{"id":43713,"date":"2020-12-22T23:00:00","date_gmt":"2020-12-22T23:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/raptor-biphenyl-lc-columns-brochure\/"},"modified":"2026-01-28T16:59:38","modified_gmt":"2026-01-28T16:59:38","slug":"raptor-biphenyl-lc-columns-brochure","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/es\/articles\/gnbr1891\/raptor-biphenyl-lc-columns-brochure","title":{"rendered":"Raptor Biphenyl: Fast, Rugged Raptor Columns with Time-Tested Selectivity"},"content":{"rendered":"\n<p>With Raptor LC columns, Restek chemists became the first to combine the speed of 2.7 and 5 \u03bcm superficially porous particles (also known as SPP or \u201ccore-shell\u201d particles) with the resolution of highly selective USLC technology, improving separations and speeding up analysis times with standard HPLC instruments. Raptor then evolved to bring that same improved speed, efficiency, and selectivity to UHPLC analyses by offering 1.8 \u03bcm particle columns. Learn more about Raptor LC columns at <a href=\"articles\/raptor-lc-columns&quot;\">www.restek.com\/raptor<\/a><\/p>\n\n\n\n<p>Our top priority when developing our SPP line was to create a version of our innovative Biphenyl. The industry-leading Biphenyl is Restek\u2019s most popular LC stationary phase because it is particularly adept at separating compounds that are hard to resolve or that elute early on C18 and other phenyl chemistries. As a result, the rugged Raptor Biphenyl column is extremely useful for fast separations in bioanalytical testing applications, such as drug and metabolite analyses, especially those that require a mass spectrometer (MS). Increasing retention of early-eluting compounds can limit ionization suppression, and the heightened selectivity helps eliminate the need for complex mobile phases that are not well suited for MS detection.<\/p>\n\n\n\n<p>In 2005, Restek was the first to bring you the benefits of the Biphenyl ligand, and we have the experience to maximize the SPP performance of this premier phenyl chemistry for today\u2019s challenging workflows.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<h2 class=\"wp-block-heading\">Column Description:<\/h2>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-905c269e wp-block-columns-is-layout-flex\" style=\"padding-right:20px;padding-left:20px\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><style>.kb-image43713_7f5ea4-82.kb-image-is-ratio-size, .kb-image43713_7f5ea4-82 .kb-image-is-ratio-size{max-width:195px;width:100%;}.wp-block-kadence-column > .kt-inside-inner-col > .kb-image43713_7f5ea4-82.kb-image-is-ratio-size, .wp-block-kadence-column > .kt-inside-inner-col > .kb-image43713_7f5ea4-82 .kb-image-is-ratio-size{align-self:unset;}.kb-image43713_7f5ea4-82 figure{max-width:195px;}.kb-image43713_7f5ea4-82 .image-is-svg, .kb-image43713_7f5ea4-82 .image-is-svg img{width:100%;}.kb-image43713_7f5ea4-82:not(.kb-image-is-ratio-size) .kb-img, .kb-image43713_7f5ea4-82.kb-image-is-ratio-size{padding-top:var(--global-kb-spacing-xs, 1rem);padding-right:var(--global-kb-spacing-xs, 1rem);padding-bottom:var(--global-kb-spacing-xs, 1rem);padding-left:var(--global-kb-spacing-xs, 1rem);}.kb-image43713_7f5ea4-82 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43713_7f5ea4-82\"><figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"392\" height=\"1024\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/phenyl-figure01-392x1024.jpg\" alt=\"\" class=\"kb-img wp-image-66740\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/phenyl-figure01-392x1024.jpg 392w, https:\/\/discover.restek.com\/wp-content\/uploads\/phenyl-figure01-115x300.jpg 115w, https:\/\/discover.restek.com\/wp-content\/uploads\/phenyl-figure01-768x2006.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/phenyl-figure01-588x1536.jpg 588w, https:\/\/discover.restek.com\/wp-content\/uploads\/phenyl-figure01-784x2048.jpg 784w, https:\/\/discover.restek.com\/wp-content\/uploads\/phenyl-figure01-scaled.jpg 980w\" sizes=\"auto, (max-width: 392px) 100vw, 392px\" \/><\/figure><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h4 class=\"wp-block-heading\">Stationary Phase Category:<\/h4>\n\n\n\n<p>Phenyl (L11)<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Ligand Type:<\/h4>\n\n\n\n<p>Biphenyl<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Particle:<\/h4>\n\n\n\n<p>1.8 \u03bcm, 2.7 \u03bcm, or 5 \u03bcm superficially porous particle (SPP or \u201ccore-shell\u201d particle) silica<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Pore Size:<\/h4>\n\n\n\n<p>90 \u00c5<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Surface Area:<\/h4>\n\n\n\n<p>125 m<sup>2<\/sup>\/g (1.8 \u03bcm),<br>130 m<sup>2<\/sup>\/g (2.7 \u03bcm),<br>or 100 m<sup>2<\/sup>\/g (5 \u03bcm)<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h4 class=\"wp-block-heading\">Recommended Usage:<\/h4>\n\n\n\n<p>pH Range: 2.0\u20138.0<br>Maximum Temperature: 80 \u00b0C<br>Maximum Pressure: 1034 bar\/15,000 psi* (1.8 \u03bcm),<br>600 bar\/8700 psi (2.7 \u03bcm); 400 bar\/5800 psi (5 \u03bcm)<br><em>* For maximum lifetime, recommended maximum pressure for 1.8 \u03bcm particles is&nbsp;<\/em><em>830 bar\/12,000 psi.<\/em><\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Properties:<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Increased retention for dipolar, unsaturated, or conjugated solutes.<\/li>\n\n\n\n<li>Enhanced selectivity when used with methanolic mobile phase.<\/li>\n\n\n\n<li>Ideal for increasing sensitivity and selectivity in LC-MS analyses.<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">Switch to a Biphenyl when:<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>You observe limited selectivity on a C18.<\/li>\n\n\n\n<li>You need to increase retention of hydrophilic aromatics.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-905c269e wp-block-columns-is-layout-flex\" style=\"padding-right:20px;padding-left:20px\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"padding-top:var(--wp--preset--spacing--30);padding-right:var(--wp--preset--spacing--30);padding-bottom:var(--wp--preset--spacing--30);padding-left:var(--wp--preset--spacing--30)\">\n<h2 class=\"wp-block-heading\">Column Interaction Profile:<\/h2>\n\n\n<style>.kb-image43713_ecb548-90.kb-image-is-ratio-size, .kb-image43713_ecb548-90 .kb-image-is-ratio-size{max-width:425px;width:100%;}.wp-block-kadence-column > .kt-inside-inner-col > .kb-image43713_ecb548-90.kb-image-is-ratio-size, .wp-block-kadence-column > .kt-inside-inner-col > .kb-image43713_ecb548-90 .kb-image-is-ratio-size{align-self:unset;}.kb-image43713_ecb548-90{max-width:425px;}.image-is-svg.kb-image43713_ecb548-90{-webkit-flex:0 1 100%;flex:0 1 100%;}.image-is-svg.kb-image43713_ecb548-90 img{width:100%;}.kb-image43713_ecb548-90 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<figure class=\"wp-block-kadence-image kb-image43713_ecb548-90 size-medium\"><img loading=\"lazy\" decoding=\"async\" width=\"254\" height=\"300\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/article-profile-gnbr1891c-01-254x300.jpg\" alt=\"\" class=\"kb-img wp-image-12238\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/article-profile-gnbr1891c-01-254x300.jpg 254w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-profile-gnbr1891c-01.jpg 432w\" sizes=\"auto, (max-width: 254px) 100vw, 254px\" \/><\/figure>\n\n\n\n<h4 class=\"wp-block-heading\">Defining Solute Interactions:<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Cation exchange<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">Complementary Solute Interaction:<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Polarizability<\/li>\n\n\n\n<li>Dispersion<\/li>\n<\/ul>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"padding-top:var(--wp--preset--spacing--30);padding-right:var(--wp--preset--spacing--30);padding-bottom:var(--wp--preset--spacing--30);padding-left:var(--wp--preset--spacing--30)\">\n<h2 class=\"wp-block-heading\">Solute Retention Profile:<\/h2>\n\n\n<style>.kb-image43713_31d064-49.kb-image-is-ratio-size, .kb-image43713_31d064-49 .kb-image-is-ratio-size{max-width:407px;width:100%;}.wp-block-kadence-column > .kt-inside-inner-col > .kb-image43713_31d064-49.kb-image-is-ratio-size, .wp-block-kadence-column > .kt-inside-inner-col > .kb-image43713_31d064-49 .kb-image-is-ratio-size{align-self:unset;}.kb-image43713_31d064-49{max-width:407px;}.image-is-svg.kb-image43713_31d064-49{-webkit-flex:0 1 100%;flex:0 1 100%;}.image-is-svg.kb-image43713_31d064-49 img{width:100%;}.kb-image43713_31d064-49 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<figure class=\"wp-block-kadence-image kb-image43713_31d064-49 size-medium_large\"><img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"409\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/article-graph-gnbr2368b-01-1-768x409.jpg\" alt=\"\" class=\"kb-img wp-image-61619\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/article-graph-gnbr2368b-01-1-768x409.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-graph-gnbr2368b-01-1-300x160.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-graph-gnbr2368b-01-1-1024x546.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-graph-gnbr2368b-01-1-1536x818.