{"id":43356,"date":"2021-09-29T00:00:00","date_gmt":"2021-09-29T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/simultaneous-analysis-of-alcohol-metabolites-and-barbiturates-by-lc-msms\/"},"modified":"2026-03-18T13:57:16","modified_gmt":"2026-03-18T13:57:16","slug":"simultaneous-analysis-of-alcohol-metabolites-and-barbiturates-by-lc-msms","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/fr\/application-notes-it\/cfan3719\/simultaneous-analysis-of-alcohol-metabolites-and-barbiturates-by-lc-msms","title":{"rendered":"Simultaneous Analysis of Alcohol Metabolites and Barbiturates by LC-MS\/MS"},"content":{"rendered":"\n

Abstract<\/h2>\n\n\n\n

Analysis of alcohol metabolites and barbiturates in urine typically is done using separate methods due to polarity differences between the compound classes and the incompatibility of EtG with enzymatic hydrolysis. In this method, barbiturates were incorporated into an existing EtG\/EtS method, providing testing labs with a potential avenue for a more efficient approach through simultaneous analysis. <\/p>\n\n\n\n

Introduction<\/h2>\n\n\n\n

Ethanol metabolites ethyl-\u03b2-D-glucuronide (EtG) and ethyl sulfate (EtS) are unique biomarkers of alcohol use. They are frequently monitored to ensure compliance with zero tolerance treatment programs and for abstinence enforcement, situations in which information regarding recent alcohol consumption is required. The analysis of these compounds is especially beneficial in these circumstances because they have a three-day detection window, are relatively stable when samples are stored correctly, and can be detected with high specificity. However, EtG and EtS are both polar, which makes them difficult to retain via reversed-phase chromatography.<\/p>\n\n\n\n

Barbiturates are also commonly monitored in urine as part of drug screening programs and, while they are not polar compounds, they are similar to EtG and EtS in that they are both analyzed in negative electrospray ionization (ESI) mode by LC-MS\/MS. But, due to polarity and sample preparation differences, the analysis of alcohol metabolites and barbiturates in the same urine sample usually is done using separate methods and LC columns with different stationary phases. Although, in both cases, the methods used must be able to reliably separate the target analytes from coeluting matrix components.<\/p>\n\n\n\n

Recently, interest has grown in developing a single negative ESI LC-MS\/MS method to analyze both barbiturates and alcohol metabolites in urine, but this presents a significant challenge because the enzymatic hydrolysis sample preparation that often is used for barbiturates would be detrimental to EtG analysis. In this hydrolysis reaction, \u03b2-glucuronidase is used to cleave the glucuronide-conjugated barbiturates and convert them into their parent compounds. But, this enzyme will also cleave the glucuronide moiety from EtG, causing it to break down into ethanol which ultimately reduces levels of EtG in urine and leads to inconclusive or inaccurate results.  <\/p>\n\n\n\n

In this study, a simple dilute-and-shoot LC-MS\/MS method was developed for the simultaneous analysis of alcohol metabolites and barbiturates in human urine without enzymatic hydrolysis. This method was developed on a Raptor EtG\/EtS column because it has previously been demonstrated to be highly effective at retaining EtG and EtS and separating them from matrix interferences [1]. Using the selectivity of a Raptor EtG\/EtS column, analysis of these two classes of compounds was combined into a single, hydrolysis-free method, which ultimately has the potential to increase productivity and reduce costs for labs currently running the same sample through separate methods.<\/p>\n\n\n\n

Experimental<\/h2>\n\n\n\n

Calibration Standards and Quality Control Samples<\/em><\/h3>\n\n\n\n

Human urine (alcohol and barbiturates free) was fortified with EtG, EtS, phenobarbital, butalbital, amobarbital, pentobarbital, and secobarbital in order to prepare the calibration standards and QC samples. The calibration standards concentration range was 50\u20135,000 ng\/mL for all the analytes. Four QC levels were prepared at 50, 125, 750, and 4,000 ng\/mL.<\/p>\n\n\n\n

