To ensure proper mixing, we recommend vortexing for 0.5\u20132 min at a minimum speed of 2000 rpm. The mixing time will depend on solvent volume, sample volume, and sample type. If protein removal is incomplete, increase the mixing time and\/or the vortex speed (up to 3000 rpm).<\/p>\n","protected":false},"excerpt":{"rendered":"
To ensure proper mixing, we recommend vortexing for 0.5\u20132 min at a minimum speed of 2000 rpm. The mixing time will depend on solvent volume, sample volume, and sample type. 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