{"id":76477,"date":"2014-08-12T00:00:00","date_gmt":"2014-08-12T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/?p=76477"},"modified":"2026-01-21T17:19:02","modified_gmt":"2026-01-21T17:19:02","slug":"which-lc-column-should-i-use-for-method-8330b-explosives-analysis","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/de\/blogs\/gnbl5554\/which-lc-column-should-i-use-for-method-8330b-explosives-analysis","title":{"rendered":"Which LC column should I use for Method 8330B explosives analysis?"},"content":{"rendered":"\n<p>Over the years, Restek has run applications on a long list of columns for this analysis. It does require a primary and secondary column for analysis, since there is not one column that perfectly separates all compounds on the list simultaneously. Keep in mind that&nbsp;<a href=\"http:\/\/www.epa.gov\/osw\/hazard\/testmethods\/pdfs\/8330b.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">EPA Method 8330B&nbsp;<\/a>allows for alternate columns versus the ones listed if you can demonstrate proficiency by presenting valid data that meets QC acceptance criteria as described in&nbsp;<a href=\"http:\/\/www.epa.gov\/osw\/hazard\/testmethods\/sw846\/pdfs\/8000b.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Method 8000<\/a>. Our Innovations chemists have worked hard to make column selection easier for this and get you started in the right direction for optimization.<\/p>\n\n\n\n<p>So far, we have determined optimal conditions for two pairs of columns that we recommend above all other combinations. For traditional HPLC systems with a 400 Bar pressure limit, we suggest using an&nbsp;Ultra C8&nbsp;as the primary column and an&nbsp;Ultra Aromax&nbsp;as the secondary (confirmatory) column.<\/p>\n\n\n\n<p>Here are chromatograms.&nbsp;<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><h3 class=\"article-title\">Explosives by EPA 8330B on Ultra C8 with Trident Direct Guard Column<\/h3><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0526.png\" alt=\"Explosives by EPA 8330B on Ultra C8 with Trident Direct Guard Column\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EV0526<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for HMX\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2691-41-0\/HMX\" target=\"_blank\" rel=\"noopener\">HMX<\/a><\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for RDX\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/121-82-4\/RDX\" target=\"_blank\" rel=\"noopener\">RDX<\/a><\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 1,3,5-Trinitrobenzene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/99-35-4\/1,3,5-Trinitrobenzene\" target=\"_blank\" rel=\"noopener\">1,3,5-Trinitrobenzene<\/a><\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 1,4-Dinitrobenzene (SS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/100-25-4\/1,4-Dinitrobenzene\" target=\"_blank\" rel=\"noopener\">1,4-Dinitrobenzene (SS)<\/a><\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 1,3-Dinitrobenzene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/99-65-0\/1,3-Dinitrobenzene\" target=\"_blank\" rel=\"noopener\">1,3-Dinitrobenzene<\/a><\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nitrobenzene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/98-95-3\/Nitrobenzene\" target=\"_blank\" rel=\"noopener\">Nitrobenzene<\/a><\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 3,5-Dinitroaniline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/618-87-1\/3,5-Dinitroaniline\" target=\"_blank\" rel=\"noopener\">3,5-Dinitroaniline<\/a><\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tetryl\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/479-45-8\/Tetryl\" target=\"_blank\" rel=\"noopener\">Tetryl<\/a><\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2,4,6-Trinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/118-96-7\/2,4,6-Trinitrotoluene\" target=\"_blank\" rel=\"noopener\">2,4,6-Trinitrotoluene<\/a><\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nitroglycerin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/55-63-0\/Nitroglycerin\" target=\"_blank\" rel=\"noopener\">Nitroglycerin<\/a><\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2,4-Dinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/121-14-2\/2,4-Dinitrotoluene\" target=\"_blank\" rel=\"noopener\">2,4-Dinitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 4-Amino-2,6-dinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/19406-51-0\/4-Amino-2,6-dinitrotoluene\" target=\"_blank\" rel=\"noopener\">4-Amino-2,6-dinitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2,6-Dinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/606-20-2\/2,6-Dinitrotoluene\" target=\"_blank\" rel=\"noopener\">2,6-Dinitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2-Amino-4,6-dinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/35572-78-2\/2-Amino-4,6-dinitrotoluene\" target=\"_blank\" rel=\"noopener\">2-Amino-4,6-dinitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2-Nitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/88-72-2\/2-Nitrotoluene\" target=\"_blank\" rel=\"noopener\">2-Nitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 4-Nitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/99-99-0\/4-Nitrotoluene\" target=\"_blank\" rel=\"noopener\">4-Nitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 3-Nitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/99-08-1\/3-Nitrotoluene\" target=\"_blank\" rel=\"noopener\">3-Nitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">18.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for PETN\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/78-11-5\/PETN\" target=\"_blank\" rel=\"noopener\">PETN<\/a><\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Ultra C8  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9103565?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0526\" rel=\"noopener\">cat.