{"id":43817,"date":"2020-12-17T00:00:00","date_gmt":"2020-12-17T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/force-performance-lc-columns\/"},"modified":"2026-02-03T14:06:45","modified_gmt":"2026-02-03T14:06:45","slug":"force-performance-lc-columns","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/es\/articles\/gnss2598\/force-performance-lc-columns","title":{"rendered":"Force Performance LC Columns: Apply Force to Your LC Methods"},"content":{"rendered":"\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><style>.wp-block-kadence-advancedbtn.kb-btns43817_866466-fb{gap:var(--global-kb-gap-xs, 0.5rem );justify-content:center;align-items:center;}.kt-btns43817_866466-fb 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class=\"kt-btn-inner-text\"><strong><strong>Browse Force<br>Literature<\/strong><\/strong><\/span><\/a><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><style>.wp-block-kadence-advancedbtn.kb-btns43817_e853f3-b5{gap:var(--global-kb-gap-xs, 0.5rem );justify-content:center;align-items:center;}.kt-btns43817_e853f3-b5 .kt-button{font-weight:normal;font-style:normal;}.kt-btns43817_e853f3-b5 .kt-btn-wrap-0{margin-right:5px;}.wp-block-kadence-advancedbtn.kt-btns43817_e853f3-b5 .kt-btn-wrap-0 .kt-button{color:#555555;border-color:#555555;}.wp-block-kadence-advancedbtn.kt-btns43817_e853f3-b5 .kt-btn-wrap-0 .kt-button:hover, .wp-block-kadence-advancedbtn.kt-btns43817_e853f3-b5 .kt-btn-wrap-0 .kt-button:focus{color:#ffffff;border-color:#444444;}.wp-block-kadence-advancedbtn.kt-btns43817_e853f3-b5 .kt-btn-wrap-0 .kt-button::before{display:none;}.wp-block-kadence-advancedbtn.kt-btns43817_e853f3-b5 .kt-btn-wrap-0 .kt-button:hover, .wp-block-kadence-advancedbtn.kt-btns43817_e853f3-b5 .kt-btn-wrap-0 .kt-button:focus{background:#444444;}<\/style>\n<div class=\"wp-block-kadence-advancedbtn kb-buttons-wrap kb-btns43817_e853f3-b5\"><style>ul.menu .wp-block-kadence-advancedbtn .kb-btn43817_ceb5ca-ed.kb-button{width:initial;}.wp-block-kadence-advancedbtn .kb-btn43817_ceb5ca-ed.kb-button{background:#3278a9;}<\/style><a class=\"kb-button kt-button button kb-btn43817_ceb5ca-ed kt-btn-size-standard kt-btn-width-type-full kb-btn-global-fill  kt-btn-has-text-true kt-btn-has-svg-false  wp-block-kadence-singlebtn\" href=\"https:\/\/www.restek.com\/chromatogram-library?s=force\" target=\"_blank\" rel=\"noreferrer noopener\"><span class=\"kt-btn-inner-text\"><strong><strong>Browse Force<br>Chromatograms<\/strong><\/strong><\/span><\/a><\/div>\n<\/div>\n<\/div>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Long-lasting and reproducible\u2014even with rapid cycling and elevated UHPLC pressures.<\/strong><\/li>\n\n\n\n<li><strong>Fully scalable between HPLC and UHPLC\u2014easily transfer and optimize methods.<\/strong><\/li>\n\n\n\n<li><strong>Premium quality\u2014backed by our 100% Pure Satisfaction guarantee.<\/strong><\/li>\n<\/ul>\n\n\n\n<p>Restek\u2019s new Force performance LC columns give you the power to maximize instrument uptime, increase productivity, and transfer methods across your entire lab, from your trusted-but-aged HPLC to the cutting-edge UHPLC you just plumbed last week. This incredibly rugged and supremely consistent column line represents the culmination of our twenty-plus years in LC dedicated to continually improving our phase chemistries, our lot and lifetime testing, and our bonding and packing procedures.<\/p>\n\n\n\n<p>Meet today\u2019s workflow needs\u2014and prepare for tomorrow\u2019s\u2014by applying Force LC columns to all of your instrument platforms.<\/p>\n\n\n\n<p><em>Available with Restek&#8217;s most-popular and highly selective Biphenyl and FluoroPhenyl phases, as well as a general-purpose C18.<\/em><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td>&nbsp;<\/td><td class=\"has-text-align-center\" data-align=\"center\"><a href=\"https:\/\/www.restek.com\/c\/1774\" target=\"_blank\" data-type=\"link\" data-id=\"https:\/\/www.restek.com\/c\/1774\" rel=\"noreferrer noopener\"><strong>Biphenyl<\/strong><\/a><br> <img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"282\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-01.jpg\" alt=\"Biphenyl structure\" title=\"-\"><\/td><td class=\"has-text-align-center\" data-align=\"center\"><a href=\"https:\/\/www.restek.com\/c\/1775\" target=\"_blank\" data-type=\"link\" data-id=\"https:\/\/www.restek.com\/c\/1775\" rel=\"noreferrer noopener\"><strong>C18<\/strong><\/a><br> <img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"282\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-02.jpg\" alt=\"C18 structure\" title=\"-\"><\/td><td class=\"has-text-align-center\" data-align=\"center\"><a href=\"https:\/\/www.restek.com\/c\/1776\" target=\"_blank\" data-type=\"link\" data-id=\"https:\/\/www.restek.com\/c\/1776\" rel=\"noreferrer noopener\"><strong>FluoroPhenyl<\/strong><\/a><br> <img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"282\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-03.jpg\" alt=\"Fluorophenyl structure\" title=\"-\"><\/td><\/tr><tr><td><strong>USP Phase Code<\/strong><\/td><td>L11<\/td><td class=\"has-text-align-left\" data-align=\"left\">L1<\/td><td class=\"has-text-align-left\" data-align=\"left\">L43<\/td><\/tr><tr><td><strong>Stationary Phase Category<\/strong><\/td><td>Phenyl<\/td><td class=\"has-text-align-left\" data-align=\"left\">C18, octadecylsilane<\/td><td class=\"has-text-align-left\" data-align=\"left\">Pentafluorophenyl propyl<\/td><\/tr><tr><td><strong>Ligand Type<\/strong><\/td><td>Biphenyl<\/td><td class=\"has-text-align-left\" data-align=\"left\">End-capped C18<\/td><td class=\"has-text-align-left\" data-align=\"left\">Fluorophenyl<\/td><\/tr><tr><td><strong>Particle Size<\/strong><\/td><td>1.8 \u00b5m, 3 \u03bcm, or 5 \u03bcm<br>fully porous<\/td><td class=\"has-text-align-left\" data-align=\"left\">1.8 \u00b5m, 3 \u03bcm, or 5 \u03bcm<br>fully porous<\/td><td class=\"has-text-align-left\" data-align=\"left\">1.8 \u00b5m, 3 \u03bcm, or 5 \u03bcm<br>fully porous<\/td><\/tr><tr><td><strong>Pore Size<\/strong><\/td><td>100 \u00c5<\/td><td class=\"has-text-align-left\" data-align=\"left\">100 \u00c5<\/td><td class=\"has-text-align-left\" data-align=\"left\">100 \u00c5<\/td><\/tr><tr><td><strong>Surface Area<\/strong><\/td><td>300 m<sup>2<\/sup>\/g<\/td><td class=\"has-text-align-left\" data-align=\"left\">300 m<sup>2<\/sup>\/g<\/td><td class=\"has-text-align-left\" data-align=\"left\">300 m<sup>2<\/sup>\/g<\/td><\/tr><tr><td><strong>Carbon Load<\/strong><\/td><td>15%<\/td><td class=\"has-text-align-left\" data-align=\"left\">20%<\/td><td class=\"has-text-align-left\" data-align=\"left\">10%<\/td><\/tr><tr><td><strong>End-Cap<\/strong><\/td><td>yes<\/td><td class=\"has-text-align-left\" data-align=\"left\">yes<\/td><td class=\"has-text-align-left\" data-align=\"left\">no<\/td><\/tr><tr><td><strong>pH Range<\/strong><\/td><td>2.0 to 8.0<\/td><td class=\"has-text-align-left\" data-align=\"left\">2.0 to 8.0<\/td><td class=\"has-text-align-left\" data-align=\"left\">2.0 to 8.0<\/td><\/tr><tr><td><strong>Maximum Temperature<\/strong><\/td><td>80 \u00b0C<\/td><td class=\"has-text-align-left\" data-align=\"left\">80 \u00b0C<\/td><td class=\"has-text-align-left\" data-align=\"left\">80 \u00b0C<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">Apply Force LC Columns to your LC Methods\u2026for Longer Column Life<\/h3>\n\n\n\n<p>Elevated pressures and rapid pressure cycling put extreme stress on your LC column and shorten its life. And when the demand to increase lab productivity meets the growing pressure limits of modern LCs and the faster cycle times of new methods, many competitor columns simply can\u2019t survive. Force LC columns from Restek are designed and manufactured to handle high-pressure, high-stress conditions. Whether you\u2019re running a 3 or 5 \u03bcm column on an older HPLC or a 1.8 \u03bcm on the newest UHPLC in your lab (Figures 1 &amp; 2), your method will give you the same separation from one injection to the next when you trust your workflow to the extended lifetime of a Force LC column.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 1:<\/strong>\u00a0At over 10,000 psi, you may be used to seeing a drop in efficiency, but Force LC columns will take the pressure.