{"id":60807,"date":"2025-11-07T15:20:39","date_gmt":"2025-11-07T15:20:39","guid":{"rendered":"https:\/\/discover.restek.com\/?p=60807"},"modified":"2026-01-08T14:22:18","modified_gmt":"2026-01-08T14:22:18","slug":"the-detection-of-flavonoids-in-hemp-flower-by-lc-ms-ms","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/de\/posters-presentations-ja\/gnot5327\/the-detection-of-flavonoids-in-hemp-flower-by-lc-ms-ms","title":{"rendered":"The Detection of Flavonoids in Hemp Flower by LC-MS\/MS"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">Flavonoids are a class of compounds that are endogenous in hemp and cannabis that are antioxidant rich and can affect the flavor profile. Routine testing in cannabis labs oftentimes does not include flavonoid testing, but as the market for cannabis grows, industry leaders may choose to implement new tests to aid the advancement of the science of cannabis and to differentiate their services from their competitors. In this work, a method to detect 19 flavonoids in CBD- and CBG-dominant hemp flower was developed using LC-MS\/MS. Extraction of flavonoids was performed by weighing 0.5 g of ground hemp flower and adding 80\/20 methanol\/water (10 mL) to the hemp flower and vortexing. The samples were sonicated for 15 min, centrifuged at 4200 rpm for 5 min, and diluted 50-fold. The analytical method was developed using LC-MS\/MS with electrospray ionization in positive and negative ion modes. The method uses gradient conditions on a Raptor Biphenyl 100 x 2.1, 2.7 \u00b5m column equipped with a guard cartridge. Ten flavonoids were detected in the CBG hemp flower sample, and 12 flavonoids were detected in the CBD hemp flower samples. Single point recovery experiments were also performed at 100 ppb using two deuterated internal standards. The results of the single point recovery ranged from 80 \u2013 104%. The developed method was able to resolve isobars within a cycle time of 7 minutes, and acceptable recoveries were achieved for both hemp flower samples, indicating this is an effective procedure for the extraction of flavonoids from hemp flower.<\/p>\n\n\n\n<div data-wp-interactive=\"core\/file\" class=\"wp-block-file\"><object data-wp-bind--hidden=\"!state.hasPdfPreview\" hidden class=\"wp-block-file__embed\" data=\"https:\/\/discover.restek.com\/wp-content\/uploads\/poster-aoac-2022-detection-flavonoids-hemp-flower.pdf\" type=\"application\/pdf\" style=\"width:100%;height:600px\" aria-label=\"Embed of poster-aoac-2022-detection-flavonoids-hemp-flower.\"><\/object><a id=\"wp-block-file--media-95b1528b-7695-4eea-a5ea-1973d5b2843d\" href=\"https:\/\/discover.restek.com\/wp-content\/uploads\/poster-aoac-2022-detection-flavonoids-hemp-flower.pdf\">poster-aoac-2022-detection-flavonoids-hemp-flower<\/a><a href=\"https:\/\/discover.restek.com\/wp-content\/uploads\/poster-aoac-2022-detection-flavonoids-hemp-flower.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-95b1528b-7695-4eea-a5ea-1973d5b2843d\">Download<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Flavonoids are a class of compounds that are endogenous in hemp and cannabis that are antioxidant rich and can affect the flavor profile. Routine testing in cannabis labs oftentimes does not include flavonoid testing, but as the market for cannabis grows, industry leaders may choose to implement new tests to aid the advancement of the science of cannabis and to differentiate their services from their competitors. In this work, a method to detect 19 flavonoids in CBD- and CBG-dominant hemp flower was developed using LC-MS\/MS. Extraction of flavonoids was performed by weighing 0.5 g of ground hemp flower and adding 80\/20 methanol\/water (10 mL) to the hemp flower and vortexing. The samples were sonicated for 15 min, centrifuged at 4200 rpm for 5 min, and diluted 50-fold. The analytical method was developed using LC-MS\/MS with electrospray ionization in positive and negative ion modes. The method uses gradient conditions on a Raptor Biphenyl 100 x 2.1, 2.7 \u00b5m column equipped with a guard cartridge. Ten flavonoids were detected in the CBG hemp flower sample, and 12 flavonoids were detected in the CBD hemp flower samples. Single point recovery experiments were also performed at 100 ppb using two deuterated internal standards. The results of the single point recovery ranged from 80 \u2013 104%. The developed method was able to resolve isobars within a cycle time of 7 minutes, and acceptable recoveries were achieved for both hemp flower samples, indicating this is an effective procedure for the extraction of flavonoids from hemp flower.<\/p>\n","protected":false},"author":47,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[810],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[],"ppma_author":[648,579],"class_list":["post-60807","post","type-post","status-publish","format-standard","hentry","category-posters-presentations-ja"],"acf":[],"taxonomy_info":{"category":[{"value":810,"label":"Posters &amp; Presentations"}]},"featured_image_src_large":false,"author_info":{"display_name":"Jamie L. York, PhD","author_link":"https:\/\/discover.restek.com\/de\/author\/jamie-l-york-phd\/"},"comment_info":0,"category_info":[{"term_id":810,"name":"Posters &amp; Presentations","slug":"posters-presentations-ja","term_group":0,"term_taxonomy_id":810,"taxonomy":"category","description":"","parent":0,"count":118,"filter":"raw","cat_ID":810,"category_count":118,"category_description":"","cat_name":"Posters &amp; Presentations","category_nicename":"posters-presentations-ja","category_parent":0}],"tag_info":false,"authors":[{"term_id":648,"user_id":0,"is_guest":1,"slug":"dan-delurio","display_name":"Dan DeLurio","avatar_url":"https:\/\/secure.gravatar.com\/avatar\/?s=96&d=mm&r=g","author_category":"1","first_name":"Dan","last_name":"DeLurio","user_url":"","job_title":"","description":""},{"term_id":579,"user_id":47,"is_guest":0,"slug":"jamie-l-york-phd","display_name":"Jamie L. York, PhD","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/york-jamie-1000x1000-1-e1765483005988.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/york-jamie-1000x1000-1-e1765483005988.jpg"},"author_category":"1","first_name":"Jamie","last_name":"York","user_url":"","job_title":"","description":"Jamie York is a principal scientist in the Applications Lab at Restek Corporation. She leads the development of innovative analytical methods tailored to the food, clinical, environmental, and cannabis markets. Jamie earned her PhD in chemistry from The University of Texas at Arlington, where she gained extensive expertise in a range of analytical techniques, including gas chromatography\u2013vacuum ultraviolet (GC\u2013VUV); gas chromatography\u2013mass spectrometry (GC\u2013MS); matrix-assisted laser desorption\/ionization (MALDI); and liquid chromatography\u2013 mass spectrometry (LC\u2013MS\/MS); with a research emphasis on food and environmental analysis. Today, her work focuses on complex method development and advanced sample preparation strategies to support the evolving needs of the scientific community."}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/60807","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/users\/47"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/comments?post=60807"}],"version-history":[{"count":1,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/60807\/revisions"}],"predecessor-version":[{"id":60809,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/60807\/revisions\/60809"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/media?parent=60807"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/categories?post=60807"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/tags?post=60807"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/industries-application?post=60807"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/post-badge?post=60807"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-type?post=60807"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/product-library?post=60807"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-technique?post=60807"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/ppma_author?post=60807"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}