# 9103565<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>150 mm x 4.6 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>5 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>100 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><td><\/td><td>8330B Nitroaromatics and nitramine mix (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/33204?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0526\" rel=\"noopener\">cat.# 33204<\/a>)<\/td><\/tr><tr><td><\/td><td>1,4-Dinitrobenzene (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/33205?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0526\" rel=\"noopener\">cat.# 33205<\/a>)<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Deionized water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>20 \u00b5g\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td>Water:methanol (52:48)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Flow:<\/th><td>1.2 mL\/min<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>UV\/Vis @ 210\/254 nm<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Shimadzu UFLC<span class=\"smallcaps\">XR<\/span><\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Trident guard cartridge holder cat.# 25083 was used to produce this chromatogram, but has since been discontinued. For assistance choosing a replacement for this application, contact Restek Technical Service or your local Restek representative.<br \/>&#8211; &#8211; &#8211; &#8211; &#8211; &#8211;<br \/>Trident direct 10 mm in-line guard cartridge holder cat.# 25083<br \/>Ultra C8 10 mm x 4 mm guard cartridge (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/910350210?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0526\" rel=\"noopener\">cat.# 910350210<\/a>)<br \/><br \/>This is the recommended primary column for a 2 column analysis. See LC_EV0527 for the confirmation column.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0526.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><h3 class=\"article-title\">Explosives by EPA 8330B on Ultra Aromax with Trident Direct Guard<\/h3><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0527.png\" alt=\"Explosives by EPA 8330B on Ultra Aromax with Trident Direct Guard\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EV0527<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for HMX\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2691-41-0\/HMX\" target=\"_blank\" rel=\"noopener\">HMX<\/a><\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for RDX\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/121-82-4\/RDX\" target=\"_blank\" rel=\"noopener\">RDX<\/a><\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 1,3,5-Trinitrobenzene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/99-35-4\/1,3,5-Trinitrobenzene\" target=\"_blank\" rel=\"noopener\">1,3,5-Trinitrobenzene<\/a><\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 1,4-Dinitrobenzene (SS)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/100-25-4\/1,4-Dinitrobenzene\" target=\"_blank\" rel=\"noopener\">1,4-Dinitrobenzene (SS)<\/a><\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 1,3-Dinitrobenzene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/99-65-0\/1,3-Dinitrobenzene\" target=\"_blank\" rel=\"noopener\">1,3-Dinitrobenzene<\/a><\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nitrobenzene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/98-95-3\/Nitrobenzene\" target=\"_blank\" rel=\"noopener\">Nitrobenzene<\/a><\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 3,5-Dinitroaniline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/618-87-1\/3,5-Dinitroaniline\" target=\"_blank\" rel=\"noopener\">3,5-Dinitroaniline<\/a><\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tetryl\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/479-45-8\/Tetryl\" target=\"_blank\" rel=\"noopener\">Tetryl<\/a><\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2,4,6-Trinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/118-96-7\/2,4,6-Trinitrotoluene\" target=\"_blank\" rel=\"noopener\">2,4,6-Trinitrotoluene<\/a><\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nitroglycerin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/55-63-0\/Nitroglycerin\" target=\"_blank\" rel=\"noopener\">Nitroglycerin<\/a><\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2,4-Dinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/121-14-2\/2,4-Dinitrotoluene\" target=\"_blank\" rel=\"noopener\">2,4-Dinitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 4-Amino-2,6-dinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/19406-51-0\/4-Amino-2,6-dinitrotoluene\" target=\"_blank\" rel=\"noopener\">4-Amino-2,6-dinitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2,6-Dinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/606-20-2\/2,6-Dinitrotoluene\" target=\"_blank\" rel=\"noopener\">2,6-Dinitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2-Amino-4,6-dinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/35572-78-2\/2-Amino-4,6-dinitrotoluene\" target=\"_blank\" rel=\"noopener\">2-Amino-4,6-dinitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2-Nitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/88-72-2\/2-Nitrotoluene\" target=\"_blank\" rel=\"noopener\">2-Nitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 4-Nitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/99-99-0\/4-Nitrotoluene\" target=\"_blank\" rel=\"noopener\">4-Nitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 3-Nitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/99-08-1\/3-Nitrotoluene\" target=\"_blank\" rel=\"noopener\">3-Nitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">18.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for PETN\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/78-11-5\/PETN\" target=\"_blank\" rel=\"noopener\">PETN<\/a><\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Ultra Aromax  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9127565?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0527\" rel=\"noopener\">cat.# 9127565<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>150 mm x 4.6 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>5 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>100 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><td><\/td><td>8330B Nitroaromatics and nitramine mix (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/33204?