{"id":42481,"date":"2019-05-21T04:00:00","date_gmt":"2019-05-21T04:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/rincage-et-regeneration-des-colonnes-lc\/"},"modified":"2026-01-28T21:46:57","modified_gmt":"2026-01-28T21:46:57","slug":"rincage-et-regeneration-des-colonnes-lc","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/fr\/videos-fr\/gnav3616-fr\/rincage-et-regeneration-des-colonnes-lc","title":{"rendered":"Rin\u00e7age et r\u00e9g\u00e9n\u00e9ration des colonnes LC"},"content":{"rendered":"\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"LC Column Cleaning and Regeneration\" width=\"720\" height=\"405\" src=\"https:\/\/www.youtube.com\/embed\/F4cdVXmjRw8?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Description<\/h2>\n\n\n\n<p>(sous-titres disponibles en fran\u00e7ais)<\/p>\n\n\n\n<p>Au fil du temps, nos colonnes de chromatographie liquide vont petit \u00e0 petit se contaminer, surtout lorsque nous analysons r\u00e9guli\u00e8rement des matrices charg\u00e9es. Mais nous pouvons prendre certaines mesures pour pr\u00e9server la propret\u00e9 de nos colonnes. Nous pouvons \u00e9galement de temps en temps les r\u00e9g\u00e9n\u00e9rer, ce qui peut aider \u00e0 restaurer certaines de leurs performances et \u00e0 prolonger leur dur\u00e9e de vie.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Ressources compl\u00e9mentaires<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/discover.restek.com\/instruction-sheet\/700-02-002\/restek-liquid-chromatography-lc-columns-usage-care\" target=\"_blank\" data-type=\"link\" data-id=\"https:\/\/discover.restek.com\/700-02-002\/restek-liquid-chromatography-lc-columns-usage-care\/\" rel=\"noreferrer noopener\">Recommandations pour le nettoyage d\u2019une colonne LC<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/discover.restek.com\/GNSS2975-FR\/selecteur-de-precolonnes-restek-comment-trouver-rapidement-la-bonne-protection\/\" target=\"_blank\" data-type=\"link\" data-id=\"https:\/\/discover.restek.com\/GNSS2975-FR\/selecteur-de-precolonnes-restek-comment-trouver-rapidement-la-bonne-protection\/\" rel=\"noreferrer noopener\">S\u00e9lecteur de pr\u00e9colonnes LC<\/a><\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Produits associ\u00e9s Restek<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.restek.com\/c\/1119\" target=\"_blank\" rel=\"noreferrer noopener\">Colonnes LC<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/c\/1277\" target=\"_blank\" rel=\"noreferrer noopener\">Cartouches et accessoires<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/c\/1123\" target=\"_blank\" rel=\"noreferrer noopener\">Accessoires pour phase mobile<\/a><\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Transcription<\/h2>\n\n\n\n<p>Merci de nous rejoint pour ce nouveau conseil Restek, dans lequel nous allons parler du rin\u00e7age et de la r\u00e9g\u00e9n\u00e9ration des colonnes LC. La contamination d\u2019une colonne due \u00e0 l\u2019adsorption de l\u2019analyte ou des impuret\u00e9s provenant de la matrice peut avoir des effets tr\u00e8s n\u00e9gatifs sur votre chromatographie, tels que des pics d\u00e9form\u00e9s, des changements de s\u00e9lectivit\u00e9 ou des pertes de sensibilit\u00e9, qui peuvent tous impacter la quantification. Il est fortement recommand\u00e9 d\u2019utiliser des pr\u00e9-colonnes ou un filtre pr\u00e9-colonne UltraShield pour prot\u00e9ger votre colonne analytique.<\/p>\n\n\n\n<p>Cependant, au fil du temps, et m\u00eame avec ces pr\u00e9cautions, on peut parfois observer une d\u00e9gradation des performances de la colonne, une augmentation de la contre-pression, ou d\u2019autres signes de contamination de la colonne. Pour le rin\u00e7age et la r\u00e9g\u00e9n\u00e9ration des colonnes HPLC et UHPLC, nous recommandons un rin\u00e7age \u00e0 l\u2019aide d\u2019une s\u00e9rie de combinaisons de solvants forts avec un d\u00e9bit dans le sens indiqu\u00e9 sur la colonne. Pour les colonnes en phase inverse, rincez la colonne avec un minimum de 20 volumes de colonne de chaque solvant, dans l\u2019ordre indiqu\u00e9 dans le tableau en commen\u00e7ant par 5% de m\u00e9thanol dans l\u2019eau et en allant jusqu\u2019\u00e0 une composition en solvants fortement organique puis revenez \u00e0 vos conditions initiales de phase mobile. Pour les colonnes de phase normale, rincez avec un minimum de 20 volumes de colonne de chaque solvant, dans l\u2019ordre indiqu\u00e9 dans le tableau en commen\u00e7ant par l\u2019IPA et en allant jusqu\u2019\u00e0 l\u2019\u00e9thanol puis revenez \u00e0 vos conditions initiales de phase mobile.