{"id":42559,"date":"2022-01-03T05:00:00","date_gmt":"2022-01-03T05:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/optimizing-splitless-injection\/"},"modified":"2025-11-13T23:51:09","modified_gmt":"2025-11-13T23:51:09","slug":"optimizing-splitless-injection","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/es\/videos\/gnav3699\/optimizing-splitless-injection","title":{"rendered":"Optimizing Splitless Injection"},"content":{"rendered":"\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"Optimizing Splitless Injections\" width=\"720\" height=\"405\" src=\"https:\/\/www.youtube.com\/embed\/MtFlABnj8G4?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Description<\/h2>\n\n\n\n<p>Splitless injections excel at trace analyses, but to get to get the most out of a splitless analysis, it\u2019s important to optimize your system. But what should you optimize and what are the benefits?&nbsp;<\/p>\n\n\n\n<p>In this Restek Tip, we\u2019re going to show you how to optimize your splitless injections. We\u2019ll start with liner selection, before discussing the importance of the splitless hold time. Next, we\u2019ll focus on optimizing the inlet temperature, and finally, the initial oven temperature.&nbsp;<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Additional Resources<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.restek.com\/chromablography\/gc-inlet-liner-selection-part-i-splitless-liner-selection\" target=\"_blank\" rel=\"noopener\">GC Inlet Liner Selection: Splitless Liners<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/chromablography\/calculating-splitless-valve-time-for-splitless-injection-gc-with-the-new-ezgc-method-translator-and-flow-calculator---pesticides\" target=\"_blank\" rel=\"noopener\">Calculating Splitless Hold Time<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/articles\/how-to-choose-a-gc-inlet-liner\" target=\"_blank\" rel=\"noopener\">How to Choose a GC Inlet Liner<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/articles\/gc-inlet-maintenance-resteks-quick-reference-guide\" target=\"_blank\" rel=\"noopener\">GC Inlet Maintenance Guide<\/a><\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Restek Recommended Tools &amp; Parts<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.restek.com\/c\/1210\" target=\"_blank\" rel=\"noopener\">Restek Inlet Liners<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/p\/28500\" target=\"_blank\" rel=\"noopener\">Restek Electronic Leak Detector<\/a><\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Transcript<\/h2>\n\n\n\n<p>Splitless injection is an excellent technique for performing trace analyses, but to get the most out of a splitless analysis, it\u2019s important to optimize your system.&nbsp;&nbsp;<\/p>\n\n\n\n<p>But what should you optimize to get the best results?&nbsp;<\/p>\n\n\n\n<p>We are glad to back for this Restek Tip, and we\u2019re going to show you how to optimize your splitless injections to achieve high-quality chromatography. Let\u2019s start with liner selection.&nbsp;<\/p>\n\n\n\n<p>Choosing the right liner can drastically improve both analyte recovery and injection to injection reproducibility.&nbsp;&nbsp;<\/p>\n\n\n\n<p>For splitless analyses, you need a liner that maximizes homogenous vaporization, is inert, and has a tapered bottom.&nbsp;<\/p>\n\n\n\n<p>A single taper liner with wool, or a double taper cyclo liner are great choices.&nbsp;&nbsp;<\/p>\n\n\n\n<p>The extra surface area from the wool, or the corkscrew, aids in vaporizing and mixing your sample with the carrier gas. It also helps to trap non-volatile material, protecting the column.&nbsp;<\/p>\n\n\n\n<p>Liner deactivation is important for splitless injections. The longer inlet residence times could lead to analytes interacting with active sites within the liner and inlet. Selecting a well deactivated liner reduces adverse effects from these interactions.&nbsp;<\/p>\n\n\n\n<p>Lastly, the tapered design at the bottom of the liner helps to direct the sample towards the column and reduces contact with the base of the inlet where analyte breakdown can occur.&nbsp;<\/p>\n\n\n\n<p>Next, we\u2019ll optimize the inlet temperature. You want the temperature to be high enough to achieve sufficient vaporization, but low enough to not cause excessive activity.&nbsp;<\/p>\n\n\n\n<p>250\u00b0 Celsius is a good starting point, but you may need to adjust this temperature to achieve the overall best peak responses for your analysis.&nbsp;<\/p>\n\n\n\n<p>So now, how do we optimize our splitless hold time?