FAQ

How are SPE cartridges processed?

30 Jul 2025

Cartridges may be processed by any of the techniques shown below.

a diagram of a vacuum manifold, positive pressure technique, and a vacuum-sidearm flask

A. Vacuum Manifold
The most common way to process SPE cartridges is to use an SPE vacuum manifold along with a vacuum source, such as a house vacuum, or a Chemker oil-free vacuum/pressure station. If you are using a well plate SPE product, a vacuum manifold specifically designed for well plates, such as the Resprep VM-96 vacuum manifold, can be used to process your SPE well plate.

B. Positive Pressure
The positive pressure technique uses an appropriate syringe (with a male luer fitting) along with the correct size SPE cartridge connector. An electronically controlled or air-actuated positive pressure system can also be used to process either a well plate or multiple cartridges at once.

C. Vacuum-Sidearm Flask
A vacuum-sidearm flask, along with a water faucet for aspiration, takes advantage of the Venturi effect. A standard vacuum source can also be used.

Author

  • Restek Corporation

    Restek is a leading provider of chromatography columns, accessories, and certified reference materials. Trust Restek for reliable, high-quality analytical solutions.

    View all posts
GNOT5147