{"id":60509,"date":"2025-11-07T14:04:49","date_gmt":"2025-11-07T14:04:49","guid":{"rendered":"https:\/\/discover.restek.com\/?p=60509"},"modified":"2026-01-08T14:21:45","modified_gmt":"2026-01-08T14:21:45","slug":"the-benefits-of-2-1-mm-internal-diameter-analytical-columns-for-the-analysis-of-drugs-of-abuse-by-lc-ms-ms","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/es\/posters-presentations\/gnot5308\/the-benefits-of-2-1-mm-internal-diameter-analytical-columns-for-the-analysis-of-drugs-of-abuse-by-lc-ms-ms","title":{"rendered":"The Benefits of 2.1 mm Internal Diameter Analytical Columns for the Analysis of Drugs of Abuse by LC-MS\/MS"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">The biphenyl stationary phase offers advantageous selectivity compared to a C18 column for drugs of abuse panels, but choosing the right column dimension is key to obtaining robust and accurate data. Every column dimension can be advantageous in different scenarios, but generally clinical labs are all working towards the same goals: high throughput, low sample volume, good sensitivity, and low cost. In this work, the advantage of 2.1 mm internal diameter (ID) columns is discussed and demonstrated for the analysis of drugs of abuse by LC-MS\/MS.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Two methods were developed to analyze common isobars in drugs of abuse panels on Raptor Biphenyl columns: one method used a 50 x 2.1 mm, 2.7 \u03bcm column with a flow rate of 0.6 mL\/min and the other used a 50 x 4.6 mm, 2.7 \u03bcm column with a flow rate of 0.9 mL\/min. The two methods were compared for efficiency, sensitivity, resolution (Rs), consumption of mobile phase, and robustness. Buprenorphine was used to demonstrate sensitivity differences between the two different column dimensions. It was found that the 2.1 mm ID column produced twice the signal response of the 4.6mm internal diameter column when injection volume is held constant. Twice the amount of sample had to be injected on the larger-bore column to produce the same signal response, thus introducing the chromatography to greater matrix effects with more impact to instrument cleanliness.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">In summary, the 2.1 mm ID column was able to demonstrate superior sensitivity while consuming less resources and minimizing impact to the MS while still providing adequate resolution of isobars and excellent column robustness.<\/p>\n\n\n\n<div data-wp-interactive=\"core\/file\" class=\"wp-block-file\"><object data-wp-bind--hidden=\"!state.hasPdfPreview\" hidden class=\"wp-block-file__embed\" data=\"https:\/\/discover.restek.com\/wp-content\/uploads\/poster-msacl-2024-Benefits-of-2.1-mm-Internal-Diameter-Analytical-Columns-for-the-Analysis-of-Drugs-of-Abuse.pdf\" type=\"application\/pdf\" style=\"width:100%;height:600px\" aria-label=\"Embed of poster-msacl-2024-Benefits of 2.1 mm Internal Diameter Analytical Columns for the Analysis of Drugs of Abuse.\"><\/object><a id=\"wp-block-file--media-d8586ba0-e7a0-4110-94be-3de33d5576b5\" href=\"https:\/\/discover.restek.com\/wp-content\/uploads\/poster-msacl-2024-Benefits-of-2.1-mm-Internal-Diameter-Analytical-Columns-for-the-Analysis-of-Drugs-of-Abuse.pdf\">poster-msacl-2024-Benefits of 2.1 mm Internal Diameter Analytical Columns for the Analysis of Drugs of Abuse<\/a><a href=\"https:\/\/discover.restek.com\/wp-content\/uploads\/poster-msacl-2024-Benefits-of-2.1-mm-Internal-Diameter-Analytical-Columns-for-the-Analysis-of-Drugs-of-Abuse.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-d8586ba0-e7a0-4110-94be-3de33d5576b5\">Download<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"<p>The biphenyl stationary phase offers advantageous selectivity compared to a C18 column for drugs of abuse panels, but choosing the right column dimension is key to obtaining robust and accurate data. Every column dimension can be advantageous in different scenarios, but generally clinical labs are all working towards the same goals: high throughput, low sample volume, good sensitivity, and low cost. In this work, the advantage of 2.1 mm internal diameter (ID) columns is discussed and demonstrated for the analysis of drugs of abuse by LC-MS\/MS. <\/p>\n<p>Two methods were developed to analyze common isobars in drugs of abuse panels on Raptor Biphenyl columns: one method used a 50 x 2.1 mm, 2.7 \u03bcm column with a flow rate of 0.6 mL\/min and the other used a 50 x 4.6 mm, 2.7 \u03bcm column with a flow rate of 0.9 mL\/min. The two methods were compared for efficiency, sensitivity, resolution (Rs), consumption of mobile phase, and robustness. Buprenorphine was used to demonstrate sensitivity differences between the two different column dimensions. It was found that the 2.1 mm ID column produced twice the signal response of the 4.6mm internal diameter column when injection volume is held constant. Twice the amount of sample had to be injected on the larger-bore column to produce the same signal response, thus introducing the chromatography to greater matrix effects with more impact to instrument cleanliness. <\/p>\n<p>In summary, the 2.1 mm ID column was able to demonstrate superior sensitivity while consuming less resources and minimizing impact to the MS while still providing adequate resolution of isobars and excellent column robustness.