{"id":51821,"date":"2025-02-18T00:00:00","date_gmt":"2025-02-18T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/the-separation-of-22-cannabinoids-including-new-and-emerging-compounds\/"},"modified":"2025-12-10T17:04:01","modified_gmt":"2025-12-10T17:04:01","slug":"the-separation-of-22-cannabinoids-including-new-and-emerging-compounds","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/de\/blog-fr\/gnbl4827\/the-separation-of-22-cannabinoids-including-new-and-emerging-compounds","title":{"rendered":"The Separation of 22 Cannabinoids, Including New and Emerging Compounds"},"content":{"rendered":"\n

Many cannabinoids have emerged worldwide that labs are interested in integrating into their current potency methods. These include delta-9-THC isomers such as exo-THC, (6aR,9R)-delta-10-THC, and (6aR,9S)-delta-10-THC. Additional cannabinoids of interest include derivatives of delta-9-THC such as THCO-acetate, a synthetic psychoactive cannabinoid, THCP, the most potent cannabinoid in the cannabis plant to-date, and 9S-HHC\/9R-HHC, which are the hydrogenated forms of delta-9-THC. <\/p>\n\n\n\n

When analyzing cannabinoids by LC-UV\/VIS the user is often limited by the chromatographic space available when adding cannabinoids to their methods, since all analytes must be resolved when using this detector. Restek offers many solutions for cannabinoid analysis using Raptor columns.  Raptor, Restek\u2019s superficially porous particle, is the gold-standard for highly efficient separations of cannabinoids. It is important to also discuss Ultra, one of Restek\u2019s fully porous particles, for cannabinoid separations, and the differences compared to Raptor.<\/p>\n\n\n\n

Fully porous particles have more surface area for the phase to be bonded and thus have a higher carbon load when compared to superficially porous particles. For comparison, the carbon load on a fully porous Ultra C18 3 \u03bcm particle is 20% compared to the carbon load on a Raptor ARC-18 2.7 \u03bcm which is 7%. Ultra C18 can also provide a cost-benefit compared to Raptor ARC-18 as it is priced more economically, but other factors such as analytical runtime, pressure, solvent consumption, etc. must also be considered. Reducing solvent consumption and instrument run time can be some of the benefits of using a Raptor ARC-18, and an example of this can be seen here<\/a> for the analysis of 16 cannabinoids in 10 minutes.  <\/p>\n\n\n\n

Now that we understand the differences of Raptor and Ultra C18 columns, let\u2019s look at a very challenging cannabinoid separation. In this work, 22 commonly requested cannabinoids were analyzed by LC-VU\/VIS with a cycle time of 21 minutes. <\/p>\n\n\n

Figure 1:\u00a0<\/strong>22 Cannabinoids on Ultra C18 by LC-UV\/VIS<\/div>
\n
\"22<\/div>

