{"id":43519,"date":"2020-11-05T13:30:00","date_gmt":"2020-11-05T13:30:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/improve-eph-testing-accuracy-and-efficiency-with-resprep-eph-fractionation-spe-cartridges\/"},"modified":"2025-12-09T16:10:03","modified_gmt":"2025-12-09T16:10:03","slug":"improve-eph-testing-accuracy-and-efficiency-with-resprep-eph-fractionation-spe-cartridges","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/es\/articles\/evss2574\/improve-eph-testing-accuracy-and-efficiency-with-resprep-eph-fractionation-spe-cartridges","title":{"rendered":"Improve EPH Testing Accuracy and Efficiency with Resprep EPH Fractionation SPE Cartridges"},"content":{"rendered":"\n<ul id=\"eph\" class=\"wp-block-list\">\n<li>Method-specific performance for extractable petroleum hydrocarbon (EPH) analysis of soil and water samples.<\/li>\n\n\n\n<li>Complete separation of aliphatic and aromatic compounds into distinct fractions.<\/li>\n\n\n\n<li>Guaranteed background level under the strict reporting limits of MA and NJ EPH methods.<\/li>\n\n\n\n<li>Superior lot-to-lot reproducibility and storage stability ensured by rigorous QC testing and moisture-resistant packaging.<\/li>\n\n\n\n<li>Available in two formats: 5 g bed for higher loading capacity and 2 g bed for automated systems, time-saving, and solvent reduction.<\/li>\n<\/ul>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 1:<\/strong>\u00a0Resprep EPH SPE cartridges offer consistent and complete separation between aliphatic and aromatic compounds from one lot to the next. A representative subset of compounds is shown here (40+ compounds were analyzed in total) to clearly illustrate the separation between fractions and the lack of breakthrough in every lot we produce.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<style>.kb-image43519_5b018b-36 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43519_5b018b-36\"><figure class=\"aligncenter size-medium_large\"><img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"1833\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVSS2574B-01-768x1833.jpg\" alt=\"\" class=\"kb-img wp-image-19726\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVSS2574B-01-768x1833.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVSS2574B-01-126x300.jpg 126w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVSS2574B-01-429x1024.jpg 429w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVSS2574B-01-644x1536.jpg 644w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVSS2574B-01-858x2048.jpg 858w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVSS2574B-01-scaled.jpg 1073w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/figure><\/div>\n\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 2:<\/strong>\u00a0Restek guarantees a background level in each fraction of less than half the strict reporting limits of MA and NJ EPH methods. (Tables give average total extracted background for both formats; example chromatography used 5 g cartridge.)<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<p><\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Aliphatic Fraction<\/h3>\n\n\n\n<figure class=\"wp-block-table\">\n<table class=\"has-fixed-layout\">\n<tbody>\n<tr style=\"text-align: center; background-color: #e1effa;\">\n<td rowspan=\"2\"><strong>Format<\/strong><\/td>\n<td rowspan=\"2\"><strong>Lot #<\/strong><\/td>\n<td colspan=\"2\"><strong>Average ppm (n = 3)<\/strong><\/td>\n<\/tr>\n<tr style=\"text-align: center; background-color: #e1effa;\">\n<td><strong>Region 1<br \/>(C9 &#8211; C18)<\/strong><\/td>\n<td><strong>Region 2<br \/>(C18 &#8211; C36)<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" rowspan=\"2\">2 g<\/td>\n<td style=\"text-align: center;\">1<\/td>\n<td style=\"text-align: center;\">3.2<\/td>\n<td style=\"text-align: center;\">2.5<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\">2<\/td>\n<td style=\"text-align: center;\">3.7<\/td>\n<td style=\"text-align: center;\">2.9<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" rowspan=\"2\">5 g<\/td>\n<td style=\"text-align: center;\">1<\/td>\n<td style=\"text-align: center;\">13.2<\/td>\n<td style=\"text-align: center;\">17.3<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\">2<\/td>\n<td style=\"text-align: center;\">11.3<\/td>\n<td style=\"text-align: center;\">16.4<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n\n\n\n<p>Restek aliphatic quality limits: &lt;35 ppm each region<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/gc_ev1437.png\" alt=\"EPH Silica Cartridge Background Extraction Aliphatic Fraction on Rxi-5ms\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> GC_EV1437<\/p><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/gc_ev1437.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<h3 class=\"wp-block-heading\">Aromatic Fraction<\/h3>\n\n\n\n<figure class=\"wp-block-table\">\n<table class=\"has-fixed-layout\">\n<tbody>\n<tr style=\"text-align: center; background-color: #e1effa;\">\n<td><strong>Format<\/strong><\/td>\n<td><strong>Lot #<\/strong><\/td>\n<td><strong>Average ppm<br \/>C9-C36 (n = 3)<\/strong><\/td>\n<\/tr>\n<tr style=\"text-align: center;\">\n<td rowspan=\"2\">2 g<\/td>\n<td>1<\/td>\n<td>9.5<\/td>\n<\/tr>\n<tr style=\"text-align: center;\">\n<td>2<\/td>\n<td>9.5<\/td>\n<\/tr>\n<tr style=\"text-align: center;\">\n<td rowspan=\"2\">5 g<\/td>\n<td>1<\/td>\n<td>34.2<\/td>\n<\/tr>\n<tr style=\"text-align: center;\">\n<td>2<\/td>\n<td>35.6<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n\n\n\n<p>Restek aromatic quality limits: &lt;50 ppm<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/gc_ev1438.png\" alt=\"EPH Silica Cartridge Background Extraction Aromatic Fraction on Rxi-5ms\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> GC_EV1438<\/p><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/gc_ev1438.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<p><strong>For 5 g Cartridges:<\/strong>&nbsp;Column: Rxi-5ms, 30 m, 0.32 mm ID, 0.25 \u03bcm (cat.# 13424); Sample: MA EPH surrogate spike mix (cat.# 31479); Conc.: 5 \u03bcg\/mL; Injection Inj. Vol.: 1 \u03bcL splitless (hold 1 min); Liner: Restek Premium 4.0 mm ID cyclo double taper inlet liner (cat.# 23310); Inj. Temp.: 300 \u00b0C; Purge Flow: 50 mL\/min; Oven Temp.: 40 \u00b0C (hold 1 min) to 300 \u00b0C at 12 \u00b0C\/min to 330 \u00b0C at 6 \u00b0C\/min; Carrier Gas: He, constant flow; Flow Rate.: 2 mL\/min; Linear Velocity: 41.0 cm\/sec @ 40 \u00b0C; Dead Time: 1.12 min @ 40 \u00b0C; Detector: FID @ 330 \u00b0C; Make-up Gas Flow Rate: 45 mL\/min; Make-up Gas Type: N\u2082 Hydrogen flow: 40 mL\/min; Air flow: 450 mL\/min; Data Rate: 50 Hz Instrument: Agilent 7890B GC; Notes: The area of the surrogate peak was excluded from the calculated total extracted background value.<\/p>\n\n\n\n<p><strong>For 2 g Cartridges:<\/strong>&nbsp;Column: Rxi-5ms, 30 m, 0.25 mm ID, 0.25 \u03bcm (cat.# 13423); Sample: MA EPH surrogate spike mix (cat.# 31479); Conc.: 5 \u03bcg\/mL; Injection Inj. Vol.: 1 \u03bcL splitless; Liner: Topaz single taper inlet liner with wool (cat.# 23303); Inj. Temp.: 250 \u00b0C; Purge Flow: 50 mL\/min; Oven Temp.: 40 \u00b0C (hold 1 min) to 300 \u00b0C at 12 \u00b0C\/min to 330 \u00b0C at 6 \u00b0C\/min; Carrier Gas: He, constant flow; Flow Rate.: 2 mL\/min; Linear Velocity: 41.0 cm\/sec @ 40 \u00b0C; Dead Time: 1.12 min @ 40 \u00b0C; Detector: FID @ 330 \u00b0C; Make-up Gas Flow Rate: 45 mL\/min; Make-up Gas Type: N\u2082 Hydrogen flow: 40 mL\/min; Air flow: 450 mL\/min; Data Rate: 50 Hz Instrument: Agilent 7890B GC; Notes: The area of the surrogate peak was excluded from the calculated total extracted background value.<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-47c7f9e9 wp-block-columns-is-layout-flex\" style=\"background-color:#00b5cb;padding-top:10px;padding-right:20px;padding-bottom:10px;padding-left:20px\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<p class=\"has-theme-palette-9-color has-text-color has-link-color wp-elements-21b3d9cc5e1b62525ae7e82fc08bfb08\"><strong>No background, no problems.