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BEGIN:DAYLIGHT
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TZOFFSETTO:-0300
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DTSTART:20250309T060000
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DTSTART:20251102T050000
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DTSTART:20260308T060000
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DTSTART:20270314T060000
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TZOFFSETFROM:-0300
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DTSTART:20271107T050000
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BEGIN:STANDARD
TZOFFSETFROM:+0900
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BEGIN:VEVENT
DTSTART;VALUE=DATE:20260608
DTEND;VALUE=DATE:20260613
DTSTAMP:20260522T091321Z
CREATED:20260324T123001Z
LAST-MODIFIED:20260522T091321Z
UID:10000045-1780876800-1781308799@discover.restek.com
SUMMARY:EPRW (European Pesticide Residue Workshop) 2026
DESCRIPTION:Join us for an exciting EPRW 2026 in Rotterdam\, The Netherlands! \nGet a sneak peek of what we’re going to present→
URL:https://discover.restek.com/event/eprw-european-pesticide-residue-workshop-2026/
LOCATION:Postillion Hotel & Convention Centre WTC\, Beursplein 37\, Rotterdam\, Netherlands
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20260616
DTEND;VALUE=DATE:20260618
DTSTAMP:20260324T123452Z
CREATED:20260324T123452Z
LAST-MODIFIED:20260324T123452Z
UID:10000046-1781568000-1781740799@discover.restek.com
SUMMARY:Lab Italia 2026
DESCRIPTION:
URL:https://discover.restek.com/event/lab-italia-2026/
LOCATION:NH Milano Congress Centre\, Strada 2a\, Milano\, 20057\, Italy
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20260706
DTEND;VALUE=DATE:20260710
DTSTAMP:20260323T204033Z
CREATED:20260323T204033Z
LAST-MODIFIED:20260323T204033Z
UID:10000043-1783296000-1783641599@discover.restek.com
SUMMARY:Extech 2026
DESCRIPTION:
URL:https://discover.restek.com/event/extech-2026/
LOCATION:Gembloux Agro Bio Tech\, Espace Senghor\, Avenue de la faculté d'agronomie 11\, Gembloux\, 5030\, Belgium
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=UTC:20260707T110000
DTEND;TZID=UTC:20260707T120000
DTSTAMP:20260701T235829Z
CREATED:20260629T141441Z
LAST-MODIFIED:20260701T235829Z
UID:10000339-1783422000-1783425600@discover.restek.com
SUMMARY:Live Webinar: From Constraint to Opportunity: Optimizing GC Methods Post–Methylene Chloride Ban
DESCRIPTION:Join us for this webinar to learn about the impact of the methylene chloride ban on EPA 8270 semivolatile methods and how how innovative GC column technology\, paired with high-quality reference standards and optimized GC-MS/MS methods\, can restore and exceed required sensitivity
URL:https://discover.restek.com/event/live-webinar-from-constraint-to-opportunity-optimizing-gc-methods-post-methylene-chloride-ban/
LOCATION:https://globalmeet.webcasts.com/starthere.jsp?ei=1766442&tp_key=6381e0e721&sti=restek
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20260712
DTEND;VALUE=DATE:20260716
DTSTAMP:20260323T193914Z
CREATED:20260323T193852Z
LAST-MODIFIED:20260323T193914Z
UID:10000035-1783814400-1784159999@discover.restek.com
SUMMARY:NACRW 2026
DESCRIPTION:
URL:https://discover.restek.com/event/nacrw-2026/
LOCATION:Grand Hyatt San Antonio River Walk\, 600 E Market St\, San Antonio\, TX\, 78205\, United States
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20260721
DTEND;VALUE=DATE:20260725
DTSTAMP:20260421T175124Z
CREATED:20260421T175124Z
LAST-MODIFIED:20260421T175124Z
UID:10000286-1784592000-1784937599@discover.restek.com
SUMMARY:CAI 2026
DESCRIPTION:Salamanca\, Spain
URL:https://discover.restek.com/event/cai-2026/
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20260726
DTEND;VALUE=DATE:20260731
DTSTAMP:20260324T124223Z
CREATED:20260324T124223Z
LAST-MODIFIED:20260324T124223Z
UID:10000047-1785024000-1785455999@discover.restek.com
SUMMARY:ADLM (Association for Diagnostics & Laboratory Medicine) 2026
DESCRIPTION:
URL:https://discover.restek.com/event/adlm-association-for-diagnostics-laboratory-medicine-2026/
LOCATION:Anaheim Convention Center\, 800 W Katella Ave\, Anaheim\, CA\, 92802\, United States
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20260803
DTEND;VALUE=DATE:20260808
DTSTAMP:20260420T131052Z
CREATED:20260324T124721Z
LAST-MODIFIED:20260420T131052Z
UID:10000048-1785715200-1786147199@discover.restek.com
SUMMARY:NEMC/EMS (Environmental Measurement Symposium) 2026
DESCRIPTION:
URL:https://discover.restek.com/event/nemc-ems-environmental-measurement-symposium-2026/
LOCATION:Hyatt Regency Houston\, 1200 Louisiana Street\, Houston\, TX\, 77002\, United States
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20260822
DTEND;VALUE=DATE:20260829
DTSTAMP:20260728T131005Z