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"753\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-04-1024x753.jpg\" alt=\"Chart of percentage efficiency vs number of injections on 1.8 micron C18 Force LC Columns at 12,000 psi\" class=\"wp-image-23086\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-04-1024x753.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-04-300x221.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-04-768x565.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-04.jpg 1400w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div>\n\n\n<p class=\"has-text-align-center\"><strong><em>1000 injections and still going strong at high pressure.<\/em><\/strong><\/p>\n\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 2:<\/strong>\u00a0Rapid changes in pressure place more stress on a column than even high pressures can, but Force LC columns will handle repeated analyses with no change in separation. You get stable retention times and peak shapes even with rapid cycling!<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0568.png\" alt=\"Injection Reproducibility for Isoflavones on Force C18 by UHPLC-MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FF0568<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 50px\">SIR (m\/z)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Daidzin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/552-66-9\/Daidzin\" target=\"_blank\" rel=\"noopener\">Daidzin<\/a><\/td><td class=\"oth\">417.2<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Genistin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/529-59-9\/Genistin\" target=\"_blank\" rel=\"noopener\">Genistin<\/a><\/td><td class=\"oth\">433.2<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Daidzein\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/468-66-8\/Daidzein\" target=\"_blank\" rel=\"noopener\">Daidzein<\/a><\/td><td class=\"oth\">255.1<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Genistein\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/446-72-0\/Genistein\" target=\"_blank\" rel=\"noopener\">Genistein<\/a><\/td><td class=\"oth\">271.1<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Force C18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9634252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FF0568\" rel=\"noopener\">cat.# 9634252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>1.8 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>UltraShield UHPLC precolumn filter 0.2 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/25810?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FF0568\" rel=\"noopener\">cat.# 25810<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>50 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Custom mix<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>500 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water + 0.1% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile + 0.1% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.6<\/td><td>85<\/td><td>15<\/td><\/tr><tr><td>1.50<\/td><td>0.6<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>1.51<\/td><td>0.6<\/td><td>85<\/td><td>15<\/td><\/tr><tr><td>3.00<\/td><td>0.6<\/td><td>85<\/td><td>15<\/td><\/tr><\/tbody><\/table><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Max Pressure:<\/th><td>500 bar<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Interface:<\/th><td>ESI+ <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0568.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">Apply Force LC Columns to your LC Methods\u2026for Improved Reproducibility<\/h3>\n\n\n\n<p>Once you set up and validate a workflow, you move on to developing the next new method. You don\u2019t have time to repeatedly revisit past methods because your chosen column is giving you different results with each lot. Peak shapes and retention times need to be maintained over the lifetime of your workflow to ensure consistent results, reduce unplanned downtime, and preserve your own productivity. Force LC columns have the lot-to-lot reproducibility you need to rely on (Figure 3)\u2014backed by Restek\u2019s strict QC system and our 100% Pure Satisfaction guaran\u00adtee\u2014so that you are free to focus your energy on what\u2019s next.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 3:<\/strong>\u00a0Rapid changes in pressure place more stress on a column than even high pressures can, but Force LC columns will handle repeated analyses with no change in separation. You get stable retention times and peak shapes even with rapid cycling!<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<p class=\"has-medium-font-size\"><strong>Force C18<\/strong><\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0567.png\" alt=\"Lot Comparison for Isoflavones on Force C18 by UHPLC-MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FF0567<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 50px\">SIR (m\/z)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Daidzin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/552-66-9\/Daidzin\" target=\"_blank\" rel=\"noopener\">Daidzin<\/a><\/td><td class=\"oth\">417.2<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Genistin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/529-59-9\/Genistin\" target=\"_blank\" rel=\"noopener\">Genistin<\/a><\/td><td class=\"oth\">433.2<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Daidzein\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/468-66-8\/Daidzein\" target=\"_blank\" rel=\"noopener\">Daidzein<\/a><\/td><td class=\"oth\">255.1<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Genistein\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/446-72-0\/Genistein\" target=\"_blank\" rel=\"noopener\">Genistein<\/a><\/td><td class=\"oth\">271.1<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Force C18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9634252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FF0567\" rel=\"noopener\">cat.# 9634252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>1.8 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>50 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Custom mix<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>500 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water + 0.1% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile + 0.1% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.6<\/td><td>85<\/td><td>15<\/td><\/tr><tr><td>1.50<\/td><td>0.6<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>1.51<\/td><td>0.6<\/td><td>85<\/td><td>15<\/td><\/tr><tr><td>3.00<\/td><td>0.6<\/td><td>85<\/td><td>15<\/td><\/tr><\/tbody><\/table><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Max Pressure:<\/th><td>500 bar<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Interface:<\/th><td>ESI+ <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0567.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<p class=\"has-medium-font-size\"><strong>Force FluoroPhenyl<\/strong><\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0510.png\" alt=\"Lot Comparison for Nitrofurans on Force FluoroPhenyl by UHPLC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0510<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nitrofurantoin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/67-20-9\/Nitrofurantoin\" target=\"_blank\" rel=\"noopener\">Nitrofurantoin<\/a><\/td><td class=\"oth\">239.1<\/td><td class=\"oth\">121.9<\/td><td class=\"oth\">95.0<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nitrofurazone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/59-87-0\/Nitrofurazone\" target=\"_blank\" rel=\"noopener\">Nitrofurazone<\/a><\/td><td class=\"oth\">199.1<\/td><td class=\"oth\">107.9<\/td><td class=\"oth\">54.0<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Furazolidone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/67-45-8\/Furazolidone\" target=\"_blank\" rel=\"noopener\">Furazolidone<\/a><\/td><td class=\"oth\">226.2<\/td><td class=\"oth\">95.0<\/td><td class=\"oth\">67.0<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nifuroxazide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/965-52-6\/Nifuroxazide\" target=\"_blank\" rel=\"noopener\">Nifuroxazide<\/a><\/td><td class=\"oth\">276.2<\/td><td class=\"oth\">121.0<\/td><td class=\"oth\">93.0<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Force FluoroPhenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9639252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0510\" rel=\"noopener\">cat.