{"id":43382,"date":"2020-12-21T23:00:00","date_gmt":"2020-12-21T23:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/raptor-lc-columns-stationary-phase-etgets\/"},"modified":"2026-01-03T14:29:54","modified_gmt":"2026-01-03T14:29:54","slug":"raptor-lc-columns-stationary-phase-etgets","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/es\/articles-ja\/cfss2741\/raptor-lc-columns-stationary-phase-etgets","title":{"rendered":"Raptor EtG\/EtS: All Matrix Interferences Resolved"},"content":{"rendered":"\n<p>How often are matrix interferences affecting your EtG\/EtS analysis? Restek\u2019s EtG\/EtS solution consistently resolves analytes away from matrix interferences in a fast, 4-minute, dilute-and-shoot method.<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Proven performance for accurate, reliable ethyl glucuronide (EtG) and ethyl sulfate (EtS) analysis.<\/li>\n\n\n\n<li>Strong retention consistently resolves analytes from matrix interferences.<\/li>\n\n\n\n<li>Long column lifetime ensures consistent performance injection after injection.<\/li>\n\n\n\n<li>Fast, 4-minute, dilute-and-shoot LC-MS\/MS analysis supports high sample throughput.<\/li>\n<\/ul>\n\n\n\n<p>Switch to a Raptor EtG\/EtS column when you need consistent resolution of EtG and EtS from matrix interferences and long column lifetimes.<\/p>\n\n\n\n<p>Order yours today at <a href=\"articles\/raptor-lc-columns&quot;\">www.restek.com\/raptor<\/a><\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h2 class=\"wp-block-heading\">Column Description:<\/h2>\n\n\n\n<div style=\"height:40px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<style>.kb-image43382_4d0787-7b.kb-image-is-ratio-size, .kb-image43382_4d0787-7b .kb-image-is-ratio-size{max-width:225px;width:100%;}.wp-block-kadence-column > .kt-inside-inner-col > .kb-image43382_4d0787-7b.kb-image-is-ratio-size, .wp-block-kadence-column > .kt-inside-inner-col > .kb-image43382_4d0787-7b .kb-image-is-ratio-size{align-self:unset;}.kb-image43382_4d0787-7b figure{max-width:225px;}.kb-image43382_4d0787-7b .image-is-svg, .kb-image43382_4d0787-7b .image-is-svg img{width:100%;}.kb-image43382_4d0787-7b .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43382_4d0787-7b\"><figure class=\"aligncenter size-medium_large\"><img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"1760\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/article-ligand-cfss2741b-01-1-768x1760.jpg\" alt=\"\" class=\"kb-img wp-image-61639\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/article-ligand-cfss2741b-01-1-768x1760.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-ligand-cfss2741b-01-1-131x300.jpg 131w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-ligand-cfss2741b-01-1-447x1024.jpg 447w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-ligand-cfss2741b-01-1-670x1536.jpg 670w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-ligand-cfss2741b-01-1-894x2048.jpg 894w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-ligand-cfss2741b-01-1-scaled.jpg 1117w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/figure><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<div style=\"height:40px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading\">Pore Size:<\/h4>\n\n\n\n<p>90 \u00c5<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Particle:<\/h4>\n\n\n\n<p>2.7 \u03bcm superficially porous particle (SPP or \u201ccore-shell\u201d particle) silica<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Surface Area:<\/h4>\n\n\n\n<p>130 m<sup>2<\/sup>\/g<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">End-Cap:<\/h4>\n\n\n\n<p>Proprietary<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Carbon Load:<\/h4>\n\n\n\n<p>Proprietary<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">USP Phase Code:<\/h4>\n\n\n\n<p>NA<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Phase Category:<\/h4>\n\n\n\n<p>Proprietary<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Ligand Type:<\/h4>\n\n\n\n<p>Proprietary<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"432\" height=\"272\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/article-profile-cfss2741b-01a.jpg\" alt=\"\" class=\"wp-image-12232\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/article-profile-cfss2741b-01a.jpg 432w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-profile-cfss2741b-01a-300x189.jpg 300w\" sizes=\"auto, (max-width: 432px) 100vw, 432px\" \/><\/figure>\n\n\n\n<div style=\"height:40px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading\">Recommended Usage:<\/h4>\n\n\n\n<p>pH Range: 2.0\u20138.0<br>Maximum Temperature: 40 \u00b0C<br>Maximum Pressure:&nbsp;600 bar\/8700 psi<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Properties:<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Resolution of EtG and EtS from matrix interferences.<\/li>\n\n\n\n<li>Increased retention of EtG and EtS compared<br>to traditional phases.<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">Switch to a Raptor EtG\/EtS when:<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Other columns can\u2019t resolve EtG and EtS from matrix components.<\/li>\n\n\n\n<li>You need high-throughput EtG\/EtS analysis.<\/li>\n\n\n\n<li>Low-level detection limits are desired.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Definitive Results for Alcohol Monitoring<\/h2>\n\n\n\n<p>Restek has developed a new Raptor column that significantly improves EtG and EtS analysis for alcohol monitoring programs. The Raptor EtG\/EtS column consistently retains and resolves these important biomarkers from matrix interferences, providing accurate, reliable results injection after injection (Figure 1). Dependable performance, a fast 4-minute analysis, and long column lifetime make this column ideal for high-throughput labs. Paired with our quality reference standards and the optimized, verified method shown below, Restek\u2019s Raptor EtG\/EtS column is part of a complete solution that will help testing labs save time and increase certainty.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 1:<\/strong>\u00a0Rugged Raptor EtG\/EtS columns provide complete resolution and consistent results even after 1000 injections, allowing more samples to be analyzed between column changes.