CREATED:20260325T124813Z
LAST-MODIFIED:20260728T131005Z
UID:10000049-1787356800-1787961599@discover.restek.com
SUMMARY:IMSC (International Mass Spectrometry Conference) 2026
DESCRIPTION:Join us for an exciting IMSC 2026 in Lyon\, France! \nGet a sneak peek of what we’re going to present→
URL:https://discover.restek.com/event/imsc-international-mass-spectrometry-conference-2026/
LOCATION:Centre de Congrès de Lyon\, 50 Quai Charles de Gaulle\, Lyon\, 69006\, France
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20260823
DTEND;VALUE=DATE:20260828
DTSTAMP:20260325T130053Z
CREATED:20260325T130053Z
LAST-MODIFIED:20260325T130053Z
UID:10000050-1787443200-1787875199@discover.restek.com
SUMMARY:ACS (American Chemical Society) Fall 2026
DESCRIPTION:
URL:https://discover.restek.com/event/acs-american-chemical-society-fall-2026/
LOCATION:McCormick Place Convention Center\, 2301 S. Dr. Martin Luther King Jr. (MLK) Drive\, Chicago\, IL\, 60616\, United States
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20260823
DTEND;VALUE=DATE:20260829
DTSTAMP:20260401T171915Z
CREATED:20260401T171915Z
LAST-MODIFIED:20260401T171915Z
UID:10000051-1787443200-1787961599@discover.restek.com
SUMMARY:MAFS (Midwestern Association of Forensic Scientists) 2026
DESCRIPTION:
URL:https://discover.restek.com/event/mafs-midwestern-association-of-forensic-scientists-2026/
LOCATION:Minneapolis Marriott City Center\, 30 South 7th Street\, Minneapolis\, MN\, 55402\, United States
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20260829
DTEND;VALUE=DATE:20260903
DTSTAMP:20260814T123811Z
CREATED:20260323T194158Z
LAST-MODIFIED:20260814T123811Z
UID:10000036-1787961600-1788393599@discover.restek.com
SUMMARY:AOAC 140th Annual Meeting 2026
DESCRIPTION:We’re going to AOAC 2026 in Indianapolis\, IN! Stop by Booth 220 to talk with our team about our next-gen RMX columns. Engineered to provide sharper peaks and longer lifetimes\, these new GC columns are ideal for food and enviro analyses. \nRestek’s Presentations at AOAC 2026\nVendor Seminar\nCutting Downtime\, Not Columns: Performance of a Next-Generation Inert Capillary Column for Multiresidue GC-MS/MS Analysis\nTuesday\, September 1\, 1:30–2:00 p.m. | Grand Ballroom 8\nPresenter: Jonathan DeCenzi & Ramkumar Dhandapani \nThe analysis of pesticide residues in complex botanical matrices is often complicated by high levels of coextracted starches\, oils\, water\, and other naturally occurring compounds that persist even after extensive sample cleanup. Achieving reliable detection and quantitation of pesticides at trace levels demands a chromatographic column with exceptional inertness\, resolution\, and long-term stability. We present the evaluation of a novel GC capillary column\, engineered for outstanding inertness and robustness in GC-MS/MS multiresidue pesticide analysis. Compared to conventional columns\, this new design delivers markedly improved peak shape\, signal-to-noise\, and resolution across a wide variety of challenging botanical matrices\, including ashwagandha root\, lavender oil\, and ginseng powder. A standout feature is its integrated fused silica retention gap at the inlet and fused silica transfer line at the outlet. This innovative construction ensures that retention times remain consistent\, even after trimming\, allowing for fast and reliable maintenance with no impact on chromatographic performance. \nTechnical Posters\nImproved Baseline Stability and Sensitivity in GC–MS Using a Novel Column with an Uncoated Interface Section\nMonday\, August 31\, all-day | Board #18\nPresenter: Ramkumar Dhandapani \nA novel gas chromatography–mass spectrometry (GC–MS) column design has been developed to enhance analytical performance by addressing persistent challenges in baseline stability and sensitivity. This innovation integrates a unique interface configuration that minimizes stationary phase degradation and thermal stress effects\, particularly under elevated transfer line temperatures. Comparative evaluations using a diverse panel of pesticide analytes demonstrated significantly faster baseline stabilization and reduced background noise relative to conventional columns. \nThe new column architecture consistently delivered improved signal-to-noise ratios across full-scan and SIM modes\, with pronounced benefits observed for high-boiling-point and late-eluting compounds. These enhancements suggest a substantial reduction in analyte loss and column bleed\, enabling more reliable detection of thermally labile substances. \nChallenges in Enhancing GC-MS/MS Analysis of Pesticides in Fruits\, Vegetables\, Cereals\, and Pulses in a High-Throughput Routine Laboratory\nMonday\, August 31\, all-day | Board #19\nPresenter: Ramkumar Dhandapani \nGC-MS/MS analysis on a conventional GC QQQ system is primarily governed