LC_FF0631<\/p>

Peaks<\/h4>\n
<\/th>Peaks<\/th>tR<\/sub> (min)<\/th><\/tr><\/thead>\n
1.<\/td>Cannabidivarinic acid (CBDVA)<\/a><\/td>2.79<\/td><\/tr>\n
2.<\/td>Cannabidivarin (CBDV)<\/a><\/td>3.22<\/td><\/tr>\n
3.<\/td>Cannabidiolic acid (CBDA)<\/a><\/td>4.26<\/td><\/tr>\n
4.<\/td>Cannabigerolic acid (CBGA)<\/a><\/td>4.54<\/td><\/tr>\n
5.<\/td>Cannabigerol (CBG)<\/a><\/td>4.92<\/td><\/tr>\n
6.<\/td>Cannabidiol (CBD)<\/a><\/td>5.29<\/td><\/tr>\n
7.<\/td>Tetrahydrocannabivarin (THCV)<\/a><\/td>5.95<\/td><\/tr>\n
8.<\/td>Tetrahydrocannabivarinic acid (THCVA)<\/a><\/td>7.80<\/td><\/tr>\n
9.<\/td>Cannabinol (CBN)<\/a><\/td>8.64<\/td><\/tr>\n
10.<\/td>Cannabinolic acid (CBNA)<\/a><\/td>10.00<\/td><\/tr>\n
11.<\/td>Exo-tetrahydrocannabinol (exo-THC)<\/a><\/td>10.80<\/td><\/tr>\n<\/tbody><\/table>\n\n
<\/th>Peaks<\/th>tR<\/sub> (min)<\/th><\/tr><\/thead>
12.<\/td>d9-Tetrahydrocannabinol (9-THC)<\/a><\/td>11.17<\/td><\/tr>\n
13.<\/td>d8-Tetrahydrocannabinol (8-THC)<\/a><\/td>11.75<\/td><\/tr>\n
14.<\/td>(6aR,9S)-delta-10-Tetrahydrocannabinol (9S-10-THC)<\/a><\/td>13.26<\/td><\/tr>\n
15.<\/td>9(R)-Hexahydrocannabinol (9(R)-HHC)<\/a><\/td>13.66<\/td><\/tr>\n
16.<\/td>(6aR,9R)-delta-10-Tetrahydrocannabinol (9R-10-THC)<\/a><\/td>14.02<\/td><\/tr>\n
17.<\/td>9(S)-Hexahydrocannabinol (9(S)-HHC)<\/a><\/td>14.41<\/td><\/tr>\n
18.<\/td>Cannabichromene (CBC)<\/a><\/td>15.09<\/td><\/tr>\n
19.<\/td>9-Tetrahydrocannabinolic acid-A (THCA)<\/a><\/td>15.62<\/td><\/tr>\n
20.<\/td>Cannabichromenic acid (CBCA)<\/a><\/td>17.57<\/td><\/tr>\n
21.<\/td>9-Tetrahydrocannabiphorol (THCP)<\/a><\/td>18.85<\/td><\/tr>\n
22.<\/td>9-Tetrahydrocannabinol Acetate (THCO-Acetate)<\/td>19.34<\/td><\/tr>\n<\/tbody><\/table><\/div>

Conditions<\/h4>
Column<\/th>Ultra C18 (cat.# 9174365<\/a>)<\/td><\/tr>
Dimensions:<\/th>150 mm x 4.6 mm ID<\/td><\/tr>
Particle Size:<\/th>3 \u00b5m<\/td><\/tr>
Pore Size:<\/th>100 \u00c5<\/td><\/tr>
<\/td>
Guard Column:<\/th>UltraShield UHPLC precolumn filter, 0.2 \u00b5m frit (cat.# 25810<\/a>)<\/td><\/tr>
Temp.:<\/th>30 \u00b0C<\/td><\/tr>
Standard\/Sample<\/th><\/td><\/tr>
<\/td>Cannabinoids acids 7 standard (cat.# 34144<\/a>)<\/td><\/tr>
<\/td>Other standards were obtained separately.<\/td><\/tr>\n
Diluent:<\/th>25:75 Water:acetonitrile<\/td><\/tr>
Conc.:<\/th>50 ppm<\/td><\/tr><\/td><\/tr>
Inj. Vol.:<\/th>5 \u00b5L <\/td><\/tr>
Mobile Phase<\/th><\/td><\/tr>
A:<\/th>Water:acetonitrile (25:75), 2.5 mM ammonium formate, 0.1% formic acid <\/td><\/tr>
B:<\/th>Methanol, 0.1% formic acid <\/td><\/tr>
<\/td>
Time (min)<\/th>Flow (mL\/min)<\/th>%A<\/th>%B<\/th><\/tr><\/thead>
0.00<\/td>1.5<\/td>100<\/td>0<\/td><\/tr>
10.00<\/td>1.5<\/td>90<\/td>10<\/td><\/tr>
14.00<\/td>1.5<\/td>90<\/td>10<\/td><\/tr>
19.00<\/td>1.5<\/td>0<\/td>100<\/td><\/tr>
19.01<\/td>1.5<\/td>100<\/td>0<\/td><\/tr>
21.00<\/td>1.5<\/td>100<\/td>0<\/td><\/tr><\/tbody><\/table><\/td><\/tr>
Max Pressure:<\/th>269 bar<\/td><\/tr><\/table>
Detector<\/th>UV\/Vis @ 228 nm<\/td><\/tr>
Flow Cell Size:<\/th>500 nL <\/td><\/tr>
Instrument<\/th>Waters ACQUITY UPLC H-Class<\/td><\/tr>
Sample Preparation<\/th>Standards were aliquoted into 2 mL, screw-thread vials (cat.# 21143<\/a>) and capped with short-cap, screw-vial closures (cat.# 24498<\/a>).
<\/td><\/tr><\/table><\/div><\/div><\/div>
<\/path><\/g><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n
<\/div>\n\n\n\n