<\/p>\n\n\n\n<p>Le volume de colonne peut \u00eatre calcul\u00e9 \u00e0 l\u2019aide de la formule indiqu\u00e9e ici. Il est grandement recommand\u00e9 de r\u00e9-\u00e9quilibrer votre colonne dans les conditions initiales de votre m\u00e9thode en effectuant une s\u00e9rie d\u2019injections avant de d\u00e9marrer votre s\u00e9quence d\u2019injections. Apr\u00e8s avoir r\u00e9g\u00e9n\u00e9r\u00e9 la colonne, il est important de stocker vos colonnes LC avec les bouchons solidement fix\u00e9s aux extr\u00e9mit\u00e9s et dans le solvant recommand\u00e9 par le fabricant, que ce soit pour un stockage \u00e0 court ou long terme.<\/p>\n\n\n\n<p>Si vous appr\u00e9ciez ces vid\u00e9os, vous pouvez \u00ab\u202fliker\u202f\u00bb et partager et vous abonner \u00e0 notre cha\u00eene. N\u2019h\u00e9sitez pas \u00e0 proposer des id\u00e9es pour un futur conseil technique dans les commentaires ci-dessous.<\/p>\n\n\n\n<p>Merci de nous avoir rejoint pour ce conseil Restek.<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Dans cette astuce Restek&#8221;, parlons chromatographie liquide et, plus sp\u00e9cifiquement, dur\u00e9e de vie des colonnes LC. Nous partageons avec vous nos conseils \u00e0 propos du rin\u00e7age et de la r\u00e9g\u00e9n\u00e9ration des colonnes LC.&#8221;<\/p>\n","protected":false},"author":11,"featured_media":41144,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[771],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[2463,2445],"resource-technique":[2318],"ppma_author":[414],"class_list":["post-42481","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-videos-fr","product-library-colonnes-lc","product-library-produits-pour-la-chromatographie-en-phase-liquide","resource-technique-chromatographie-en-phase-liquide"],"acf":[],"taxonomy_info":{"category":[{"value":771,"label":"Vid\u00e9os"}],"product-library":[{"value":2463,"label":"Colonnes LC"},{"value":2445,"label":"Produits pour la chromatographie en phase liquide"}],"resource-technique":[{"value":2318,"label":"Chromatographie en phase liquide"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-GNAV3616-1024x536.jpg",1024,536,true],"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/fr\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":771,"name":"Vid\u00e9os","slug":"videos-fr","term_group":0,"term_taxonomy_id":771,"taxonomy":"category","description":"","parent":0,"count":136,"filter":"raw","cat_ID":771,"category_count":136,"category_description":"","cat_name":"Vid\u00e9os","category_nicename":"videos-fr","category_parent":0}],"tag_info":false,"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/42481","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/comments?post=42481"}],"version-history":[{"count":4,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/42481\/revisions"}],"predecessor-version":[{"id":85726,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/42481\/revisions\/85726"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/media\/41144"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/media?parent=42481"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/categories?post=42481"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/tags?post=42481"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/industries-application?post=42481"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/post-badge?post=42481"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/resource-type?post=42481"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/product-library?post=42481"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/resource-technique?post=42481"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/ppma_author?post=42481"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}