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PFAS Analysis by U.S. USDA CLG-PFAS 2.04 for Bovine, Porcine, Poultry, Siluriformes Muscle, and Bovine Plasma

03 Oct 2025

feature FSAR4290
  • Sample Matrix: bovine, porcine, poultry, and Siluriformes muscle and bovine plasma
  • Sample Preparation: protein precipitation extraction
  • Detection Technique: UHPLC-MS/MS

Screening, Determination, and Confirmation of PFAS by UHPLC-MS-MS

USDA Method CLG-PFAS 2.04 is used for the analysis of 16 PFAS in bovine, porcine, poultry, Siluriformes muscle samples, and in bovine plasma samples. It combines a methanol-based protein precipitation extraction with UHPLC-MS/MS analysis on a C18 column. The method is performance based and allows equivalent equipment, reagents, or solutions to be used, and some instrument parameters can be optimized as detailed in the method to improve chromatography and detection. The full analyte list and recommended products for PFAS analysis in bovine, porcine, poultry, Siluriformes muscle, and bovine plasma samples by CLG-PFAS 2.04 are provided below. In addition, you can register and directly compare analyte lists and matrices across PFAS analysis methods here or visit www.restek.com/PFAS to explore our technical resources for PFAS analysis.

Analyte List for USDA CLG-PFAS 2.04 for PFAS Analysis

PFAS acronyms, compound names, and CAS numbers vary across methods and guidelines, and they also depend on compound form (e.g., neutral vs. salt). The following list uses standardized terminology to allow easy comparison across the methods and guidelines in our comprehensive guide. Please refer to U.S. USDA CLG-PFAS 2.04 for method-specific nomenclature.

AcronymCompound NameCAS#
PFBSPerfluoro-1-butanesulfonic acid375-73-5
PFDAPerfluorodecanoic acid335-76-2
PFDoAPerfluorododecanoic acid307-55-1
PFHpAPerfluoroheptanoic acid375-85-9
PFHxSPerfluoro-1-hexanesulfonic acid355-46-4
PFHxAPerfluorohexanoic acid307-24-4
PFNAPerfluorononanoic acid375-95-1
PFOSPerfluorooctanesulfonic acid1763-23-1
PFOAPerfluorooctanoic acid335-67-1
PFTeDAPerfluorotetradecanoic acid376-06-7
PFTrDAPerfluorotridecanoic acid72629-94-8
PFUnAPerfluoroundecanoic acid2058-94-8
PFPeAPerfluoropentanoic acid2706-90-3
PFDSPerfluoro-1-decanesulfonic acid335-77-3
PFHxDAPerfluoro-n-hexadecanoic acid67905-19-5
PFODAPerfluoro-n-octadecanoic acid16517-11-6

Recommended Products for U.S. USDA CLG-PFAS for PFAS Analysis

Analytical LC Columns:


Colonne Raptor C18 2.7µm, 50 x 2.1 mm
Colonne LC Force C18, 1,8 μm 50 mm x 2.1 mm

Delay Columns:


“Colonne-retard” PFAS HPLC, 5 µm, 50 x 2.1 mm

PFAS delay column: 5 µm, 50 x 3.0 mm (cat.# 580959 [custom product])

Guard Columns:


Filtre/précolonne UltraShield UHPLC, fritté 0.2 μm, l’unité
Support de cartouche à connexion directe EXP, livré avec un écrou et 2 ferrules
Cartouche de garde Raptor C18 EXP, 2.7 µm, L 5 x DI 2.1 mm, lot de 3

Standards:


Solution-étalon de calibration PFAS EPA 537.1, 2 μg/ml de chaque composé dans le méthanol (1 mM KOH), 1 ml/ampoule
Discontinued: Solution-étalon de calibration PFAS EPA 533, 2 μg/ml de chaque composé dans le méthanol (1 mM KOH), 1 ml/ampoule

Sample Prep:


Centrifugeuse Q-sep Multispeed pour QuEChERS

Sample Reservoirs:


Tubes vides en polypropylène pour SPE, réservoir de 75 ml, lot de 12
Connecteurs pour cartouches SPE, 1, 3, 6, 10 ou 15 ml, polypropylène, lot de 15

Vacuum Manifolds:


Manifold sous-vide Resprep QR-24
Manifold sous-vide Resprep QR-12

Vacuum Pumps:


Pompe à vide Chemker 300 en PTFE, 20 L/min, AC110 V, 60 Hz
Pompe à vide Chemker 300 PTFE, 18 l/min, 220 V, 50 Hz

General Lab Supplies:


Flacon à volume limité à visser, polypropylène, 9 mm, 700 μl, 12 x 32 mm, lot de 100
Flacon à volume limité à visser, polypropylène, 9 mm, 700 μl, 12 x 32 mm, lot de 1000
Bouchon à visser, polyéthylène (solide), membrane de 0.254 mm, transparent, 2.0 ml, 9 mm, lot de 100
Bouchon à visser, polyéthylène (solide), membrane de 0.254 mm, transparent, 2.0 ml, 9 mm, lot de 1000
Tubes vides en polypropylène pour centrifugation de 50 ml, avec bouchons, lot de 50
Tubes vides en polypropylène pour centrifugation de 50 ml, avec bouchons, lot de 500
Seringues NORM-JECT, 10 ml, embout « luer lock », lot de 100
Seringues NORM-JECT, 5 ml, embout « luer lock », lot de 100

Discover More Solutions for PFAS Analysis!

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Author

  • Restek Corporation

    Restek is a leading provider of chromatography columns, accessories, and certified reference materials. Trust Restek for reliable, high-quality analytical solutions.

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FSAR4290
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