# 9639252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>1.8 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>50 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.1% Acetic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>70<\/td><td>30<\/td><\/tr><tr><td>1.50<\/td><td>0.4<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>1.51<\/td><td>0.4<\/td><td>70<\/td><td>30<\/td><\/tr><tr><td>3.50<\/td><td>0.4<\/td><td>70<\/td><td>30<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0510.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">Apply Force LC Columns to your LC Methods\u2026for Complete Scalability<\/h3>\n\n\n\n<p>To accelerate time to market, analysts will often develop methods on fast UHPLC instruments using sub-2 \u03bcm particle columns. But the methods must then be scaled to match the analysis time and pressure limitations of the traditional HPLCs and the 3 or 5 \u03bcm columns that will actually be doing the work. To make this transition easier and ensure consistent results, Force LC columns are available in three particle sizes that are manufactured on the same silica support with the same properties to reliably and easily provide the same separation on any instrument platform (Figure 4).<\/p>\n\n\n\n<p>And, of course, it works both ways: fully scalable Force LC columns also make it easy to update conventional HPLC methods to UHPLC instruments to increase sample throughput and reduce solvent consumption and waste disposal costs.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 4:<\/strong>\u00a0With identical isocratic flow rates, 5, 3, and 1.8 \u03bcm Force columns still offer the same results. (If using a gradient, simple equations can help you adjust slope and time points.) Scaling doesn\u2019t get easier than that! Notice that peak shape and plate number improve as particle size decreases\u2014ideal for increased MS sensitivity.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0574.png\" alt=\"Scalability of Force C18 Using Reversed Phase Test Mix\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_GN0574<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 50px\">t<sub>R<\/sub> (min)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Uracil\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/66-22-8\/Uracil\" target=\"_blank\" rel=\"noopener\">Uracil<\/a><\/td><td class=\"oth\">0.456<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Toluene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/108-88-3\/Toluene\" target=\"_blank\" rel=\"noopener\">Toluene<\/a><\/td><td class=\"oth\">2.904<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Naphthalene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/91-20-3\/Naphthalene\" target=\"_blank\" rel=\"noopener\">Naphthalene<\/a><\/td><td class=\"oth\">3.939<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Biphenyl\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/92-52-4\/Biphenyl\" target=\"_blank\" rel=\"noopener\">Biphenyl<\/a><\/td><td class=\"oth\">5.971<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Force, 100 x 2.1 mm (see above for particle sizes)<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>UHPLC RP test mix<\/td><\/tr><tr><td><\/td><\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water:acetonitrile (50:50)<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>45<\/td><td>55<\/td><\/tr><tr><td>8.00<\/td><td>0.4<\/td><td>45<\/td><td>55<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>PDA @ 254 nm<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0574.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">Force LC Columns at Work: Time-Tested Restek Biphenyl Phase<\/h3>\n\n\n\n<h4 class=\"wp-block-heading\"><em>The established choice for pharmaceutical testing since 2005<\/em><\/h4>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-a1bf2daf wp-block-columns-is-layout-flex\" style=\"border-bottom-width:1px;background-color:#e5f7fa;margin-bottom:0;padding-bottom:30px\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<ul class=\"wp-block-list\">\n<li>Separates compounds that other phenyl and C18 chemistries can\u2019t.<\/li>\n\n\n\n<li>Allows the use of simple, MS-friendly mobile phases.<\/li>\n\n\n\n<li>Restek\u2019s most popular LC phase.<\/li>\n<\/ul>\n\n\n\n<p><strong>Properties:<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Increased retention for dipolar, unsaturated, or conjugated solutes.<\/li>\n\n\n\n<li>Enhanced selectivity when used with methanolic mobile phase.<\/li>\n\n\n\n<li>Ideal for increasing sensitivity and selectivity in LC-MS analyses.<\/li>\n<\/ul>\n\n\n\n<p><strong><strong>Switch to a Biphenyl when:<\/strong><\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Limited selectivity is observed on a C18.<\/li>\n\n\n\n<li>You need to increase retention of hydrophilic aromatics.<\/li>\n<\/ul>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\"><style>.kb-image43817_eb7938-81.kb-image-is-ratio-size, .kb-image43817_eb7938-81 .kb-image-is-ratio-size{max-width:185px;width:100%;}.wp-block-kadence-column > .kt-inside-inner-col > .kb-image43817_eb7938-81.kb-image-is-ratio-size, .wp-block-kadence-column > .kt-inside-inner-col > .kb-image43817_eb7938-81 .kb-image-is-ratio-size{align-self:unset;}.kb-image43817_eb7938-81 figure{max-width:185px;}.kb-image43817_eb7938-81 .image-is-svg, .kb-image43817_eb7938-81 .image-is-svg img{width:100%;}.kb-image43817_eb7938-81 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43817_eb7938-81\"><figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"254\" height=\"657\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-05.png\" alt=\"\" class=\"kb-img wp-image-23096\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-05.png 254w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-05-116x300.png 116w\" sizes=\"auto, (max-width: 254px) 100vw, 254px\" \/><\/figure><\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-b3f54ac0 wp-block-columns-is-layout-flex\" style=\"border-bottom-width:1px;background-color:#e5f7fa;margin-top:0;margin-bottom:0px;padding-top:30px\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h3 class=\"wp-block-heading\">Column Interaction Profile<\/h3>\n\n\n\n<p><strong>Defining Solute Interactions:<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Polarizability<\/li>\n\n\n\n<li>Dispersion<\/li>\n<\/ul>\n\n\n\n<p><strong><strong>Complementary Solute Interaction:<\/strong><\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Cation exchange<\/li>\n<\/ul>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\"><style>.kb-image43817_8c4b29-1c.kb-image-is-ratio-size, .kb-image43817_8c4b29-1c .kb-image-is-ratio-size{max-width:246px;width:100%;}.wp-block-kadence-column > .kt-inside-inner-col > .kb-image43817_8c4b29-1c.kb-image-is-ratio-size, .wp-block-kadence-column > .kt-inside-inner-col > .kb-image43817_8c4b29-1c .kb-image-is-ratio-size{align-self:unset;}.kb-image43817_8c4b29-1c figure{max-width:246px;}.kb-image43817_8c4b29-1c .image-is-svg, .kb-image43817_8c4b29-1c .image-is-svg img{width:100%;}.kb-image43817_8c4b29-1c .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43817_8c4b29-1c\"><figure class=\"aligncenter size-medium\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/\/figure-article-GNSS2598-06-300x300.png\" alt=\"\" class=\"kb-img wp-image-23102\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-06-300x300.png 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-06-150x150.png 150w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-06.png 392w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/figure><\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-0bfe8ce4 wp-block-columns-is-layout-flex\" style=\"border-bottom-width:1px;background-color:#e5f7fa;margin-top:0;padding-top:30px\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h3 class=\"wp-block-heading\">Solute Retention Profile<\/h3>\n\n\n\n<p><strong><strong>Target Analyte Structures:<\/strong><\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Aromatic<\/li>\n\n\n\n<li>Dipolar<\/li>\n<\/ul>\n\n\n\n<p><strong><strong>Target Analyte Functionalities:<\/strong><\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Hydrophilic aromatics<\/li>\n\n\n\n<li>Strong dipoles<\/li>\n\n\n\n<li>Lewis acids<\/li>\n\n\n\n<li>Dipolar, unsaturated, or conjugated compounds<\/li>\n\n\n\n<li>Fused-ring compounds with electron withdrawing groups<\/li>\n<\/ul>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\"><style>.kb-image43817_b1925f-30 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43817_b1925f-30\"><figure class=\"aligncenter size-medium_large\"><img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"249\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/\/figure-article-GNSS2598-07-768x249.png\" alt=\"\" class=\"kb-img wp-image-23114\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-07-768x249.png 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-07-300x97.png 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-07.png 900w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/figure><\/div>\n<\/div>\n<\/div>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 5:<\/strong>\u00a0Whether for therapeutic drug monitoring or toxicology, Force Biphenyl columns were made for applications where fast, reliable identi\u00adfication of drugs and metabolites could be a matter of life and death.