&nbsp;<\/p>\n\n\n\n<p>Well, you want a hold time that\u2019s long enough to allow the complete vaporization and transfer of analytes to the column. But one that\u2019s too long can cause excessive solvent peak tailing, leading to interference with early eluting analytes.&nbsp;<\/p>\n\n\n\n<p>A good rule of thumb is for the carrier gas to sweep the total liner volume between 1 and a half and 2 times. Restek\u2019s&nbsp;<em>EZ<\/em>GC Flow Calculator can calculate the ideal hold time range for you.&nbsp;<\/p>\n\n\n\n<p>And finally: initial oven temperature.&nbsp;<\/p>\n\n\n\n<p>Since splitless mode has a slow flow-rate, band-broadening will often occur as analytes diffuse within the inlet.&nbsp;&nbsp;<\/p>\n\n\n\n<p>To help recondense these analytes at the head of the column you need a lower oven temperature, combined with an initial hold time that matches your splitless hold time.&nbsp;<\/p>\n\n\n\n<p>The exact temperature to use depends on the volatility of your analytes. If they are relatively volatile, the initial oven temperature should be below or close to the boiling point of the solvent. If they are significantly less volatile than the solvent, higher initial oven temperatures can be used.&nbsp;<\/p>\n\n\n\n<p>There you go. You\u2019ve optimized your splitless injection and are ready to start your analysis!&nbsp;<\/p>\n\n\n\n<p>If you have any questions, we\u2019re here to help! Leave a comment below or visit us at restek.com. And if you would like to see more videos like these, please like and subscribe.&nbsp;<\/p>\n\n\n\n<p>And, we are really looking forward to seeing you for our next Restek Tip.<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Splitless injections excel at trace analyses, but to get to get the most out of a splitless analysis, it\u2019s important to optimize your system. Watch our latest Restek Tip to learn about liner selection, splitless hold times, initial oven temperature, and more!<\/p>\n","protected":false},"author":11,"featured_media":41382,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[8],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[2372,2386],"resource-technique":[2294,2298],"hf_cat_post":[624],"ppma_author":[414],"class_list":["post-42559","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-videos","product-library-gas-chromatography-products","product-library-inlet-supplies","resource-technique-gas-chromatography-gc","resource-technique-injection-techniques"],"acf":[],"taxonomy_info":{"category":[{"value":8,"label":"Videos"}],"product-library":[{"value":2372,"label":"Gas Chromatography Products"},{"value":2386,"label":"Inlet Supplies"}],"resource-technique":[{"value":2294,"label":"Gas Chromatography (GC)"},{"value":2298,"label":"Injection Techniques"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-GNAV3699-1024x536.jpg",1024,536,true],"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/es\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":8,"name":"Videos","slug":"videos","term_group":0,"term_taxonomy_id":8,"taxonomy":"category","description":"","parent":0,"count":132,"filter":"raw","cat_ID":8,"category_count":132,"category_description":"","cat_name":"Videos","category_nicename":"videos","category_parent":0}],"tag_info":false,"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/42559","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/comments?post=42559"}],"version-history":[{"count":4,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/42559\/revisions"}],"predecessor-version":[{"id":64143,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/42559\/revisions\/64143"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/media\/41382"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/media?parent=42559"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/categories?post=42559"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/tags?post=42559"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/industries-application?post=42559"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/post-badge?post=42559"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/resource-type?post=42559"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/product-library?post=42559"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/resource-technique?post=42559"},{"taxonomy":"hf_cat_post","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/hf_cat_post?post=42559"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/ppma_author?post=42559"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}