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0706.png\" alt=\"1,000 Injection Lifetime: EtG and EtS in Urine on Raptor EtG\/EtS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_CF0706<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ethyl-\u03b2-D-glucuronide-d5\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1135070-98-2\/Ethyl-\u03b2-D-glucuronide-d5\" target=\"_blank\" rel=\"noopener\">Ethyl-\u03b2-D-glucuronide-d5<\/a><\/td><td class=\"oth\">225.9<\/td><td class=\"oth\">84.9<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ethyl-\u03b2-D-glucuronide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/17685-04-0\/Ethyl-\u03b2-D-glucuronide\" target=\"_blank\" rel=\"noopener\">Ethyl-\u03b2-D-glucuronide<\/a><\/td><td class=\"oth\">220.8<\/td><td class=\"oth\">84.9<\/td><td class=\"oth\">74.8<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ethyl sulfate-d5\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1329611-05-3\/Ethyl sulfate-d5\" target=\"_blank\" rel=\"noopener\">Ethyl sulfate-d5<\/a><\/td><td class=\"oth\">129.7<\/td><td class=\"oth\">97.7<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ethyl sulfate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/64-67-5\/Ethyl sulfate\" target=\"_blank\" rel=\"noopener\">Ethyl sulfate<\/a><\/td><td class=\"oth\">124.7<\/td><td class=\"oth\">96.8<\/td><td class=\"oth\">79.7<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor EtG\/EtS  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9325A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0706\" rel=\"noopener\">cat.# 9325A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>UltraShield UHPLC precolumn filter, 0.2 \u00b5m frit (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/25809?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0706\" rel=\"noopener\">cat.# 25809<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>0.1% Formic acid in water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>  A 500 ng\/mL QC sample was prepared in urine. 50 \u00b5L of the sample was diluted with 950 \u00b5L of a working internal standard (25 ng\/mL EtS-d5\/100 ng\/mL EtG-d5 in 0.1% formic acid in water). The sample was vortexed at 3500 rpm for 10 seconds to mix. The sample was then centrifuged at 3000 rpm for 5 minutes at 10 \u00b0C. The autosampler needle was adjusted to inject from the supernatant.<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.1% Formic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>0.1% Formic acid in acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>2.50<\/td><td>0.5<\/td><td>65<\/td><td>35<\/td><\/tr><tr><td>2.51<\/td><td>0.5<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>4.00<\/td><td>0.5<\/td><td>95<\/td><td>5<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>HPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td><b>Reference Standards<\/b><br \/>Ethyl-\u03b2-D-glucuronide (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/34101?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0706\" rel=\"noopener\">cat.# 34101<\/a>)<br \/>Ethyl-\u03b2-D-glucuronide-d5 (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/34102?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0706\" rel=\"noopener\">cat.# 34102<\/a>)<br \/>Ethyl sulfate sodium salt (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/34103?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0706\" rel=\"noopener\">cat.# 34103<\/a>)<br \/>Ethyl sulfate-d5 sodium salt (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/34104?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0706\" rel=\"noopener\">cat.# 34104<\/a>)<br \/><\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0706.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Experience <em>Selectivity Accelerated<\/em>. Order the Raptor EtG\/EtS LC Column today.<\/h2>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">Related Products<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9325A12\">                                Catalog No. 9325A12                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Raptor EtG\/EtS, 2.7 \u00b5m, 100 x 2.1 mm LC Column<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n             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target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/34101\">View Product<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/34102\">                                Catalog No. 34102                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Ethyl-beta-D-Glucuronide-d5 (EtG-d5) Standard, 1000 \u00b5g\/mL, Methanol, 1 mL\/ampul<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/34102\">View Product<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/34103\">                                Catalog No. 34103                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Ethyl Sulfate Sodium Salt (EtS) Standard, 1000 \u00b5g\/mL, Methanol, 1 mL\/ampul<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/34103\">View Product<\/a>\n                                                    <\/div>\n                    <\/div>\n                            <\/div>\n        <\/div>\n        \n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Restek\u2019s EtG\/EtS solution consistently resolves analytes away from matrix interferences in a fast, 4-minute, dilute-and-shoot method.<\/p>\n","protected":false},"author":11,"featured_media":6481,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[789],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[2571,2553],"resource-technique":[2343,2365],"ppma_author":[414],"class_list":["post-43382","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles-ja","product-library-lc-columns-ja","product-library-liquid-chromatography-products-ja","resource-technique-liquid-chromatography-ja","resource-technique-ms-ms-ja"],"acf":[],"taxonomy_info":{"category":[{"value":789,"label":"\u6280\u8853\u8cc7\u6599"}],"product-library":[{"value":2571,"label":"LC 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