by three factors: the choice of analytical separation column; the type of liner used; and the measurement approach—whether samples are analyzed in solvent or matrix\, and whether an analytical protectant (AP) is applied\, and which type. While deactivated liners with turbulence grooves are standard\, their influence—along with septa and regeneration—remains significant. Column choice is equally crucial\, excessive bleeding compromises sensitivity and peak resolution. Currently\, we use a traditional polyphenylmethylsiloxane phase with 5% phenyl and 95% dimethyl\, specifically optimized for mass spectrometry applications. While these columns deliver acceptable performance\, trials clearly demonstrate that more inert alternatives offer significantly greater stability and reliability\, such as RMX-5Sil MS with integra-transfer line. The third key factor is the measurement approach. Validation studies consistently show that the best results occur when standards and samples share the same matrix\, thereby compensating for matrix effects. In a high-throughput setting\, however\, preparing individual matrix standards for all samples is impractical. Currently\, we employ an analytical protectant recommended in the literature\, consisting primarily of a sugar mixture. This AP mix mitigates the otherwise observed overestimation of standards in solvent compared to matrix-containing samples. This strategy appears to offer the greatest potential for improving and stabilizing results. The requirements for an effective AP or matrix standard are stringent: results must remain stable across the entire liner and all matrix groups\, including fresh fruits and vegetables\, juices and concentrates\, rice\, cereals\, legumes\, dried fruits\, candied fruits\, and sugars. \nFurthermore\, the chosen matrix or AP mix must be reproducibly manufacturable and consistently available. Extensive trials suggest that sugars\, acids\, and starches are key components for an effective AP mix. However\, standardized mixtures of sugars\, ascorbic acid\, and various starches have not performed as well as using actual matrix\, indicating that secondary plant metabolites also play a role. Currently\, comparative measurements on selected pesticides using various test mixes are underway. Once the optimal matrix is identified\, full-scope validation will follow. \nImproving Method Robustness and Laboratory Throughput in GC-MS/MS Multiresidue Pesticide Analysis of Complex Botanical Matrices\nMonday\, August 31\, all-day | Board #15\nPresenter: Chanisse Rickard (NOW Foods) \nThe analysis of pesticide residues in complex botanical matrices is often complicated by high levels of coextracted starches\, oils\, water\, and other naturally occurring compounds that persist even after extensive sample cleanup. Achieving reliable detection and quantitation of pesticides at trace levels demands a chromatographic column with exceptional inertness\, resolution\, and long-term stability. We present the evaluation of a novel GC capillary column\, engineered for outstanding inertness and robustness in GC-MS/MS multiresidue pesticide analysis. Compared to conventional columns\, this new design delivers markedly improved peak shape\, signal-to-noise\, and resolution across a wide variety of challenging botanical matrices\, including ashwagandha root\, lavender oil\, and ginseng powder. A standout feature is its integrated fused silica retention gap at the inlet and fused silica transfer line at the outlet. This innovative construction ensures that retention times remain consistent\, even after trimming\, allowing for fast and reliable maintenance with no impact on chromatographic performance. \nOur Featured Solutions\n\nRMX GC Columns\n\nEnter Our Raffle!\nEnter our AOAC raffle for a chance to win an Apple iPad (128 GB)! \n 
URL:https://discover.restek.com/event/aoac-140th-annual-meeting-2026/
LOCATION:JW Marriott Indianapolis\, 10 S West St\, Indianapolis\, IN\, 46204\, United States
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20260902
DTEND;VALUE=DATE:20260905
DTSTAMP:20260323T202542Z
CREATED:20260323T202542Z
LAST-MODIFIED:20260323T202542Z
UID:10000042-1788307200-1788566399@discover.restek.com
SUMMARY:JASIS 2026
DESCRIPTION:
URL:https://discover.restek.com/event/jasis-2026/
LOCATION:Makuhari Messe International Exhibition Halls and International Conference Hall\, 2 Chome-1 Nakase\, Chiba\, 261-0023\, Japan
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20260906
DTEND;VALUE=DATE:20260910
DTSTAMP:20260422T131311Z
CREATED:20260422T131311Z
LAST-MODIFIED:20260422T131311Z
UID:10000298-1788652800-1788998399@discover.restek.com
SUMMARY:ISEO 2026 - 56th International Symposium on Essentials Oils
DESCRIPTION:
URL:https://discover.restek.com/event/iseo-2026-56th-international-symposium-on-essentials-oils/