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0677.png\" alt=\"Morphine and Metabolites on Force Biphenyl by UHPLC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_CF0677<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Morphine-3-\u03b2-D-glucuronide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/20290-09-9\/Morphine-3-\u03b2-D-glucuronide\" target=\"_blank\" rel=\"noopener\">Morphine-3-\u03b2-D-glucuronide<\/a><\/td><td class=\"oth\">0.70<\/td><td class=\"oth\">462.1<\/td><td class=\"oth\">286.0<\/td><td class=\"oth\">152.1<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Morphine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-27-2\/Morphine\" target=\"_blank\" rel=\"noopener\">Morphine<\/a><\/td><td class=\"oth\">1.26<\/td><td class=\"oth\">286.1<\/td><td class=\"oth\">164.9<\/td><td class=\"oth\">152.0<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Morphine-6-\u03b2-D-glucuronide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/20290-10-2\/Morphine-6-\u03b2-D-glucuronide\" target=\"_blank\" rel=\"noopener\">Morphine-6-\u03b2-D-glucuronide<\/a><\/td><td class=\"oth\">1.34<\/td><td class=\"oth\">462.1<\/td><td class=\"oth\">286.0<\/td><td class=\"oth\">152.1<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Morphine-N-oxide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/639-46-3\/Morphine-N-oxide\" target=\"_blank\" rel=\"noopener\">Morphine-N-oxide<\/a><\/td><td class=\"oth\">1.41<\/td><td class=\"oth\">302.1<\/td><td class=\"oth\">284.9<\/td><td class=\"oth\">161.9<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 6-Acetylmorphine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2784-73-8\/6-Acetylmorphine\" target=\"_blank\" rel=\"noopener\">6-Acetylmorphine<\/a><\/td><td class=\"oth\">1.86<\/td><td class=\"oth\">328.1<\/td><td class=\"oth\">211.1<\/td><td class=\"oth\">165.0<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Force Biphenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9629252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0677\" rel=\"noopener\">cat.# 9629252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>1.8 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>0.1% Formic acid in water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>50 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.1% Formic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>0.1% Formic acid in methanol <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>85<\/td><td>15<\/td><\/tr><tr><td>0.50<\/td><td>0.5<\/td><td>85<\/td><td>15<\/td><\/tr><tr><td>2.00<\/td><td>0.5<\/td><td>30<\/td><td>70<\/td><\/tr><tr><td>2.01<\/td><td>0.5<\/td><td>85<\/td><td>15<\/td><\/tr><tr><td>4.00<\/td><td>0.5<\/td><td>85<\/td><td>15<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0677.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 6:<\/strong>\u00a0Accurate quantification is critical for cardiac drugs due to their narrow therapeutic range, and the Restek Biphenyl phase on a Force LC column is the ideal choice.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0675.png\" alt=\"Cardiac Drugs on Force Biphenyl by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_CF0675<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Polarity<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Digoxin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/20830-75-5\/Digoxin\" target=\"_blank\" rel=\"noopener\">Digoxin<\/a><\/td><td class=\"oth\">0.95<\/td><td class=\"oth\">798.4<\/td><td class=\"oth\">651.4<\/td><td class=\"oth\">355.1<\/td><td class=\"oth\">+<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Flecainide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/54143-55-4\/Flecainide\" target=\"_blank\" rel=\"noopener\">Flecainide<\/a><\/td><td class=\"oth\">1.28<\/td><td class=\"oth\">415.3<\/td><td class=\"oth\">397.9<\/td><td class=\"oth\">300.9<\/td><td class=\"oth\">+<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Furosemide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/54-31-9\/Furosemide\" target=\"_blank\" rel=\"noopener\">Furosemide<\/a><\/td><td class=\"oth\">1.32<\/td><td class=\"oth\">329.0<\/td><td class=\"oth\">205.2<\/td><td class=\"oth\">285.1<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Verapamil\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/52-53-9\/Verapamil\" target=\"_blank\" rel=\"noopener\">Verapamil<\/a><\/td><td class=\"oth\">1.68<\/td><td class=\"oth\">455.1<\/td><td class=\"oth\">165.2<\/td><td class=\"oth\">150.0<\/td><td class=\"oth\">+<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Digitoxin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/71-63-6\/Digitoxin\" target=\"_blank\" rel=\"noopener\">Digitoxin<\/a><\/td><td class=\"oth\">1.85<\/td><td class=\"oth\">782.4<\/td><td class=\"oth\">635.2<\/td><td class=\"oth\">243.1<\/td><td class=\"oth\">+<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Amiodarone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1951-25-3\/Amiodarone\" target=\"_blank\" rel=\"noopener\">Amiodarone<\/a><\/td><td class=\"oth\">3.17<\/td><td class=\"oth\">646.1<\/td><td class=\"oth\">58.1<\/td><td class=\"oth\">86.2<\/td><td class=\"oth\">+<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Force  Biphenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9629252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0675\" rel=\"noopener\">cat.# 9629252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>1.8 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water:acetonitrile (90:10)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>100 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water + 5 mM ammonium formate + 0.1% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile + 0.1% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.3<\/td><td>60<\/td><td>40<\/td><\/tr><tr><td>3.50<\/td><td>0.3<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>3.51<\/td><td>0.3<\/td><td>60<\/td><td>40<\/td><\/tr><tr><td>5.5<\/td><td>0.3<\/td><td>60<\/td><td>40<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+\/ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>Scheduled MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0675.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">Force LC Columns at Work: General-Purpose Restek C18 Phase<\/h3>\n\n\n\n<h4 class=\"wp-block-heading\"><em>Force column dependability, scalability, and quality in a C18<\/em><\/h4>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-a1bf2daf wp-block-columns-is-layout-flex\" style=\"border-bottom-width:1px;background-color:#e5f7fa;margin-bottom:0;padding-bottom:30px\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<ul class=\"wp-block-list\">\n<li>Wide pH range provides excellent data quality for many applications.<\/li>\n\n\n\n<li>High carbon load (20%) offers high hydrophobic retention.<\/li>\n<\/ul>\n\n\n\n<p><strong><strong>Properties<\/strong><\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Compatible with moderately acidic to neutral mobile phases (pH 2\u20138).<\/li>\n\n\n\n<li>Excellent data quality in food, environmental, bioanalytical, and other applications.<\/li>\n<\/ul>\n\n\n\n<p><strong><strong>Switch to a C18 when:<\/strong><\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>You need a general-purpose column for reversed-phase chromatography.<\/li>\n\n\n\n<li>You need to increase retention of hydrophobic compounds.