{"id":60239,"date":"2025-11-05T16:58:36","date_gmt":"2025-11-05T16:58:36","guid":{"rendered":"https:\/\/discover.restek.com\/?p=60239"},"modified":"2026-04-14T17:47:03","modified_gmt":"2026-04-14T17:47:03","slug":"simplifying-epa-1633-analyses-with-an-improved-dual-bed-solid-phase-extraction-method","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/es\/posters-presentations\/gnot5277\/simplifying-epa-1633-analyses-with-an-improved-dual-bed-solid-phase-extraction-method","title":{"rendered":"Simplifying EPA 1633 Analyses with an Improved Dual-Bed Solid-Phase Extraction Method"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">Abstract: Solid-phase extraction (SPE) is a valuable sample preparation technique for environmental matrices utilized in extracting pesticides, semivolatiles, and emerging contaminants from water samples. Despite its efficacy, SPE encounters challenges in non-potable water matrices where traditional ion-exchange SPE fails to remove certain matrix interferences, leading to the implementation of dispersive carbon protocols. Furthermore, solid particles in the sample can obstruct SPE cartridges, prolonging extraction or needing multiple cartridges to complete the analysis. The recent EPA Method 1633 aims at the analyses of PFAS in aqueous, solids, biosolids, and tissue samples where extract cleanup steps, as well as percent solids, can affect the overall length and performance of the sample preparation. In this work, WAX and Graphitized Carbon Black (GCB) dual-bed SPE cartridges were compared to conventional single-bed SPE formats towards precision, accuracy, and reduction of semi and nonvolatile residues. Furthermore, a novel filter aid was assessed for the mitigation of cartridge clogging and compared to glass wool using an ASTM substitute wastewater matrix. The recoveries for all 40 PFAS analytes in the fortified sample ranged from 80%-120%, and the relative standard deviation was less than 20% for most of the analytes. The advantage of the filter aid was significant as it provided 60% better filtration efficiency when compared to glass wool and over 90% when compared to the unfiltered SPE controls. The experimental SPE format offered a simplified and consistent approach when dealing with dirty matrices while meeting the accuracy and precision of EPA 1633. Details on the HPLC parameters afforded will also be discussed.<\/p>\n\n\n\n<div data-wp-interactive=\"core\/file\" class=\"wp-block-file\"><object data-wp-bind--hidden=\"!state.hasPdfPreview\" hidden class=\"wp-block-file__embed\" data=\"https:\/\/discover.restek.com\/wp-content\/uploads\/EPA1633.pdf\" type=\"application\/pdf\" style=\"width:100%;height:600px\" aria-label=\"Embed of EPA1633.\"><\/object><a id=\"wp-block-file--media-82cb8d70-8944-4119-b45f-2b1ef0a887eb\" href=\"https:\/\/discover.restek.com\/wp-content\/uploads\/EPA1633.pdf\">EPA1633<\/a><a href=\"https:\/\/discover.restek.com\/wp-content\/uploads\/EPA1633.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-82cb8d70-8944-4119-b45f-2b1ef0a887eb\">Download<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Abstract: Solid-phase extraction (SPE) is a valuable sample preparation technique for environmental matrices utilized in extracting pesticides, semivolatiles, and emerging contaminants from water samples. Despite its efficacy, SPE encounters challenges in non-potable water matrices where traditional ion-exchange SPE fails to remove certain matrix interferences, leading to the implementation of dispersive carbon protocols. Furthermore, solid particles in the sample can obstruct SPE cartridges, prolonging extraction or needing multiple cartridges to complete the analysis. The recent EPA Method 1633 aims at the analyses of PFAS in aqueous, solids, biosolids, and tissue samples where extract cleanup steps, as well as percent solids, can affect the overall length and performance of the sample preparation. In this work, WAX and Graphitized Carbon Black (GCB) dual-bed SPE cartridges were compared to conventional single-bed SPE formats towards precision, accuracy, and reduction of semi and nonvolatile residues. Furthermore, a novel filter aid was assessed for the mitigation of cartridge clogging and compared to glass wool using an ASTM substitute wastewater matrix. The recoveries for all 40 PFAS analytes in the fortified sample ranged from 80%-120%, and the relative standard deviation was less than 20% for most of the analytes. The advantage of the filter aid was significant as it provided 60% better filtration efficiency when compared to glass wool and over 90% when compared to the unfiltered SPE controls. The experimental SPE format offered a simplified and consistent approach when dealing with dirty matrices while meeting the accuracy and precision of EPA 1633. Details on the HPLC parameters afforded will also be discussed.<\/p>\n","protected":false},"author":31,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[12],"tags":[2782],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[],"ppma_author":[451,447,456,458,452],"class_list":["post-60239","post","type-post","status-publish","format-standard","hentry","category-posters-presentations","tag-pfas"],"acf":[],"taxonomy_info":{"category":[{"value":12,"label":"Posters &amp; Presentations"}],"post_tag":[{"value":2782,"label":"PFAS"}]},"featured_image_src_large":false,"author_info":{"display_name":"Diego L\u00f3pez","author_link":"https:\/\/discover.restek.com\/es\/author\/diego-lopez\/"},"comment_info":0,"category_info":[{"term_id":12,"name":"Posters &amp; Presentations","slug":"posters-presentations","term_group":0,"term_taxonomy_id":12,"taxonomy":"category","description":"","parent":0,"count":118,"filter":"raw","cat_ID":12,"category_count":118,"category_description":"","cat_name":"Posters &amp; Presentations","category_nicename":"posters-presentations","category_parent":0}],"tag_info":[{"term_id":2782,"name":"PFAS","slug":"pfas","term_group":0,"term_taxonomy_id":2782,"taxonomy":"post_tag","description":"","parent":0,"count":113,"filter":"raw"}],"authors":[{"term_id":451,"user_id":31,"is_guest":0,"slug":"diego-lopez","display_name":"Diego L\u00f3pez","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-lopez-diego.