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-graph-gnbr2368b-01-1.jpg 1800w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/figure>\n\n\n\n<h4 class=\"wp-block-heading\">Target Analyte Structures:<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Aromatic<\/li>\n\n\n\n<li>Dipolar<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">Target Analyte Functionalities:<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Hydrophilic aromatics<\/li>\n\n\n\n<li>Strong dipoles<\/li>\n\n\n\n<li>Lewis acids<\/li>\n\n\n\n<li>Dipolar, unsaturated, or conjugated compounds<\/li>\n\n\n\n<li>Fused-ring compounds with electron withdrawing groups<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">More Aromatic Selectivity than Ordinary Phenyl-Hexyls<\/h2>\n\n\n\n<p>SPP core-shell columns commonly employ traditional phenyl-hexyl stationary phases, but the innovative Biphenyl ligand, developed by Restek\u2019s chemists, is the next generation of phenyl column chemistry. It provides greater aromatic selectivity than commercially available phenyl-hexyl columns [1] and a greater degree of dispersion than conventional phenyls. As a result, the Raptor Biphenyl allows you to more easily separate bioanalytical compounds, such as aromatics (Figures 1 and 2), which elute early or are hard to separate on C18 or other phenyl chemistries.<\/p>\n\n\n\n<p class=\"has-small-font-size\">1. In-house testing based on: M.R. Euerby, P. Petersson, W. Campbell, W. Roe, Chromatographic classification and comparison of commercially available reversed-phase liquid chromatographic columns containing phenyl moieties using principal component analysis, J. Chromatogr. A 1154 (2007) 138\u2013151.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 1:<\/strong>\u00a0Raptor Biphenyl columns exhibit the highest aromatic selectivity compared to other SPP phenyl columns.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"485\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnbr1891c-01-1024x485.jpg\" alt=\"\" class=\"wp-image-12124\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnbr1891c-01-1024x485.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnbr1891c-01-300x142.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnbr1891c-01-768x363.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnbr1891c-01-1536x727.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnbr1891c-01-2048x969.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 2:<\/strong>\u00a0Raptor Biphenyl columns show increased retention for compounds containing electron withdrawing groups. Retention and elution order are dramatically different from a traditional C18.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0546.png\" alt=\"Aromatics on Raptor Biphenyl\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_GN0546<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 50px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 50px\">Conc.<br \/>(\u00b5g\/mL)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Benzene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/71-43-2\/Benzene\" target=\"_blank\" rel=\"noopener\">Benzene<\/a><\/td><td class=\"oth\">1.63<\/td><td class=\"oth\">500<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nitrobenzene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/98-95-3\/Nitrobenzene\" target=\"_blank\" rel=\"noopener\">Nitrobenzene<\/a><\/td><td class=\"oth\">2.09<\/td><td class=\"oth\">100<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 1,3,5-Trinitrobenzene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/99-35-4\/1,3,5-Trinitrobenzene\" target=\"_blank\" rel=\"noopener\">1,3,5-Trinitrobenzene<\/a><\/td><td class=\"oth\">4.83<\/td><td class=\"oth\">100<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2,4-Dinitrotoluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/121-14-2\/2,4-Dinitrotoluene\" target=\"_blank\" rel=\"noopener\">2,4-Dinitrotoluene<\/a><\/td><td class=\"oth\">5.35<\/td><td class=\"oth\">100<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Biphenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309A55?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_GN0546\" rel=\"noopener\">cat.# 9309A55<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 4.6 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Acetonitrile<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>100-500 \u00b5g\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>1 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td>Water:Methanol (50:50)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Flow:<\/th><td>1.2 mL\/min<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>Waters Acquity PDA @ 254 nm<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Waters ACQUITY UPLC H-Class<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0546.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">The New Standard for Performance and Durability for SPP Core-Shell LC Columns<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">Pressure Stability:<\/h3>\n\n\n\n<p>One of the greatest advantages of an SPP column is the ability to achieve fast, efficient separations by operating at higher linear velocities than are possible with a conventional fully porous particle column. However, these higher velocities can also result in higher back pressures. Raptor columns were designed to handle the increased pressures needed to achieve <em>Selectivity Accelerated<\/em> and to handle them far better than other SPP columns on the market (Figure 3).<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 3:<\/strong>\u00a0At high pressures, competitor phenyl-hexyl columns experience a quick and sharp drop-off in efficiency, but Raptor Biphenyl columns are unaffected to at least 3000 injections.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter\"><img decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnbr1891c-03.jpg\" alt=\"\" title=\"-\"><\/figure>\n<\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">Reproducibility:<\/h3>\n\n\n\n<p>To help keep your productivity high and your lab budget low, we know that Raptor Biphenyl columns must produce exceptional selectivity and fast analysis times not just once, but every time. Ruggedness and repeatability are essential, which is why, from the silica and the bonding technique to the packing process and upgraded hardware, every decision that went into creating this column was made to ensure superlative reproducibility from injection to injection (Figure 4) and from lot to lot (Figure 5), regardless of column dimension (Figure 6). We also adopted new quality control (QC) specifications to guarantee the retention time stability you need for worry-free MRM analyses.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 4:<\/strong>\u00a0Even after hundreds of injections, a Raptor Biphenyl column will provide consistent, reliable data.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0565.png\" alt=\"Raptor\u2122 Biphenyl Lifetime Reproducibility\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_CF0565<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cortisol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/50-23-7\/Cortisol\" target=\"_blank\" rel=\"noopener\">Cortisol<\/a><\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 11-Deoxycortisol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/152-58-9\/11-Deoxycortisol\" target=\"_blank\" rel=\"noopener\">11-Deoxycortisol<\/a><\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Estradiol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/50-28-2\/Estradiol\" target=\"_blank\" rel=\"noopener\">Estradiol<\/a><\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Boldenone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/846-48-0\/Boldenone\" target=\"_blank\" rel=\"noopener\">Boldenone<\/a><\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Testosterone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/58-22-0\/Testosterone\" target=\"_blank\" rel=\"noopener\">Testosterone<\/a><\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Androstenedione\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/63-05-8\/Androstenedione\" target=\"_blank\" rel=\"noopener\">Androstenedione<\/a><\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Progesterone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-83-0\/Progesterone\" target=\"_blank\" rel=\"noopener\">Progesterone<\/a><\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor\u2122 Biphenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309A1E?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0565\" rel=\"noopener\">cat.# 9309A1E<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 3.0 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Initial mobile phase<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>50 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.1% Formic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>0.1% Formic acid in acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.700<\/td><td>60<\/td><td>40<\/td><\/tr><tr><td>3.00<\/td><td>0.700<\/td><td>20<\/td><td>80<\/td><\/tr><tr><td>3.01<\/td><td>0.700<\/td><td>60<\/td><td>40<\/td><\/tr><tr><td>5.00<\/td><td>0.700<\/td><td>60<\/td><td>40<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>Waters Xevo TQ-S<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Waters<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0565.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 5:<\/strong>\u00a0From one lot to the next, every Raptor Biphenyl column you purchase will perform the same.