{"id":2940,"date":"2025-07-30T18:30:12","date_gmt":"2025-07-30T18:30:12","guid":{"rendered":"https:\/\/discover.restek.com\/?p=2940"},"modified":"2025-12-09T00:05:33","modified_gmt":"2025-12-09T00:05:33","slug":"is-conditioning-necessary-for-spe-cartridges","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/es\/faq\/gnot5144\/is-conditioning-necessary-for-spe-cartridges","title":{"rendered":"Is conditioning necessary for SPE cartridges?"},"content":{"rendered":"\n<p>Yes. Detailed instructions for conditioning SPE cartridges can be found\u00a0below. All SPE cartridges should be conditioned to wet and settle the bed, activate the packing materials, and remove any residual process materials (e.g., fines).<\/p>\n\n\n\n<p>As a general guide:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Use one to two column volumes of the conditioning solution(s) recommended in the table below.<\/li>\n\n\n\n<li>Use a high flow rate.<\/li>\n\n\n\n<li>Do not allow the packing bed to go dry. The packing bed should be wet before adding sample.<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">Characteristics of Reversed- and Normal Phase SPE<\/h4>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><strong>Packing<\/strong><\/td><td><strong>Polarity<\/strong><\/td><td><strong>Sample Matrix<\/strong><\/td><td><strong>Conditioning Solution(s)<\/strong><\/td><td><strong>Elution Solution<\/strong><\/td><\/tr><tr><td>C18, carbon<\/td><td>packing and analytes are nonpolar<\/td><td>polar, often aqueous<\/td><td>(9a) methanol, then water or buffer (same as sample), or<br>(9b) final extraction solvent, then water-miscible solvent, then water or buffer<\/td><td>nonpolar solvent or mixed solvent solution<\/td><\/tr><tr><td>silica, Florisil, carbon<\/td><td>packing and analytes are polar<\/td><td>nonpolar, often an organic solvent<\/td><td>fresh solvent (same as sample)<\/td><td>fresh solvent<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><\/p>\n\n\n\n<div data-wp-interactive=\"core\/file\" class=\"wp-block-file\"><object data-wp-bind--hidden=\"!state.hasPdfPreview\" hidden class=\"wp-block-file__embed\" data=\"https:\/\/discover.restek.com\/wp-content\/uploads\/800-00-001.pdf\" type=\"application\/pdf\" style=\"width:100%;height:600px\" aria-label=\"Embed of 800-00-001.\"><\/object><a id=\"wp-block-file--media-44b5fb08-b036-4217-b308-aed780a03029\" href=\"https:\/\/discover.restek.com\/wp-content\/uploads\/800-00-001.pdf\">800-00-001<\/a><a href=\"https:\/\/discover.restek.com\/wp-content\/uploads\/800-00-001.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-44b5fb08-b036-4217-b308-aed780a03029\">Download<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Yes. Detailed instructions for conditioning SPE cartridges can be found\u00a0below. All SPE cartridges should be conditioned to wet and settle the bed, activate the packing materials, and remove any residual process materials (e.g., fines). As a general guide: Characteristics of Reversed- and Normal Phase SPE Packing Polarity Sample Matrix Conditioning Solution(s) Elution Solution C18, carbon&#8230;<\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[23],"tags":[2688,2708],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[],"hf_cat_post":[625],"ppma_author":[414],"class_list":["post-2940","post","type-post","status-publish","format-standard","hentry","category-faq","tag-faq-sample-preparation","tag-faq-solid-phase-extraction-spe"],"acf":[],"taxonomy_info":{"category":[{"value":23,"label":"FAQ"}],"post_tag":[{"value":2688,"label":"FAQ: Sample Preparation"},{"value":2708,"label":"FAQ: Solid Phase Extraction (SPE)"}]},"featured_image_src_large":false,"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/es\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":23,"name":"FAQ","slug":"faq","term_group":0,"term_taxonomy_id":23,"taxonomy":"category","description":"","parent":0,"count":271,"filter":"raw","cat_ID":23,"category_count":271,"category_description":"","cat_name":"FAQ","category_nicename":"faq","category_parent":0}],"tag_info":[{"term_id":2688,"name":"FAQ: Sample Preparation","slug":"faq-sample-preparation","term_group":0,"term_taxonomy_id":2688,"taxonomy":"post_tag","description":"Browse frequently asked questions for sample preparation products.","parent":0,"count":52,"filter":"raw"},{"term_id":2708,"name":"FAQ: Solid Phase Extraction (SPE)","slug":"faq-solid-phase-extraction-spe","term_group":0,"term_taxonomy_id":2708,"taxonomy":"post_tag","description":"Browse frequently asked questions for SPE products.","parent":0,"count":9,"filter":"raw"}],"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/2940","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/comments?post=2940"}],"version-history":[{"count":3,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/2940\/revisions"}],"predecessor-version":[{"id":70928,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/2940\/revisions\/70928"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/media?parent=2940"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/categories?post=2940"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/tags?post=2940"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/industries-application?post=2940"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/post-badge?post=2940"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/resource-type?post=2940"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/product-library?post=2940"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/resource-technique?post=2940"},{"taxonomy":"hf_cat_post","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/hf_cat_post?post=2940"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/ppma_author?post=2940"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}