<\/p>\n","protected":false},"author":24,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[12],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[],"ppma_author":[444,579],"class_list":["post-60509","post","type-post","status-publish","format-standard","hentry","category-posters-presentations"],"acf":[],"taxonomy_info":{"category":[{"value":12,"label":"Posters &amp; Presentations"}]},"featured_image_src_large":false,"author_info":{"display_name":"Samantha Herbick","author_link":"https:\/\/discover.restek.com\/es\/author\/samantha-herbick\/"},"comment_info":0,"category_info":[{"term_id":12,"name":"Posters &amp; Presentations","slug":"posters-presentations","term_group":0,"term_taxonomy_id":12,"taxonomy":"category","description":"","parent":0,"count":118,"filter":"raw","cat_ID":12,"category_count":118,"category_description":"","cat_name":"Posters &amp; Presentations","category_nicename":"posters-presentations","category_parent":0}],"tag_info":false,"authors":[{"term_id":444,"user_id":24,"is_guest":0,"slug":"samantha-herbick","display_name":"Samantha Herbick","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-herbick-samantha.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-herbick-samantha.jpg"},"author_category":"1","first_name":"Samantha","last_name":"Herbick","user_url":"","job_title":"","description":"Samantha Herbick is an applications scientist within the LC Solutions Department at Restek. Her primary focus is on the development of applications in the toxicology and life science markets. She attended Duquesne University where she earned a bachelor\u2019s degree in Biochemistry and a master's degree in forensic science and law. Prior to joining Restek, Samantha worked as a scientist and method developer in a forensic toxicology lab. In this role, she performed analysis on toxicology casework and worked on the development and validation of new and existing assays using LC-MS\/MS, GC-MS, and GC-FID."},{"term_id":579,"user_id":47,"is_guest":0,"slug":"jamie-l-york-phd","display_name":"Jamie L. York, PhD","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/york-jamie-1000x1000-1-e1765483005988.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/york-jamie-1000x1000-1-e1765483005988.jpg"},"author_category":"1","first_name":"Jamie","last_name":"York","user_url":"","job_title":"","description":"Jamie York is a principal scientist in the Applications Lab at Restek Corporation. She leads the development of innovative analytical methods tailored to the food, clinical, environmental, and cannabis markets. Jamie earned her PhD in chemistry from The University of Texas at Arlington, where she gained extensive expertise in a range of analytical techniques, including gas chromatography\u2013vacuum ultraviolet (GC\u2013VUV); gas chromatography\u2013mass spectrometry (GC\u2013MS); matrix-assisted laser desorption\/ionization (MALDI); and liquid chromatography\u2013 mass spectrometry (LC\u2013MS\/MS); with a research emphasis on food and environmental analysis. Today, her work focuses on complex method development and advanced sample preparation strategies to support the evolving needs of the scientific community."}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/60509","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/users\/24"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/comments?post=60509"}],"version-history":[{"count":2,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/60509\/revisions"}],"predecessor-version":[{"id":60732,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/posts\/60509\/revisions\/60732"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/media?parent=60509"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/categories?post=60509"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/tags?post=60509"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/industries-application?post=60509"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/post-badge?post=60509"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/resource-type?post=60509"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/product-library?post=60509"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/resource-technique?post=60509"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/es\/wp-json\/wp\/v2\/ppma_author?post=60509"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}