Sample Preparation<\/em><\/h3>\n\n\n\n

50 \u00b5L of urine was diluted with 950 \u00b5L of the working internal standard (100 ng\/mL EtG-d5, EtS-d5, and barbiturates-d5 in 0.1% formic acid in water).  Samples were vortexed at 3500 rpm for ten seconds to mix, followed by centrifugation at 3000 rpm for five minutes at 10 \u00b0C and then injected for LC-MS\/MS analysis. Additional urine double blanks were prepared and injected for column equilibration.<\/p>\n\n\n\n

Chromatographic Method:<\/em><\/h3>\n\n\n\n

The chromatographic conditions used on a Shimadzu Prominence HPLC connected to a Sciex 4000 for this LC-MS\/MS analysis of alcohol metabolites and barbiturates in urine are detailed below. The ion transitions used for each analyte are provided in Table I. Running 30 matrix injections through the full gradient program is recommended for equilibrating the column [2].<\/p>\n\n\n

\n\n\n\n\n\n\n\n\n\n\n\n\n
Column:<\/td>\nRaptor EtG\/EtS 2.7 \u00b5m, 100 mm x 2.1 mm (cat.# 9325A12)<\/td>\n<\/tr>\n
Guard column:<\/td>\nUltraShield UHPLC precolumn filter 0.2 \u00b5m (cat.# 25809)<\/td>\n<\/tr>\n
Sample temp.:<\/td>\n10 \u00b0C<\/td>\n<\/tr>\n
Column temp.:<\/td>\n30 \u00b0C<\/td>\n<\/tr>\n
Injection volume:<\/td>\n10 \u00b5L<\/td>\n<\/tr>\n
Mobile phase A:<\/td>\n0.1% Formic acid in water<\/td>\n<\/tr>\n
Mobile phase B:<\/td>\n0.1% Formic acid in acetonitrile<\/td>\n<\/tr>\n
 <\/td>\nTime (min) %B 0.00 5 1.20 20 1.21 35 3.00 45 4.00 5 5.00 stop<\/td>\n<\/tr>\n
Flow rate:<\/td>\n0.5 mL\/min<\/td>\n<\/tr>\n
Ion mode:<\/td>\nNegative ESI<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n\n\n
Table I:<\/strong> Ion Transitions for LC-MS\/MS Analysis of Alcohol Metabolites and Barbiturates<\/h5>\n\n\n\n
Peak Identification<\/strong><\/td>Precursor <\/strong>Ion<\/strong><\/td>Quantitative <\/strong>Ion<\/strong><\/td>Qualitative <\/strong>Ion<\/strong><\/td><\/tr>
Ethyl-\u03b2-D-glucuronide-d5 (EtG-d5)<\/td>225.9<\/td>84.7<\/td>–<\/td><\/tr>
Ethyl-\u03b2-D-glucuronide (EtG)<\/td>220.9<\/td>74.9<\/td>85.0<\/td><\/tr>
Ethyl sulfate-d5 (EtS-d5)<\/td>129.7<\/td>97.7<\/td>–<\/td><\/tr>
Ethyl sulfate (EtS)<\/td>124.8<\/td>96.8<\/td>79.7<\/td><\/tr>
Phenobarbital-d5<\/td>236.0<\/td>42.0<\/td>–<\/td><\/tr>
Phenobarbital<\/td>231.2<\/td>188.0<\/td>42.0<\/td><\/tr>
Butalbital-d5<\/td>228.0<\/td>42.0<\/td>–<\/td><\/tr>
Butalbital<\/td>223.0<\/td>180.0<\/td>42.0<\/td><\/tr>
Amobarbital-d5<\/td>230.0<\/td>42.0<\/td>–<\/td><\/tr>
Pentobarbital-d5<\/td>230.0<\/td>42.0<\/td>–<\/td><\/tr>
Amobarbital-<\/td>225.0<\/td>182.0<\/td>42.0<\/td><\/tr>
Pentobarbital<\/td>225.0<\/td>182.0<\/td>42.0<\/td><\/tr>
Secobarbital-d5<\/td>242.0<\/td>42.0<\/td>–<\/td><\/tr>
Secobarbital<\/td>237.0<\/td>194.0<\/td>42.0<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Results and Discussion<\/h2>\n\n\n\n

