{"id":51640,"date":"2021-11-11T13:30:00","date_gmt":"2021-11-11T13:30:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/chiral-separations-5-separation-with-rt-bdexsa-rt-bdexcst-and-rt-dexsa\/"},"modified":"2026-01-28T15:50:53","modified_gmt":"2026-01-28T15:50:53","slug":"chiral-separations-5-separation-with-rt-bdexsa-rt-bdexcst-and-rt-dexsa","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/de\/blogs\/gnbl4622\/chiral-separations-5-separation-with-rt-bdexsa-rt-bdexcst-and-rt-dexsa","title":{"rendered":"Chiral Separations 5: Separation with Rt-\u03b2DEXsa, Rt-\u03b2DEXcst and Rt-\u03b3DEXsa"},"content":{"rendered":"\n

In this blog we\u2019ll continue looking into individual columns and specific separations. We\u2019ll focus on Rt-\u03b2DEXsa, Rt-\u03b2DEXcst and Rt-\u03b3DEXsa (Table 1), which all have somewhat different selectivity from the columns discussed in the last blog (Rt-\u03b2DEXm, Rt-\u03b2DEXsm and Rt-\u03b2DEXse).<\/p>\n\n\n\n

Table 1: Resolution of selected chiral compounds by Rt-\u03b2DEXsa, Rt-\u03b2DEXcst and Rt-\u03b3DEXsa<\/h5>\n\n\n\n
 <\/td>Rt-\u00dfDEXsa<\/strong><\/td>Rt-\u00dfDEXcst<\/strong><\/td>Rt-<\/strong>\u03b3DEXsa<\/strong><\/td><\/tr>
1-octen-3-ol<\/strong><\/td>2.00<\/td>ns<\/td>1.35<\/td><\/tr>
\u03b2-citronellol<\/strong><\/td>0.98<\/td>ns<\/td>ns<\/td><\/tr>
menthol<\/strong><\/td>0.93<\/td>0.89<\/td>2.89<\/td><\/tr>
camphor<\/strong><\/td>4.20<\/td>2.22t<\/td>3.64<\/td><\/tr>
\u03b1-ionone<\/strong><\/td>4.69<\/td>1.37<\/td>8.43<\/td><\/tr>
\u03b3-nonalactone<\/strong><\/td>4.00<\/td>3.82<\/td>0.99<\/td><\/tr>
\u03b3-undecalactone<\/strong><\/td>3.65<\/td>3.18<\/td>0.84<\/td><\/tr>
\u03b4-dodecalactone<\/strong><\/td>1.91<\/td>1.69<\/td>0.75<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Chiral Separations with Rt-\u03b2DEXsa<\/h2>\n\n\n\n

The Rt-bDEXsa has a significantly different selectivity than the other chiral columns. It provides the best separation of 1-octen-3-ol, carvone, camphor, b-citronellol (Figure 1A-D, respectively), and rose oxides (Figure 2).<\/p>\n\n\n\n

\"shape\"<\/figure>\n\n\n\n

Figure 1: Separation of A) 1-octen-3-ol, B) \u03b2-citronellol, C) carvone and D) Camphor on Rt-bDEXsa<\/p>\n\n\n\n

\"lighting\"<\/figure>\n\n\n\n

Figure 2: Separation of Rose oxides on Rt-bDEXsa<\/p>\n\n\n\n

Chiral Separation with Rt-\u00dfDEXcst<\/h2>\n\n\n\n

This column is optimum for semivolatile chiral compounds. It provides good resolution of the irone isomers (found in iris flowers; Figure 3). This is also suitable column for resolution of the \u03b3- and \u03b4-lactones (Figure 4).<\/p>\n\n\n\n

\"shape\"<\/figure>\n\n\n\n

Figure 3: Separation of irone isomers on Rt-\u00dfDEXcst<\/p>\n\n\n\n

\"chart,<\/figure>\n\n\n\n

Figure 4: Separation of lactones on Rt-\u00dfDEXcst<\/p>\n\n\n\n

Chiral Separations with Rt-\u03b3DEXsa<\/h2>\n\n\n\n

The Rt-\u03b3DEXsa is unique in the Restek chiral column selection due to doping of \u03b3-cyclodextrins into the stationary phase polymer. The \u03b3-cyclodextrins have a larger cavity; therefore, this column is useful for larger organic molecules. While our study was focused more on small molecules, the Rt-\u03b3DEXsa had very good separation for menthol and \u03b1-ionone (Figure 5).<\/p>\n\n\n\n

\"shape\"<\/figure>\n\n\n\n

Figure 5: Separation of A) menthol and B) \u03b1-Ionone on Rt- \u03b3DEXsa<\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

In this blog we’ll continue looking into individual columns and specific separations. We’ll focus on Rt-βDEXsa, Rt-βDEXcst and Rt-γDEXsa (Table 1), which all have somewhat different selectivity from the columns discussed in the last blog (Rt-βDEXm, Rt-βDEXsm and Rt-βDEXse).<\/p>\n","protected":false},"author":50,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[9],"tags":[],"industries-application":[2290],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[2332],"ppma_author":[591],"class_list":["post-51640","post","type-post","status-publish","format-standard","hentry","category-blogs","industries-application-food-beverage-it","resource-technique-gascromatografia-gc"],"acf":[],"taxonomy_info":{"category":[{"value":9,"label":"Blogs"}],"industries-application":[{"value":2290,"label":"Food & Beverage"}],"resource-technique":[{"value":2332,"label":"Gascromatografia (GC)"}]},"featured_image_src_large":false,"author_info":{"display_name":"Jana Hepner, PhD","author_link":"https:\/\/discover.restek.com\/de\/author\/jana-hepner-phd\/"},"comment_info":0,"category_info":[{"term_id":9,"name":"Blogs","slug":"blogs","term_group":0,"term_taxonomy_id":9,"taxonomy":"category","description":"","parent":0,"count":438,"filter":"raw","cat_ID":9,"category_count":438,"category_description":"","cat_name":"Blogs","category_nicename":"blogs","category_parent":0}],"tag_info":false,"authors":[{"term_id":591,"user_id":50,"is_guest":0,"slug":"jana-hepner-phd","display_name":"Jana Hepner, PhD","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-hepner-jana.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-hepner-jana.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/51640","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/users\/50"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/comments?post=51640"}],"version-history":[{"count":3,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/51640\/revisions"}],"predecessor-version":[{"id":85257,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/51640\/revisions\/85257"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/media?parent=51640"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/categories?post=51640"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/tags?post=51640"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/industries-application?post=51640"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/post-badge?post=51640"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-type?post=51640"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/product-library?post=51640"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-technique?post=51640"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/ppma_author?post=51640"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}