<\/strong><\/p>\n\n\n\n<p class=\"has-theme-palette-9-color has-text-color has-link-color wp-elements-51e3b673b30b459ffe379b9dbe24c54e\">Resprep EPH cartridges are guaranteed clean\u2014we certify our background levels will always meet method requirements.<\/p>\n<\/div>\n<\/div>\n\n<\/div><\/div><\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Same High-Performance Silica Now Available in Two Formats<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Use 5 g bed for higher loading capacity.<\/li>\n\n\n\n<li>Use 2 g bed for automated systems, time-saving, and solvent reduction.<\/li>\n<\/ul>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 3:<\/strong>\u00a0Performance indicator compounds demonstrate the elution profiles of 5 g and 2 g cartridges packed with the same silica lot so you know exactly where target analytes will elute.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<style>.kb-image43519_f10c0c-a4 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43519_f10c0c-a4\"><figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"651\" height=\"1024\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVSS2574B-02-651x1024.jpg\" alt=\"\" class=\"kb-img wp-image-19732\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVSS2574B-02-651x1024.jpg 651w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVSS2574B-02-191x300.jpg 191w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVSS2574B-02-768x1208.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVSS2574B-02-976x1536.jpg 976w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVSS2574B-02-1302x2048.jpg 1302w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVSS2574B-02-scaled.jpg 1627w\" sizes=\"auto, (max-width: 651px) 100vw, 651px\" \/><\/figure><\/div>\n\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><strong>Table I:<\/strong>&nbsp;Whether you use 5 g or 2 g formats, Resprep EPH fractionation SPE cartridges deliver accurate results consistently, even for the compounds most critical to method requirements as shown below.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Aliphatic Fraction<\/h3>\n\n\n<figure class=\"wp-block-table\">\n<table class=\"has-fixed-layout\">\n<tbody>\n<tr style=\"text-align: center; background-color: #e1effa;\">\n<td>\u00a0<\/td>\n<td>\u00a0<\/td>\n<td colspan=\"2\"><strong>5 g Cartridges<\/strong><\/td>\n<td colspan=\"2\"><strong>2 g Cartridges<\/strong><\/td>\n<\/tr>\n<tr>\n<td><strong>Compound<\/strong><\/td>\n<td><strong>Function<\/strong><\/td>\n<td style=\"text-align: center;\"><strong>Average %<br \/>Recovery<\/strong><\/td>\n<td style=\"text-align: center;\"><strong>%RSD<\/strong><\/td>\n<td style=\"text-align: center;\"><strong>Average %<br \/>Recovery<\/strong><\/td>\n<td style=\"text-align: center;\"><strong>%RSD<\/strong><\/td>\n<\/tr>\n<tr>\n<td>Nonane (C9)<\/td>\n<td>Most-retained aliphatic<\/td>\n<td style=\"text-align: center;\">109.1<\/td>\n<td style=\"text-align: center;\">2.1<\/td>\n<td style=\"text-align: center;\">102.8<\/td>\n<td style=\"text-align: center;\">0.2<\/td>\n<\/tr>\n<tr>\n<td>1- Chlorooctadecane<\/td>\n<td>Surrogate<\/td>\n<td style=\"text-align: center;\">107.0<\/td>\n<td style=\"text-align: center;\">0.6<\/td>\n<td style=\"text-align: center;\">89.8<\/td>\n<td style=\"text-align: center;\">0.4<\/td>\n<\/tr>\n<tr>\n<td>Octacosane (C28)<\/td>\n<td>Aliphatic loading capacity probe<\/td>\n<td style=\"text-align: center;\">109.2<\/td>\n<td style=\"text-align: center;\">1.0<\/td>\n<td style=\"text-align: center;\">101.6<\/td>\n<td style=\"text-align: center;\">0.0<\/td>\n<\/tr>\n<tr>\n<td>Tetracontane (C40)<\/td>\n<td>Least-retained aliphatic<\/td>\n<td style=\"text-align: center;\">106.8<\/td>\n<td style=\"text-align: center;\">0.6<\/td>\n<td style=\"text-align: center;\">100.7<\/td>\n<td style=\"text-align: center;\">0.1<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n\n\n<h3 class=\"wp-block-heading\">Aromatic Fraction<\/h3>\n\n\n<figure class=\"wp-block-table\">\n<table class=\"has-fixed-layout\">\n<tbody>\n<tr style=\"text-align: center; background-color: #e1effa;\">\n<td>\u00a0<\/td>\n<td>\u00a0<\/td>\n<td colspan=\"2\"><strong>5 g