<\/li>\n<\/ul>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\"><style>.kb-image43817_5721c8-77.kb-image-is-ratio-size, .kb-image43817_5721c8-77 .kb-image-is-ratio-size{max-width:200px;width:100%;}.wp-block-kadence-column > .kt-inside-inner-col > .kb-image43817_5721c8-77.kb-image-is-ratio-size, .wp-block-kadence-column > .kt-inside-inner-col > .kb-image43817_5721c8-77 .kb-image-is-ratio-size{align-self:unset;}.kb-image43817_5721c8-77 figure{max-width:200px;}.kb-image43817_5721c8-77 .image-is-svg, .kb-image43817_5721c8-77 .image-is-svg img{width:100%;}.kb-image43817_5721c8-77 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43817_5721c8-77\"><figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"306\" height=\"684\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-08.png\" alt=\"\" class=\"kb-img wp-image-23120\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-08.png 306w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-08-134x300.png 134w\" sizes=\"auto, (max-width: 306px) 100vw, 306px\" \/><\/figure><\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-b3f54ac0 wp-block-columns-is-layout-flex\" style=\"border-bottom-width:1px;background-color:#e5f7fa;margin-top:0;margin-bottom:0px;padding-top:30px\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h3 class=\"wp-block-heading\">Column Interaction Profile<\/h3>\n\n\n\n<p><strong><strong>Defining Solute Interaction:<\/strong><\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Dispersion<\/li>\n<\/ul>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\"><style>.kb-image43817_edd626-08.kb-image-is-ratio-size, .kb-image43817_edd626-08 .kb-image-is-ratio-size{max-width:246px;width:100%;}.wp-block-kadence-column > .kt-inside-inner-col > .kb-image43817_edd626-08.kb-image-is-ratio-size, .wp-block-kadence-column > .kt-inside-inner-col > .kb-image43817_edd626-08 .kb-image-is-ratio-size{align-self:unset;}.kb-image43817_edd626-08 figure{max-width:246px;}.kb-image43817_edd626-08 .image-is-svg, .kb-image43817_edd626-08 .image-is-svg img{width:100%;}.kb-image43817_edd626-08 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43817_edd626-08\"><figure class=\"aligncenter size-medium\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/\/figure-article-GNSS2598-09-300x300.png\" alt=\"\" class=\"kb-img wp-image-23126\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-09-300x300.png 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-09-150x150.png 150w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-09.png 390w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/figure><\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-0bfe8ce4 wp-block-columns-is-layout-flex\" style=\"border-bottom-width:1px;background-color:#e5f7fa;margin-top:0;padding-top:30px\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h3 class=\"wp-block-heading\">Solute Retention Profile<\/h3>\n\n\n\n<p><strong><strong>Target Analyte Structure:<\/strong><\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Hydrocarbons<\/li>\n<\/ul>\n\n\n\n<p><strong><strong>Target Analyte Functionality:<\/strong><\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Hydrophobic compounds<\/li>\n<\/ul>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\"><style>.kb-image43817_a3c962-e5.kb-image-is-ratio-size, .kb-image43817_a3c962-e5 .kb-image-is-ratio-size{max-width:430px;width:100%;}.wp-block-kadence-column > .kt-inside-inner-col > .kb-image43817_a3c962-e5.kb-image-is-ratio-size, .wp-block-kadence-column > .kt-inside-inner-col > .kb-image43817_a3c962-e5 .kb-image-is-ratio-size{align-self:unset;}.kb-image43817_a3c962-e5 figure{max-width:430px;}.kb-image43817_a3c962-e5 .image-is-svg, .kb-image43817_a3c962-e5 .image-is-svg img{width:100%;}.kb-image43817_a3c962-e5 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43817_a3c962-e5\"><figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"900\" height=\"198\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-10.png\" alt=\"\" class=\"kb-img wp-image-23138\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-10.png 900w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-10-300x66.png 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-10-768x169.png 768w\" sizes=\"auto, (max-width: 900px) 100vw, 900px\" \/><\/figure><\/div>\n<\/div>\n<\/div>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 7:<\/strong>\u00a0A C18 is a common first choice for method developers, and as shown here for these isoflavones commonly found in nutraceuticals, a Force C18 column is the C18 to choose.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0566.png\" alt=\"Analysis of Isoflavones on Force C18 by UHPLC-MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FF0566<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 50px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 50px\">SIR (m\/z)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Daidzin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/552-66-9\/Daidzin\" target=\"_blank\" rel=\"noopener\">Daidzin<\/a><\/td><td class=\"oth\">0.622<\/td><td class=\"oth\">417.2<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Genistin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/529-59-9\/Genistin\" target=\"_blank\" rel=\"noopener\">Genistin<\/a><\/td><td class=\"oth\">0.949<\/td><td class=\"oth\">433.2<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Daidzein\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/468-66-8\/Daidzein\" target=\"_blank\" rel=\"noopener\">Daidzein<\/a><\/td><td class=\"oth\">1.174<\/td><td class=\"oth\">255.1<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Genistein\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/446-72-0\/Genistein\" target=\"_blank\" rel=\"noopener\">Genistein<\/a><\/td><td class=\"oth\">1.314<\/td><td class=\"oth\">271.1<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Force C18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9634252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FF0566\" rel=\"noopener\">cat.# 9634252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>1.8 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>50 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Custom mix<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>500 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water + 0.1% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile + 0.1% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.6<\/td><td>85<\/td><td>15<\/td><\/tr><tr><td>1.50<\/td><td>0.6<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>1.51<\/td><td>0.6<\/td><td>85<\/td><td>15<\/td><\/tr><tr><td>3.00<\/td><td>0.6<\/td><td>85<\/td><td>15<\/td><\/tr><\/tbody><\/table><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Max Pressure:<\/th><td>500 bar<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Interface:<\/th><td>ESI+ <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0566.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\" style=\"background-color:#e5f7fa\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h2 class=\"wp-block-heading\"><strong><strong><strong>Tech Tip: Small-Particle Column Protection<\/strong><\/strong><\/strong><\/h2>\n\n\n\n<p>Protecting your column is always recommended. Restek offers EXP guard column cartridges for our 3 and 5 \u00b5m Force LC columns, but for 1.8 \u00b5m columns, where the additional volume of a guard is an issue, reach for the UltraShield UHPLC PreColumn filter with 0.2 \u03bcm frit. Its minimal dead volume (1 \u00b5L) makes it recommended for UHPLC up to 15,000 psi.<\/p>\n<\/div>\n<\/div>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 8:<\/strong>\u00a0You can pair a 1.8 \u03bcm Force column with an UltraShield UHPLC Pre\u00adColumn filter to prolong column lifetime\u2014without significantly altering retention times.