{"id":2948,"date":"2025-07-30T18:39:12","date_gmt":"2025-07-30T18:39:12","guid":{"rendered":"https:\/\/discover.restek.com\/?p=2948"},"modified":"2025-12-08T23:20:08","modified_gmt":"2025-12-08T23:20:08","slug":"how-are-spe-cartridges-processed","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/fr\/faq\/gnot5147\/how-are-spe-cartridges-processed","title":{"rendered":"How are SPE cartridges processed?"},"content":{"rendered":"\n<p>Cartridges may be processed by any of the techniques shown below.<\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNOT3399-04.jpg\" alt=\"a diagram of a vacuum manifold, positive pressure technique, and a vacuum-sidearm flask\" class=\"wp-image-2909\" style=\"width:730px;height:auto\" title=\"-\"><\/figure>\n\n\n\n<p><strong>A. Vacuum Manifold<\/strong><br>The most common way to process SPE cartridges is to use an SPE vacuum manifold along with a vacuum source, such as a house vacuum, or a Chemker oil-free vacuum\/pressure station. If you are using a well plate SPE product, a vacuum manifold specifically designed for well plates, such as the&nbsp;<a href=\"https:\/\/www.restek.com\/p\/25858\" target=\"_blank\" rel=\"noopener\">Resprep VM-96 vacuum manifold<\/a>, can be used to process your SPE well plate.<\/p>\n\n\n\n<p><strong>B. Positive Pressure<\/strong><br>The positive pressure technique uses an appropriate syringe (with a male luer fitting) along with the correct size SPE cartridge&nbsp;<a href=\"https:\/\/www.restek.com\/c\/1349?Ns=restekProduct.x_productBadge%7C0\" target=\"_blank\" rel=\"noopener\">connector<\/a>. An electronically controlled or air-actuated positive pressure system can also be used to process either a well plate or multiple cartridges at once.<\/p>\n\n\n\n<p><strong>C. Vacuum-Sidearm Flask<\/strong><br>A vacuum-sidearm flask, along with a water faucet for aspiration, takes advantage of the&nbsp;<a href=\"https:\/\/en.wikipedia.org\/wiki\/Venturi_effect\" target=\"_blank\" rel=\"noreferrer noopener\">Venturi effect<\/a>. A standard vacuum source can also be used.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Cartridges may be processed by any of the techniques shown below. A. Vacuum ManifoldThe most common way to process SPE cartridges is to use an SPE vacuum manifold along with a vacuum source, such as a house vacuum, or a Chemker oil-free vacuum\/pressure station. If you are using a well plate SPE product, a vacuum&#8230;<\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[23],"tags":[2688,2708],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[],"hf_cat_post":[625],"ppma_author":[414],"class_list":["post-2948","post","type-post","status-publish","format-standard","hentry","category-faq","tag-faq-sample-preparation","tag-faq-solid-phase-extraction-spe"],"acf":[],"taxonomy_info":{"category":[{"value":23,"label":"FAQ"}],"post_tag":[{"value":2688,"label":"FAQ: Sample Preparation"},{"value":2708,"label":"FAQ: Solid Phase Extraction (SPE)"}]},"featured_image_src_large":false,"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/fr\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":23,"name":"FAQ","slug":"faq","term_group":0,"term_taxonomy_id":23,"taxonomy":"category","description":"","parent":0,"count":271,"filter":"raw","cat_ID":23,"category_count":271,"category_description":"","cat_name":"FAQ","category_nicename":"faq","category_parent":0}],"tag_info":[{"term_id":2688,"name":"FAQ: Sample Preparation","slug":"faq-sample-preparation","term_group":0,"term_taxonomy_id":2688,"taxonomy":"post_tag","description":"Browse frequently asked questions for sample preparation products.","parent":0,"count":52,"filter":"raw"},{"term_id":2708,"name":"FAQ: Solid Phase Extraction (SPE)","slug":"faq-solid-phase-extraction-spe","term_group":0,"term_taxonomy_id":2708,"taxonomy":"post_tag","description":"Browse frequently asked questions for SPE products.","parent":0,"count":9,"filter":"raw"}],"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/2948","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/comments?post=2948"}],"version-history":[{"count":3,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/2948\/revisions"}],"predecessor-version":[{"id":70455,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/2948\/revisions\/70455"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/media?parent=2948"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/categories?post=2948"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/tags?post=2948"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/industries-application?post=2948"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/post-badge?post=2948"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/resource-type?post=2948"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/product-library?post=2948"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/resource-technique?post=2948"},{"taxonomy":"hf_cat_post","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/hf_cat_post?post=2948"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/ppma_author?post=2948"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}