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0562.png\" alt=\"Raptor\u2122 Biphenyl Lot-to-Lot Reproducibility\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_CF0562<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cortisol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/50-23-7\/Cortisol\" target=\"_blank\" rel=\"noopener\">Cortisol<\/a><\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 11-Deoxycortisol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/152-58-9\/11-Deoxycortisol\" target=\"_blank\" rel=\"noopener\">11-Deoxycortisol<\/a><\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Estradiol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/50-28-2\/Estradiol\" target=\"_blank\" rel=\"noopener\">Estradiol<\/a><\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Boldenone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/846-48-0\/Boldenone\" target=\"_blank\" rel=\"noopener\">Boldenone<\/a><\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Testosterone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/58-22-0\/Testosterone\" target=\"_blank\" rel=\"noopener\">Testosterone<\/a><\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Androstenedione\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/63-05-8\/Androstenedione\" target=\"_blank\" rel=\"noopener\">Androstenedione<\/a><\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Progesterone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-83-0\/Progesterone\" target=\"_blank\" rel=\"noopener\">Progesterone<\/a><\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor\u2122 Biphenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309A1E?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0562\" rel=\"noopener\">cat.# 9309A1E<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 3.0 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Initial mobile phase<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>50 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.1% Formic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>0.1% Formic acid in acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.700<\/td><td>60<\/td><td>40<\/td><\/tr><tr><td>3.00<\/td><td>0.700<\/td><td>20<\/td><td>80<\/td><\/tr><tr><td>3.01<\/td><td>0.700<\/td><td>60<\/td><td>40<\/td><\/tr><tr><td>5.00<\/td><td>0.700<\/td><td>60<\/td><td>40<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>Waters Xevo TQ-S<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Waters<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0562.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 6:<\/strong>\u00a0Regardless of the dimension, Raptor columns are rugged enough to last well past 1000 injections, even in the high-pressure conditions of UHPLC.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"475\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnbr1891c-06-1024x475.jpg\" alt=\"\" class=\"wp-image-12136\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnbr1891c-06-1024x475.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnbr1891c-06-300x139.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnbr1891c-06-768x357.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnbr1891c-06-1536x713.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/article-figure-gnbr1891c-06-2048x951.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Clinically Proven to Optimize Your Bioanalytical Workflows<\/h2>\n\n\n\n<p>For over a decade, the Restek Biphenyl has been the column of choice for clinical testing because of its ability to provide highly retentive, selective, and rugged reversed-phase separations of drugs and metabolites. By bringing the speed of SPP to the Biphenyl family, the Raptor Biphenyl provides clinical labs with an even faster option for a wide variety of clinical assays.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Rugged Pain Panels from Urine in Under 3.5 Minutes<\/h3>\n\n\n\n<p>Pain panels can be difficult to optimize and reproduce due to the limited selectivity of C18 and phenyl-hexyl phases but not on the Raptor Biphenyl. Complete your pain panel analysis with a five-minute cycle time and complete isobaric resolution using Raptor Biphenyl columns (Figure 7). Popular competitor columns offer tailing peaks, longer run times, and coelutions; the Raptor Biphenyl exhibits the selectivity and performance needed for this critical analysis.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 7:<\/strong> Raptor Biphenyl columns offer pain panel analyses with complete isobaric resolution in under five minutes!<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<h3 class=\"wp-block-heading has-text-color has-link-color wp-elements-697ba569a807a8791641c52111bc1dd7\" style=\"color:#68bd45\">Raptor Biphenyl<\/h3>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0568.png\" alt=\"Pain Panel in Urine on Raptor Biphenyl (50 x 3.0 mm) by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_CF0568<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor ion<\/th><th style=\"text-align: center;width: 75px\">Product ion 1<\/th><th style=\"text-align: center;width: 75px\">Product ion 2<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Morphine*\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-27-2\/Morphine\" target=\"_blank\" rel=\"noopener\">Morphine*<\/a><\/td><td class=\"oth\">1.34<\/td><td class=\"oth\">286.2<\/td><td class=\"oth\">152.3<\/td><td class=\"oth\">165.3<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Oxymorphone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/76-41-5\/Oxymorphone\" target=\"_blank\" rel=\"noopener\">Oxymorphone<\/a><\/td><td class=\"oth\">1.40<\/td><td class=\"oth\">302.1<\/td><td class=\"oth\">227.3<\/td><td class=\"oth\">198.2<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Hydromorphone*\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/466-99-9\/Hydromorphone\" target=\"_blank\" rel=\"noopener\">Hydromorphone*<\/a><\/td><td class=\"oth\">1.52<\/td><td class=\"oth\">286.1<\/td><td class=\"oth\">185.3<\/td><td class=\"oth\">128.2<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Amphetamine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/300-62-9\/Amphetamine\" target=\"_blank\" rel=\"noopener\">Amphetamine<\/a><\/td><td class=\"oth\">1.62<\/td><td class=\"oth\">136.0<\/td><td class=\"oth\">91.3<\/td><td class=\"oth\">119.2<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Methamphetamine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/537-46-2\/Methamphetamine\" target=\"_blank\" rel=\"noopener\">Methamphetamine<\/a><\/td><td class=\"oth\">1.84<\/td><td class=\"oth\">150.0<\/td><td class=\"oth\">91.2<\/td><td class=\"oth\">119.3<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Codeine*\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/76-57-3\/Codeine\" target=\"_blank\" rel=\"noopener\">Codeine*<\/a><\/td><td class=\"oth\">1.91<\/td><td class=\"oth\">300.2<\/td><td class=\"oth\">165.4<\/td><td class=\"oth\">153.2<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Oxycodone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/76-42-6\/Oxycodone\" target=\"_blank\" rel=\"noopener\">Oxycodone<\/a><\/td><td class=\"oth\">2.02<\/td><td class=\"oth\">316.1<\/td><td class=\"oth\">241.3<\/td><td class=\"oth\">256.4<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Hydrocodone*\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/125-29-1\/Hydrocodone\" target=\"_blank\" rel=\"noopener\">Hydrocodone*<\/a><\/td><td class=\"oth\">2.06<\/td><td class=\"oth\">300.1<\/td><td class=\"oth\">199.3<\/td><td class=\"oth\">128.3<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Norbuprenorphine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/78715-23-8\/Norbuprenorphine\" target=\"_blank\" rel=\"noopener\">Norbuprenorphine<\/a><\/td><td class=\"oth\">2.59<\/td><td class=\"oth\">414.1<\/td><td class=\"oth\">83.4<\/td><td class=\"oth\">101.0<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor ion<\/th><th style=\"text-align: center;width: 75px\">Product ion 1<\/th><th style=\"text-align: center;width: 75px\">Product ion 2<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Meprobamate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-53-4\/Meprobamate\" target=\"_blank\" rel=\"noopener\">Meprobamate<\/a><\/td><td class=\"oth\">2.61<\/td><td class=\"oth\">219.0<\/td><td class=\"oth\">158.4<\/td><td class=\"oth\">97.2<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Fentanyl\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/437-38-7\/Fentanyl\" target=\"_blank\" rel=\"noopener\">Fentanyl<\/a><\/td><td class=\"oth\">2.70<\/td><td class=\"oth\">337.2<\/td><td class=\"oth\">188.4<\/td><td class=\"oth\">105.2<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Buprenorphine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/52485-79-7\/Buprenorphine\" target=\"_blank\" rel=\"noopener\">Buprenorphine<\/a><\/td><td class=\"oth\">2.70<\/td><td class=\"oth\">468.3<\/td><td class=\"oth\">396.4<\/td><td class=\"oth\">414.5<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Flurazepam\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/17617-23-1\/Flurazepam\" target=\"_blank\" rel=\"noopener\">Flurazepam<\/a><\/td><td class=\"oth\">2.73<\/td><td class=\"oth\">388.2<\/td><td class=\"oth\">315.2<\/td><td class=\"oth\">288.3<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sufentanil\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56030-54-7\/Sufentanil\" target=\"_blank\" rel=\"noopener\">Sufentanil<\/a><\/td><td class=\"oth\">2.77<\/td><td class=\"oth\">387.2<\/td><td class=\"oth\">238.5<\/td><td class=\"oth\">111.3<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Methadone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/76-99-3\/Methadone\" target=\"_blank\" rel=\"noopener\">Methadone<\/a><\/td><td class=\"oth\">2.86<\/td><td class=\"oth\">310.2<\/td><td class=\"oth\">265.3<\/td><td class=\"oth\">105.3<\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Carisoprodol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/78-44-4\/Carisoprodol\" target=\"_blank\" rel=\"noopener\">Carisoprodol<\/a><\/td><td class=\"oth\">2.87<\/td><td class=\"oth\">261.2<\/td><td class=\"oth\">176.3<\/td><td class=\"oth\">158.1<\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Lorazepam\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/846-49-1\/Lorazepam\" target=\"_blank\" rel=\"noopener\">Lorazepam<\/a><\/td><td class=\"oth\">3.03<\/td><td class=\"oth\">321.0<\/td><td class=\"oth\">275.4<\/td><td class=\"oth\">303.1<\/td><\/tr>\n<tr><td class=\"num\">18.