A small amount of phase bleed is inherent for all phases, including phenyl phases, and is somewhat dependent on the size and dimensions of the column. This bleed is usually negligible and does not affect retention times but may be visible, particularly by UV detection. It can often be reduced after conditioning. Bleed may also be minimized by using an isocratic elution, a shallower gradient, and/or incorporating a gradient flush between runs.
Why am I seeing bleed from my Biphenyl column on my UV but not on my mass spec?
31 Jul 2025
Author
GNOT5057