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0527\" rel=\"noopener\">cat.# 33204<\/a>)<\/td><\/tr><tr><td><\/td><td>1,4-Dinitrobenzene (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/33205?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0527\" rel=\"noopener\">cat.# 33205<\/a>)<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Deionized water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>20 \u00b5g\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td>Water:methanol (30:70)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Flow:<\/th><td>1.2 mL\/min<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>UV\/Vis @ 210\/254 nm<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Shimadzu UFLC<span class=\"smallcaps\">XR<\/span><\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Trident guard cartridge holder cat.# 25083 was used to produce this chromatogram, but has since been discontinued. For assistance choosing a replacement for this application, contact Restek Technical Service or your local Restek representative.<br \/>&#8211; &#8211; &#8211; &#8211; &#8211; &#8211;<br \/>Trident direct 10 mm in-line guard cartridge holder cat.# 25083<br \/>Ultra Aromax 10 mm x 4 mm guard cartridge (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/912750210?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0527\" rel=\"noopener\">cat.# 912750210<\/a>)<br \/><br \/>This is the recommended confirmation column for a 2 column analysis. See LC_EV0526 for the primary column.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-article-inner\">\n\n<p><\/p>\n\n<\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0527.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>If you have a system that has a pressure limit of at least 600 Bar, we suggest our&nbsp;Raptor Biphenyl&nbsp;as the primary column and&nbsp;Raptor ARC-18&nbsp;as the secondary column. Here are chromatograms.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><h3 class=\"article-title\">Explosives on Raptor Biphenyl by EPA Method 8330B<\/h3><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0531.png\" alt=\"Explosives on Raptor Biphenyl by EPA Method 8330B\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EV0531<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for HMX\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2691-41-0\/HMX\" target=\"_blank\" rel=\"noopener\">HMX<\/a><\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for RDX\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/121-82-4\/RDX\" target=\"_blank\" rel=\"noopener\">RDX<\/a><\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 1,3,5-Trinitrobenzene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/99-35-4\/1,3,5-Trinitrobenzene\" target=\"_blank\" rel=\"noopener\">1,3,5-Trinitrobenzene<\/a><\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 1,4-Dinitrobenzene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/100-25-4\/1,4-Dinitrobenzene\" target=\"_blank\" rel=\"noopener\">1,4-Dinitrobenzene<\/a><\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 1,3-Dinitrobenzene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/99-65-0\/1,3-Dinitrobenzene\" target=\"_blank\" rel=\"noopener\">1,3-Dinitrobenzene<\/a><\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 3,5-Dinitroaniline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/618-87-1\/3,5-Dinitroaniline\" target=\"_blank\" rel=\"noopener\">3,5-Dinitroaniline<\/a><\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nitrobenzene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/98-95-3\/Nitrobenzene\" target=\"_blank\" rel=\"noopener\">Nitrobenzene<\/a><\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nitroglycerin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/55-63-0\/Nitroglycerin\" target=\"_blank\" rel=\"noopener\">Nitroglycerin<\/a><\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tetryl\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/479-45-8\/Tetryl\" target=\"_blank\" rel=\"noopener\">Tetryl<\/a><\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2,4,6-Trinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/118-96-7\/2,4,6-Trinitrotoluene\" target=\"_blank\" rel=\"noopener\">2,4,6-Trinitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2-Amino-4,6-dinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/35572-78-2\/2-Amino-4,6-dinitrotoluene\" target=\"_blank\" rel=\"noopener\">2-Amino-4,6-dinitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 4-Amino-2,6-dinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/19406-51-0\/4-Amino-2,6-dinitrotoluene\" target=\"_blank\" rel=\"noopener\">4-Amino-2,6-dinitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2,4-Dinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/121-14-2\/2,4-Dinitrotoluene\" target=\"_blank\" rel=\"noopener\">2,4-Dinitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2,6-Dinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/606-20-2\/2,6-Dinitrotoluene\" target=\"_blank\" rel=\"noopener\">2,6-Dinitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2-Nitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/88-72-2\/2-Nitrotoluene\" target=\"_blank\" rel=\"noopener\">2-Nitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 4-Nitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/99-99-0\/4-Nitrotoluene\" target=\"_blank\" rel=\"noopener\">4-Nitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 3-Nitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/99-08-1\/3-Nitrotoluene\" target=\"_blank\" rel=\"noopener\">3-Nitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">18.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for PETN\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/78-11-5\/PETN\" target=\"_blank\" rel=\"noopener\">PETN<\/a><\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Biphenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309A65?