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Diazepam\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/439-14-5\/Diazepam\" target=\"_blank\" rel=\"noopener\">Diazepam<\/a><\/td><td class=\"oth\">3.31<\/td><td class=\"oth\">285.1<\/td><td class=\"oth\">193.2<\/td><td class=\"oth\">153.9<\/td><\/tr>\n<\/tbody><\/table><div class=\"peaknotes\">*An extracted ion chromatogram (XIC) of the isobars is presented in the inset.<\/div><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Biphenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309A5E?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0568\" rel=\"noopener\">cat.# 9309A5E<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 3.0 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Urine:mobile phase A:mobile phase B (17:76:7)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>10-100 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water + 0.1% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol + 0.1% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.6<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>1.50<\/td><td>0.6<\/td><td>55<\/td><td>45<\/td><\/tr><tr><td>2.50<\/td><td>0.6<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>3.70<\/td><td>0.6<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>3.71<\/td><td>0.6<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>5.00<\/td><td>0.6<\/td><td>90<\/td><td>10<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>AB SCIEX API 4000 MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Source:<\/th><td>TurboIonSpray<sup>&reg;<\/sup> <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>API LC-MS\/MS<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Lorazepam was prepared at 100 ng\/mL; all other analytes are 10 ng\/mL.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0568.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<h3 class=\"wp-block-heading has-text-color has-link-color wp-elements-0d22b0ffbc7f3a19ef1ac1e1165b4855\" style=\"color:#68bd45\">Competitor B SPP Phenyl-Hexyl<\/h3>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0573.png\" alt=\"Pain Panel in Urine on Competitor SPP Phenyl-Hexyl (50 x 4.6 mm) by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_CF0573<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor ion<\/th><th style=\"text-align: center;width: 75px\">Product ion 1<\/th><th style=\"text-align: center;width: 75px\">Product ion 2<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Oxymorphone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/76-41-5\/Oxymorphone\" target=\"_blank\" rel=\"noopener\">Oxymorphone<\/a><\/td><td class=\"oth\">2.46<\/td><td class=\"oth\">302.1<\/td><td class=\"oth\">227.3<\/td><td class=\"oth\">198.2<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Morphine*\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-27-2\/Morphine\" target=\"_blank\" rel=\"noopener\">Morphine*<\/a><\/td><td class=\"oth\">2.48<\/td><td class=\"oth\">286.2<\/td><td class=\"oth\">152.3<\/td><td class=\"oth\">165.3<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Hydromorphone*\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/466-99-9\/Hydromorphone\" target=\"_blank\" rel=\"noopener\">Hydromorphone*<\/a><\/td><td class=\"oth\">2.66<\/td><td class=\"oth\">286.1<\/td><td class=\"oth\">185.3<\/td><td class=\"oth\">128.2<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Amphetamine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/300-62-9\/Amphetamine\" target=\"_blank\" rel=\"noopener\">Amphetamine<\/a><\/td><td class=\"oth\">2.92<\/td><td class=\"oth\">136.0<\/td><td class=\"oth\">91.3<\/td><td class=\"oth\">119.2<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Codeine*\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/76-57-3\/Codeine\" target=\"_blank\" rel=\"noopener\">Codeine*<\/a><\/td><td class=\"oth\">2.97<\/td><td class=\"oth\">300.2<\/td><td class=\"oth\">165.4<\/td><td class=\"oth\">153.2<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Oxycodone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/76-42-6\/Oxycodone\" target=\"_blank\" rel=\"noopener\">Oxycodone<\/a><\/td><td class=\"oth\">3.01<\/td><td class=\"oth\">316.1<\/td><td class=\"oth\">241.3<\/td><td class=\"oth\">256.4<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Methamphetamine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/537-46-2\/Methamphetamine\" target=\"_blank\" rel=\"noopener\">Methamphetamine<\/a><\/td><td class=\"oth\">3.03<\/td><td class=\"oth\">150.0<\/td><td class=\"oth\">91.2<\/td><td class=\"oth\">119.3<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Hydrocodone*\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/125-29-1\/Hydrocodone\" target=\"_blank\" rel=\"noopener\">Hydrocodone*<\/a><\/td><td class=\"oth\">3.16<\/td><td class=\"oth\">300.1<\/td><td class=\"oth\">199.3<\/td><td class=\"oth\">128.3<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Meprobamate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-53-4\/Meprobamate\" target=\"_blank\" rel=\"noopener\">Meprobamate<\/a><\/td><td class=\"oth\">3.65<\/td><td class=\"oth\">219.0<\/td><td class=\"oth\">158.4<\/td><td class=\"oth\">97.2<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor ion<\/th><th style=\"text-align: center;width: 75px\">Product ion 1<\/th><th style=\"text-align: center;width: 75px\">Product ion 2<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Norbuprenorphine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/78715-23-8\/Norbuprenorphine\" target=\"_blank\" rel=\"noopener\">Norbuprenorphine<\/a><\/td><td class=\"oth\">3.92<\/td><td class=\"oth\">414.1<\/td><td class=\"oth\">83.4<\/td><td class=\"oth\">101.0<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Carisoprodol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/78-44-4\/Carisoprodol\" target=\"_blank\" rel=\"noopener\">Carisoprodol<\/a><\/td><td class=\"oth\">4.18<\/td><td class=\"oth\">261.2<\/td><td class=\"oth\">176.3<\/td><td class=\"oth\">158.1<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Fentanyl\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/437-38-7\/Fentanyl\" target=\"_blank\" rel=\"noopener\">Fentanyl<\/a><\/td><td class=\"oth\">4.26<\/td><td class=\"oth\">337.2<\/td><td class=\"oth\">188.4<\/td><td class=\"oth\">105.2<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Flurazepam\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/17617-23-1\/Flurazepam\" target=\"_blank\" rel=\"noopener\">Flurazepam<\/a><\/td><td class=\"oth\">4.30<\/td><td class=\"oth\">388.2<\/td><td class=\"oth\">315.2<\/td><td class=\"oth\">288.3<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Lorazepam\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/846-49-1\/Lorazepam\" target=\"_blank\" rel=\"noopener\">Lorazepam<\/a><\/td><td class=\"oth\">4.40<\/td><td class=\"oth\">321.0<\/td><td class=\"oth\">275.4<\/td><td class=\"oth\">303.1<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Methadone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/76-99-3\/Methadone\" target=\"_blank\" rel=\"noopener\">Methadone<\/a><\/td><td class=\"oth\">4.44<\/td><td class=\"oth\">310.2<\/td><td class=\"oth\">265.3<\/td><td class=\"oth\">105.3<\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sufentanil\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56030-54-7\/Sufentanil\" target=\"_blank\" rel=\"noopener\">Sufentanil<\/a><\/td><td class=\"oth\">4.65<\/td><td class=\"oth\">387.2<\/td><td class=\"oth\">238.5<\/td><td class=\"oth\">111.3<\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Diazepam\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/439-14-5\/Diazepam\" target=\"_blank\" rel=\"noopener\">Diazepam<\/a><\/td><td class=\"oth\">4.80<\/td><td class=\"oth\">285.1<\/td><td class=\"oth\">193.2<\/td><td class=\"oth\">153.9<\/td><\/tr>\n<tr><td class=\"num\">18.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Buprenorphine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/52485-79-7\/Buprenorphine\" target=\"_blank\" rel=\"noopener\">Buprenorphine<\/a><\/td><td class=\"oth\">4.86<\/td><td class=\"oth\">468.3<\/td><td class=\"oth\">396.4<\/td><td class=\"oth\">414.5<\/td><\/tr>\n<\/tbody><\/table><div class=\"peaknotes\">*An extracted ion chromatogram (XIC) of the isobars is presented in the inset.<\/div><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Competitor SPP Phenyl-Hexyl<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 4.6 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.6 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>100 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>22 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Urine:mobile phase A:mobile phase B (17:76:7)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>10-100 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water + 10 mM ammonium formate <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol + 0.1% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.6<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>4.00<\/td><td>0.6<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>5.00<\/td><td>0.6<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>5.10<\/td><td>0.6<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>7.00<\/td><td>0.6<\/td><td>95<\/td><td>5<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>AB SCIEX API 4000\u2122 MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Source:<\/th><td>TurboIonSpray<sup>&reg;<\/sup> <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>API LC-MS\/MS<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Lorazepam was prepared at 100 ng\/mL; all other analytes are 10 ng\/mL. Column and conditions used were specifically recommended or published by the manufacturer for this assay.