{"id":51843,"date":"2025-03-24T00:00:00","date_gmt":"2025-03-24T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/using-the-ezlc-modeler-for-cannabinoid-separations-part-3\/"},"modified":"2026-01-28T16:02:57","modified_gmt":"2026-01-28T16:02:57","slug":"using-the-ezlc-modeler-for-cannabinoid-separations-part-3","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/de\/blogs\/gnbl4850\/using-the-ezlc-modeler-for-cannabinoid-separations-part-3","title":{"rendered":"Using the <em>EZ<\/em>LC Modeler for Cannabinoid Separations\u2013Part 3: What You\u2019re Not Monitoring for Still Matters"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><style>     ul li { margin-top: 5px; }     ol li { margin-top: 5px; }  <\/style>If you haven\u2019t read the first two installments of this four-part blog series, they can be found here: <a href=\"https:\/\/discover.restek.com\/gnbl4848\/using-the-ezlc-modeler-for-cannabinoid-separations-part-1\/\">Optimization of an Existing Method<\/a>&nbsp;and <a href=\"https:\/\/discover.restek.com\/gnbl4849\/using-the-ezlc-modeler-for-cannabinoid-separations-part-2\/\">Using <em>EZ<\/em>LC Software to Monitor Effects of Ammonium Formate Concentrations on Cannabinoid Separations<\/a>.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">While target analytes in the method are the main focus, it is also important to know what could be in your sample that you are not monitoring for. A prime example of this is when a regulatory body rolled out a method in 2019. The disseminated regulations, which later received many revisions, were for cannabinoid testing of dried flower, including non-infused pre-rolls. The criterion for this testing is as follows:<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">1. Compounds: CBDA, CBG, CBD, THCV, CBN, \u03949-THC, \u03948-THC, CBC, THCA<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">2. HPLC Column: Restek Raptor ARC-18 2.1 x 150 mm, 2.7 \u00b5m (cat.#&nbsp;9314A62) or an equivalent column that can separate the cannabinoids of interest to achieve a minimum resolution of 1.3<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">3. Mobile Phases:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Mobile phase A: Water with 0.05 volume\/volume% formic acid<\/li>\n\n\n\n<li>Mobile phase B: Acetonitrile with 0.05 volume\/volume% formic acid<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">4. Flow Rate: 0.4 mL\/min<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">5. Run time: total 12.00 min (7.00 min + 2.00 min washing period + 3.00 min column re-equilibration)<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">6. Column Temperature: 35 \u2070C<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">7. Gradient:<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td><strong>Time (min)<\/strong><\/td><td><strong>Flow rate (mL\/min)<\/strong><\/td><td><strong>% Mobile Phase A<\/strong><\/td><td><strong>% Mobile Phase B<\/strong><\/td><\/tr><tr><td>0.00<\/td><td>0.4<\/td><td>25<\/td><td>75<\/td><\/tr><tr><td>7.00<\/td><td>0.4<\/td><td>25<\/td><td>75<\/td><\/tr><tr><td>7.01<\/td><td>0.4<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>9.00<\/td><td>0.4<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>9.01<\/td><td>0.4<\/td><td>25<\/td><td>75<\/td><\/tr><tr><td>12.00<\/td><td>0.4<\/td><td>25<\/td><td>75<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"wp-block-paragraph\">When labs began testing and validating this method, they determined there was a coelution caused by an analyte not in the method. Due to the regulations, labs are not permitted to change the method parameters but are required to state how\/why there could be an interference, along with how they would rectify it.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Below is an example, provided by Tania Sasaki, PhD, of Confidence Analytics. The unmonitored analyte, CBNA, is coeluting with the critical pair, \u03949-THC and \u03948-THC.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 1:<\/strong>\u00a0Coelution of \u03949-THC and CBNA<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/blog-part-3-using-EZLC-cannabinoid-separations-01.jpg\" alt=\"\" title=\"-\"><\/figure>\n\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 2:<\/strong>\u00a0Coelution of \u03948-THC and CBNA<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/blog-part-3-using-EZLC-cannabinoid-separations-02.jpg\" alt=\"\" title=\"-\"><\/figure>\n\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"wp-block-paragraph\">By using <em>EZ<\/em>LC modeling software, it is easy to determine if a method will have coelutions. By changing the concentration of ammonium formate and formic acid you can see all analytes are resolved with the desired USP resolution of 1.3 and above.