Chromatographic Performance<\/em><\/h3>\n\n\n\n

For this work, a previously published, dilute-and-shoot EtG and EtS method [1] was expanded to include the barbiturates by making simple changes to the mobile phase gradient in the original method. As shown in Figure 1, a fast 5-minute analysis of alcohol metabolites and barbiturates was successfully obtained from the direct injection of urine sample supernatant without using enzymatic hydrolysis. All target compounds eluted within three minutes, and the total cycle time was extended to five minutes to allow for column re-equilibration.<\/p>\n\n\n\n

The selectivity of the Raptor EtG\/EtS column provided ample chromatographic separation of the target analytes from isobaric matrix interferences, making peak identification easy for most compounds. However, amobarbital and pentobarbital could not be separated from each other under these method conditions. These isobaric barbiturates had good sensitivity and peak shape, but because they coelute, accurate quantitation using this method is only possible if just one of the two compounds is present in the sample. Therefore, if individual quantitative results are required for amobarbital and pentobarbital, a separate confirmatory method, such as Restek\u2019s barbiturate drug panel<\/a> [3], will be needed. While a confirmatory method would be required for definitive identification and individual quantitation, the current method is suitable for screening purposes.<\/p>\n\n\n\n

Column Robustness<\/em><\/h3>\n\n\n\n

Following 100 sample injections, all chromatographic peaks maintained their initial peak shape, retention time, and intensity (Figure 2). The maximum system pressure also remained at the same level indicating no column clogging had occurred.<\/p>\n\n\n\n

Linearity<\/em><\/h3>\n\n\n\n

Using linear 1\/x weighted regression, good linearity results were obtained with r2<\/sup> values of 0.992 or greater (Figure 3).<\/p>\n\n\n\n

Accuracy and Precision<\/em><\/h3>\n\n\n\n

Precision and accuracy analyses were performed on three different days. Method accuracy was demonstrated by percent recovery values being within 15% (20% for the LLOQ level) of the nominal concentration on all three days. The %RSD was 1.10-12.5% and 0.8-13.7% for intra- and interday comparisons, respectively, across the LLOQ, low, mid, and high QC levels indicating good method precision (Table II).<\/p>\n\n\n

Figure 1:<\/strong>\u00a0Analysis of Alcohol Metabolites and Barbiturates in Fortified Human Urine (500 ng\/mL Calibration Standard)<\/div>
\n
\"Simultaneous<\/div>

LC_CF0773<\/p>

Peaks<\/h4>\n
<\/th>Peaks<\/th>tR<\/sub> (min)<\/th>Conc.
(ng\/mL)<\/th>		Precursor Ion<\/th>Product Ion 1<\/th>Product Ion 2<\/th><\/tr><\/thead>\n
1.<\/td>EtG-d5<\/td>0.76<\/td>100<\/td>225.9<\/td>84.7<\/td>–<\/td><\/tr>\n
2.<\/td>EtG<\/a><\/td>0.80<\/td>500<\/td>220.9<\/td>74.9<\/td>85<\/td><\/tr>\n
3.<\/td>EtS-d5<\/td>1.70<\/td>100<\/td>129.7<\/td>97.7<\/td>–<\/td><\/tr>\n
4.<\/td>EtS<\/a><\/td>1.78<\/td>500<\/td>124.8<\/td>96.8<\/td>79.7<\/td><\/tr>\n
5.<\/td>Phenobarbital-d5<\/td>2.54<\/td>100<\/td>236.0<\/td>42.0<\/td>–<\/td><\/tr>\n
6.<\/td>Phenobarbital<\/a><\/td>2.55<\/td>500<\/td>231.2<\/td>188.0<\/td>42.0<\/td><\/tr>\n
7.<\/td>Butalbital-d5<\/td>2.57<\/td>100<\/td>228<\/td>42.0<\/td>–<\/td><\/tr>\n<\/tbody><\/table>\n\n
<\/th>Peaks<\/th>tR<\/sub> (min)<\/th>Conc.
(ng\/mL)<\/th>		Precursor Ion<\/th>Product Ion 1<\/th>Product Ion 2<\/th><\/tr><\/thead>
8.<\/td>Butalbital<\/a><\/td>2.58<\/td>500<\/td>223<\/td>180.0<\/td>42.0<\/td><\/tr>\n
9.<\/td>Amobarbital-d5<\/td>2.74<\/td>100<\/td>230<\/td>42.0<\/td>–<\/td><\/tr>\n
10.<\/td>Pentobarbital-d5<\/td>2.74<\/td>100<\/td>230<\/td>42.0<\/td>–<\/td><\/tr>\n
11.<\/td>Amobarbital<\/a><\/td>2.75<\/td>500<\/td>225<\/td>182.0<\/td>42.0<\/td><\/tr>\n
12.<\/td>Pentobarbital<\/a><\/td>2.76<\/td>500<\/td>225<\/td>182.0<\/td>42.0<\/td><\/tr>\n
13.<\/td>Secobarbital-d5<\/td>2.93<\/td>100<\/td>242<\/td>42.0<\/td>–<\/td><\/tr>\n
14.<\/td>Secobarbital<\/a><\/td>2.93<\/td>500<\/td>237<\/td>194.0<\/td>42.0<\/td><\/tr>\n<\/tbody><\/table><\/div>
<\/path><\/g><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n
Figure 2:\u00a0<\/strong>Lifetime Testing: Raptor EtG\/EtS Provide Consistent Results Even After 100 Sample Injections<\/div>
\n
\"Raptor<\/div>

