Over the past several years, Δ8-THC has made headlines as a popular “legal” alternative to Δ9-THC. The rise in popularity of Δ8-THC led to a new analytical challenge for toxicology laboratories—differentiating between the Δ8 and Δ9 isomer forms. For labs performing this testing by LC-MS/MS, complete separation of the isomers and their metabolites is necessary for accurate quantitation. While HPLC stationary phases like Biphenyl and C18 have traditionally been used for toxicological analysis of cannabinoids, they lack the selectivity needed to adequately separate these isomers. FluoroPhenyl phase columns have emerged as a superior choice for analyzing THC isomers in biological matrices due to their unique selectivity capabilities.
Semisynthetic Cannabinoids: The Newest Challenge in Analyzing THC Isomers
03 Mar 2026
Author
GNOT5637