Cartridges<\/strong><\/td>\n<td colspan=\"2\"><strong>2 g Cartridges<\/strong><\/td>\n<\/tr>\n<tr>\n<td><strong>Compound<\/strong><\/td>\n<td><strong>Function<\/strong><\/td>\n<td style=\"text-align: center;\"><strong>Average %<br \/>Recovery<\/strong><\/td>\n<td style=\"text-align: center;\"><strong>%RSD<\/strong><\/td>\n<td style=\"text-align: center;\"><strong>Average %<br \/>Recovery<\/strong><\/td>\n<td style=\"text-align: center;\"><strong>%RSD<\/strong><\/td>\n<\/tr>\n<tr>\n<td>1,2,3-Trimethylbenzene<\/td>\n<td>Least-retained aromatic<\/td>\n<td style=\"text-align: center;\">85.3<\/td>\n<td style=\"text-align: center;\">6.7<\/td>\n<td style=\"text-align: center;\">101.9<\/td>\n<td style=\"text-align: center;\">0.5<\/td>\n<\/tr>\n<tr>\n<td>Naphthalene<\/td>\n<td>Breakthrough probe<\/td>\n<td style=\"text-align: center;\">86.6<\/td>\n<td style=\"text-align: center;\">6.8<\/td>\n<td style=\"text-align: center;\">101.5<\/td>\n<td style=\"text-align: center;\">0.5<\/td>\n<\/tr>\n<tr>\n<td>2-Methylnaphthalene<\/td>\n<td>Breakthrough probe<\/td>\n<td style=\"text-align: center;\">86.6<\/td>\n<td style=\"text-align: center;\">6.7<\/td>\n<td style=\"text-align: center;\">101.6<\/td>\n<td style=\"text-align: center;\">0.5<\/td>\n<\/tr>\n<tr>\n<td>2-Fluorobiphenyl<\/td>\n<td>Surrogate<\/td>\n<td style=\"text-align: center;\">86.4<\/td>\n<td style=\"text-align: center;\">6.7<\/td>\n<td style=\"text-align: center;\">101.2<\/td>\n<td style=\"text-align: center;\">0.5<\/td>\n<\/tr>\n<tr>\n<td>2-Bromonaphthalene<\/td>\n<td>Surrogate<\/td>\n<td style=\"text-align: center;\">86.2<\/td>\n<td style=\"text-align: center;\">6.7<\/td>\n<td style=\"text-align: center;\">101.7<\/td>\n<td style=\"text-align: center;\">0.5<\/td>\n<\/tr>\n<tr>\n<td>Phenanthrene<\/td>\n<td>Aromatic loading capacity probe<\/td>\n<td style=\"text-align: center;\">86.2<\/td>\n<td style=\"text-align: center;\">6.7<\/td>\n<td style=\"text-align: center;\">101.4<\/td>\n<td style=\"text-align: center;\">0.6<\/td>\n<\/tr>\n<tr>\n<td><em>o<\/em>-Terphenyl<\/td>\n<td>Surrogate<\/td>\n<td style=\"text-align: center;\">86.2<\/td>\n<td style=\"text-align: center;\">6.7<\/td>\n<td style=\"text-align: center;\">99.7<\/td>\n<td style=\"text-align: center;\">0.4<\/td>\n<\/tr>\n<tr>\n<td>Dibenzo(a,h)anthracene<\/td>\n<td>Most-retained aromatic<\/td>\n<td style=\"text-align: center;\">82.2<\/td>\n<td style=\"text-align: center;\">7.7<\/td>\n<td style=\"text-align: center;\">99.5<\/td>\n<td style=\"text-align: center;\">0.5<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n\n\n<p><em>Fortified concentrations for all fractions were prepared per gram silica and were 800 ppm (C28 and phenanthrene) and 40 ppm (all other compounds) after accounting for the amount of silica in each cartridge format (n = 3). Method requirements: MA EPH and NJ EPH analyte recovery 40%\u2013140%; Texas EPH analyte recovery 60%\u2013140% and surrogate recovery 70%\u2013130%.<\/em><\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Which Cartridge Format do I Need?<\/h2>\n\n\n\n<p><strong>Table II:<\/strong>&nbsp;If your samples don\u2019t require a higher loading capacity, you can save time and reduce solvent consumption by using 2 g cartridges instead of 5 g cartridges.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Solvent Consumption Comparison<\/h3>\n\n\n<figure class=\"wp-block-table\">\n<table class=\"has-fixed-layout\">\n<tbody>\n<tr>\n<td style=\"text-align: center;\" colspan=\"2\" rowspan=\"2\"><strong>Extraction Steps<\/strong><\/td>\n<td style=\"text-align: center;\" colspan=\"2\"><strong>Solvent Consumption (mL)<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\"><strong>5 g Cartridge<\/strong><\/td>\n<td style=\"text-align: center;\"><strong>2 g Cartridge<\/strong><\/td>\n<\/tr>\n<tr>\n<td rowspan=\"2\">Cartridge conditioning<\/td>\n<td>Dichloromethane<\/td>\n<td style=\"text-align: center;\">6<\/td>\n<td style=\"text-align: center;\">3<\/td>\n<\/tr>\n<tr>\n<td>Hexane<\/td>\n<td style=\"text-align: center;\">15<\/td>\n<td style=\"text-align: center;\">5<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"2\">Aliphatic