LC_CF0775<\/p>

Peaks<\/h4>\n
<\/th>Peaks<\/th>tR<\/sub> (min)<\/th>Conc.
(ng\/mL)<\/th>		Precursor Ion<\/th>Product Ion.1<\/th>Product Ion.2<\/th><\/tr><\/thead>\n
1.<\/td>EtG-d5<\/td>0.76<\/td>100<\/td>225.9<\/td>84.7<\/td>–<\/td><\/tr>\n
2.<\/td>EtG<\/a><\/td>0.80<\/td>500<\/td>220.9<\/td>74.9<\/td>85<\/td><\/tr>\n
3.<\/td>EtS-d5<\/td>1.70<\/td>100<\/td>129.7<\/td>97.7<\/td>–<\/td><\/tr>\n
4.<\/td>EtS<\/a><\/td>1.78<\/td>500<\/td>124.8<\/td>96.8<\/td>79.7<\/td><\/tr>\n
5.<\/td>Phenobarbital-d5<\/td>2.54<\/td>100<\/td>236.0<\/td>42.0<\/td>–<\/td><\/tr>\n
6.<\/td>Phenobarbital<\/a><\/td>2.55<\/td>500<\/td>231.2<\/td>188.0<\/td>42.0<\/td><\/tr>\n
7.<\/td>Butalbital-d5<\/td>2.57<\/td>100<\/td>228<\/td>42.0<\/td>–<\/td><\/tr>\n<\/tbody><\/table>\n\n
<\/th>Peaks<\/th>tR<\/sub> (min)<\/th>Conc.
(ng\/mL)<\/th>		Precursor Ion<\/th>Product Ion.1<\/th>Product Ion.2<\/th><\/tr><\/thead>
8.<\/td>Butalbital<\/a><\/td>2.58<\/td>500<\/td>223<\/td>180.0<\/td>42.0<\/td><\/tr>\n
9.<\/td>Amobarbital-d5<\/td>2.74<\/td>100<\/td>230<\/td>42.0<\/td>–<\/td><\/tr>\n
10.<\/td>Pentobarbital-d5<\/td>2.74<\/td>100<\/td>230<\/td>42.0<\/td>–<\/td><\/tr>\n
11.<\/td>Amobarbital<\/a><\/td>2.75<\/td>500<\/td>225<\/td>182.0<\/td>42.0<\/td><\/tr>\n
12.<\/td>Pentobarbital<\/a><\/td>2.76<\/td>500<\/td>225<\/td>182.0<\/td>42.0<\/td><\/tr>\n
13.<\/td>Secobarbital-d5<\/td>2.93<\/td>100<\/td>242<\/td>42.0<\/td>–<\/td><\/tr>\n
14.<\/td>Secobarbital<\/a><\/td>2.93<\/td>500<\/td>237<\/td>194.0<\/td>42.0<\/td><\/tr>\n<\/tbody><\/table><\/div>
<\/path><\/g><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n
Figure 3:<\/strong>\u00a0Calibration Curves<\/div>
\n\n

