{"id":3085,"date":"2025-07-30T21:19:30","date_gmt":"2025-07-30T21:19:30","guid":{"rendered":"https:\/\/discover.restek.com\/?p=3085"},"modified":"2025-12-09T00:10:55","modified_gmt":"2025-12-09T00:10:55","slug":"sometimes-when-my-original-method-contains-a-gradient-there-is-a-mismatch-between-the-number-of-steps-in-my-original-and-translation-methods-why-does-this-happen-a","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/es\/faq-de\/gnot5139\/sometimes-when-my-original-method-contains-a-gradient-there-is-a-mismatch-between-the-number-of-steps-in-my-original-and-translation-methods-why-does-this-happen-a","title":{"rendered":"Sometimes when my original method contains a gradient there is a mismatch between the number of steps in my \u201cOriginal\u201d and \u201cTranslation\u201d methods. Why does this happen and when should I expect it to happen?"},"content":{"rendered":"\n<p>This occurs because of a significant change to either column diameter or dwell volume (e.g., when transferring a method from one LC system to another LC system). In some instances, these differences can effectively act as a hold time at the initial %B. Depending on the scenario, an isocratic hold may be needed or not needed in the translated method for equivalence.<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Scenarios when an initial isocratic hold will be added to the translated method:\n<ul class=\"wp-block-list\">\n<li>The dwell volume of the original method is greater than the dwell volume of the translated method.<\/li>\n\n\n\n<li>Going from a narrow bore (ID) column to a wide bore (ID) column.<\/li>\n\n\n\n<li>Going from a shorter (length) column to a longer (length) column with the ID of the columns changing.<\/li>\n\n\n\n<li>Any combination of the above.<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li>Scenarios when an initial isocratic hold is not needed in the translated method:\n<ul class=\"wp-block-list\">\n<li>The dwell volume of the translated method is greater than the dwell volume of the original method.<\/li>\n\n\n\n<li>Going from a wide bore (ID) column to a narrow bore (ID) column.<\/li>\n\n\n\n<li>Going from a long (length) column to a shorter (length) column with the ID of the columns changing.<\/li>\n\n\n\n<li>Any combination of the above.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>This occurs because of a significant change to either column diameter or dwell volume (e.g., when transferring a method from one LC system to another LC system). In some instances, these differences can effectively act as a hold time at the initial %B. Depending on the scenario, an isocratic hold may be needed or not&#8230;<\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[802],"tags":[2685],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[],"hf_cat_post":[625],"ppma_author":[414],"class_list":["post-3085","post","type-post","status-publish","format-standard","hentry","category-faq-de","tag-faq-pro-ezlc-method-translator"],"acf":[],"taxonomy_info":{"category":[{"value":802,"label":"FAQ"}],"post_tag":[{"value":2685,"label":"FAQ: Pro EZLC Method Translator"}]},"featured_image_src_large":false,"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/es\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":802,"name":"FAQ","slug":"faq-de","term_group":0,"term_taxonomy_id":802,"taxonomy":"category","description":"","parent":0,"count":271,"filter":"raw","cat_ID":802,"category_count":271,"category_description":"","cat_name":"FAQ","category_nicename":"faq-de","category_parent":0}],"tag_info":[{"term_id":2685,"name":"FAQ: Pro EZLC Method Translator","slug":"faq-pro-ezlc-method-translator","term_group":0,"term_taxonomy_id":2685,"taxonomy":"post_tag","description":"Browse frequently asked questions about Restek's Pro <em>EZ<\/em>LC Method Translator.","parent":0,"count":12,"filter":"raw"}],"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/3085","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/comments?post=3085"}],"version-history":[{"count":2,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/3085\/revisions"}],"predecessor-version":[{"id":70970,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/3085\/revisions\/70970"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/media?parent=3085"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/categories?post=3085"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/tags?post=3085"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/industries-application?post=3085"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/post-badge?post=3085"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/resource-type?post=3085"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/product-library?post=3085"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/resource-technique?post=3085"},{"taxonomy":"hf_cat_post","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/hf_cat_post?post=3085"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/ppma_author?post=3085"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}