LOCATION:Espace chiris\, Av. de Provence\, Grasse\, France
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20260906
DTEND;VALUE=DATE:20260911
DTSTAMP:20260812T115658Z
CREATED:20260401T210031Z
LAST-MODIFIED:20260812T115658Z
UID:10000052-1788652800-1789084799@discover.restek.com
SUMMARY:ISC (International Symposium on Chromatography) 2026
DESCRIPTION:Join us for an exciting ISC 2026 in Prague\, Czechia! \nGet a sneak peek of what we’re going to present→
URL:https://discover.restek.com/event/isc-international-symposium-on-chromatography-2026/
LOCATION:Prague Congress Centre\, 5.kvetna 65\, Prague\, Czech Republic
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20260913
DTEND;VALUE=DATE:20260919
DTSTAMP:20260901T085023Z
CREATED:20260403T140755Z
LAST-MODIFIED:20260901T085023Z
UID:10000053-1789257600-1789775999@discover.restek.com
SUMMARY:Dioxin 2026
DESCRIPTION:Join us for an exciting Dioxin 2026 in Riva Del Garda (Italy)! \nGet a sneak peek of what we’re going to present→
URL:https://discover.restek.com/event/dioxin-2026/
LOCATION:Riva Del Garda Congress Center\, Parco Lido\, Riva del Garda\, Italy
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20260914
DTEND;VALUE=DATE:20260917
DTSTAMP:20260403T141533Z
CREATED:20260403T141533Z
LAST-MODIFIED:20260403T141533Z
UID:10000054-1789344000-1789603199@discover.restek.com
SUMMARY:Deutsche Lebensmittelchemietage GDCh 2026
DESCRIPTION:
URL:https://discover.restek.com/event/deutsche-lebensmittelchemietage-gdch-2026/
LOCATION:Rhineland-Palatinate University of Technology Kaiserlautern-Landau\, Erwin-Schrödinger-Str. 52\, Kaiserslautern\, 67663\, Germany
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20260916
DTEND;VALUE=DATE:20260919
DTSTAMP:20260403T142309Z
CREATED:20260403T142309Z
LAST-MODIFIED:20260403T142309Z
UID:10000056-1789516800-1789775999@discover.restek.com
SUMMARY:CFIB (Conférence Francophone sur les Ingrédients Botaniques) 2026
DESCRIPTION:
URL:https://discover.restek.com/event/cfib-conference-francophone-sur-les-ingredients-botaniques-2026/
LOCATION:Théâtre et Palais des Congrès de Grasse\, 22 Cr Honoré Cresp\, Grasse\, 06130\, France
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20260916
DTEND;VALUE=DATE:20260922
DTSTAMP:20260403T141908Z
CREATED:20260403T141908Z
LAST-MODIFIED:20260403T141908Z
UID:10000055-1789516800-1790035199@discover.restek.com
SUMMARY:Mülheimer Wasseranalytisches Seminar 2026
DESCRIPTION:
URL:https://discover.restek.com/event/mulheimer-wasseranalytisches-seminar-2026/
LOCATION:Stadthalle Mülheim an der Ruhr\, Theodor-Heuss-Platz 1\, Mülheim an der Ruhr\, 45479\, Germany
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20260917
DTEND;VALUE=DATE:20260919
DTSTAMP:20260422T132020Z
CREATED:20260422T132020Z
LAST-MODIFIED:20260422T132020Z
UID:10000299-1789603200-1789775999@discover.restek.com
SUMMARY:CCCTA - Journées Scientifiques 2026
DESCRIPTION:
URL:https://discover.restek.com/event/cccta-journees-scientifiques-2026/
LOCATION:The Glacier Hotel\, Le Vernex 3\, Les Diablerets\, 1865\, Switzerland
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20260919
DTEND;VALUE=DATE:20260925
DTSTAMP:20260902T204740Z
CREATED:20260323T194524Z
LAST-MODIFIED:20260902T204740Z
UID:10000037-1789776000-1790294399@discover.restek.com
SUMMARY:SOFT 2026
DESCRIPTION:We’re going to SOFT 2026 in Chicago\, IL! Stop by Booth 313 to talk with our team about our solutions for forensics analysis. And don’t forget to enter our raffle for a chance to win an iPad (128 GB)! \nRestek’s Presentations at SOFT 2026\nOral Presentations\nEvaluating Strategies for Low-Level Quantification of THC Isomers and Metabolites in Whole Blood by Conventional GC-MS Instrumentation\nThursday\, September 24\, 9:10-9:20 a.m. | O-118\nPresenter/Authors: Amanda Rigdon\, Haley Berkland \nAbstract \nIntroduction: Liquid chromatography–tandem mass spectrometry (LC-MS/MS) is the preferred technique for drug and metabolite analysis in biological matrices due to its sensitivity\, selectivity\, and minimal derivatization requirements. However\, analysts may need to develop methods using conventional gas chromatography–mass spectrometry (GC-MS) due to limited LC-MS/MS access and the widespread availability of GC-MS platforms. \nObjectives: The objective of this work was to evaluate and mitigate the challenges of low-level drug analysis in biological matrices using conventional GC-MS through comprehensive method optimization. Analysis of THC isomers and metabolites in whole blood served as a representative test case due to the low detection limits required. Eleven analytes\, including 11-hydroxy-Δ8-tetrahydrocannabinol; 9(R)-hexahydrocannabinol; and (6aR\,9R)-Δ10-tetrahydrocannabinabinol were analyzed. \nMethods: Thermodynamic modeling software was employed