EtG<\/strong><\/p>\n\n\n

\n
\"\"<\/figure>\n<\/div>\n\n\n

EtS<\/strong><\/p>\n\n\n

\n
\"\"<\/figure>\n<\/div>\n\n\n

Phenobarbital<\/strong><\/strong><\/p>\n\n\n

\n
\"\"<\/figure>\n<\/div>\n\n\n

Butalbital<\/strong><\/strong><\/p>\n\n\n

\n
\"\"<\/figure>\n<\/div>\n\n\n

Secobarbital<\/strong><\/p>\n\n\n

\n
\"\"<\/figure>\n<\/div>\n<\/div><\/div><\/div>\n\n\n
<\/div>\n\n\n\n
Table II:<\/strong> Interday Accuracy and Precision of QC Samples*<\/h5>\n\n\n\n
Analyte<\/strong><\/td>Avg. Conc. (ng\/mL)<\/strong><\/td>Avg. Accuracy (%)<\/strong><\/td>%RSD<\/strong><\/td><\/tr>
QC LLOQ (50 ng\/mL)<\/strong><\/td><\/tr>
Ethyl-\u03b2-D-glucuronide (EtG)<\/td>55.0<\/td>110<\/td>7.70<\/td><\/tr>
Ethyl sulfate (EtS)<\/td>54.3<\/td>109<\/td>13.7<\/td><\/tr>
Phenobarbital<\/td>59.7<\/td>119<\/td>9.60<\/td><\/tr>
Butalbital<\/td>58.3<\/td>117<\/td>13.2<\/td><\/tr>
Secobarbital<\/td>43.0<\/td>86.0<\/td>6.25<\/td><\/tr>
QC Low (125 ng\/mL)<\/strong><\/td><\/tr>
Ethyl-\u03b2-D-glucuronide (EtG)<\/td>123<\/td>98.4<\/td>4.10<\/td><\/tr>
Ethyl sulfate (EtS)<\/td>125<\/td>100<\/td>0.800<\/td><\/tr>
Phenobarbital<\/td>136<\/td>109<\/td>5.80<\/td><\/tr>
Butalbital<\/td>128<\/td>102<\/td>5.50<\/td><\/tr>
Secobarbital<\/td>131<\/td>104<\/td>4.20<\/td><\/tr>
QC Mid (700 ng\/mL)<\/strong><\/td><\/tr>
Ethyl-\u03b2-D-glucuronide (EtG)<\/td>729<\/td>104<\/td>11.3<\/td><\/tr>
Ethyl sulfate (EtS)<\/td>669<\/td>95.6<\/td>6.10<\/td><\/tr>
Phenobarbital<\/td>723<\/td>103<\/td>8.40<\/td><\/tr>
Butalbital<\/td>659<\/td>94.1<\/td>3.50<\/td><\/tr>
Secobarbital<\/td>676<\/td>96.5<\/td>7.20<\/td><\/tr>
QC High (4000 ng\/mL)<\/strong><\/td><\/tr>
Ethyl-\u03b2-D-glucuronide (EtG)<\/td>4262<\/td>107<\/td>5.90<\/td><\/tr>
Ethyl sulfate (EtS)<\/td>4090<\/td>102<\/td>3.60<\/td><\/tr>
Phenobarbital<\/td>4003<\/td>101<\/td>8.50<\/td><\/tr>
Butalbital<\/td>3987<\/td>99.7<\/td>6.50<\/td><\/tr>
Secobarbital<\/td>3720<\/td>93.0<\/td>8.40<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

*While good peak shape and sensitivity were obtained, individual results for amobarbital and pentobarbital could not be reported because these isobaric compounds were not chromatographically separated. <\/p>\n\n\n\n

<\/div>\n\n\n\n

Conclusion<\/h2>\n\n\n\n

It was demonstrated that the Raptor EtG\/EtS column provides excellent performance for the simultaneous analysis of alcohol metabolites and barbiturates in human urine. Isobaric matrix interferences were easily resolved, preventing issues with peak identification and quantitation, even though only minimal sample preparation (dilute-and-shoot) was used. With a simple sample preparation procedure (no enzymatic hydrolysis) and 5-minute analysis time, this procedure can provide accurate, high-throughput monitoring of alcohol and barbiturates in urine. The single negative ESI LC-MS\/MS method demonstrated here combines panels for higher sample throughput; however, it is important to note that EtG samples cannot undergo enzymatic hydrolysis, so laboratories considering adopting this method must independently determine the viability of analyzing their barbiturate samples without enzymatic hydrolysis.<\/p>\n\n\n\n