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0573.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<h3 class=\"wp-block-heading has-text-color has-link-color wp-elements-fd8f474b9dc77b0d2993b7ac15c30b7c\" style=\"color:#68bd45\">Competitor B SPP C18<\/h3>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0577.png\" alt=\"Pain Panel in Urine on Competitor SPP C18 (50 x 3.0 mm) by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_CF0577<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor ion<\/th><th style=\"text-align: center;width: 75px\">Product ion 1<\/th><th style=\"text-align: center;width: 75px\">Product ion 2<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Oxymorphone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/76-41-5\/Oxymorphone\" target=\"_blank\" rel=\"noopener\">Oxymorphone<\/a><\/td><td class=\"oth\">1.47<\/td><td class=\"oth\">302.1<\/td><td class=\"oth\">227.3<\/td><td class=\"oth\">198.2<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Morphine*\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-27-2\/Morphine\" target=\"_blank\" rel=\"noopener\">Morphine*<\/a><\/td><td class=\"oth\">1.56<\/td><td class=\"oth\">286.2<\/td><td class=\"oth\">152.3<\/td><td class=\"oth\">165.3<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Hydromorphone*\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/466-99-9\/Hydromorphone\" target=\"_blank\" rel=\"noopener\">Hydromorphone*<\/a><\/td><td class=\"oth\">1.67<\/td><td class=\"oth\">286.1<\/td><td class=\"oth\">185.3<\/td><td class=\"oth\">128.2<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Oxycodone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/76-42-6\/Oxycodone\" target=\"_blank\" rel=\"noopener\">Oxycodone<\/a><\/td><td class=\"oth\">1.97<\/td><td class=\"oth\">316.1<\/td><td class=\"oth\">241.3<\/td><td class=\"oth\">256.4<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Codeine*\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/76-57-3\/Codeine\" target=\"_blank\" rel=\"noopener\">Codeine*<\/a><\/td><td class=\"oth\">1.97<\/td><td class=\"oth\">300.2<\/td><td class=\"oth\">165.4<\/td><td class=\"oth\">153.2<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Amphetamine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/300-62-9\/Amphetamine\" target=\"_blank\" rel=\"noopener\">Amphetamine<\/a><\/td><td class=\"oth\">2.00<\/td><td class=\"oth\">136.0<\/td><td class=\"oth\">91.3<\/td><td class=\"oth\">119.2<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Methamphetamine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/537-46-2\/Methamphetamine\" target=\"_blank\" rel=\"noopener\">Methamphetamine<\/a><\/td><td class=\"oth\">2.02<\/td><td class=\"oth\">150.0<\/td><td class=\"oth\">91.2<\/td><td class=\"oth\">119.3<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Hydrocodone*\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/125-29-1\/Hydrocodone\" target=\"_blank\" rel=\"noopener\">Hydrocodone*<\/a><\/td><td class=\"oth\">2.02<\/td><td class=\"oth\">300.1<\/td><td class=\"oth\">199.3<\/td><td class=\"oth\">128.3<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Meprobamate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-53-4\/Meprobamate\" target=\"_blank\" rel=\"noopener\">Meprobamate<\/a><\/td><td class=\"oth\">2.61<\/td><td class=\"oth\">219.0<\/td><td class=\"oth\">158.4<\/td><td class=\"oth\">97.2<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor ion<\/th><th style=\"text-align: center;width: 75px\">Product ion 1<\/th><th style=\"text-align: center;width: 75px\">Product ion 2<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Norbuprenorphine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/78715-23-8\/Norbuprenorphine\" target=\"_blank\" rel=\"noopener\">Norbuprenorphine<\/a><\/td><td class=\"oth\">2.63<\/td><td class=\"oth\">414.1<\/td><td class=\"oth\">83.4<\/td><td class=\"oth\">101.0<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Fentanyl\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/437-38-7\/Fentanyl\" target=\"_blank\" rel=\"noopener\">Fentanyl<\/a><\/td><td class=\"oth\">2.85<\/td><td class=\"oth\">337.2<\/td><td class=\"oth\">188.4<\/td><td class=\"oth\">105.2<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Flurazepam\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/17617-23-1\/Flurazepam\" target=\"_blank\" rel=\"noopener\">Flurazepam<\/a><\/td><td class=\"oth\">2.87<\/td><td class=\"oth\">388.2<\/td><td class=\"oth\">315.2<\/td><td class=\"oth\">288.3<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Methadone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/76-99-3\/Methadone\" target=\"_blank\" rel=\"noopener\">Methadone<\/a><\/td><td class=\"oth\">3.00<\/td><td class=\"oth\">310.2<\/td><td class=\"oth\">265.3<\/td><td class=\"oth\">105.3<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Carisoprodol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/78-44-4\/Carisoprodol\" target=\"_blank\" rel=\"noopener\">Carisoprodol<\/a><\/td><td class=\"oth\">3.03<\/td><td class=\"oth\">261.2<\/td><td class=\"oth\">176.3<\/td><td class=\"oth\">158.1<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Lorazepam\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/846-49-1\/Lorazepam\" target=\"_blank\" rel=\"noopener\">Lorazepam<\/a><\/td><td class=\"oth\">3.06<\/td><td class=\"oth\">321.0<\/td><td class=\"oth\">275.4<\/td><td class=\"oth\">303.1<\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sufentanil\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56030-54-7\/Sufentanil\" target=\"_blank\" rel=\"noopener\">Sufentanil<\/a><\/td><td class=\"oth\">3.19<\/td><td class=\"oth\">387.2<\/td><td class=\"oth\">238.5<\/td><td class=\"oth\">111.3<\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Diazepam\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/439-14-5\/Diazepam\" target=\"_blank\" rel=\"noopener\">Diazepam<\/a><\/td><td class=\"oth\">3.27<\/td><td class=\"oth\">285.1<\/td><td class=\"oth\">193.2<\/td><td class=\"oth\">153.9<\/td><\/tr>\n<tr><td class=\"num\">18.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Buprenorphine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/52485-79-7\/Buprenorphine\" target=\"_blank\" rel=\"noopener\">Buprenorphine<\/a><\/td><td class=\"oth\">3.56<\/td><td class=\"oth\">468.3<\/td><td class=\"oth\">396.4<\/td><td class=\"oth\">414.5<\/td><\/tr>\n<\/tbody><\/table><div class=\"peaknotes\">*An extracted ion chromatogram (XIC) of the isobars is presented in the inset.<\/div><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Competitor SPP C18<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 3.0 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.6 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>100 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>25 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Urine:mobile phase A:mobile phase B (17:76:7)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>10-100 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water + 10 mM ammonium formate <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol + 0.1% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>3.00<\/td><td>0.5<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>4.00<\/td><td>0.5<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>4.10<\/td><td>0.5<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>5.00<\/td><td>0.5<\/td><td>95<\/td><td>5<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>AB SCIEX API 4000\u2122 MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Source:<\/th><td>TurboIonSpray<sup>&reg;<\/sup> <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>API LC-MS\/MS<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Lorazepam was prepared at 100 ng\/mL; all other analytes are 10 ng\/mL. Column and conditions used were specifically recommended or published by the manufacturer for this assay.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0577.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">Bisphenols Resolved Quickly with Biphenyl Selectivity, Not Mobile Phase Adjustments<\/h3>\n\n\n\n<p>With health and safety analyses\u2014like the evaluation of bisphenols in consumer products\u2014speed, reliability, and simplicity are crucial. The Raptor Biphenyl column is able to resolve challenging bioanalytical compound sets quickly (e.g., 15 bisphenols in eight minutes shown below [Figures 8 &amp; 9]) using a simple gradient of mobile phases that require NO additives. When you need baseline resolution for UV detection or high-throughput mass spectrometer applications, especially when fast, trusted, and easy solutions are so vital, the rugged and reproducible Raptor Biphenyl column is the ideal choice.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 8:<\/strong>\u00a0Separate bisphenols fast with NO mobile phase additives.