to develop an optimized chromatographic separation on a 5-type column. A selected ion monitoring (SIM) method was established to optimize dwell times and leverage shared fragment ions between analytes. Following method development\, a calibration curve was prepared in ethyl acetate to evaluate the sensitivity of a conventional GC-MS system (Agilent 7890B GC coupled with a 5977B MS\, standard electron ionization source). Method performance was subsequently assessed in whole blood samples prepared via liquid–liquid extraction (LLE) and spiked post-extraction to evaluate sensitivity and selectivity in a complex matrix. Whole blood was acidified with 10% acetic acid\, then extracted with 9:1 hexane:ethyl acetate. Extracts were then spiked\, dried\, and derivatized with BSTFA + 1% TMCS. \nResults: All analytes demonstrated acceptable sensitivity in solvent at a final extract concentration of 5 ng/mL\, corresponding to 0.5 ng/mL in whole blood. Calibration curves exhibited acceptable linearity\, with r² values greater than 0.9990 for all analytes except Δ9-THC (r² = 0.9982). Elevated back-calculated accuracies were observed for four analytes at the low calibration range. As expected\, matrix interferences were significant for several monitored ions in whole blood. Acceptable sensitivity (S/N > 5) was achieved at 1 ng/mL in whole blood for at least one ion per analyte\, with the exception of (6aR\,9R)-Δ10-tetrahydrocannabinol and 11-nor-9-carboxy-Δ8-tetrahydrocannabinol\, which showed substantial interference across all monitored ions. Although less sensitive than LC-MS/MS\, this LOD is applicable to established working ranges for these analytes in whole blood. Triplicate injections of a post-extraction spiked sample (equivalent to 25 ng/mL in whole blood) yielded %RSD values of 1.0–10.6% after internal standard normalization. \nDiscussion: These results demonstrate that while conventional GC-MS systems cannot achieve the performance of advanced triple quadrupole platforms\, they can support low-level quantification of cannabinoids and related metabolites in biological matrices when methods are carefully optimized. Key factors influencing performance include chromatographic resolution for analytes as well as matrix interferences\, mass spectrometric parameter optimization\, and instrument maintenance. Increased variability at the low end of the calibration range highlights the need for further refinement to achieve robust quantification for all analytes. Ongoing work building on these results will be presented\, exploring the use of thermodynamic modeling to better characterize and mitigate matrix interferences as well as alternative derivatization strategies and detector optimization to enhance sensitivity. \nRecommendations for Expanding the Scope of an Existing LC-MS/MS Method\nThursday\, September 24\, 11:45-11:50 a.m. | L-016\nPresenter: Samantha Herbick \nIntroduction: Emerging drugs of abuse compounds are a consistent pain point for toxicology laboratories. There are often new analytes and novel psychoactive compounds (NPS) emerging every quarter. To stay current\, it is important for labs to update their testing panel\, but finding ways to keep methods up to date can be both time-consuming and challenging. This work provides recommendations to make these updates easier. Using emerging compounds from the Center for Forensic Research and Education’s (CFSRE) Scope Recommendations\, 20 compounds were selected to be added to an existing LC-MS/MS method that contained 231 analytes. Recommendations range from analyte selection\, metabolite considerations\, instrument method modifications\, and the use of a virtual chromatogram modeler. \nObjectives: The objective of this work is to offer recommendations to testing laboratories on easier ways to expand their testing scope when constantly dealing with new and emerging compounds. \nMethods: An existing LC-MS/MS screening method was chosen because of its extensive scope of 231 analytes. The method used a Biphenyl 100 x 2.1 mm\, 2.7 um column paired with a Biphenyl Guard Column Cartridge 5 x 2.1 mm\, 2.7 um. All existing analytes were separated using a 12-minute cycle time. Gradient conditions were used at 30 °C using water and methanol\, both containing 0.1% formic acid and 2 mM ammonium formate. The 20 analytes were added to the method and tested under optimized method conditions first by using a virtual chromatogram modeler to test retention and separation of the new analytes. A few of the new analytes were isobars with existing analytes in the original method. For example\, a-methyl acetyl fentanyl from the expansion list and fentanyl from the original list. Because of the different sets of isobars. method parameters were also tested to ensure resolution. These method changes