In this scenario, the sample is initially concentrated onto the head of the column and moves through the column in a tight band. This technique, referred to as “on-column compression” or “point-injected,” is sometimes used to minimize band broadening for a larger volume sample injection. Keep in mind that your sample components must be soluble in mobile phase as well in order for this to work.
Can I get a sharper peak by injecting my sample in a weaker injection solvent (such as 100% water for reverse phase)?
31 Jul 2025
Author
GNOT5054
