Typically, a 3:1 ratio of organic solvent to sample is used. Adding more organic solvent may produce better protein precipitation, but the additional solvent should be calculated into the dilution factor. The analyte concentration in the final filtrate will be diluted by the actual solvent:sample ratio that is used.
What should the organic solvent:sample ratio be for effective precipitation?
30 Jul 2025
Author
GNOT5179