included modifications to the column temperature and gradient conditions with the help of a virtual chromatogram modeler. \nResults: Analyte selection was in accordance with the Scope Recommendations provided by the CFSRE. Twenty of the newest emerging compounds were tested on an existing screening method to provide an example of elution order as well as isobar separations. The use of a virtual chromatogram modeler can be beneficial in instances like these. Ease of use and application of the modeler was demonstrated. Due to specific isobar groups\, additional modified method parameters were provided to address these separations as necessary. All additional analytes retained well on the existing method. \nDiscussion: Toxicology labs are constantly dealing with identification and testing of emerging compounds. It is often difficult and time-consuming to update existing methods. The recommendations included here in this work are intended to ease the burden of scope additions while retaining the integrity of the existing method. Resources\, such as the CFSRE\, are extremely helpful tools when considering what additions should be added to the testing scope. This resource paired with the recommendations above should help with toxicology labs future scope expansions. \nTechnical Posters\nLC-MS/MS Methods for Alcohol Biomarkers in Urine and Whole Blood\nTuesday\, September 22\, 12:00-1:30 p.m. | P112\nPresenter/Authors: Samantha Herbick\, Jamie York\, Justin Steimling \nIntroduction: Several established biomarkers are available for monitoring prior alcohol consumption in biological samples using LC-MS/MS. Selection of the appropriate biomarker depends on clinical objectives\, including detection of recent alcohol use (within ~3 days) or assessment of longer-term consumption (2–4 weeks). This study presents two analytical methods\, one for quantifying ethyl glucuronide (EtG) and ethyl sulfate (EtS) in urine\, and another for the analysis of phosphatidylethanol (PEth) in whole blood\, providing complementary tools for alcohol monitoring. \nObjectives: To develop and demonstrate two LC-MS/MS workflows for alcohol metabolite biomarkers in biological matrices: one workflow for quantifying short-term alcohol use biomarkers (EtG and EtS) in urine and another method for assessing drinking patterns through PEth analysis in whole blood. \nMethods: Analyses were performed using a Shimadzu LC-MS 8060 triple quadrupole mass spectrometer operating in ESI negative mode. \nFor urine analysis\, nine EtG and EtS calibrators (50–2\,000 ng/mL) were prepared in synthetic urine. Quality control samples were generated at 100\, 400\, and 1600 ng/mL using six unique lots of analyte-free human urine\, including two kidney disease patients in order to evaluate samples with enhanced potential for matrix effects. Samples were diluted 20-fold with water containing 0.1% formic acid\, vortexed\, centrifuged\, and injected (10 μL). Chromatographic separation was achieved using a Force Biphenyl column (100 × 3 mm\, 3 μm) with water and methanol (both containing 0.1% formic acid) as mobile phases. The flow rate was 0.8 mL/min\, the column oven was set to 30 °C\, and the overall run time was 5 minutes utilizing gradient conditions. \nFor whole blood analysis\, nine PEth homologue 16:0/18:1 and 16:0/18:2 calibrators were prepared in bovine whole blood at a range of 10-1000 ng/mL with quality controls prepared at 15\, 100\, and 500 ng/mL in analyte-free human whole blood. Samples (50 μL) were extracted with 150 μL of 4:1 isopropanol:tetrahydrofuran\, vortexed\, centrifuged\, and injected (5 μL). Separation was performed using a Raptor C8 column (50 × 2.1 mm\, 2.7 μm) with mobile phases consisting of water with 5 mM ammonium acetate and acetonitrile/isopropanol (90:10). The flow rate was 0.6 mL/min\, the column oven was set to 30 °C\, and the overall run time was 5 minutes using gradient conditions. \nResults: Both methods demonstrated excellent linearity\, with correlation coefficients (r²) ≥ 0.995. Calibration for EtG and EtS utilized 1/x-weighted quadratic regression\, whereas PEth was calibrated using 1/x-weighted linear regression. Intraday precision and accuracy for EtG and EtS showed percent recoveries ranging from 85.1-114.6%\, with %RSD values ≤9.6%. For PEth\, percent recoveries were within 10% of the nominal concentration\, with %RSD values ≤14.2%. Interday performance for both methods showed percent recoveries within 11.9% of the nominal range\, with %RSD values ≤10.1%. \nDiscussion: Two robust LC-MS/MS methods were successfully developed for the quantification of alcohol biomarkers in urine and whole blood. Both workflows are rapid\, sensitive\, and reliable\, meeting the performance requirements for clinical and forensic applications\, and enabling