{"id":42475,"date":"2019-11-12T05:00:00","date_gmt":"2019-11-12T05:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/quel-solvant-d-injection-utiliser-en-mode-hilic\/"},"modified":"2026-01-27T21:28:24","modified_gmt":"2026-01-27T21:28:24","slug":"quel-solvant-d-injection-utiliser-en-mode-hilic","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/fr\/videos-fr\/gnav3613-fr\/quel-solvant-d-injection-utiliser-en-mode-hilic","title":{"rendered":"Quel solvant d&#8217;injection utiliser en mode HILIC ?"},"content":{"rendered":"\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"Injection Solution Conditions for HILIC\" width=\"720\" height=\"405\" src=\"https:\/\/www.youtube.com\/embed\/iX3Tm2v9DGU?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Description<\/h2>\n\n\n\n<p>(sous-titres disponibles en fran\u00e7ais)<\/p>\n\n\n\n<p>La chromatographie liquide d\u2019interaction hydrophobe, ou mode HILIC, utilise des conditions de phase mobile contenant un fort pourcentage de solvant organique. Il est donc tr\u00e8s important de s\u2019assurer que le solvant d\u2019injection soit compatible. Rejoignez-nous pour discuter de l\u2019importance du solvant d\u2019injection en mode HILIC.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Ressources compl\u00e9mentaires<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/discover.restek.com\/articles\/gnar2716\/how-to-avoid-common-problems-with-hilic-methods\" target=\"_blank\" data-type=\"link\" data-id=\"https:\/\/discover.restek.com\/GNAR2716\/how-to-avoid-common-problems-with-hilic-methods\/\" rel=\"noreferrer noopener\">Comment \u00e9viter les probl\u00e8mes courants avec les m\u00e9thodes HILIC ?<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/discover.restek.com\/articles\/gnss2687\/raptor-hilic-si-simplify-the-switch-to-hilic\" data-type=\"link\" data-id=\"https:\/\/discover.restek.com\/GNSS2687\/raptor-hilic-si-simplify-the-switch-to-hilic\/\" target=\"_blank\" rel=\"noreferrer noopener\">Simplifiez le passage en mode HILIC <\/a><a href=\"https:\/\/discover.restek.com\/articles\/gnss2687\/raptor-hilic-si-simplify-the-switch-to-hilic\" target=\"_blank\" data-type=\"link\" data-id=\"https:\/\/discover.restek.com\/fr\/articles-fr\/gnss2687\/raptor-hilic-si-simplify-the-switch-to-hilic\/\" rel=\"noreferrer noopener\">avec les colonnes Raptor HILIC-Si<\/a><\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Produits associ\u00e9s Restek<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.restek.com\/c\/1793\" target=\"_blank\" data-type=\"link\" data-id=\"https:\/\/www.restek.com\/c\/1793\" rel=\"noreferrer noopener\">Colonnes Raptor HILIC-Si<\/a><\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Transcription<\/h2>\n\n\n\n<p>Si vous avez l\u2019habitude de d\u00e9velopper des m\u00e9thodes LC en phase inverse, vous connaissez l\u2019influence que peut avoir le solvant d\u2019injection sur la forme des pics, particuli\u00e8rement pour les compos\u00e9s \u00e9luant tr\u00e8s t\u00f4t. L\u2019impact du solvant d\u2019injection est encore plus significatif pour les analyses en mode HILIC.<\/p>\n\n\n\n<p>Il est tr\u00e8s important d\u2019avoir un solvant d\u2019injection de m\u00eame composition que les conditions initiales de phase mobile, qui contiennent un fort pourcentage d\u2019organique. Comme vous pouvez le voir dans cet exemple, l\u2019analyse du diquat et du paraquat sur une colonne Raptor HILIC-Si, en comparaison \u00e0 l\u2019\u00e9chantillon dans un solvant d\u2019injection 100% aqueux, l\u2019\u00e9chantillon dissout dans une solution organique \u00e0 75%, identique aux conditions initiales de phase mobile, donne un chromatogramme avec non seulement une meilleure forme\/sym\u00e9trie de pics mais \u00e9galement une plus grande r\u00e9tention et une meilleure sensibilit\u00e9.<\/p>\n\n\n\n<p>Donc, pour r\u00e9sumer, les m\u00e9thodes en mode HILIC sont un outil puissant pour l\u2019analyse des compos\u00e9s polaires qui ne peuvent pas \u00eatre analys\u00e9s en phase inverse (\u00ab classique \u00bb). Assurez-vous juste de bien conditionner et r\u00e9\u00e9quilibrer la colonne avant et entre chaque injection, \u00e9valuer les effets du pH de la phase mobile et du choix du tampon sur votre analyse, et adapter le solvant d\u2019injection aux conditions initiales de phase mobile. Ces pr\u00e9conisations fonctionneront avec n\u2019importe laquelle des phases stationnaires de mode HILIC que vous choisirez d\u2019utiliser.<\/p>\n\n\n\n<p>Si vous appr\u00e9ciez ces vid\u00e9os, s\u2019il vous pla\u00eet, \u201clikez\u201d, partagez et inscrivez-vous. Vous pouvez proposer des id\u00e9es pour un futur conseil technique dans les commentaires ci-dessous.<\/p>\n\n\n\n<p>Nous esp\u00e9rons que vos d\u00e9veloppements de m\u00e9thodes HILIC seront d\u00e9sormais couronn\u00e9s de succ\u00e8s.<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Comprendre l&#8217;importance du solvant d&#8217;injection en mode HILIC.<\/p>\n","protected":false},"author":11,"featured_media":41132,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[771],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[2445],"resource-technique":[2354],"ppma_author":[414],"class_list":["post-42475","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-videos-fr","product-library-produits-pour-la-chromatographie-en-phase-liquide","resource-technique-hilic-fr"],"acf":[],"taxonomy_info":{"category":[{"value":771,"label":"Vid\u00e9os"}],"product-library":[{"value":2445,"label":"Produits pour la chromatographie en phase liquide"}],"resource-technique":[{"value":2354,"label":"HILIC"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-GNAV3613-1024x536.jpg",1024,536,true],"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/fr\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":771,"name":"Vid\u00e9os","slug":"videos-fr","term_group":0,"term_taxonomy_id":771,"taxonomy":"category","description":"","parent":0,"count":133,"filter":"raw","cat_ID":771,"category_count":133,"category_description":"","cat_name":"Vid\u00e9os","category_nicename":"videos-fr","category_parent":0}],"tag_info":false,"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/42475","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/comments?post=42475"}],"version-history":[{"count":8,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/42475\/revisions"}],"predecessor-version":[{"id":84996,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/42475\/revisions\/84996"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/media\/41132"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/media?parent=42475"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/categories?post=42475"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/tags?post=42475"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/industries-application?post=42475"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/post-badge?post=42475"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/resource-type?post=42475"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/product-library?post=42475"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/resource-technique?post=42475"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/ppma_author?post=42475"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}