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0518.png\" alt=\"Bisphenols on Raptor Biphenyl 1.8 \u03bcm\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0518<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol S\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/80-09-1\/Bisphenol S\" target=\"_blank\" rel=\"noopener\">Bisphenol S<\/a><\/td><td class=\"oth\">0.84<\/td><td class=\"oth\">5.00<\/td><td class=\"oth\">249.2<\/td><td class=\"oth\">108.1<\/td><td class=\"oth\">92.1<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol F\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2467-02-9\/Bisphenol F\" target=\"_blank\" rel=\"noopener\">Bisphenol F<\/a><\/td><td class=\"oth\">1.62<\/td><td class=\"oth\">350<\/td><td class=\"oth\">199.3<\/td><td class=\"oth\">93.1<\/td><td class=\"oth\">105.1<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol E\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2081-08-5\/Bisphenol E\" target=\"_blank\" rel=\"noopener\">Bisphenol E<\/a><\/td><td class=\"oth\">2.06<\/td><td class=\"oth\">100<\/td><td class=\"oth\">213.3<\/td><td class=\"oth\">198.3<\/td><td class=\"oth\">197.4<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol A\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/80-05-7\/Bisphenol A\" target=\"_blank\" rel=\"noopener\">Bisphenol A<\/a><\/td><td class=\"oth\">2.50<\/td><td class=\"oth\">100<\/td><td class=\"oth\">227.3<\/td><td class=\"oth\">212.3<\/td><td class=\"oth\">133.1<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol AF\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1478-61-1\/Bisphenol AF\" target=\"_blank\" rel=\"noopener\">Bisphenol AF<\/a><\/td><td class=\"oth\">2.71<\/td><td class=\"oth\">2.00<\/td><td class=\"oth\">335.2<\/td><td class=\"oth\">265.3<\/td><td class=\"oth\">177.3<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol B\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/77-40-7\/Bisphenol B\" target=\"_blank\" rel=\"noopener\">Bisphenol B<\/a><\/td><td class=\"oth\">3.13<\/td><td class=\"oth\">100<\/td><td class=\"oth\">241.3<\/td><td class=\"oth\">212.4<\/td><td class=\"oth\">211.3<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol C\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/79-97-0\/Bisphenol C\" target=\"_blank\" rel=\"noopener\">Bisphenol C<\/a><\/td><td class=\"oth\">3.43<\/td><td class=\"oth\">350<\/td><td class=\"oth\">255.3<\/td><td class=\"oth\">240.4<\/td><td class=\"oth\">147.3<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol AP\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1571-75-1\/Bisphenol AP\" target=\"_blank\" rel=\"noopener\">Bisphenol AP<\/a><\/td><td class=\"oth\">3.98<\/td><td class=\"oth\">25.0<\/td><td class=\"oth\">289.3<\/td><td class=\"oth\">274.3<\/td><td class=\"oth\">273.3<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol Z\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/843-55-0\/Bisphenol Z\" target=\"_blank\" rel=\"noopener\">Bisphenol Z<\/a><\/td><td class=\"oth\">4.25<\/td><td class=\"oth\">250<\/td><td class=\"oth\">267.2<\/td><td class=\"oth\">173.4<\/td><td class=\"oth\">145.2<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol G\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/127-54-8\/Bisphenol G\" target=\"_blank\" rel=\"noopener\">Bisphenol G<\/a><\/td><td class=\"oth\">4.72<\/td><td class=\"oth\">250<\/td><td class=\"oth\">311.2<\/td><td class=\"oth\">295.4<\/td><td class=\"oth\">296.4<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol FL\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/3236-71-3\/Bisphenol FL\" target=\"_blank\" rel=\"noopener\">Bisphenol FL<\/a><\/td><td class=\"oth\">4.90<\/td><td class=\"oth\">50.0<\/td><td class=\"oth\">348.8<\/td><td class=\"oth\">256.2<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol BP\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1844-01-5\/Bisphenol BP\" target=\"_blank\" rel=\"noopener\">Bisphenol BP<\/a><\/td><td class=\"oth\">5.14<\/td><td class=\"oth\">50.0<\/td><td class=\"oth\">351.2<\/td><td class=\"oth\">273.3<\/td><td class=\"oth\">274.3<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol M\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/13595-25-0\/Bisphenol M\" target=\"_blank\" rel=\"noopener\">Bisphenol M<\/a><\/td><td class=\"oth\">5.39<\/td><td class=\"oth\">15.0<\/td><td class=\"oth\">345.2<\/td><td class=\"oth\">330.3<\/td><td class=\"oth\">251.4<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol P\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2167-51-3\/Bisphenol P\" target=\"_blank\" rel=\"noopener\">Bisphenol P<\/a><\/td><td class=\"oth\">5.67<\/td><td class=\"oth\">50.0<\/td><td class=\"oth\">345.2<\/td><td class=\"oth\">330.4<\/td><td class=\"oth\">315.3<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol PH\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/24038-68-4\/Bisphenol PH\" target=\"_blank\" rel=\"noopener\">Bisphenol PH<\/a><\/td><td class=\"oth\">6.11<\/td><td class=\"oth\">350<\/td><td class=\"oth\">379.2<\/td><td class=\"oth\">209.4<\/td><td class=\"oth\">364.4<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Biphenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0518\" rel=\"noopener\">cat.# 9309252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>1.8 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>25 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>75:25 Water:methanol<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>2.00-350 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.45<\/td><td>50<\/td><td>50<\/td><\/tr><tr><td>6.50<\/td><td>0.45<\/td><td>10<\/td><td>90<\/td><\/tr><tr><td>6.51<\/td><td>0.45<\/td><td>50<\/td><td>50<\/td><\/tr><tr><td>8.00<\/td><td>0.45<\/td><td>50<\/td><td>50<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0518.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<p><strong>Figure 9:<\/strong>&nbsp;Lot-to-lot reproducibility means the same performance column after column for bisphenols and other health and safety analyses.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0519.png\" alt=\"Inter-Lot Comparison: Bisphenols on Raptor Biphenyl 1.8 \u03bcm\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0519<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol S\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/80-09-1\/Bisphenol S\" target=\"_blank\" rel=\"noopener\">Bisphenol S<\/a><\/td><td class=\"oth\">0.84<\/td><td class=\"oth\">5.00<\/td><td class=\"oth\">249.2<\/td><td class=\"oth\">108.1<\/td><td class=\"oth\">92.1<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol F\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2467-02-9\/Bisphenol F\" target=\"_blank\" rel=\"noopener\">Bisphenol F<\/a><\/td><td class=\"oth\">1.62<\/td><td class=\"oth\">350<\/td><td class=\"oth\">199.3<\/td><td class=\"oth\">93.1<\/td><td class=\"oth\">105.1<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol E\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2081-08-5\/Bisphenol E\" target=\"_blank\" rel=\"noopener\">Bisphenol E<\/a><\/td><td class=\"oth\">2.06<\/td><td class=\"oth\">100<\/td><td class=\"oth\">213.3<\/td><td class=\"oth\">198.3<\/td><td class=\"oth\">197.4<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol A\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/80-05-7\/Bisphenol A\" target=\"_blank\" rel=\"noopener\">Bisphenol A<\/a><\/td><td class=\"oth\">2.50<\/td><td class=\"oth\">100<\/td><td class=\"oth\">227.3<\/td><td class=\"oth\">212.3<\/td><td class=\"oth\">133.1<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol AF\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1478-61-1\/Bisphenol AF\" target=\"_blank\" rel=\"noopener\">Bisphenol AF<\/a><\/td><td class=\"oth\">2.71<\/td><td class=\"oth\">2.00<\/td><td class=\"oth\">335.2<\/td><td class=\"oth\">265.3<\/td><td class=\"oth\">177.3<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol B\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/77-40-7\/Bisphenol B\" target=\"_blank\" rel=\"noopener\">Bisphenol B<\/a><\/td><td class=\"oth\">3.13<\/td><td class=\"oth\">100<\/td><td class=\"oth\">241.3<\/td><td class=\"oth\">212.4<\/td><td class=\"oth\">211.3<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol C\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/79-97-0\/Bisphenol C\" target=\"_blank\" rel=\"noopener\">Bisphenol C<\/a><\/td><td class=\"oth\">3.43<\/td><td class=\"oth\">350<\/td><td class=\"oth\">255.3<\/td><td class=\"oth\">240.4<\/td><td class=\"oth\">147.3<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol AP\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1571-75-1\/Bisphenol AP\" target=\"_blank\" rel=\"noopener\">Bisphenol AP<\/a><\/td><td class=\"oth\">3.98<\/td><td class=\"oth\">25.0<\/td><td class=\"oth\">289.3<\/td><td class=\"oth\">274.3<\/td><td class=\"oth\">273.3<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol Z\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/843-55-0\/Bisphenol Z\" target=\"_blank\" rel=\"noopener\">Bisphenol Z<\/a><\/td><td class=\"oth\">4.25<\/td><td class=\"oth\">250<\/td><td class=\"oth\">267.2<\/td><td class=\"oth\">173.4<\/td><td class=\"oth\">145.2<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol G\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/127-54-8\/Bisphenol G\" target=\"_blank\" rel=\"noopener\">Bisphenol G<\/a><\/td><td class=\"oth\">4.72<\/td><td class=\"oth\">250<\/td><td class=\"oth\">311.2<\/td><td class=\"oth\">295.4<\/td><td class=\"oth\">296.4<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol FL\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/3236-71-3\/Bisphenol FL\" target=\"_blank\" rel=\"noopener\">Bisphenol FL<\/a><\/td><td class=\"oth\">4.90<\/td><td class=\"oth\">50.0<\/td><td class=\"oth\">348.8<\/td><td class=\"oth\">256.2<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol BP\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1844-01-5\/Bisphenol BP\" target=\"_blank\" rel=\"noopener\">Bisphenol BP<\/a><\/td><td class=\"oth\">5.14<\/td><td class=\"oth\">50.0<\/td><td class=\"oth\">351.2<\/td><td class=\"oth\">273.3<\/td><td class=\"oth\">274.3<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol M\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/13595-25-0\/Bisphenol M\" target=\"_blank\" rel=\"noopener\">Bisphenol M<\/a><\/td><td class=\"oth\">5.39<\/td><td class=\"oth\">15.0<\/td><td class=\"oth\">345.2<\/td><td class=\"oth\">330.3<\/td><td class=\"oth\">251.4<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol P\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2167-51-3\/Bisphenol P\" target=\"_blank\" rel=\"noopener\">Bisphenol P<\/a><\/td><td class=\"oth\">5.67<\/td><td class=\"oth\">50.0<\/td><td class=\"oth\">345.2<\/td><td class=\"oth\">330.4<\/td><td class=\"oth\">315.3<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol PH\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/24038-68-4\/Bisphenol PH\" target=\"_blank\" rel=\"noopener\">Bisphenol PH<\/a><\/td><td class=\"oth\">6.11<\/td><td class=\"oth\">350<\/td><td class=\"oth\">379.2<\/td><td class=\"oth\">209.4<\/td><td class=\"oth\">364.4<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Biphenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0519\" rel=\"noopener\">cat.