Large Volume Injection of Pesticides in Matrix on Low-Pressure Gas Chromatography

Concurrent Solvent Recondensation Large Sample Volume Splitless Injection (CSR-LVSI, or LVI) is a gas chromatography sample injection technique that overcomes the limitation of using a smaller sample volume of 1to 2 μL, which is typical of conventional splitless injections. Low-Pressure Gas Chromatography (LPGC) is a novel technique that has been successfully applied for multiresidue pesticide detection and quantification. The LPGC configuration with the restrictor/guard column facilitates the requirements of a CSR-LVSI and has a potential to improve the sensitivity and lower detection limits. Peak shapes of large volume injections using both acetonitrile and acetonitrile-toluene were evaluated in the range of 5–50 μL, and the relationship between the peak area and injection volume was established. The calibration parameters were compared for 5, 15, and 25 μL injection insolvent and matrix. The limits of detection and other parameters were compared between the two injection volumes.