48542"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/tags?post=48542"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/industries-application?post=48542"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/post-badge?post=48542"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-type?post=48542"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/product-library?post=48542"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-technique?post=48542"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/ppma_author?post=48542"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}