flexible monitoring of both short- and long-term alcohol consumption. \nSemisynthetic Cannabinoids: The Newest Challenge in Analyzing THC Isomers\nTuesday\, September 22\, 12:00-1:30 p.m. | P117\nPresenter/Authors: Jared Burkhart\, Haley Berkland\n \nIntroduction: Over the past several years\, Δ8-THC has made headlines as a popular “legal” alternative to Δ9-THC. The rise in popularity of Δ8-THC led to a new analytical challenge for toxicology laboratories—differentiating between the Δ8 and Δ9 isomer forms. For labs performing this testing by LC-MS/MS\, complete separation of these isomers and their metabolites is necessary for accurate quantitation. Recently\, a new group of compounds referred to as “semisynthetic cannabinoids”\, have emerged on the market. Drug monitoring groups in the U.S. have suggested that these compounds be added to toxicology testing scopes due to increased use and detection. Many toxicology laboratories have already implemented LC-MS/MS methods to simultaneously analyze for Δ8-THC and Δ9-THC in casework. In this work\, eight emerging semisynthetic cannabinoids (9(R)-HHC; 9(S)-HHC; 9(R)-HHC-COOH; 9(S)-HHC-COOH; Δ10-THC; Δ9-THC-O-Acetate; Δ9-THCP’ and CBDP) were added to an existing LC-MS/MS method used for the analysis of Δ8-THC\, Δ9-THC\, and their hydroxy and carboxy metabolites in whole blood. \nObjectives: Incorporate eight emerging semisynthetic cannabinoids into an existing method developed for analysis of THC isomers in whole blood. \nMethods: The original method was developed for the quantitative analysis of Δ8-THC\, Δ9-THC and their isomeric metabolites in whole blood by LC-MS/MS. The method used a Raptor FluoroPhenyl 100 x 3 mm\, 2.7 µm column and mobile phases of water and methanol\, both acidified with 0.1% formic acid. The column temperature was 40 °C\, the flow rate was 0.8 mL/min\, and gradient elution was used. A sample containing the eight semisynthetic cannabinoids and the original six cannabinoids was prepared at a concentration of 100 ng/mL. The sample was run on the original method to determine separation and elution order. \nResults: While resolution was achieved for all compounds\, including isomers\, using the original method conditions\, Δ10-THC\, Δ9-THCP\, and Δ9-THC-O-acetate eluted at 100% B\, where matrix interferences are known to wash off the column. To prevent interference\, the method conditions were altered to allow these compounds more time on the column. Additional resolution for the isomers was also achieved by dropping the column temperature from 40 °C to 30 °C. All critical pairs had a calculated resolution of 1.5 or better\, demonstrating baseline resolution was achieved. \nDiscussion: All eight semisynthetic cannabinoids were successfully integrated into a preexisting LC-MS/MS panel for the analysis of THC isomers in whole blood. Because different isomer configurations can have different psychoactive activities\, it is important to differentiate between isomer forms in toxicology casework. This work highlights the importance of choosing a stationary phase that shows optimal selectivity for the analyte class being analyzed\, allowing new analytes to easily be integrated into the method as they emerge. \nAnalyzing the Impact of Centrifugation and Extraction Aides on Oral Fluid Sample Recovery\nWednesday\, September 23\, 12:00-1:30 p.m. | P265\nPresenter/Authors: Samantha Herbick\, Jared Burkhart \nIntroduction: Oral fluid is a relatively simple matrix to collect and prepare; however\, the collection kits can cause issues downstream. Quantisal™ collection kits consist of an applicator with attached sponge\, a tube with buffer solution\, and a cap. The total volume for testing should be 4 mL (1 mL of oral fluid and 3 mL of buffer); however\, it is difficult to recover the full amount. There are different techniques to manipulate the sponge and improve sample recovery. Popular techniques include manual compression with and without and aide and centrifugation. This work also tested and compared an oral fluid extraction accessory (OFEA) accompanied with a centrifugation step to previously mentioned techniques. This study compared these four extraction techniques by examining volume recovery and analyte recovery. \nObjectives: Demonstrate the advantages of incorporating a centrifugation step and the use of extraction aides to sample preparation to determine its impacts on volume and analyte recovery when performing analysis of drugs of abuse and (DoA) in oral fluids by LC-MS/MS. \nMethods: Thirty-one common DoA compounds were separated by LC-MS/MS using a Biphenyl column under gradient conditions using water and methanol\, both containing 0.1% formic acid\, with a total cycle time of 7 minutes. Samples were prepared in synthetic oral fluid and combined with Quantisal™ buffer. Four recovery techniques were compared and tested in triplicate: manual compression\, centrifugation\, manual compression with an aide\, and centrifugation with OFEA. Samples were prepared using a salt-assisted liquid-liquid extraction (SALLE). \nResults: Four techniques were evaluated by examining total volume removed and analyte recovery. Volume recovery was tested by pouring the solution into a graduated cylinder and recording the total volume recovered. When using centrifugation with the OFEA\, an additional 500 µL was collected compared to the other techniques. Analyte recovery was compared by spiking a known concentration\, 50 ng/mL\, into each of the samples. Samples were evaluated using a calibration curve prepared without using the kits. Peak areas increased for all analytes when using the centrifugation and centrifugation with the OFEA. Accuracy and precision were assessed by analyzing whether the results fell within +/- 15% of the target as well as comparing %RSD values. Manual compression with the aide and centrifugation with the OFEA showed the best accuracy and precision with comparable results to each other. A study was also conducted to assess efficiency of each of the techniques. Four samples were prepared by each technique\, and the time was then multiplied to give an indication of time for 12\, 48\, and 96 samples. When preparing 96 samples\, the centrifugation with the OFEA is the fastest technique by up to 32 minutes. \nDiscussion: Four techniques were compared to extract liquid from the sponge of oral fluid collection kits. Through examining volume and analyte recovery\, it was clear that the centrifugation step paired with the oral fluid extraction accessory showed the best results for total volume recovered as well as increased analyte recovery when analyzed quantitatively compared to the other techniques. \nEnter Our Raffle!\nEnter our SOFT raffle for a chance to win an Apple iPad (128 GB)!
URL:https://discover.restek.com/event/soft-2026/
LOCATION:Hilton Chicago\, 720 South Michigan Avenue\, Chicago\, IL\, 60605\, United States
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SUMMARY:Farmaforum 2026
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URL:https://discover.restek.com/event/farmaforum-2026/
LOCATION:IFEMA – Feria de Madrid\, Pavilion 10\, Av. Partenón 5\, Madrid\, 28042\, Spain
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SUMMARY:Live Webinar: From Challenge to Confidence: Advanced GC Column Strategies for Environmental & Food Testing
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SUMMARY:MSACL (Mass Spectrometry & Advances in the Clinical Lab) 2026
DESCRIPTION:
URL:https://discover.restek.com/event/msacl-mass-spectrometry-advances-in-the-clinical-lab-2026/
LOCATION:Bonaventure Hotel Conference Center\, 900 Rue De la Gauchetière O\, Montréal\, QC\, H5A 1E4\, Canada
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SUMMARY:ChromSAAMS 2026
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LOCATION:Zebula Golf Estate & Spa\, Farm 534 Mabula\, Bela-Bela\, 0480\, South Africa
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SUMMARY:Gulf Coast Conference 2026
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URL:https://discover.restek.com/event/gulf-coast-conference-2026/
LOCATION:Moody Gardens Convention Center\, One Hope Blvd\, Galveston\, TX\, United States
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SUMMARY:4th European Sample Preparation Conference (EuSP2026) & 3rd Green and Sustainable Analytical Chemistry Conference (GSAC2026)
DESCRIPTION:
URL:https://discover.restek.com/event/4th-european-sample-preparation-conference-eusp2026-3rd-green-and-sustainable-analytical-chemistry-conference-gsac2026/
LOCATION:Universidad de Santiago de Compostela\, Praza do Obradoiro\, Santiago de Compostela\, 15705\, Spain
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URL:https://discover.restek.com/event/eas-eastern-analytical-symposium-2026/
LOCATION:Crowne Plaza Princeton Conference Center\, 900 Scudders Mill Rd\, Plainsboro\, NJ\, United States
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SUMMARY:SWAFS (Southwestern Association of Forensic Scientists) 2026
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URL:https://discover.restek.com/event/swafs-southwestern-association-of-forensic-scientists-2026/
LOCATION:Grand Junction Convention Center\, 159 Main St\, Grand Junction\, CO\, United States
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SUMMARY:PRAXISTAG HPLC 2026
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URL:https://discover.restek.com/event/praxistag-hplc-2026/
LOCATION:Veranstaltungsforum Fürstenfeld\, Fürstenfeld 12\, Fürstenfeldbruck\, 82256\, Germany
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