<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/blog-part-3-using-EZLC-cannabinoid-separations-03.jpg\" alt=\"conditions interface in the ezlc modeler\" title=\"-\"><\/figure>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0705.png\" alt=\"Pro EZLC Comparison\u2014DCC Method\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_GN0705<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Experimental t<sub>R<\/sub><\/th><th style=\"text-align: center;width: 75px\">Modeled t<sub>R<\/sub><\/th><th style=\"text-align: center;width: 75px\">Difference (sec)<\/th><th style=\"text-align: center;width: 75px\">Run Time Difference (%)<\/th><th style=\"text-align: center;width: 75px\">USP Resolution<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabidivarinic acid (CBDVA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/31932-13-5\/Cannabidivarinic acid\" target=\"_blank\" rel=\"noopener\">Cannabidivarinic acid (CBDVA)<\/a><\/td><td class=\"oth\">1.47<\/td><td class=\"oth\">1.32<\/td><td class=\"oth\">9.12<\/td><td class=\"oth\">2.2<\/td><td class=\"oth\"><\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabidivarin (CBDV)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/24274-48-4\/Cannabidivarin\" target=\"_blank\" rel=\"noopener\">Cannabidivarin (CBDV)<\/a><\/td><td class=\"oth\">1.63<\/td><td class=\"oth\">1.46<\/td><td class=\"oth\">10.26<\/td><td class=\"oth\">2.4<\/td><td class=\"oth\">3.2<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabidiolic acid (CBDA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1244-58-2\/Cannabidiolic acid\" target=\"_blank\" rel=\"noopener\">Cannabidiolic acid (CBDA)<\/a><\/td><td class=\"oth\">2.03<\/td><td class=\"oth\">1.81<\/td><td class=\"oth\">13.26<\/td><td class=\"oth\">3.2<\/td><td class=\"oth\">7.1<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabigerolic acid (CBGA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/25555-57-1\/Cannabigerolic acid\" target=\"_blank\" rel=\"noopener\">Cannabigerolic acid (CBGA)<\/a><\/td><td class=\"oth\">2.14<\/td><td class=\"oth\">1.89<\/td><td class=\"oth\">15.24<\/td><td class=\"oth\">3.6<\/td><td class=\"oth\">1.7<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabigerol (CBG)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/25654-31-3\/Cannabigerol\" target=\"_blank\" rel=\"noopener\">Cannabigerol (CBG)<\/a><\/td><td class=\"oth\">2.27<\/td><td class=\"oth\">2.01<\/td><td class=\"oth\">15.78<\/td><td class=\"oth\">3.8<\/td><td class=\"oth\">1.9<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabidiol (CBD)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/13956-29-1\/Cannabidiol\" target=\"_blank\" rel=\"noopener\">Cannabidiol (CBD)<\/a><\/td><td class=\"oth\">2.40<\/td><td class=\"oth\">2.16<\/td><td class=\"oth\">14.64<\/td><td class=\"oth\">3.5<\/td><td class=\"oth\">1.9<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tetrahydrocannabivarin (THCV)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/31262-37-0\/Tetrahydrocannabivarin\" target=\"_blank\" rel=\"noopener\">Tetrahydrocannabivarin (THCV)<\/a><\/td><td class=\"oth\">2.59<\/td><td class=\"oth\">2.39<\/td><td class=\"oth\">11.82<\/td><td class=\"oth\">2.8<\/td><td class=\"oth\">2.6<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tetrahydrocannabivarinic acid (THCVA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/39986-26-0\/Tetrahydrocannabivarinic acid\" target=\"_blank\" rel=\"noopener\">Tetrahydrocannabivarinic acid (THCVA)<\/a><\/td><td class=\"oth\">3.25<\/td><td class=\"oth\">3.02<\/td><td class=\"oth\">13.92<\/td><td class=\"oth\">3.3<\/td><td class=\"oth\">8.1<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabinol (CBN)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/521-35-7\/Cannabinol\" target=\"_blank\" rel=\"noopener\">Cannabinol (CBN)<\/a><\/td><td class=\"oth\">3.50<\/td><td class=\"oth\">3.21<\/td><td class=\"oth\">17.28<\/td><td class=\"oth\">4.1<\/td><td class=\"oth\">2.6<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabinolic acid (CBNA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2808-39-1\/Cannabinolic acid\" target=\"_blank\" rel=\"noopener\">Cannabinolic acid (CBNA)<\/a><\/td><td class=\"oth\">4.20<\/td><td class=\"oth\">3.89<\/td><td class=\"oth\">18.42<\/td><td class=\"oth\">4.4<\/td><td class=\"oth\">6.6<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for \u03949-Tetrahydrocannabinol (\u03949-THC)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1972-08-3\/\u03949-Tetrahydrocannabinol\" target=\"_blank\" rel=\"noopener\">\u03949-Tetrahydrocannabinol (\u03949-THC)<\/a><\/td><td class=\"oth\">4.39<\/td><td class=\"oth\">4.11<\/td><td class=\"oth\">16.74<\/td><td class=\"oth\">4.0<\/td><td class=\"oth\">1.6<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for \u03948-Tetrahydrocannabinol (\u03948-THC)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/5957-75-5\/\u03948-Tetrahydrocannabinol\" target=\"_blank\" rel=\"noopener\">\u03948-Tetrahydrocannabinol (\u03948-THC)<\/a><\/td><td class=\"oth\">4.54<\/td><td class=\"oth\">4.27<\/td><td class=\"oth\">16.20<\/td><td class=\"oth\">3.9<\/td><td class=\"oth\">1.3<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabicyclol (CBL)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/21366-63-2\/Cannabicyclol\" target=\"_blank\" rel=\"noopener\">Cannabicyclol (CBL)<\/a><\/td><td class=\"oth\">5.21<\/td><td class=\"oth\">4.95<\/td><td class=\"oth\">15.30<\/td><td class=\"oth\">3.6<\/td><td class=\"oth\">5.2<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabichromene (CBC)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/20675-51-8\/Cannabichromene\" target=\"_blank\" rel=\"noopener\">Cannabichromene (CBC)<\/a><\/td><td class=\"oth\">5.47<\/td><td class=\"oth\">5.16<\/td><td class=\"oth\">18.78<\/td><td class=\"oth\">4.5<\/td><td class=\"oth\">1.9<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tetrahydrocannabinolic acid A (THCA-A)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/23978-85-0\/Tetrahydrocannabinolic acid A\" target=\"_blank\" rel=\"noopener\">Tetrahydrocannabinolic acid A (THCA-A)<\/a><\/td><td class=\"oth\">5.73<\/td><td class=\"oth\">5.43<\/td><td class=\"oth\">17.88<\/td><td class=\"oth\">4.3<\/td><td class=\"oth\">1.7<\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabichromenic acid (CBCA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/185505-15-1\/Cannabichromenic acid\" target=\"_blank\" rel=\"noopener\">Cannabichromenic acid (CBCA)<\/a><\/td><td class=\"oth\">6.45<\/td><td class=\"oth\">6.09<\/td><td class=\"oth\">21.54<\/td><td class=\"oth\">5.1<\/td><td class=\"oth\">4.4<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor ARC-18  (<a class=\"ga-cgram-prod-link\" target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9314A62\" rel=\"noopener\">cat.# 9314A62<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>150 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><td><\/td><td>Cannabinoids acids 7 standard, 1000 \u00b5g\/mL, acetonitrile with 1% DIPEA and 0.05% ascorbic acid (<a class=\"ga-cgram-prod-link\" target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/34144\" rel=\"noopener\">cat.# 34144<\/a>)<\/td><\/tr><tr><td><\/td><td>Cannabinoids neutrals 9 standard, 1000 \u00b5g\/mL, P&amp;T methanol, 1 mL\/ampul (<a class=\"ga-cgram-prod-link\" target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/34132\" rel=\"noopener\">cat.# 34132<\/a>)<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Acetonitrile<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>50 ppm<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 3 mM ammonium formate, 0.1 % formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile, 0.1 % formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>25<\/td><td>75<\/td><\/tr><tr><td>7.00<\/td><td>0.4<\/td><td>25<\/td><td>75<\/td><\/tr><tr><td>7.01<\/td><td>0.4<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>9.00<\/td><td>0.4<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>9.01<\/td><td>0.4<\/td><td>25<\/td><td>75<\/td><\/tr><tr><td>12.00<\/td><td>0.4<\/td><td>25<\/td><td>75<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>UV\/Vis @ 228 nm<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Flow Cell Size:<\/th><td>500 nL <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Waters ACQUITY UPLC H-Class<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>Working standard was prepared in a 2 mL, 9 mm amber vial (cat. 21142) by diluting 50 \u00b5L of each standard into 900 \u00b5L acetonitrile and capped with a 9 mm short screw cap (cat. 24497).<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-related-skus\"><h4>Related SKUs<\/h4><ul><li>34144<\/li><li>34132<\/li><li>9314A62<\/li><\/ul><\/div><div class=\"chromatogram-related-searches\"><h4>Related Searches<\/h4><ul><li>DCC<\/li><li>Department of Cannabis Control<\/li><li>EZLC<\/li><li>cannabinoid<\/li><li>separations<\/li><\/ul><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0705.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<p class=\"wp-block-paragraph\">&nbsp;In the coelution above, \u03949-THC is one of the analytes in question. Without adequate separation of \u03949-THC from other analytes in the method, hemp could be misidentified as cannabis. This coelution can also cause inflated THC numbers, leading to potential potency inflation whether intentional or unintentional.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">In summary, even if you are not required or do not want to monitor a larger panel of cannabinoids, it is important that during method development you consider all compounds that could be present to ensure accurate identification and quantitative results.