fraction<\/td>\n<td>Sample<\/td>\n<td style=\"text-align: center;\">1<\/td>\n<td style=\"text-align: center;\">1<\/td>\n<\/tr>\n<tr>\n<td>Hexane<\/td>\n<td style=\"text-align: center;\">19<\/td>\n<td style=\"text-align: center;\">5<\/td>\n<\/tr>\n<tr>\n<td>Aromatic fraction<\/td>\n<td>Dichloromethane<\/td>\n<td style=\"text-align: center;\">20<\/td>\n<td style=\"text-align: center;\">6<\/td>\n<\/tr>\n<tr style=\"text-align: center; background-color: #e1effa;\">\n<td style=\"text-align: left;\"><strong>Total solvent used (mL)<\/strong><\/td>\n<td>\u00a0<\/td>\n<td>60<\/td>\n<td>20<\/td>\n<\/tr>\n<tr style=\"text-align: center; background-color: #e1effa;\">\n<td style=\"text-align: left;\"><strong>Total chlorinated solvent used (mL)<\/strong><\/td>\n<td>\u00a0<\/td>\n<td>26<\/td>\n<td>9<\/td>\n<\/tr>\n<tr style=\"text-align: center; background-color: #00b5cb; color: #ffffff;\">\n<td colspan=\"2\">\u00a0<\/td>\n<td colspan=\"2\"><strong>Reduce solvent waste by ~66%!<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n\n\n<h3 class=\"wp-block-heading\">Time-Saving Comparison<\/h3>\n\n\n<figure class=\"wp-block-table\">\n<table class=\"has-fixed-layout\">\n<tbody>\n<tr>\n<td style=\"text-align: center;\" colspan=\"2\" rowspan=\"2\"><strong>Extraction Steps<\/strong><\/td>\n<td style=\"text-align: center;\" colspan=\"2\"><strong>Solvent Consumption (mL)<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\"><strong>5 g Cartridge<\/strong><\/td>\n<td style=\"text-align: center;\"><strong>2 g Cartridge<\/strong><\/td>\n<\/tr>\n<tr>\n<td rowspan=\"2\">Cartridge conditioning<\/td>\n<td>Dichloromethane\u00a0(5-min hold)<\/td>\n<td style=\"text-align: center;\">8<\/td>\n<td style=\"text-align: center;\">6.5<\/td>\n<\/tr>\n<tr>\n<td>Hexane<\/td>\n<td style=\"text-align: center;\">7.5<\/td>\n<td style=\"text-align: center;\">2.5<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"2\">Aliphatic fraction<\/td>\n<td>Sample loading<\/td>\n<td style=\"text-align: center;\">0.5<\/td>\n<td style=\"text-align: center;\">0.5<\/td>\n<\/tr>\n<tr>\n<td>Hexane<\/td>\n<td style=\"text-align: center;\">9<\/td>\n<td style=\"text-align: center;\">2.5<\/td>\n<\/tr>\n<tr>\n<td>Aromatic fraction<\/td>\n<td>Dichloromethane<\/td>\n<td style=\"text-align: center;\">10<\/td>\n<td style=\"text-align: center;\">3<\/td>\n<\/tr>\n<tr>\n<td><strong>Concentration time<\/strong><\/td>\n<td>Two fractions<\/td>\n<td style=\"text-align: center;\">60<\/td>\n<td style=\"text-align: center;\">15<\/td>\n<\/tr>\n<tr>\n<td><strong>Miscellaneous steps<\/strong><\/td>\n<td>Adding solvent, transferring glassware<\/td>\n<td style=\"text-align: center;\">8<\/td>\n<td style=\"text-align: center;\">8<\/td>\n<\/tr>\n<tr style=\"text-align: center; background-color: #e1effa;\">\n<td style=\"text-align: left;\" colspan=\"2\"><strong>Total process time<\/strong><\/td>\n<td>103 min<\/td>\n<td>38 min<\/td>\n<\/tr>\n<tr style=\"text-align: center; background-color: #00b5cb; color: #ffffff;\">\n<td colspan=\"2\">\u00a0<\/td>\n<td colspan=\"2\"><strong>Save over an hour of sample prep time!<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 4:<\/strong>\u00a0Regardless of which cartridge format you use, our moisture-resistant packaging prevents contamination from ambient moisture, ensuring outstanding performance even after storage.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<style>.kb-image43519_707b0d-c4 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43519_707b0d-c4\"><figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"373\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVSS2574B-03-1024x373.jpg\" alt=\"\" class=\"kb-img wp-image-19738\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVSS2574B-03-1024x373.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVSS2574B-03-300x109.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVSS2574B-03-768x280.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVSS2574B-03-1536x560.