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-lopez-diego.jpg"},"author_category":"1","first_name":"Diego","last_name":"L\u00f3pez","user_url":"","job_title":"","description":"Diego is the product manager for sample preparation at Restek Corporation. He has over 10 years of experience in liquid chromatography for both chiral and achiral separations and holds a PhD in chemistry from The University of Texas at Arlington."},{"term_id":447,"user_id":27,"is_guest":0,"slug":"jason-hoisington","display_name":"Jason Hoisington","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-hoisington-jason-01.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-hoisington-jason-01.jpg"},"author_category":"1","first_name":"Jason","last_name":"Hoisington","user_url":"","job_title":"","description":"Jason Hoisington received his bachelor\u2019s degree in general science with a focus on chemistry from the University of Alaska, Fairbanks. He worked for SGS Environmental for seven years in environmental soil and water testing, developing methods for the analysis of volatiles and semivolatile organics to include pesticides and polychlorinated biphenyls (PCBs). In 2012, Jason moved on to lab and application support for Dow Chemical Company, providing advanced analytical troubleshooting and method development. In 2019, Jason joined Restek and has focused on air applications."},{"term_id":456,"user_id":36,"is_guest":0,"slug":"alexis-shelow","display_name":"Alexis Shelow","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-shelow-alexis.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-shelow-alexis.jpg"},"author_category":"1","first_name":"Alexis","last_name":"Shelow","user_url":"https:\/\/www.linkedin.com\/in\/alexis-shelow-531b4513b\/","job_title":"SP Solutions Scientist (R&D)","description":"Alexis is a scientist in the sample preparation R&amp;D department. Her primary focus is SPE in the environmental sector. In her previous role at Restek as an LC manufacturing chemist, she developed skills related to synthesizing silica particles, bonding stationary phases, and optimizing procedural workflows. Alexis attended Delaware Valley University where she earned her BS in wildlife biology."},{"term_id":458,"user_id":38,"is_guest":0,"slug":"jason-thomas","display_name":"Jason Thomas","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-thomas-jason.png","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-thomas-jason.png"},"author_category":"1","first_name":"Jason","last_name":"Thomas","user_url":"","job_title":"","description":"Jason first came to \"Chromatography Valley,\" the location of Restek's state-of-the-art manufacturing and development facility, as a student of chemistry at The Pennsylvania State University. Upon graduation, he ventured into the environmental and chemical industries for four years before finding a home at Restek, where he has been involved in GC column development, environmental applications, and SPE product development since 2002."},{"term_id":452,"user_id":32,"is_guest":0,"slug":"colton-myers","display_name":"Colton Myers","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-myers-colton.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-myers-colton.jpg"},"author_category":"1","first_name":"Colton","last_name":"Myers","user_url":"https:\/\/www.linkedin.com\/in\/myerscolton\/","job_title":"Product Manager - New Product Development","description":"Colton Myers is a Product Manager for GC columns at Restek Corporation with over 10 years of experience in research and development. He specializes in driving new GC column technologies from concept to market, combining deep technical expertise with a strong focus on innovation and commercialization. Colton holds a BS in chemistry from Juniata College and a Master of Management in Corporate Innovation and Entrepreneurship from Penn State University."}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/60239","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/users\/31"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/comments?post=60239"}],"version-history":[{"count":1,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/60239\/revisions"}],"predecessor-version":[{"id":60241,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/60239\/revisions\/60241"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/media?parent=60239"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/categories?post=60239"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/tags?post=60239"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/industries-application?post=60239"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/post-badge?post=60239"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/resource-type?post=60239"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/product-library?post=60239"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/resource-technique?post=60239"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/ppma_author?post=60239"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}