{"id":43517,"date":"2020-10-14T14:30:00","date_gmt":"2020-10-14T14:30:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/4-minute-bisphenol-a-bpa-analysis-increases-sample-throughput\/"},"modified":"2025-12-31T13:40:29","modified_gmt":"2025-12-31T13:40:29","slug":"4-minute-bisphenol-a-bpa-analysis-increases-sample-throughput","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/es\/artikel\/evss2395\/4-minute-bisphenol-a-bpa-analysis-increases-sample-throughput","title":{"rendered":"4-Minute Bisphenol A (BPA) Analysis Increases Sample Throughput"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Bisphenol A elutes in less than 2 minutes, increasing lab productivity.<\/li>\n\n\n\n<li>Narrow, symmetrical peak shape means easy, accurate quantitation.<\/li>\n\n\n\n<li>Alternate selectivity compared to a C18 allows easier identification in difficult matrices.<\/li>\n\n\n\n<li>Fast separation regardless of instrument platform\u2014compatible with both HPLC and UHPLC systems.<\/li>\n<\/ul>\n\n\n\n<p>Bisphenol A (BPA) is a high production volume industrial chemical used to make polycarbonate plastics and epoxy resins that are used in many industries. BPA is released into the environment through industrial effluents and can also leach from packaging materials into food and beverage products. Although BPA concentrations are often low, repeated daily exposure can result in high cumulative doses. Interest in BPA analysis is growing as bisphenol A is an endocrine disruptor that can mimic estrogen, thus disrupting normal hormone levels and causing a significant impact on health. It is also potentially linked to obesity, diabetes, ADHD, and other health conditions. BPA HPLC analysis can be done using a C18 LC column, but analysis times can be long and peak shape often is not ideal.<\/p>\n\n\n\n<p>Restek\u2019s Raptor Biphenyl column is a better alternative than a C18 column for BPA analysis. As shown in the chromatogram below, a Raptor Biphenyl column provides a fast and selective means to isolate and quantitate bisphenol A by LC-MS\/MS. It generates narrow, symmetrical peaks in a fast 4-minute analysis time, with bisphenol A eluting in less than 2 minutes. In addition, Raptor Biphenyl columns can be used with both conventional HPLC and UHPLC instruments. For BPA analysis, Raptor Biphenyl columns are recommended because they provide better peak shapes with short analysis times that allow labs to increase sample throughput and overall productivity.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0559.png\" alt=\"Analysis of Bisphenol A on Raptor Biphenyl by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_GN0559<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Quantifer Product Ion<\/th><th style=\"text-align: center;width: 75px\">Qualifier Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol A (BPA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/80-05-7\/Bisphenol A\" target=\"_blank\" rel=\"noopener\">Bisphenol A (BPA)<\/a><\/td><td class=\"oth\">1.27<\/td><td class=\"oth\">227.1<\/td><td class=\"oth\">212.1<\/td><td class=\"oth\">133.1<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Biphenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309552?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_GN0559\" rel=\"noopener\">cat.# 9309552<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>5 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>50:50 Mobile phase A:mobile phase B<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>100 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>5 mM Ammonium acetate in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>75<\/td><td>25<\/td><\/tr><tr><td>2.00<\/td><td>0.5<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>2.01<\/td><td>0.5<\/td><td>75<\/td><td>25<\/td><\/tr><tr><td>4.00<\/td><td>0.5<\/td><td>75<\/td><td>25<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>HPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0559.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">Products Mentioned<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9309552\">                                Catalog No. 9309552                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Raptor Biphenyl, 5 \u00b5m, 50 x 2.1 mm HPLC Column<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9309552\">View Product<\/a>\n                                                    <\/div>\n                    <\/div>\n                            <\/div>\n        <\/div>\n        \n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>A Raptor Biphenyl column is a better choice for BPA analysis than a conventional C18 column. On a Raptor Biphenyl column, narrow, symmetrical bisphenol A peaks elute in less than 2 minutes, providing a fast and selective means to isolate and quantitate bisphenol A by LC-MS\/MS.<\/p>\n","protected":false},"author":11,"featured_media":70525,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[787],"tags":[],"industries-application":[2161,2167,2169],"post-badge":[],"resource-type":[2623],"product-library":[2391,2373],"resource-technique":[2299,2302],"hf_cat_post":[650,2624],"ppma_author":[414],"class_list":["post-43517","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-artikel","industries-application-environmental","industries-application-food-beverage","industries-application-food-safety","resource-type-featured-application","product-library-lc-columns","product-library-liquid-chromatography-products","resource-technique-liquid-chromatography","resource-technique-ms-ms"],"acf":[],"taxonomy_info":{"category":[{"value":787,"label":"Artikel"}],"industries-application":[{"value":2161,"label":"Environmental"},{"value":2167,"label":"Food &amp; 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