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0569.png\" alt=\"Comparison of Isoflavones on Force C18 with and without UltraShield by UHPLC-MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FF0569<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 50px\">SIR (m\/z)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Daidzin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/552-66-9\/Daidzin\" target=\"_blank\" rel=\"noopener\">Daidzin<\/a><\/td><td class=\"oth\">417.2<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Genistin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/529-59-9\/Genistin\" target=\"_blank\" rel=\"noopener\">Genistin<\/a><\/td><td class=\"oth\">433.2<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Daidzein\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/468-66-8\/Daidzein\" target=\"_blank\" rel=\"noopener\">Daidzein<\/a><\/td><td class=\"oth\">255.1<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Genistein\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/446-72-0\/Genistein\" target=\"_blank\" rel=\"noopener\">Genistein<\/a><\/td><td class=\"oth\">271.1<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Force C18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9634252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FF0569\" rel=\"noopener\">cat.# 9634252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>1.8 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>UltraShield UHPLC precolumn filter 0.2 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/25810?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FF0569\" rel=\"noopener\">cat.# 25810<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>50 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Custom mix<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>500 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water + 0.1% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile + 0.1% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.6<\/td><td>85<\/td><td>15<\/td><\/tr><tr><td>1.50<\/td><td>0.6<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>1.51<\/td><td>0.6<\/td><td>85<\/td><td>15<\/td><\/tr><tr><td>3.00<\/td><td>0.6<\/td><td>85<\/td><td>15<\/td><\/tr><\/tbody><\/table><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Max Pressure:<\/th><td>500 bar<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Interface:<\/th><td>ESI+ <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0569.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">Force LC Columns at Work: Reliably Versatile Restek FluoroPhenyl Phase<\/h3>\n\n\n\n<h4 class=\"wp-block-heading\"><em>Get the power of HILIC and RP modes in one LC column<\/em><\/h4>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-a1bf2daf wp-block-columns-is-layout-flex\" style=\"border-bottom-width:1px;background-color:#e5f7fa;margin-bottom:0;padding-bottom:30px\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<ul class=\"wp-block-list\">\n<li>Retains hydrophobic, polar, and aromatic compounds.<\/li>\n\n\n\n<li>Has orthogonal selectivity to a C18.<\/li>\n\n\n\n<li>Exceptionally reproducible\u2014predictable performance from every column.<\/li>\n<\/ul>\n\n\n\n<p><strong>Properties:<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Capable of both reversed-phase and HILIC separations.<\/li>\n\n\n\n<li>Ideal for increasing sensitivity and selectivity in LC-MS analyses.<\/li>\n\n\n\n<li>Offers increased retention for charged bases.<\/li>\n<\/ul>\n\n\n\n<p><strong>Switch to FluoroPhenyl when:<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Limited retention and selectivity are observed on a C18 for basic compounds.<\/li>\n\n\n\n<li>You need increased retention of hydrophilic compounds.<\/li>\n<\/ul>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\"><style>.kb-image43817_675803-ff.kb-image-is-ratio-size, .kb-image43817_675803-ff .kb-image-is-ratio-size{max-width:200px;width:100%;}.wp-block-kadence-column > .kt-inside-inner-col > .kb-image43817_675803-ff.kb-image-is-ratio-size, .wp-block-kadence-column > .kt-inside-inner-col > .kb-image43817_675803-ff .kb-image-is-ratio-size{align-self:unset;}.kb-image43817_675803-ff figure{max-width:200px;}.kb-image43817_675803-ff .image-is-svg, .kb-image43817_675803-ff .image-is-svg img{width:100%;}.kb-image43817_675803-ff .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43817_675803-ff\"><figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"244\" height=\"598\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-11.png\" alt=\"\" class=\"kb-img wp-image-23140\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-11.png 244w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-11-122x300.png 122w\" sizes=\"auto, (max-width: 244px) 100vw, 244px\" \/><\/figure><\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-b3f54ac0 wp-block-columns-is-layout-flex\" style=\"border-bottom-width:1px;background-color:#e5f7fa;margin-top:0;margin-bottom:0px;padding-top:30px\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h3 class=\"wp-block-heading\">Column Interaction Profile<\/h3>\n\n\n\n<p><strong><strong>Defining Solute Interactions:<\/strong><\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Cation exchange<\/li>\n<\/ul>\n\n\n\n<p><strong>Complementary Solute Interactions:<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Polarizability<\/li>\n\n\n\n<li>Dispersion<\/li>\n<\/ul>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\"><style>.kb-image43817_144c57-6c.kb-image-is-ratio-size, .kb-image43817_144c57-6c .kb-image-is-ratio-size{max-width:246px;width:100%;}.wp-block-kadence-column > .kt-inside-inner-col > .kb-image43817_144c57-6c.kb-image-is-ratio-size, .wp-block-kadence-column > .kt-inside-inner-col > .kb-image43817_144c57-6c .kb-image-is-ratio-size{align-self:unset;}.kb-image43817_144c57-6c figure{max-width:246px;}.kb-image43817_144c57-6c .image-is-svg, .kb-image43817_144c57-6c .image-is-svg img{width:100%;}.kb-image43817_144c57-6c .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43817_144c57-6c\"><figure class=\"aligncenter size-medium\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-12-300x300.png\" alt=\"\" class=\"kb-img wp-image-23146\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-12-300x300.png 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-12-150x150.png 150w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-12.png 392w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/figure><\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-0bfe8ce4 wp-block-columns-is-layout-flex\" style=\"border-bottom-width:1px;background-color:#e5f7fa;margin-top:0;padding-top:30px\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h3 class=\"wp-block-heading\">Solute Retention Profile<\/h3>\n\n\n\n<p><strong><strong>Target Analyte Structure:<\/strong><\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Nitrogen<\/li>\n<\/ul>\n\n\n\n<p><strong><strong>Target Analyte Functionality:<\/strong><\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Protonated amines<\/li>\n\n\n\n<li>Quaternary ammonium compounds<\/li>\n\n\n\n<li>Positively charged moieties<\/li>\n\n\n\n<li>Lewis bases<\/li>\n<\/ul>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\"><style>.kb-image43817_0076d0-d8.kb-image-is-ratio-size, .kb-image43817_0076d0-d8 .kb-image-is-ratio-size{max-width:430px;width:100%;}.wp-block-kadence-column > .kt-inside-inner-col > .kb-image43817_0076d0-d8.kb-image-is-ratio-size, .wp-block-kadence-column > .kt-inside-inner-col > .kb-image43817_0076d0-d8 .kb-image-is-ratio-size{align-self:unset;}.kb-image43817_0076d0-d8 figure{max-width:430px;}.kb-image43817_0076d0-d8 .image-is-svg, .kb-image43817_0076d0-d8 .image-is-svg img{width:100%;}.kb-image43817_0076d0-d8 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43817_0076d0-d8\"><figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"900\" height=\"292\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-13.png\" alt=\"\" class=\"kb-img wp-image-23158\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-13.png 900w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-13-300x97.png 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-13-768x249.png 768w\" sizes=\"auto, (max-width: 900px) 100vw, 900px\" \/><\/figure><\/div>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 9:<\/strong>\u00a0Xanthine analysis can be performed in clinical settings, in sports to target misuse, or even in food analyses. A Force FluoroPhenyl column offers fast, simultaneous analysis of multiple compounds including isobars paraxanthine and theophylline.