{"id":43381,"date":"2020-10-13T00:00:00","date_gmt":"2020-10-13T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/improved-gc-analysis-of-derivatized-amphetamines\/"},"modified":"2025-12-09T16:53:12","modified_gmt":"2025-12-09T16:53:12","slug":"improved-gc-analysis-of-derivatized-amphetamines","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/es\/articles\/cfss2681\/improved-gc-analysis-of-derivatized-amphetamines","title":{"rendered":"Improved GC Analysis of Derivatized Amphetamines"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Ideal selectivity for GC analysis of amphetamines.<\/li>\n\n\n\n<li>Exceptional inertness means good peak shape\/response and better sensitivity.<\/li>\n\n\n\n<li>Robust, low-bleed phase results in longer column lifetime.<\/li>\n<\/ul>\n\n\n\n<p>Many drug assays, such as GC analysis of amphetamines, require that compounds be derivatized prior to analysis. Derivatization allows compounds that are not otherwise amenable to GC to be analyzed by this technique, and it also helps to produce unique, high molecular weight fragments that assist with GC-MS quantitation. While derivatization has its advantages, derivatization reagents and their byproducts are extremely harsh and can reduce GC column lifetime by damaging the stationary phase. Phase damage usually manifests as increased bleed and tailing of active compounds. The GC analysis of derivatized amphetamines shown below illustrates the low bleed levels, good peak responses, and symmetrical peak shapes that can be obtained by using a highly inert Rxi-5Sil MS column.<\/p>\n\n\n\n<p>The unique Rxi-5Sil MS stationary phase, with its embedded arylene groups, provides a more rigid structure that is less likely to be damaged by drug derivatization reagents or their byproducts. Internal lifetime testing at Restek has demonstrated that after 400 injections of HFAA, a reagent used to derivatize amphetamines and other drugs prior to analysis, no changes in bleed or inertness were observed. The enhanced stability of Rxi-5Sil MS columns reduces phase bleed, resulting in longer column lifetimes and improved chromatographic performance for the GC analysis of derivatized amphetamines and other drugs of abuse with sensitive mass spectrometry detectors.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/gc_cf1144.png\" alt=\"Derivatized Amphetamines (500 ng\/mL) on Rxi-5Sil MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> GC_CF1144<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Amphetamine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/300-62-9\/Amphetamine\" target=\"_blank\" rel=\"noopener\">Amphetamine<\/a><\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Methamphetamine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/51-57-0\/Methamphetamine\" target=\"_blank\" rel=\"noopener\">Methamphetamine<\/a><\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for MDA\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/4764-17-4\/MDA\" target=\"_blank\" rel=\"noopener\">MDA<\/a><\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for MDMA\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/42542-10-9\/MDMA\" target=\"_blank\" rel=\"noopener\">MDMA<\/a><\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\">MDEA<\/td><\/tr>\n<\/tbody><\/table><div class=\"peaknotes\">c = contaminant<\/div><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Rxi-5Sil MS, 30 m, 0.25 mm ID, 0.25 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/13623?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_CF1144\" rel=\"noopener\">cat.# 13623<\/a>)<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Butyl chloride<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>500 ng\/mL HFAA derivatives<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Injection<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>1 \u00b5L splitless (hold 1  min)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Liner:<\/th><td>3.5 mm splitless taper w\/wool<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Temp.:<\/th><td>250 \u00b0C<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Purge Flow:<\/th><td>28 mL\/min<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\" colspan=\"2\">Oven<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Oven Temp.:<\/th><td>75 &#176;C to 300 &#176;C at 15 &#176;C\/min<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Carrier Gas<\/th><td>He, constant linear velocity<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Linear Velocity:<\/th><td>45 cm\/sec, 13.5 psi, 93.1 kPa @ 75 \u00b0C<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>Scan<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Transfer Line Temp.:<\/th><td>250 \u00b0C<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Analyzer Type:<\/th><td>Quadrupole <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Source Temp.:<\/th><td>200 \u00b0C<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Electron Energy:<\/th><td>70 eV<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Solvent Delay Time:<\/th><td>4  min<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Tune Type:<\/th><td>PFTBA <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ionization Mode:<\/th><td>EI <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Scan Range:<\/th><td>40-300 amu<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Scan Rate:<\/th><td>5 scans\/sec<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Shimadzu 2010 GC &amp; QP2010+ MS<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Liner cat.# 22286-200.1 was used to produce this chromatogram, but it has since been discontinued. We recommend cat.# 23336 as an alternative. For assistance choosing a replacement for this application, contact Restek Technical Service or your local Restek representative.<br \/><\/td><\/tr><\/table><\/div><\/div><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>GC analysis of derivatized amphetamines and other drugs of abuse can be improved by using Rxi-5Sil MS columns. Robust and highly inert, these columns withstand harsh conditions, such as exposure to the derivatization reagents. High inertness and enhanced stability result in good peak shape and response, low phase bleed, and longer column lifetimes.<\/p>\n","protected":false},"author":11,"featured_media":6475,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[7],"tags":[],"industries-application":[2153,2156,2170,2171],"post-badge":[],"resource-type":[2623],"product-library":[2372,2384,2386],"resource-technique":[2294,2301],"hf_cat_post":[650,2624],"ppma_author":[414],"class_list":["post-43381","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles","industries-application-clinical","industries-application-clinical-toxicology","industries-application-forensics","industries-application-forensics-toxicology","resource-type-featured-application","product-library-gas-chromatography-products","product-library-gc-columns","product-library-inlet-supplies","resource-technique-gas-chromatography-gc","resource-technique-mass-spectrometry-ms"],"acf":[],"taxonomy_info":{"category":[{"value":7,"label":"Articles"}],"industries-application":[{"value":2153,"label":"Clinical"},{"value":2156,"label":"Clinical 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