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0531\" rel=\"noopener\">cat.# 9309A65<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>150 mm x 4.6 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><td><\/td><td>8330B Nitroaromatics and nitramine mix (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/33204?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0531\" rel=\"noopener\">cat.# 33204<\/a>)<\/td><\/tr><tr><td><\/td><td>1,4-Dinitrobenzene (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/33205?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0531\" rel=\"noopener\">cat.# 33205<\/a>)<\/td><\/tr><tr><td><\/td><\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>20 \u00b5g\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>1.0<\/td><td>30<\/td><td>70<\/td><\/tr><tr><td>10<\/td><td>1.0<\/td><td>30<\/td><td>70<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>UV\/Vis @ 210\/254 nm<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Cell Temp:<\/th><td>35 \u00b0C<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Flow Cell Size:<\/th><td>Semi-micro <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Shimadzu Prominence<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>This is the recommended primary column for a two-column analysis. See LC_EV0530 for the confirmation column.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0531.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><h3 class=\"article-title\">Explosives on Raptor ARC-18 by EPA Method 8330B<\/h3><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0530.png\" alt=\"Explosives on Raptor ARC-18 by EPA Method 8330B\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EV0530<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for HMX\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2691-41-0\/HMX\" target=\"_blank\" rel=\"noopener\">HMX<\/a><\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for RDX\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/121-82-4\/RDX\" target=\"_blank\" rel=\"noopener\">RDX<\/a><\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 1,3,5-Trinitrobenzene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/99-35-4\/1,3,5-Trinitrobenzene\" target=\"_blank\" rel=\"noopener\">1,3,5-Trinitrobenzene<\/a><\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 1,4-Dinitrobenzene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/100-25-4\/1,4-Dinitrobenzene\" target=\"_blank\" rel=\"noopener\">1,4-Dinitrobenzene<\/a><\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 1,3-Dinitrobenzene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/99-65-0\/1,3-Dinitrobenzene\" target=\"_blank\" rel=\"noopener\">1,3-Dinitrobenzene<\/a><\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 3,5-Dinitroaniline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/618-87-1\/3,5-Dinitroaniline\" target=\"_blank\" rel=\"noopener\">3,5-Dinitroaniline<\/a><\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nitrobenzene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/98-95-3\/Nitrobenzene\" target=\"_blank\" rel=\"noopener\">Nitrobenzene<\/a><\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nitroglycerin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/55-63-0\/Nitroglycerin\" target=\"_blank\" rel=\"noopener\">Nitroglycerin<\/a><\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tetryl\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/479-45-8\/Tetryl\" target=\"_blank\" rel=\"noopener\">Tetryl<\/a><\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2,4,6-Trinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/118-96-7\/2,4,6-Trinitrotoluene\" target=\"_blank\" rel=\"noopener\">2,4,6-Trinitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2-Amino-4,6-dinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/35572-78-2\/2-Amino-4,6-dinitrotoluene\" target=\"_blank\" rel=\"noopener\">2-Amino-4,6-dinitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 4-Amino-2,6-dinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/19406-51-0\/4-Amino-2,6-dinitrotoluene\" target=\"_blank\" rel=\"noopener\">4-Amino-2,6-dinitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2,4-Dinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/121-14-2\/2,4-Dinitrotoluene\" target=\"_blank\" rel=\"noopener\">2,4-Dinitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2,6-Dinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/606-20-2\/2,6-Dinitrotoluene\" target=\"_blank\" rel=\"noopener\">2,6-Dinitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2-Nitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/88-72-2\/2-Nitrotoluene\" target=\"_blank\" rel=\"noopener\">2-Nitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 4-Nitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/99-99-0\/4-Nitrotoluene\" target=\"_blank\" rel=\"noopener\">4-Nitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 3-Nitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/99-08-1\/3-Nitrotoluene\" target=\"_blank\" rel=\"noopener\">3-Nitrotoluene<\/a><\/td><\/tr>\n<tr><td class=\"num\">18.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for PETN\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/78-11-5\/PETN\" target=\"_blank\" rel=\"noopener\">PETN<\/a><\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor ARC-18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9314A1E?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0530\" rel=\"noopener\">cat.# 9314A1E<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 3.0 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><td><\/td><td>8330B Nitroaromatics and nitramine mix (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/33204?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0530\" rel=\"noopener\">cat.# 33204<\/a>)<\/td><\/tr><tr><td><\/td><td>1,4-Dinitrobenzene (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/33205?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0530\" rel=\"noopener\">cat.