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0672.png\" alt=\"Xanthines on Force FluoroPhenyl by LC\/MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_CF0672<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Theobromine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/83-67-0\/Theobromine\" target=\"_blank\" rel=\"noopener\">Theobromine<\/a><\/td><td class=\"oth\">1.53<\/td><td class=\"oth\">50<\/td><td class=\"oth\">181.13<\/td><td class=\"oth\">137.95<\/td><td class=\"oth\">163.01<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Theophylline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/58-55-9\/Theophylline\" target=\"_blank\" rel=\"noopener\">Theophylline<\/a><\/td><td class=\"oth\">1.79<\/td><td class=\"oth\">50<\/td><td class=\"oth\">181.13<\/td><td class=\"oth\">124.02<\/td><td class=\"oth\">96.03<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Paraxanthine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/611-59-6\/Paraxanthine\" target=\"_blank\" rel=\"noopener\">Paraxanthine<\/a><\/td><td class=\"oth\">1.95<\/td><td class=\"oth\">50<\/td><td class=\"oth\">181.13<\/td><td class=\"oth\">124.02<\/td><td class=\"oth\">96.03<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Caffeine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/58-08-2\/Caffeine\" target=\"_blank\" rel=\"noopener\">Caffeine<\/a><\/td><td class=\"oth\">2.33<\/td><td class=\"oth\">50<\/td><td class=\"oth\">195.09<\/td><td class=\"oth\">138.01<\/td><td class=\"oth\">110.03<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Force FluoroPhenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9639212?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0672\" rel=\"noopener\">cat.# 9639212<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>1.8 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>70:30 Water:methanol<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>50 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.1% Formic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.3<\/td><td>70<\/td><td>30<\/td><\/tr><tr><td>2.5<\/td><td>0.3<\/td><td>30<\/td><td>70<\/td><\/tr><tr><td>3.0<\/td><td>0.3<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>3.01<\/td><td>0.3<\/td><td>70<\/td><td>30<\/td><\/tr><tr><td>5.0<\/td><td>0.3<\/td><td>70<\/td><td>30<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0672.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 10:<\/strong>\u00a0Restek&#8217;s Force FluoroPhenyl column makes quick and effective work of nitrofurans, which are often used in animal feed as antibiotics\/antimicrobials but are also banned in many regions.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0507.png\" alt=\"Nitrofurans on Force FluoroPhenyl by UHPLC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0507<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nitrofurantoin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/67-20-9\/Nitrofurantoin\" target=\"_blank\" rel=\"noopener\">Nitrofurantoin<\/a><\/td><td class=\"oth\">0.94<\/td><td class=\"oth\">239.1<\/td><td class=\"oth\">121.9<\/td><td class=\"oth\">95.0<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nitrofurazone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/59-87-0\/Nitrofurazone\" target=\"_blank\" rel=\"noopener\">Nitrofurazone<\/a><\/td><td class=\"oth\">0.99<\/td><td class=\"oth\">199.1<\/td><td class=\"oth\">107.9<\/td><td class=\"oth\">54.0<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Furazolidone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/67-45-8\/Furazolidone\" target=\"_blank\" rel=\"noopener\">Furazolidone<\/a><\/td><td class=\"oth\">1.12<\/td><td class=\"oth\">226.2<\/td><td class=\"oth\">95.0<\/td><td class=\"oth\">67.0<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nifuroxazide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/965-52-6\/Nifuroxazide\" target=\"_blank\" rel=\"noopener\">Nifuroxazide<\/a><\/td><td class=\"oth\">1.40<\/td><td class=\"oth\">276.2<\/td><td class=\"oth\">121.0<\/td><td class=\"oth\">93.0<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Force FluoroPhenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9639252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0507\" rel=\"noopener\">cat.# 9639252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>1.8 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>50 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.1% Acetic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>70<\/td><td>30<\/td><\/tr><tr><td>1.50<\/td><td>0.4<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>1.51<\/td><td>0.4<\/td><td>70<\/td><td>30<\/td><\/tr><tr><td>3.50<\/td><td>0.4<\/td><td>70<\/td><td>30<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0507.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 11:<\/strong>\u00a0You can pair a 1.8 \u03bcm Force column with an UltraShield UHPLC Pre\u00adColumn filter to prolong column lifetime\u2014without significantly altering retention times.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0569.png\" alt=\"Comparison of Isoflavones on Force C18 with and without UltraShield by UHPLC-MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FF0569<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 50px\">SIR (m\/z)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Daidzin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/552-66-9\/Daidzin\" target=\"_blank\" rel=\"noopener\">Daidzin<\/a><\/td><td class=\"oth\">417.2<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Genistin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/529-59-9\/Genistin\" target=\"_blank\" rel=\"noopener\">Genistin<\/a><\/td><td class=\"oth\">433.2<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Daidzein\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/468-66-8\/Daidzein\" target=\"_blank\" rel=\"noopener\">Daidzein<\/a><\/td><td class=\"oth\">255.1<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Genistein\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/446-72-0\/Genistein\" target=\"_blank\" rel=\"noopener\">Genistein<\/a><\/td><td class=\"oth\">271.1<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Force C18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9634252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FF0569\" rel=\"noopener\">cat.# 9634252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>1.8 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>UltraShield UHPLC precolumn filter 0.2 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/25810?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FF0569\" rel=\"noopener\">cat.# 25810<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>50 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Custom mix<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>500 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water + 0.1% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile + 0.1% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.6<\/td><td>85<\/td><td>15<\/td><\/tr><tr><td>1.50<\/td><td>0.6<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>1.51<\/td><td>0.6<\/td><td>85<\/td><td>15<\/td><\/tr><tr><td>3.00<\/td><td>0.6<\/td><td>85<\/td><td>15<\/td><\/tr><\/tbody><\/table><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Max Pressure:<\/th><td>500 bar<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Interface:<\/th><td>ESI+ <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0569.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-514ef67d wp-block-columns-is-layout-flex\" style=\"background-color:#e5f7fa;margin-bottom:0px\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"padding-top:20px;padding-bottom:20px\">\n<h2 class=\"wp-block-heading\">SPP or FPP?<\/h2>\n\n\n\n<p>Superficially porous particles (SPP) are becoming increasingly popular for providing faster, more efficient analyses without UHPLC pressures. And when speed is your goal, Restek recommends the Raptor line of\u00a0<a href=\"https:\/\/www.restek.com\/c\/1275\" target=\"_blank\" data-type=\"link\" data-id=\"https:\/\/www.restek.com\/c\/1275\" rel=\"noreferrer noopener\">LC columns<\/a>.<\/p>\n\n\n\n<p>However, retention is often just as important to sharpen peaks and increase sensitivity for mass spec, and when that is the case, fully porous particle (FPP) Force LC columns are ready to be put to work.<\/p>\n\n\n\n<p>Either way, Restek has a high-performing, reliable LC column for you.<\/p>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-a8e3f0c4 wp-block-columns-is-layout-flex\" style=\"background-color:#e5f7fa;margin-top:0px;margin-bottom:0px;padding-top:20px;padding-bottom:20px\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><style>.kb-image43817_d50c35-aa .