# 9309252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>1.8 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>25 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>75:25 Water:methanol<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>2.00-350 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.45<\/td><td>50<\/td><td>50<\/td><\/tr><tr><td>6.50<\/td><td>0.45<\/td><td>10<\/td><td>90<\/td><\/tr><tr><td>6.51<\/td><td>0.45<\/td><td>50<\/td><td>50<\/td><\/tr><tr><td>8.00<\/td><td>0.45<\/td><td>50<\/td><td>50<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0519.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<h3 class=\"wp-block-heading\">Fast Analysis of Sulfur Antibiotics without Coelutions<\/h3>\n\n\n\n<p>Even with high-efficiency UHPLC particles, C18 and ordinary phenyl columns fail to achieve baseline separation of sulfonamides. Not only does the Raptor Biphenyl have the selectivity to easily and completely separate these difficult compounds (Figure 10), but it also does so in well under five minutes!<\/p>\n\n\n\n<p><strong>Figure 10:<\/strong> Sulfonamides pose no problems for analysis even at high linear velocities. Increased retention of early-eluting sulfanilamide also helps limit ionization suppression.<\/p>\n\n\n\n<h3 class=\"wp-block-heading has-text-color has-link-color wp-elements-697ba569a807a8791641c52111bc1dd7\" style=\"color:#68bd45\">Raptor Biphenyl<\/h3>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0530.png\" alt=\"Sulfur Antibiotics on Raptor Biphenyl\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FF0530<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 50px\">t<sub>R<\/sub> (min)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfanilamide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/63-74-1\/Sulfanilamide\" target=\"_blank\" rel=\"noopener\">Sulfanilamide<\/a><\/td><td class=\"oth\">0.41<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfadiazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/68-35-9\/Sulfadiazine\" target=\"_blank\" rel=\"noopener\">Sulfadiazine<\/a><\/td><td class=\"oth\">1.59<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfapyridine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/144-83-2\/Sulfapyridine\" target=\"_blank\" rel=\"noopener\">Sulfapyridine<\/a><\/td><td class=\"oth\">1.84<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfathiazole\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/72-14-0\/Sulfathiazole\" target=\"_blank\" rel=\"noopener\">Sulfathiazole<\/a><\/td><td class=\"oth\">1.96<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfamerazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/127-79-7\/Sulfamerazine\" target=\"_blank\" rel=\"noopener\">Sulfamerazine<\/a><\/td><td class=\"oth\">2.14<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 50px\">t<sub>R<\/sub> (min)<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfamethazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-68-1\/Sulfamethazine\" target=\"_blank\" rel=\"noopener\">Sulfamethazine<\/a><\/td><td class=\"oth\">2.71<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfachlorpyridazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/80-32-0\/Sulfachlorpyridazine\" target=\"_blank\" rel=\"noopener\">Sulfachlorpyridazine<\/a><\/td><td class=\"oth\">3.29<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfadoxine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2447-57-6\/Sulfadoxine\" target=\"_blank\" rel=\"noopener\">Sulfadoxine<\/a><\/td><td class=\"oth\">3.44<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfisoxazole\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/127-69-5\/Sulfisoxazole\" target=\"_blank\" rel=\"noopener\">Sulfisoxazole<\/a><\/td><td class=\"oth\">3.58<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfadimethoxine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/122-11-2\/Sulfadimethoxine\" target=\"_blank\" rel=\"noopener\">Sulfadimethoxine<\/a><\/td><td class=\"oth\">3.98<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfaquinoxaline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/59-40-5\/Sulfaquinoxaline\" target=\"_blank\" rel=\"noopener\">Sulfaquinoxaline<\/a><\/td><td class=\"oth\">4.08<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Biphenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309A5E?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FF0530\" rel=\"noopener\">cat.# 9309A5E<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 3.0 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>20 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>0.1% Formic acid in water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>50 \u00b5g\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.1% Formic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>0.1% Formic acid in acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>1.5<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>2.00<\/td><td>1.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>4.50<\/td><td>1.5<\/td><td>65<\/td><td>35<\/td><\/tr><tr><td>4.51<\/td><td>1.5<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>5.50<\/td><td>1.5<\/td><td>95<\/td><td>5<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>Waters Acquity PDA @ 260, 4.8 nm<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Waters ACQUITY UPLC H-Class<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0530.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<h3 class=\"wp-block-heading has-text-color has-link-color wp-elements-fd8f474b9dc77b0d2993b7ac15c30b7c\" style=\"color:#68bd45\">Competitor B SPP C18<\/h3>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0532.png\" alt=\"Sulfur Antibiotics on Competitor SPP C18\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FF0532<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfanilamide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/63-74-1\/Sulfanilamide\" target=\"_blank\" rel=\"noopener\">Sulfanilamide<\/a><\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfadiazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/68-35-9\/Sulfadiazine\" target=\"_blank\" rel=\"noopener\">Sulfadiazine<\/a><\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfapyridine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/144-83-2\/Sulfapyridine\" target=\"_blank\" rel=\"noopener\">Sulfapyridine<\/a><\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfathiazole\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/72-14-0\/Sulfathiazole\" target=\"_blank\" rel=\"noopener\">Sulfathiazole<\/a><\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfamerazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/127-79-7\/Sulfamerazine\" target=\"_blank\" rel=\"noopener\">Sulfamerazine<\/a><\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfamethazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-68-1\/Sulfamethazine\" target=\"_blank\" rel=\"noopener\">Sulfamethazine<\/a><\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfachlorpyridazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/80-32-0\/Sulfachlorpyridazine\" target=\"_blank\" rel=\"noopener\">Sulfachlorpyridazine<\/a><\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfadoxine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2447-57-6\/Sulfadoxine\" target=\"_blank\" rel=\"noopener\">Sulfadoxine<\/a><\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfisoxazole\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/127-69-5\/Sulfisoxazole\" target=\"_blank\" rel=\"noopener\">Sulfisoxazole<\/a><\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfadimethoxine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/122-11-2\/Sulfadimethoxine\" target=\"_blank\" rel=\"noopener\">Sulfadimethoxine<\/a><\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfaquinoxaline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/59-40-5\/Sulfaquinoxaline\" target=\"_blank\" rel=\"noopener\">Sulfaquinoxaline<\/a><\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Competitor SPP C18<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 3.0 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.6 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>100 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>20 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>0.1% Formic acid in water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>50 \u00b5g\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.1% Formic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>0.1% Formic acid in acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>1.5<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>2.00<\/td><td>1.