{"id":42587,"date":"2023-09-25T04:00:00","date_gmt":"2023-09-25T04:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/choosing-your-lc-particle\/"},"modified":"2025-11-26T17:51:47","modified_gmt":"2025-11-26T17:51:47","slug":"choosing-your-lc-particle","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/es\/videos\/gnav4891\/choosing-your-lc-particle","title":{"rendered":"Choosing Your LC Particle"},"content":{"rendered":"\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"Choosing Your LC Particle\" width=\"720\" height=\"405\" src=\"https:\/\/www.youtube.com\/embed\/cPdg8b0NbV0?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Description<\/h2>\n\n\n\n<p>We have a lot of options when choosing a column for our liquid chromatography&nbsp;(LC)&nbsp;application. In addition to the stationary phase and the column dimension, we also need to pick the perfect particle. We cover the differences between superficially porous particles and fully porous particles, pore size, and more to give you the information you need to make the right choice.&nbsp;&nbsp;<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Additional Resources<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.restek.com\/articles\/frequently-asked-questions-hplc-and-uhplc\" target=\"_blank\" rel=\"noopener\">HPLC and UHPLC FAQ<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/articles\/the-effects-of-lc-particle-choice-on-column-performance-raptor-1.8-m-superficially-porous-particles-spp-for-uhplc-analyses\" target=\"_blank\" rel=\"noopener\">Effects of LC Particle Choice on Column Performance<\/a><\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Restek Recommended Tools &amp; Parts<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.restek.com\/c\/1119\" target=\"_blank\" rel=\"noopener\">LC Columns<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/c\/1121\" target=\"_blank\" rel=\"noopener\">LC Tubing and Accessories<\/a><\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Transcript<\/h2>\n\n\n\n<p>Fully porous or superficially porous? 1.8&nbsp;or&nbsp;2.7 \u00b5m?&nbsp; 90 or 300 \u00c5? These are only a handful of the choices that are available when you are choosing your LC particle. Superficially porous particles, such as Raptor, feature a solid, impermeable core enveloped by a thin, porous layer of silica that decreases the diffusion path and reduces peak dispersion. This, in combination with other unique properties resulting from the solid core, allows the use of a 2.7 \u00b5m superficially porous particle to achieve the performance of a sub-2 \u00b5m fully porous particle while generating less backpressure. If that\u2019s the case, why even consider fully porous particles? These particles have more surface area per gram,&nbsp;allowing both additional retention for poorly retained compounds and the ability to inject more mass on column compared to superficially porous particles.<\/p>\n\n\n\n<p>Let\u2019s also consider particle size. For the same column dimension, if the particle size is reduced by half, the plate number doubles.&nbsp; However, backpressure also increases by a factor of four. Keeping this in mind, the pressure capabilities of your instrumentation should align with your choice in particle size. Pore size is often overlooked when selecting an LC particle. Small-pore particles have pore sizes ranging from 60\u2013150 \u00c5 and perform well during separations of small molecules that are less than approximately 1,000 Da. Large-pore particles tend to be used for separation of larger molecules such as proteins. As a general rule, the pore size should be at least three times the size of hydrodynamic diameter of the molecule for easy access to the pores.<\/p>\n\n\n\n<p>Before choosing the particle for your next analytical column, consider the size of your target&nbsp;analytes&nbsp;and whether you need speed, backpressure restrictions, sample capacity, or retention in order to effectively pair your column with your analysis.<\/p>\n\n\n\n<p>If you enjoy these videos, please like, share,&nbsp;and subscribe. You can post your ideas for a future tech tip in the comments below.&nbsp;Thanks for watching,&nbsp;and we\u2019ll see you next time.<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Pick the perfect particle! In this Restek Tip, we\u2019ll talk about how to choose the right particle for your liquid chromatography column.<\/p>\n","protected":false},"author":11,"featured_media":41454,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[8],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[567],"product-library":[2391,2373],"resource-technique":[2299],"hf_cat_post":[624],"ppma_author":[414],"class_list":["post-42587","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-videos","resource-type-selection-guides","product-library-lc-columns","product-library-liquid-chromatography-products","resource-technique-liquid-chromatography"],"acf":[],"taxonomy_info":{"category":[{"value":8,"label":"Videos"}],"resource-type":[{"value":567,"label":"Selection Guides"}],"product-library":[{"value":2391,"label":"LC Columns"},{"value":2373,"label":"Liquid Chromatography Products"}],"resource-technique":[{"value":2299,"label":"Liquid Chromatography"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-GNAV4891-1024x536.jpg",1024,536,true],"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/es\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":8,"name":"Videos","slug":"videos","term_group":0,"term_taxonomy_id":8,"taxonomy":"category","description":"","parent":0,"count":132,"filter":"raw","cat_ID":8,"category_count":132,"category_description":"","cat_name":"Videos","category_nicename":"videos","category_parent":0}],"tag_info":false,"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/42587","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/comments?post=42587"}],"version-history":[{"count":3,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/42587\/revisions"}],"predecessor-version":[{"id":64130,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/42587\/revisions\/64130"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/media\/41454"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/media?parent=42587"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/categories?post=42587"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/tags?post=42587"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/industries-application?post=42587"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/post-badge?post=42587"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/resource-type?post=42587"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/product-library?post=42587"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/resource-technique?post=42587"},{"taxonomy":"hf_cat_post","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/hf_cat_post?post=42587"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/ppma_author?post=42587"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}