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43817_d50c35-aa\"><figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"264\" height=\"264\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-14.png\" alt=\"\" class=\"kb-img wp-image-23168\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-14.png 264w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-14-150x150.png 150w\" sizes=\"auto, (max-width: 264px) 100vw, 264px\" \/><figcaption><strong>SPP<\/strong><\/figcaption><\/figure><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><style>.kb-image43817_f7a749-b5 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43817_f7a749-b5\"><figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"264\" height=\"264\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-15.png\" alt=\"\" class=\"kb-img wp-image-23174\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-15.png 264w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-15-150x150.png 150w\" sizes=\"auto, (max-width: 264px) 100vw, 264px\" \/><figcaption><strong>FPP<\/strong><\/figcaption><\/figure><\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-adfb9f59 wp-block-columns-is-layout-flex\" style=\"background-color:#e5f7fa;margin-top:0px;margin-bottom:0px\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"padding-top:20px;padding-bottom:20px\"><style>.kb-image43817_759f93-58 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43817_759f93-58\"><figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"900\" height=\"900\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-16.png\" alt=\"\" class=\"kb-img wp-image-23180\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-16.png 900w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-16-300x300.png 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-16-150x150.png 150w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNSS2598-16-768x768.png 768w\" sizes=\"auto, (max-width: 900px) 100vw, 900px\" \/><\/figure><\/div>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><style>.wp-block-kadence-advancedbtn.kb-btns43817_20d4e0-3d{gap:var(--global-kb-gap-xs, 0.5rem );justify-content:center;align-items:center;}.kt-btns43817_20d4e0-3d .kt-button{font-weight:normal;font-style:normal;}.kt-btns43817_20d4e0-3d .kt-btn-wrap-0{margin-right:5px;}.wp-block-kadence-advancedbtn.kt-btns43817_20d4e0-3d .kt-btn-wrap-0 .kt-button{color:#555555;border-color:#555555;}.wp-block-kadence-advancedbtn.kt-btns43817_20d4e0-3d .kt-btn-wrap-0 .kt-button:hover, .wp-block-kadence-advancedbtn.kt-btns43817_20d4e0-3d .kt-btn-wrap-0 .kt-button:focus{color:#ffffff;border-color:#444444;}.wp-block-kadence-advancedbtn.kt-btns43817_20d4e0-3d .kt-btn-wrap-0 .kt-button::before{display:none;}.wp-block-kadence-advancedbtn.kt-btns43817_20d4e0-3d .kt-btn-wrap-0 .kt-button:hover, .wp-block-kadence-advancedbtn.kt-btns43817_20d4e0-3d .kt-btn-wrap-0 .kt-button:focus{background:#444444;}<\/style>\n<div class=\"wp-block-kadence-advancedbtn kb-buttons-wrap kb-btns43817_20d4e0-3d\"><style>ul.menu .wp-block-kadence-advancedbtn .kb-btn43817_5430df-14.kb-button{width:initial;}.wp-block-kadence-advancedbtn .kb-btn43817_5430df-14.kb-button{background:#3278a9;}<\/style><a class=\"kb-button kt-button button kb-btn43817_5430df-14 kt-btn-size-standard kt-btn-width-type-full kb-btn-global-fill  kt-btn-has-text-true kt-btn-has-svg-false  wp-block-kadence-singlebtn\" href=\"https:\/\/www.restek.com\/c\/1760\" target=\"_blank\" rel=\"noreferrer noopener\"><span class=\"kt-btn-inner-text\"><strong>Browse Force<br>Products<\/strong><\/span><\/a><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><style>.wp-block-kadence-advancedbtn.kb-btns43817_52c94d-78{gap:var(--global-kb-gap-xs, 0.5rem );justify-content:center;align-items:center;}.kt-btns43817_52c94d-78 .kt-button{font-weight:normal;font-style:normal;}.kt-btns43817_52c94d-78 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kt-btn-size-standard kt-btn-width-type-full kb-btn-global-fill  kt-btn-has-text-true kt-btn-has-svg-false  wp-block-kadence-singlebtn\" href=\"https:\/\/discover.restek.com\/?s=force\"><span class=\"kt-btn-inner-text\"><strong><strong>Browse Force<br>Literature<\/strong><\/strong><\/span><\/a><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><style>.wp-block-kadence-advancedbtn.kb-btns43817_5cc4b9-d7{gap:var(--global-kb-gap-xs, 0.5rem );justify-content:center;align-items:center;}.kt-btns43817_5cc4b9-d7 .kt-button{font-weight:normal;font-style:normal;}.kt-btns43817_5cc4b9-d7 .kt-btn-wrap-0{margin-right:5px;}.wp-block-kadence-advancedbtn.kt-btns43817_5cc4b9-d7 .kt-btn-wrap-0 .kt-button{color:#555555;border-color:#555555;}.wp-block-kadence-advancedbtn.kt-btns43817_5cc4b9-d7 .kt-btn-wrap-0 .kt-button:hover, .wp-block-kadence-advancedbtn.kt-btns43817_5cc4b9-d7 .kt-btn-wrap-0 .kt-button:focus{color:#ffffff;border-color:#444444;}.wp-block-kadence-advancedbtn.kt-btns43817_5cc4b9-d7 .kt-btn-wrap-0 .kt-button::before{display:none;}.wp-block-kadence-advancedbtn.kt-btns43817_5cc4b9-d7 .kt-btn-wrap-0 .kt-button:hover, .wp-block-kadence-advancedbtn.kt-btns43817_5cc4b9-d7 .kt-btn-wrap-0 .kt-button:focus{background:#444444;}<\/style>\n<div class=\"wp-block-kadence-advancedbtn kb-buttons-wrap kb-btns43817_5cc4b9-d7\"><style>ul.menu .wp-block-kadence-advancedbtn .kb-btn43817_39dcf6-4e.kb-button{width:initial;}.wp-block-kadence-advancedbtn .kb-btn43817_39dcf6-4e.kb-button{background:#3278a9;}<\/style><a class=\"kb-button kt-button button kb-btn43817_39dcf6-4e kt-btn-size-standard kt-btn-width-type-full kb-btn-global-fill  kt-btn-has-text-true kt-btn-has-svg-false  wp-block-kadence-singlebtn\" href=\"https:\/\/www.restek.com\/chromatogram-library?s=force\" target=\"_blank\" rel=\"noreferrer noopener\"><span class=\"kt-btn-inner-text\"><strong><strong>Browse Force<br>Chromatograms<\/strong><\/strong><\/span><\/a><\/div>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\" style=\"background-color:#e5f7fa\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><style>.kb-image43817_98a5cb-47.kb-image-is-ratio-size, .kb-image43817_98a5cb-47 .kb-image-is-ratio-size{max-width:346px;width:100%;}.wp-block-kadence-column > .kt-inside-inner-col > .kb-image43817_98a5cb-47.kb-image-is-ratio-size, .wp-block-kadence-column > .kt-inside-inner-col > .kb-image43817_98a5cb-47 .kb-image-is-ratio-size{align-self:unset;}.kb-image43817_98a5cb-47 figure{max-width:346px;}.kb-image43817_98a5cb-47 .image-is-svg, .kb-image43817_98a5cb-47 .image-is-svg img{width:100%;}.kb-image43817_98a5cb-47 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43817_98a5cb-47\"><figure class=\"alignright size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"400\" height=\"123\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/teaser-product-lc-columns-inert-lc-02.png\" alt=\"\" class=\"kb-img wp-image-29768\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/teaser-product-lc-columns-inert-lc-02.png 400w, https:\/\/discover.restek.com\/wp-content\/uploads\/teaser-product-lc-columns-inert-lc-02-300x92.png 300w\" sizes=\"auto, (max-width: 400px) 100vw, 400px\" \/><\/figure><\/div>\n\n\n\n<h4 class=\"wp-block-heading\">Want even better performance when analyzing mycotoxins and other metal-sensitive compounds?<\/h4>\n\n\n\n<p>Learn more at\u00a0<a href=\"https:\/\/discover.restek.com\/articles\/gnss4155\/accurately-analyze-metal-sensitive-compounds-with-resteks-new-inert-lc-columns\" data-type=\"link\" data-id=\"https:\/\/discover.restek.com\/articles\/gnss4155\/accurately-analyze-metal-sensitive-compounds-with-resteks-new-inert-lc-columns\">www.restek.com\/inert<\/a><\/p>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Rugged and reliable, Force LC columns are fully scalable between 3 or 5 \u00b5m HPLC and 1.8 \u00b5m UHPLC, so you can easily transfer and optimize methods.<\/p>\n","protected":false},"author":11,"featured_media":7387,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[7],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[2391,2373],"resource-technique":[2299],"hf_cat_post":[650],"ppma_author":[414],"class_list":["post-43817","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles","product-library-lc-columns","product-library-liquid-chromatography-products","resource-technique-liquid-chromatography"],"acf":[],"taxonomy_info":{"category":[{"value":7,"label":"Articles"}],"product-library":[{"value":2391,"label":"LC 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