\u00a0By using this method, labs can achieve baseline separation for 22 cannabinoids. Is your lab interested in implementing this method? Contact your sales representative<\/a>.<\/p>\n\n\n\n

<\/div>\n\n\n
\n

Products Mentioned<\/h3>\n
\n
\n
\n
\n Catalog No. 9174365 <\/a> <\/div>\n
\n
Ultra C18, 3 \u00b5m, 150×4.6 mm HPLC-S\u00e4ule<\/div>\n <\/div>\n
\n Produkt anzeigen<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 25810 <\/a> <\/div>\n
\n
UltraShield UHPLC Vors\u00e4ulen-Filter, 0.2 \u03bcm Fritte, 5er Pack<\/div>\n <\/div>\n
\n Produkt anzeigen<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 34144 <\/a> <\/div>\n
\n
Cannabinoids\u00e4uren 7 Standard, 1000 \u00b5g\/mL, Acetonitril mit 1 % DIPEA und 0.05 % Ascorbins\u00e4ure, 1 mL\/Ampulle<\/div>\n <\/div>\n
\n Produkt anzeigen<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 21143 <\/a> <\/div>\n
\n
Short-Cap-Vial mit graduiertem Markierungsfeld, 9-425 Screw-Thread, 2.0 mL, 9 mm, 12 x 32 (nur Vial), braun, 1000er Pack<\/div>\n <\/div>\n
\n Produkt anzeigen<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 24498 <\/a> <\/div>\n
\n
Short Screw Kappen, Polypropylen, Screw-Thread, PTFE\/Silikon\/PTFE Septen, blau, vormontiert, 2.0 mL, 9 mm, 1000er Pack<\/div>\n <\/div>\n
\n Produkt anzeigen<\/a>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n \n\n\n
<\/div>\n","protected":false},"excerpt":{"rendered":"

Achieve baseline separation for 22 cannabinoids using this method!<\/p>\n","protected":false},"author":47,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[791],"tags":[],"industries-application":[2279,2198],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[2318],"hf_cat_post":[623],"ppma_author":[579],"class_list":["post-51821","post","type-post","status-publish","format-standard","hentry","category-blog-fr","industries-application-cannabis-fr","industries-application-cannabis-medical-recreatif","resource-technique-chromatographie-en-phase-liquide"],"acf":[],"taxonomy_info":{"category":[{"value":791,"label":"Blog"}],"industries-application":[{"value":2279,"label":"Cannabis"},{"value":2198,"label":"Cannabis m\u00e9dical ou r\u00e9cr\u00e9atif"}],"resource-technique":[{"value":2318,"label":"Chromatographie en phase liquide"}]},"featured_image_src_large":false,"author_info":{"display_name":"Jamie L. York, PhD","author_link":"https:\/\/discover.restek.com\/de\/author\/jamie-l-york-phd\/"},"comment_info":0,"category_info":[{"term_id":791,"name":"Blog","slug":"blog-fr","term_group":0,"term_taxonomy_id":791,"taxonomy":"category","description":"","parent":0,"count":442,"filter":"raw","cat_ID":791,"category_count":442,"category_description":"","cat_name":"Blog","category_nicename":"blog-fr","category_parent":0}],"tag_info":false,"authors":[{"term_id":579,"user_id":47,"is_guest":0,"slug":"jamie-l-york-phd","display_name":"Jamie L. York, PhD","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/york-jamie-1000x1000-1-e1765483005988.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/york-jamie-1000x1000-1-e1765483005988.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/51821","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/users\/47"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/comments?post=51821"}],"version-history":[{"count":6,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/51821\/revisions"}],"predecessor-version":[{"id":73036,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/51821\/revisions\/73036"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/media?parent=51821"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/categories?post=51821"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/tags?post=51821"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/industries-application?post=51821"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/post-badge?post=51821"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-type?post=51821"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/product-library?post=51821"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-technique?post=51821"},{"taxonomy":"hf_cat_post","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/hf_cat_post?post=51821"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/ppma_author?post=51821"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}