{"id":3258,"date":"2025-07-31T12:50:25","date_gmt":"2025-07-31T12:50:25","guid":{"rendered":"https:\/\/discover.restek.com\/?p=3258"},"modified":"2025-12-08T23:52:27","modified_gmt":"2025-12-08T23:52:27","slug":"how-much-sample-can-i-inject-on-my-lc-column-and-still-avoid-band-broadening","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/es\/faq\/gnot5051\/how-much-sample-can-i-inject-on-my-lc-column-and-still-avoid-band-broadening","title":{"rendered":"How much sample can I inject on my LC column and still avoid band broadening?"},"content":{"rendered":"\n

Injection volumes, as well as optimal flow rates, are limited by the size of the column. Ideally, if your sample is in the same solvent as your mobile phase, approximate volumes should range as follows:<\/p>\n\n\n\n

Column ID<\/strong><\/td>Volume (\u00b5L)<\/strong><\/td><\/tr>
2.1 mm (30 -100 mm length)<\/td>1-3<\/td><\/tr>
3.0-3.2 mm (50-150 mm length)<\/td>2-12<\/td><\/tr>
4.6 mm (50-250 mm length)<\/td>8-40<\/td><\/tr><\/tbody><\/table><\/figure>\n","protected":false},"excerpt":{"rendered":"

Injection volumes, as well as optimal flow rates, are limited by the size of the column. Ideally, if your sample is in the same solvent as your mobile phase, approximate volumes should range as follows: Column ID Volume (\u00b5L) 2.1 mm (30 -100 mm length) 1-3 3.0-3.2 mm (50-150 mm length) 2-12 4.6 mm (50-250…<\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[23],"tags":[2698,2682],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[],"hf_cat_post":[625],"ppma_author":[414],"class_list":["post-3258","post","type-post","status-publish","format-standard","hentry","category-faq","tag-faq-general-hplc","tag-faq-hplc-and-uhplc"],"acf":[],"taxonomy_info":{"category":[{"value":23,"label":"FAQ"}],"post_tag":[{"value":2698,"label":"FAQ: General HPLC"},{"value":2682,"label":"FAQ: HPLC and UHPLC"}]},"featured_image_src_large":false,"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/es\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":23,"name":"FAQ","slug":"faq","term_group":0,"term_taxonomy_id":23,"taxonomy":"category","description":"","parent":0,"count":271,"filter":"raw","cat_ID":23,"category_count":271,"category_description":"","cat_name":"FAQ","category_nicename":"faq","category_parent":0}],"tag_info":[{"term_id":2698,"name":"FAQ: General HPLC","slug":"faq-general-hplc","term_group":0,"term_taxonomy_id":2698,"taxonomy":"post_tag","description":"Browse general frequently asked questions for HPLC.","parent":0,"count":8,"filter":"raw"},{"term_id":2682,"name":"FAQ: HPLC and UHPLC","slug":"faq-hplc-and-uhplc","term_group":0,"term_taxonomy_id":2682,"taxonomy":"post_tag","description":"Browse frequently asked questions for HPLC and UHPLC products.","parent":0,"count":61,"filter":"raw"}],"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/3258","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/comments?post=3258"}],"version-history":[{"count":5,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/3258\/revisions"}],"predecessor-version":[{"id":72505,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/3258\/revisions\/72505"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/media?parent=3258"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/categories?post=3258"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/tags?post=3258"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/industries-application?post=3258"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/post-badge?post=3258"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/resource-type?post=3258"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/product-library?post=3258"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/resource-technique?post=3258"},{"taxonomy":"hf_cat_post","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/hf_cat_post?post=3258"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/ppma_author?post=3258"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}