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>4.50<\/td><td>1.5<\/td><td>65<\/td><td>35<\/td><\/tr><tr><td>4.51<\/td><td>1.5<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>5.50<\/td><td>1.5<\/td><td>95<\/td><td>5<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>Waters Acquity<sup>&#174;<\/sup> PDA @ 260, 4.8 nm<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Waters ACQUITY UPLC H-Class<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0532.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<h3 class=\"wp-block-heading has-text-color has-link-color wp-elements-2345e387ac33019b920ffd7ecba68d23\" style=\"color:#68bd45\">Competitor D FPP UHPLC Phenyl-Hexyl<\/h3>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0536.png\" alt=\"Sulfur Antibiotics on Competitor FPP Phenyl-Hexyl\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FF0536<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfanilamide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/63-74-1\/Sulfanilamide\" target=\"_blank\" rel=\"noopener\">Sulfanilamide<\/a><\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfadiazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/68-35-9\/Sulfadiazine\" target=\"_blank\" rel=\"noopener\">Sulfadiazine<\/a><\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfapyridine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/144-83-2\/Sulfapyridine\" target=\"_blank\" rel=\"noopener\">Sulfapyridine<\/a><\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfathiazole\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/72-14-0\/Sulfathiazole\" target=\"_blank\" rel=\"noopener\">Sulfathiazole<\/a><\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfamerazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/127-79-7\/Sulfamerazine\" target=\"_blank\" rel=\"noopener\">Sulfamerazine<\/a><\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfamethazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-68-1\/Sulfamethazine\" target=\"_blank\" rel=\"noopener\">Sulfamethazine<\/a><\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfachlorpyridazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/80-32-0\/Sulfachlorpyridazine\" target=\"_blank\" rel=\"noopener\">Sulfachlorpyridazine<\/a><\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfadoxine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2447-57-6\/Sulfadoxine\" target=\"_blank\" rel=\"noopener\">Sulfadoxine<\/a><\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfisoxazole\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/127-69-5\/Sulfisoxazole\" target=\"_blank\" rel=\"noopener\">Sulfisoxazole<\/a><\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfadimethoxine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/122-11-2\/Sulfadimethoxine\" target=\"_blank\" rel=\"noopener\">Sulfadimethoxine<\/a><\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sulfaquinoxaline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/59-40-5\/Sulfaquinoxaline\" target=\"_blank\" rel=\"noopener\">Sulfaquinoxaline<\/a><\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Competitor Phenyl-Hexyl<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>1.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>20 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>0.1% Formic acid in water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>50 \u00b5g\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.1% Formic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>0.1% Formic acid in acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.75<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>2.00<\/td><td>0.75<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>4.50<\/td><td>0.75<\/td><td>65<\/td><td>35<\/td><\/tr><tr><td>4.51<\/td><td>0.75<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>5.50<\/td><td>0.75<\/td><td>95<\/td><td>5<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>Waters Acquity<sup>&#174;<\/sup> PDA @ 260, 4.8 nm<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Waters ACQUITY UPLC H-Class<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Flow rate scaled to particle size.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0536.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Experience <em>Selectivity Accelerated<\/em>. Order the Raptor Biphenyl LC Column today.<\/h2>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\" style=\"background-color:#e4f7fa\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<h3 class=\"wp-block-heading has-text-color has-link-color wp-elements-1489b0bd8c7fa6a213f7f4f1cf134d8f\" style=\"color:#02366d\">Want even better performance when analyzing mycotoxins and other metal-sensitive compounds?<\/h3>\n\n\n\n<p>Learn more at&nbsp;<a href=\"https:\/\/www.restek.com\/articles\/accurately-analyze-metal-sensitive-compounds-with-resteks-new-inert-lc-columns\" target=\"_blank\" rel=\"noopener\">www.restek.com\/inert<\/a><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\"><div class=\"wp-block-image\">\n<figure class=\"alignright size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"400\" height=\"123\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/teaser-product-lc-columns-inert-lc-02.png\" alt=\"\" class=\"wp-image-29764\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/teaser-product-lc-columns-inert-lc-02.png 400w, https:\/\/discover.restek.com\/wp-content\/uploads\/teaser-product-lc-columns-inert-lc-02-300x92.png 300w\" sizes=\"auto, (max-width: 400px) 100vw, 400px\" \/><\/figure>\n<\/div><\/div>\n<\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">Related Products<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div 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<\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">EXP Hand-Tight Fitting (Nut w\/Ferrule)<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/25937\">View Product<\/a>\n                                                    <\/div>\n                    <\/div>\n                            <\/div>\n        <\/div>\n        \n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Raptor LC columns combine the speed of superficially porous particles (i.e., SPP or \u201ccore-shell\u201d) with the resolution of highly selective USLC technology. Featuring Restek&#8217;s most popular LC stationary phase, the rugged Raptor Biphenyl is extremely useful for fast separations in bioanalytical testing applications like drug and metabolite analyses, especially those that require a mass spectrometer (MS).<\/p>\n","protected":false},"author":11,"featured_media":7219,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[7],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[2391,2373],"resource-technique":[2299,2301,2302],"hf_cat_post":[650],"ppma_author":[414],"class_list":["post-43713","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles","product-library-lc-columns","product-library-liquid-chromatography-products","resource-technique-liquid-chromatography","resource-technique-mass-spectrometry-ms","resource-technique-ms-ms"],"acf":[],"taxonomy_info":{"category":[{"value":7,"label":"Articles"}],"product-library":[{"value":2391,"label":"LC Columns"},{"value":2373,"label":"Liquid Chromatography Products"}],"resource-technique":[{"value":2299,"label":"Liquid Chromatography"},{"value":2301,"label":"Mass Spectrometry (MS)"},{"value":2302,"label":"MS\/MS"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-GNBR1891-1024x536.jpg",1024,536,true],"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/es\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":7,"name":"Articles","slug":"articles","term_group":0,"term_taxonomy_id":7,"taxonomy":"category","description":"","parent":0,"count":468,"filter":"raw","cat_ID":7,"category_count":468,"category_description":"","cat_name":"Articles","category_nicename":"articles","category_parent":0}],"tag_info":false,"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/43713","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/comments?post=43713"}],"version-history":[{"count":29,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/43713\/revisions"}],"predecessor-version":[{"id":85577,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/43713\/revisions\/85577"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/media\/7219"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/media?parent=43713"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/categories?post=43713"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/tags?post=43713"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/industries-application?post=43713"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/post-badge?post=43713"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/resource-type?post=43713"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/product-library?post=43713"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/resource-technique?post=43713"},{"taxonomy":"hf_cat_post","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/hf_cat_post?post=43713"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/ppma_author?post=43713"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}