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVSS2574B-03.jpg 1800w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure><\/div>\n\n<\/div><\/div><\/div>\n\n\n<p><\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-47c7f9e9 wp-block-columns-is-layout-flex\" style=\"background-color:#00b5cb;padding-top:10px;padding-right:20px;padding-bottom:10px;padding-left:20px\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<p class=\"has-theme-palette-9-color has-text-color has-link-color wp-elements-4ab1c2784029b314d6a85f2acb7bb0e2\"><strong><strong>No-risk, no-obligation trial.<\/strong>Free samples of Resprep solid phase extraction (SPE) tubes are available.&nbsp;<a href=\"https:\/\/www.restek.com\/contact-us\" target=\"_blank\" rel=\"noopener\">Contact us<\/a>&nbsp;or your local Restek representative to request yours.<\/strong><\/p>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">Products Mentioned<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/13424\">                                Catalog No. 13424                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Rxi-5ms GC Capillary Column, 30 m, 0.32 mm ID, 0.25 \u00b5m<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/13424\">View Product<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/31479\">                                Catalog No. 31479                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">MA EPH Surrogate Spike Mix, 4000 \u00b5g\/mL, Acetone, 1 mL\/ampul<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/31479\">View Product<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/23310\">                                Catalog No. 23310                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Topaz, Cyclo Double Taper Inlet Liner, 4.0 mm x 6.5 x 78.5, for Agilent GCs, Premium Deactivation, 5-pk.<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/23310\">View Product<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/13423\">                                Catalog No. 13423                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Rxi-5ms GC Capillary Column, 30 m, 0.25 mm ID, 0.25 \u00b5m<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/13423\">View Product<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/23303\">                                Catalog No. 23303                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Topaz, Single Taper Inlet Liner, 4.0 mm x 6.5 x 78.5, for Agilent GCs, w\/Quartz Wool, Premium Deactivation, 5-pk.<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/23303\">View Product<\/a>\n                                                    <\/div>\n                    <\/div>\n                            <\/div>\n        <\/div>\n        \n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>New and improved Resprep EPH fractionation SPE cartridges from Restek completely separate aliphatic and aromatic compounds, and they are guaranteed to produce background levels that are below the strict reporting limits of MA and NJ EPH methods. Superior lot-to-lot reproducibility and storage stability mean every lot will perform the same as the lot before it.<\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[7],"tags":[],"industries-application":[2163,2161,2668,2166],"post-badge":[],"resource-type":[],"product-library":[2372,2384,2375,2402],"resource-technique":[2294,2304],"hf_cat_post":[650],"ppma_author":[414],"class_list":["post-43519","post","type-post","status-publish","format-standard","hentry","category-articles","industries-application-drinking-water-wastewater-testing","industries-application-environmental","industries-application-semivolatiles","industries-application-soil-sediment-biota-testing","product-library-gas-chromatography-products","product-library-gc-columns","product-library-sample-preparation-products","product-library-spe-sle","resource-technique-gas-chromatography-gc","resource-technique-solid-phase-extraction-spe"],"acf":[],"taxonomy_info":{"category":[{"value":7,"label":"Articles"}],"industries-application":[{"value":2163,"label":"Drinking Water &amp; 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