# 33205<\/a>)<\/td><\/tr><tr><td><\/td><\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>20 \u00b5g\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>3 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>1.0<\/td><td>75<\/td><td>25<\/td><\/tr><tr><td>22.5<\/td><td>1.0<\/td><td>75<\/td><td>25<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>UV\/Vis @ 210\/254 nm<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Cell Temp:<\/th><td>35 \u00b0C<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Flow Cell Size:<\/th><td>Semi-micro <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Shimadzu UFLC<span class=\"smallcaps\">XR<\/span><\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>This is the recommended confirmation column for a two-column analysis. See LC_EV0531 for the primary column.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0530.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>I hope this clarifies some things and gets you started in the right direction. Thank you for reading.<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">Products Mentioned<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/9103565\">                                Catalog No. 9103565                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Ultra C8, 5 \u00b5m, 150&#215;4.6 mm HPLC-S\u00e4ule<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                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class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/910350210\">Produkt anzeigen<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/9127565\">                                Catalog No. 9127565                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Ultra Aromax, 5 \u00b5m, 150&#215;4.6 mm HPLC-S\u00e4ule<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/9127565\">Produkt anzeigen<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/912750210\">                                Catalog No. 912750210                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Ultra Aromax Vors\u00e4ulenkartusche, 5 \u00b5m, 10 x 4.0 mm, 3er Pack<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/912750210\">Produkt anzeigen<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/9314A1E\">                                Catalog No. 9314A1E                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Raptor ARC-18, 2.7 \u00b5m, 100&#215;3.0 mm HPLC-S\u00e4ule<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/9314A1E\">Produkt anzeigen<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/9309A65\">                                Catalog No. 9309A65                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Raptor Biphenyl, 2.7 \u00b5m, 150&#215;4.6 mm HPLC-S\u00e4ule<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/9309A65\">Produkt anzeigen<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/33204\">                                Catalog No. 33204                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Gemisch f\u00fcr Nitroaromaten und Nitramin-Sprengstoffe mittels HPLC, EPA 8330B, 1000 \u03bcg\/mL, Acetonitril, 1 mL\/Ampulle<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/33204\">Produkt anzeigen<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/33205\">                                Catalog No. 33205                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">8330 Interner Standard (1,4-Dinitrobenzol), 2000 \u03bcg\/mL, Acetonitril, 1 mL\/Ampulle<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/de.restek.com\/p\/33205\">Produkt anzeigen<\/a>\n                                                    <\/div>\n                    <\/div>\n                            <\/div>\n        <\/div>\n        \n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Over the years, Restek has run applications on a long list of columns for this analysis. It does require a primary and secondary column for analysis, since there is not one column that perfectly separates all compounds on the list&#8230;<\/p>\n","protected":false},"author":5,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[9],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[],"ppma_author":[602],"class_list":["post-76477","post","type-post","status-publish","format-standard","hentry","category-blogs"],"acf":[],"taxonomy_info":{"category":[{"value":9,"label":"Blogs"}]},"featured_image_src_large":false,"author_info":{"display_name":"Brenda Cogan","author_link":"https:\/\/discover.restek.com\/de\/author\/brenda-coganrestek-com\/"},"comment_info":0,"category_info":[{"term_id":9,"name":"Blogs","slug":"blogs","term_group":0,"term_taxonomy_id":9,"taxonomy":"category","description":"","parent":0,"count":438,"filter":"raw","cat_ID":9,"category_count":438,"category_description":"","cat_name":"Blogs","category_nicename":"blogs","category_parent":0}],"tag_info":false,"authors":[{"term_id":602,"user_id":0,"is_guest":1,"slug":"nancy-schwartz","display_name":"Nancy Schwartz","avatar_url":"https:\/\/secure.gravatar.com\/avatar\/?s=96&d=mm&r=g","0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/76477","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/comments?post=76477"}],"version-history":[{"count":3,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/76477\/revisions"}],"predecessor-version":[{"id":83655,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/posts\/76477\/revisions\/83655"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/media?parent=76477"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/categories?post=76477"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/tags?post=76477"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/industries-application?post=76477"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/post-badge?post=76477"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-type?post=76477"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/product-library?post=76477"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/resource-technique?post=76477"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/de\/wp-json\/wp\/v2\/ppma_author?post=76477"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}