<\/div>\n\n\n\n

References<\/h2>\n\n\n\n
    \n
  1. J. Steimling, R. Alagandula, and F. Carroll, Successful strategies for the analysis of EtG and EtS in urine: rugged sample preparation and analysis conditions for high-throughput labs<\/a>, Restek Corporation, 2020 <\/li>\n\n\n\n
  2. Tech tip: column conditioning ensures consistent EtG\/EtS results<\/a>, Restek Corporation, 2017 <\/li>\n\n\n\n
  3. Barbiturate drug panel on Raptor C18 by LC-MS\/MS<\/a>, Restek Corporation<\/li>\n<\/ol>\n\n\n\n
    <\/div>\n\n\n
    \n

    Products Mentioned<\/h3>\n
    \n
    \n
    \n
    \n Catalog No. 9325A12 <\/a> <\/div>\n
    \n
    Colonne Raptor EtG\/EtS, 2.7 \u00b5m, Colonne LC 100 x 2.1 mm<\/div>\n <\/div>\n
    \n Voir le produit<\/a>\n <\/div>\n <\/div>\n
    \n
    \n Catalog No. 25809 <\/a> <\/div>\n
    \n
    Filtre\/pr\u00e9colonne UltraShield UHPLC, fritt\u00e9 0.2 \u03bcm, l\u2019unit\u00e9<\/div>\n <\/div>\n
    \n Voir le produit<\/a>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n \n\n\n
    <\/div>\n","protected":false},"excerpt":{"rendered":"

    This hydrolysis-free method for the simultaneous analysis of alcohol metabolites and barbiturates allows all target analytes to be reported from a single LC-MS\/MS method.<\/p>\n","protected":false},"author":67,"featured_media":89952,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[784],"tags":[],"industries-application":[2223,2239],"post-badge":[],"resource-type":[],"product-library":[2499,2481],"resource-technique":[2326,2363],"hf_cat_post":[651],"ppma_author":[604],"class_list":["post-43356","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-application-notes-it","industries-application-klinisch","industries-application-forensik","product-library-lc-saulen","product-library-flussigchromatographie-produkte","resource-technique-flussigchromatografie","resource-technique-ms-ms-de"],"acf":[],"taxonomy_info":{"category":[{"value":784,"label":"Application Notes"}],"industries-application":[{"value":2223,"label":"Klinisch"},{"value":2239,"label":"Forensik"}],"product-library":[{"value":2499,"label":"LC S\u00e4ulen"},{"value":2481,"label":"Fl\u00fcssigchromatographie Produkte"}],"resource-technique":[{"value":2326,"label":"Fl\u00fcssigchromatografie"},{"value":2363,"label":"MS\/MS"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-cfan3719-1024x536.jpg",1024,536,true],"author_info":{"display_name":"MariAnn Wall","author_link":"https:\/\/discover.restek.com\/fr\/author\/mariann-wall\/"},"comment_info":0,"category_info":[{"term_id":784,"name":"Application Notes","slug":"application-notes-it","term_group":0,"term_taxonomy_id":784,"taxonomy":"category","description":"","parent":0,"count":86,"filter":"raw","cat_ID":784,"category_count":86,"category_description":"","cat_name":"Application Notes","category_nicename":"application-notes-it","category_parent":0}],"tag_info":false,"authors":[{"term_id":604,"user_id":0,"is_guest":1,"slug":"ravali-alagandula","display_name":"Ravali Alagandula","avatar_url":"https:\/\/secure.gravatar.com\/avatar\/?s=96&d=mm&r=g","0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/43356","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/users\/67"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/comments?post=43356"}],"version-history":[{"count":10,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/43356\/revisions"}],"predecessor-version":[{"id":74238,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/posts\/43356\/revisions\/74238"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/media\/89952"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/media?parent=43356"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/categories?post=43356"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/tags?post=43356"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/industries-application?post=43356"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/post-badge?post=43356"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/resource-type?post=43356"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/product-library?post=43356"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/resource-technique?post=43356"},{"taxonomy":"hf_cat_post","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/hf_cat_post?post=43356"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/fr\/wp-json\/wp\/v2\/ppma_author?post=43356"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}