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Tune in for the final installment of this series to learn how to harness the full capability of your column.<\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Resources and Further Reading<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">H.R.5485 &#8211; 115th Congress (2017-2018): Hemp Farming Act of 2018 | congress.gov | library of Congress. (n.d.-a). <a href=\"https:\/\/www.congress.gov\/bill\/115th-congress\/house-bill\/5485\" target=\"_blank\" rel=\"noopener\">https:\/\/www.congress.gov\/bill\/115th-congress\/house-bill\/5485<\/a><\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Check Out the Full Blog Series in the Related Resources below.<\/strong><\/p>\n\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">Erw\u00e4hnte Produkte<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/9314A62\">                                Art.-Nr. 9314A62                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Raptor ARC-18, 2.7 \u00b5m, 150&#215;2.1 mm HPLC-S\u00e4ule<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/9314A62\">Produkt anzeigen<\/a>\n                                                    <\/div>\n                    <\/div>\n                            <\/div>\n        <\/div>\n        \n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>The third in a four-part series, this blog explores how to ensure nontarget compounds aren&#8217;t interfering with your cannabinoids analysis using the free <em>EZ<\/em>LC chromatogram modeler.<\/p>\n","protected":false},"author":39,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[9],"tags":[],"industries-application":[2149,2151],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[2299],"ppma_author":[459],"class_list":["post-51843","post","type-post","status-publish","format-standard","hentry","category-blogs","industries-application-cannabis","industries-application-medical-or-recreational-cannabis","resource-technique-liquid-chromatography"],"acf":[],"taxonomy_info":{"category":[{"value":9,"label":"Blogs"}],"industries-application":[{"value":2149,"label":"Cannabis"},{"value":2151,"label":"Medical or Recreational Cannabis"}],"resource-technique":[{"value":2299,"label":"Liquid Chromatography"}]},"featured_image_src_large":false,"author_info":{"display_name":"Melinda Urich","author_link":"https:\/\/discover.restek.com\/de\/author\/melinda-urich\/"},"comment_info":0,"category_info":[{"term_id":9,"name":"Blogs","slug":"blogs","term_group":0,"term_taxonomy_id":9,"taxonomy":"category","description":"","parent":0,"count":437,"filter":"raw","cat_ID":9,"category_count":437,"category_description":"","cat_name":"Blogs","category_nicename":"blogs","category_parent":0}],"tag_info":false,"authors":[{"term_id":459,"user_id":39,"is_guest":0,"slug":"melinda-urich","display_name":"Melinda Urich","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-ulrich-melinda.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-ulrich-melinda.jpg"},"author_category":"1","first_name":"Melinda","last_name":"Urich","user_url":"https:\/\/www.linkedin.com\/in\/melinda-urich-997b81225\/","job_title":"","description":"Melinda \u201cMel\u201d Urich is an applications scientist in the LC Solutions department. Her primary focus is on the development of novel applications in the cannabis and food markets. In her previous role at Restek as an LC manufacturing chemist, she led the synthesis of silica, bonding of stationary phases as well as new process implementations and improvements. Mel attended Juniata College where she earned her BS in Chemistry and performed research in Atomic Force Microscopy AFM)."}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/51843","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/users\/39"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/comments?post=51843"}],"version-history":[{"count":9,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/51843\/revisions"}],"predecessor-version":[{"id":85521,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/51843\/revisions\/85521"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/media?parent=51843"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/categories?post=51843"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/tags?post=51843"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/industries-application?post=51843"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/post-badge?post=51843"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-type?post=51843"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/product-library?post=51843"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-technique?post=51843"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/ppma_author?post=51843"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}